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排序方式: 共有3158条查询结果,搜索用时 15 毫秒
61.
Waseem Akbar Anilkumar Gowda Jeffrey E Ahrens Jason W Stelzer Robert S Brown Scott L Bollman John T Greenplate Jeffrey Gore Angus L Catchot Gus Lorenz Scott D Stewart David L Kerns Jeremy K Greene Michael D Toews David A Herbert Dominic D Reisig Gregory A Sword Peter C Ellsworth Larry D Godfrey Thomas L Clark 《Pest management science》2019,75(3):867-877
62.
Between‐site and ‐year variation in the relative susceptibility of native Scottish Pinus sylvestris populations to dothistroma needle blight 下载免费PDF全文
Dothistroma needle blight (DNB), caused by Dothistroma septosporum, is currently the disease causing most concern in British pine plantations. Previous artificial inoculation (AI) experiments showed that native Scottish Scots pine (Pinus sylvestris) populations vary in susceptibility to DNB. However, it is unclear if the relative susceptibility of Scots pine populations observed in these experiments can be replicated under natural conditions. It is also unknown whether relative susceptibility of Scots pine populations varies between sites or years. To answer these two questions, young Scots pine plants from six native Scottish populations (Abernethy, Allt Broighleachan, Amat, Beinn Eighe, Glen Cannich and Glen Loyne) were exposed to natural D. septosporum inoculum at two Scottish sites (Culbin and Torrs Warren) between 2012 and 2014. DNB disease incidence and severity was assessed each October. Relative susceptibilities of the Scots pine populations varied between sites and across years. In two of the three years at Torrs Warren (2012 and 2014), the relative susceptibilities of the populations were strongly positively correlated with those observed in previous AI experiments. In these years, trees from Glen Loyne and Glen Cannich were the most susceptible. Conversely, there was no correlation between the relative susceptibilities seen in any year at Culbin with those observed in AI experiments. At Culbin, Beinn Eighe was the most susceptible population. Across both sites, there was a strong positive relationship between total summer precipitation and DNB severity (R = 0·93, t = 8·2, P = 0·001). 相似文献
63.
A reconsideration of natural dispersal distances of the pine pathogen Dothistroma septosporum 下载免费PDF全文
The natural spread of Dothistroma septosporum, the causal agent of a foliar disease of pines, was investigated at three sites in the south of England using trap plants. The pathogen is considered to be primarily rain‐splash dispersed, but this study shows that it can be spread many hundreds of metres from an inoculum source, demonstrating that dispersal is not solely via rain splash. The maximum distance the pathogen was recorded from any infection source was in excess of 1400 m, over five times the distance defined in the only previous work of this kind. Consequently, a reassessment of forest and production nursery management practices is called for, as these assume that the pathogen only spreads naturally over limited distances. Detection of the pathogen on trap plants over 100 m from the inoculum source was, in most cases, only possible using quantitative real‐time PCR diagnosis. The entire diagnostic procedure, from DNA extraction to amplification, was able to detect a minimum of approximately 17 D. septosporum cells in a pine needle sample, assuming only a moderate DNA extraction efficiency of 30%. 相似文献
64.
65.
Guanine nucleotide binding (G) proteins (subunit composition alpha beta gamma) dissociate on activation with guanosine triphosphate (GTP) analogs and magnesium to give alpha-guanine nucleotide complexes and free beta gamma subunits. Whether the opening of potassium channels by the recently described Gk in isolated membrane patches from mammalian atrial myocytes was mediated by the alpha k subunit or beta gamma dimer was tested. The alpha k subunit was found to be active, while the beta gamma dimer was inactive in stimulating potassium channel activity. Thus, Gk resembles Gs, the stimulatory regulatory component of adenylyl cyclase, and transducin, the regulatory component of the visual system, in that it regulates its effector function--the activity of the ligand-gated potassium channel--through its guanine nucleotide binding subunit. 相似文献
66.
Pettengill GH Ford PG Brown WE Kaula WM Masursky H Eliason E McGill GE 《Science (New York, N.Y.)》1979,205(4401):90-93
Three large Venus surface features, identified previously in images obtained from Earth-based radar observations, are shown by the Pioneer Venus radar mapper to be elevated 5 to 10 kilometers above the surrounding terrain. Two of these features, one bright and the other dark, lie adjacent to each other astride the 65 degrees N parallel between longitudes 310 degrees E and 10 degrees E. The combined region forms a huge tectonically uplifted plateau, surmounted by radar-bright ridges that may have either a volcanic or tectonic origin. The third feature, located at 30 degrees N, 283 degrees E, is radar-bright and may consist of volcanic material extruded along a fault zone. A first radar-scattering image, compiled from data obtained by the mapper in its imaging mode, shows a region north of the equator; several circular depressions seen in this area may result from meteoritic impact. 相似文献
67.
Development and characterization of a technique for percutaneous radiologic gastrojejunostomy tube placement in the dog 下载免费PDF全文
68.
JS Munday H Bentall D Aberdein M Navarro FA Uzal S Brown 《New Zealand veterinary journal》2020,68(4):242-246
ABSTRACTCase history and clinical findings: A flock of 20 sheep was kept within three paddocks on a single property. None of the animals in the flock had been vaccinated against any disease for at least three years. Abdominal bloating and haemorrhagic diarrhoea were observed in Lamb 1 at 24 hours-of-age. The lamb subsequently died within an hour of the onset of clinical signs. Lamb 2 was 3-days-old when observed to be recumbent with opisthotonus. The lamb was treated with dextrose, vitamins B1 and B12, and penicillin G, but died 4 hours later.Pathological findings: Examination of Lamb 1 revealed markedly increased gas within the peritoneum and within dilated loops of intestine. The intestines were dark red and contained large quantities of haemorrhagic fluid. Histology of the intestines revealed peracute mucosal necrosis with minimal accompanying inflammation. The intestinal lumen contained cell debris, haemorrhage, and myriad large Gram-positive bacilli. The intestines of Lamb 2 did not appear bloated or reddened. However, multiple fibrin clots were visible within the pericardial sac. Histopathological examination revealed small foci of necrosis within the mucosa of the distal intestine. The necrotic foci were often associated with large numbers of large Gram-positive bacilli.Immunohistochemsitry and molecular biology: Intestinal samples from Lamb 1 were processed for Clostridium perfringens immunohistochemistry, which revealed large numbers of intralesional, positively immunostained rods. Fragments corresponding to the expected sizes for genes encoding alpha, beta, and epsilon C. perfringens typing toxins were amplified by PCR from DNA extracted from formalin-fixed sections of intestine.Diagnosis: Lamb dysentery due to C. perfringens type B.Clinical relevance: C. perfringens bacteria have a worldwide distribution, but disease due to C. perfringens type B has only been diagnosed in a small number of countries and has never been reported in New Zealand or Australia. C. perfringens type B produce both beta toxin and epsilon toxins, therefore both haemorrhagic enteritis and systemic vascular damage can develop. As many animals are exposed to C. perfringens without developing disease, there must be additional unknown factors that resulted in disease in these particular sheep. Vaccines that specifically protect against C. perfringens type B are available and may be recommended for use in smaller non-commercial flocks, as in the present case. 相似文献
69.
R. G. Novy J. L. Whitworth J. C. Stark B. A. Charlton S. Yilma N. R. Knowles M. J. Pavek R. R. Spear T. L. Brandt N. Olsen M. Thornton C. R. Brown 《American Journal of Potato Research》2014,91(4):380-393
Teton Russet is an early-maturing, medium-russeted, potato cultivar with high merit for both fresh-pack and processing. In early harvest trials in the Pacific Northwest, Teton Russet had total yields similar to Russet Norkotah, and higher than Ranger Russet and Russet Burbank. Marketable yield of Teton Russet in the early harvest trials was also comparable to or higher than Russet Norkotah in Washington and Oregon, and higher than Ranger Russet and Russet Burbank at these sites, as well as in Idaho. In full-season trials, while total yield of the earlier-maturing Teton Russet tended to be lower than Ranger Russet and Russet Burbank, marketable yield was generally higher than Russet Burbank across the majority of sites due to its higher percentage of U.S. No. 1 tubers. Teton Russet is suitable for processing, with acceptable fry color following up to 8 months of storage at 8.9 °C. Uniformity of fry color was also very consistent. Teton Russet has shown lower levels of the amino acid asparagine relative to Ranger Russet and Russet Burbank which may contribute to lower acrylamide levels in French fries and other processed potato products. Teton Russet is notable for having resistance to common scab (Streptomyces spp.) and Fusarium dry rot, and is moderately resistant to tuber net necrosis. Analyses have also shown Teton Russet to have significantly higher protein levels than Russet Norkotah, Ranger Russet, and Russet Burbank, as well as higher vitamin C content than Russet Norkotah and Russet Burbank. Teton Russet was released in 2011 by the USDA-ARS and the Agricultural Experiment Stations of Idaho, Oregon, and Washington, and is a product of the Pacific Northwest Potato Variety (Tri-State) Development Program. 相似文献
70.
Brown KN Chen S Han Z Lu CH Tan X Zhang XJ Ding L Lopez-Cruz A Saur D Anderson SA Huang K Shi SH 《Science (New York, N.Y.)》2011,334(6055):480-486
The neocortex contains excitatory neurons and inhibitory interneurons. Clones of neocortical excitatory neurons originating from the same progenitor cell are spatially organized and contribute to the formation of functional microcircuits. In contrast, relatively little is known about the production and organization of neocortical inhibitory interneurons. We found that neocortical inhibitory interneurons were produced as spatially organized clonal units in the developing ventral telencephalon. Furthermore, clonally related interneurons did not randomly disperse but formed spatially isolated clusters in the neocortex. Individual clonal clusters consisting of interneurons expressing the same or distinct neurochemical markers exhibited clear vertical or horizontal organization. These results suggest that the lineage relationship plays a pivotal role in the organization of inhibitory interneurons in the neocortex. 相似文献