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排序方式: 共有75条查询结果,搜索用时 15 毫秒
51.
Alexander Smith Camilo A. Beltrán Manabu Kusunoki Alba M. Cotes Keiichi Motohashi Takumasa Kondo Michihito Deguchi 《Journal of General Plant Pathology》2013,79(1):74-85
Twenty-one isolates of Trichoderma spp. were collected from eight states in Colombia and characterized based on the 5′ end of the translation elongation factor-1α (EF1-α1) gene and RNA polymerase II gene encoding the second largest protein subunit (RPB2) by using mixed primers. Seven species of soil-dwelling Trichoderma were found: T. atroviride, T. koningiopsis, T. asperellum, T. spirale, T. harzianum, T. brevicompactum and T. longibrachiatum. Species identifications based on the EF1-α1 gene were consistent with those obtained from the RPB2 gene. Phylogenetic analyses with high bootstrap values supported the validity of the identification of all isolates. These results suggest that using the combination of the genes EF1-α1 and RPB2 is highly reliable for molecular characterization of Trichoderma species. Trichoderma asperellum Th034, T. atroviride Th002 and T. harzianum Th203 prevented germination of more than 70 % of sclerotia of Sclerotinia sclerotiorum in bioassay tests and are promising biological control agents. No relationship between mycelium growth rate and parasitism level was found. 相似文献
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A single dilution enzyme-linked immunosorbent assay for the quantitative detection of antibodies to bovine herpesvirus type 1 总被引:1,自引:0,他引:1
Three serological assays were compared for detection of antibodies to bovine herpes-virus type 1. These were virus neutralization (VN), enhanced complement fixation (CF) and enzyme-linked immunosorbent assay (ELISA). The ELISA was developed using an infected cell lysate antigen and purified virus and was optimized in relation to antigen and antisera dilutions. The CF assay was enhanced by the addition of bovine complement. These 3 assays were compared for detection of: specific virus antibody titers; sero-conversions; early antibody response in experimentally-infected cattle. Both ELISA end-point titers and single dilution values were found to be more sensitive than the CF or VN assays for specific antibody level quantitation. With a single dilution ELISA test procedure a correlation was obtained between ELISA values and VN titers. Using the single dilution ELISA test the assay also detected antibodies in experimentally-infected cattle before either the VN or CF assays, and agreed with the VN test in 35/38 seroconversions found by 4-fold or more VN changes between acute and convalescent paired sera from naturally-infected animals. The single dilution ELISA was a rapid and sensitive test for routine antibody detection in bovine sera. 相似文献
54.
By the halothane test we determined the development of the syndrome of malignant hyperthermia in pigs of the Belgian Landrace breed with body weight of 20 kg. We found that from 27 animals that were subjected to the test 12 positively reacted to Narcotan - Spofa anaesthesis. Pigs with the syndrome of malignant hyperthermia had considerably higher rectal temperature after anaesthesis, lower PH of venose blood, higher concentration of lactic acid and glucosis in the blood plasma. Halothane-negative animals did not produce and conspicuous changes of these indices. Most differences were statisticaly highly significant. 相似文献
55.
Mark D. Needham Brian W. Szuster Laura Lesar Camilo Mora Daniel P. Knecht 《Human Dimensions of Wildlife》2018,23(5):461-473
This research note examined encounters, norms, crowding, satisfaction, and displacement among people snorkeling and scuba diving with manta rays at sites in Hawaii. These sites are popular with up to 30 tour boats and 300 participants each day. Data from a survey of 444 participants showed that 82% felt crowded by snorkelers, 78% felt crowded by boats, and 69% felt crowded by scuba divers when viewing manta rays. In reporting their norms, participants stated they would accept seeing an average of no more than 52 snorkelers, 32 scuba divers, and 11 boats at one time. However, 77% of respondents encountered more snorkelers than their norm for seeing snorkelers, 67% saw more scuba divers than they would accept, and 68% encountered more boats than their norm. These participants were more crowded, less satisfied, and more likely to become displaced (not visit again) compared to those who encountered fewer than their norms. 相似文献
56.
Amanuel G. Ghebretinsae Shirley A. Graham Gerardo R. Camilo Janet C. Barber 《Industrial Crops and Products》2008,27(3):279-287
Fatty acid analyses of seed lipids were performed for 28 populations representing three widely distributed Cuphea species. Locality and climatic data for all samples were also compiled. The objectives of this study were to examine the extent of variation in seed oil composition among the wild populations, and to discover any patterns of relationship between fatty acid composition of seed oils and environmental factors. While we found the dominant fatty acid in the oil of each species remained consistent over the environmental and geographical ranges of the samples in the present study, variation as high as 30.6% in the amount of the dominant fatty acid produced was observed. Correlation analysis between fatty acid composition and the individual environmental factors of latitude, elevation or temperature showed no consistent pattern of influence. However, when considered together, the interaction of all three, and especially latitude and elevation, contributed significantly to the variation among populations. Environmental data at microhabitat level and through controlled environmental experiments will be needed for more precise understanding of factors affecting Cuphea seed oil composition at the population level. 相似文献
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Speisky H López-Alarcón C Gómez M Fuentes J Sandoval-Acuña C 《Journal of agricultural and food chemistry》2012,60(36):8851-8859
This paper reports the first database on antioxidants contained in fruits produced and consumed within the south Andes region of South America. The database ( www.portalantioxidantes.com ) contains over 500 total phenolics (TP) and ORAC values for more than 120 species/varieties of fruits. All analyses were conducted by a single ISO/IEC 17025-certified laboratory. The characterization comprised native berries such as maqui ( Aristotelia chilensis ), murtilla ( Ugni molinae ), and calafate ( Barberis microphylla ), which largely outscored all other studied fruits. Major differences in TP and ORAC were observed as a function of the fruit variety in berries, avocado, cherries, and apples. In fruits such as pears, apples, apricots, and peaches, a significant part of the TP and ORAC was accounted for by the antioxidants present in the peel. These data should be useful to estimate the fruit-based intake of TP and, through the ORAC data, their antioxidant-related contribution to the diet of south Andes populations. 相似文献
59.
Vicente SJ Ishimoto EY Cruz RJ Pereira CD Torres EA 《Journal of agricultural and food chemistry》2011,59(20):10887-10892
This study evaluated the acute effect of the administration of coffee brew in the activity of phase II antioxidant enzymes in the hepatic tissue of rats. A single dose of this beverage increased the activity of the enzymes SOD, CAT, and GPx; the maximum increase occurred 1 h after administration (19.1, 22.1, and 25.1%, respectively). These changes were statistically significant (p < 0.05), the response was shown to be dose-dependent (p < 0.05), and the return to basal levels took >4 h from the intervention, suggesting a long-term effect. The total antioxidant capacity of the hepatic tissue also exhibited a peak 1 h after the intervention (6.5%), but the increase was not statistically significant and the response was not dose-dependent due to the low exposure to coffee. These results indicate that coffee increases the activities of antioxidant enzymes, improving protection against oxidative stress. 相似文献
60.