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901.
Shaikh B Rummel N Gieseker C Serfling S Reimschuessel R 《Journal of veterinary pharmacology and therapeutics》2003,26(6):421-427
Metabolic and residue depletion profiles of albendazole (ABZ) and its major metabolites in three fish species, rainbow trout, tilapia and Atlantic salmon are reported. Based on these profiles, similarities (or dissimilarities) between species will determine the potential to group fish species. ABZ at 10 mg/kg body weight was incorporated into fish food formulated in a gelatin base or in gel capsule and fed as a single dose to six fish from each species. Rainbow trout were held three each in a partitioned 600-L tank. Tilapia and Atlantic salmon were housed in separate 20-L tanks. Samples of muscle with adhering skin were collected at 8, 12, 18, 24, 48, 72, and 96 h postdose from trout kept at 12 degrees C, at 4, 8, 12, 24, 48, 72, 96, 120, and 144 h postdose from tilapia kept at 25 degrees C and at 8, 14, 24, 48, 72, and 96 h postdose from Atlantic salmon kept at 15 degrees C. The samples were homogenized in dry ice and subjected to extraction and cleanup procedures. The final extracts were analyzed for parent drug ABZ and its major metabolites, albendazole sulfoxide (ABZ-SO), albendazole sulfone (ABZ-SO2) and albendazole aminosulfone using high-performance liquid chromatography with fluorescence detection. ABZ was depleted by 24 h in trout and tilapia and by 48 h in salmon; ABZ-SO, a pharmacologically active metabolite, was depleted by 48 h in tilapia, by 72 h in rainbow trout and was present until 96 h in salmon; and low levels of ABZ-SO2 and albendazole aminosulfone, both inactive metabolites, were detectable at least till 96 h in all three fish species. 相似文献
902.
Buchanan MF Carter WC Cowgill LM Hurley DJ Lewis SJ MacLeod JN Melton TR Moore JN Pessah I Roberson M Robertson TP Smith ML Vandenplas ML 《Journal of veterinary medical education》2005,32(1):72-78
Traditional methods of teaching intracellular biological processes and pathways use figures or flowcharts with the names of molecules linked with arrows. Many veterinary students, presented with such material, simply memorize the names or chemical structures of the molecules and are then likely to forget the material once the examination is completed. To address this problem, the authors designed, created, and field-tested new teaching media that incorporate realistic three-dimensional (3D) animations depicting the dynamic changes that occur in intracellular molecules during cellular activation. Testing found that veterinary students taught using traditional teaching media (e.g., lectures, handouts, textbooks) are proficient in memorizing the names and order of intracellular molecules but unable to appreciate the interactions between these elements or their spatial relationships within cells. In contrast, more than 90% of veterinary students taught using 3D animations not only recall the facts about the intracellular elements but also develop accurate mental images of the interactions among these molecules and their spatial relationships. These findings strongly suggest that the comprehension of complex biological processes by veterinary students can be enhanced by the use of dynamic 3D depictions of these processes in the classroom. 相似文献
903.
C. J. Pfeiffer 《Canadian journal of veterinary research》1967,31(5):135-138
In the present investigation 42 female ferrets were studied in regard to the influence of canine distemper in this species on gastric acid secretion. A total of fifteen naturally-infected and 27 non-infected ferrets were fasted and pylorus-ligated, and were either injected with corticosterone (10 or 50 mg/kg, s.c. one injection/day for 4 days) suspended in corn oil, injected with corn oil, on non-injected. Prior to autopsy blood samples were acquired for corticosterone analysis, and at autopsy the volume, pH, free and combined acidity of the gastric contents were evaluated. 相似文献
904.
Occurrence of Grapevine Pinot gris virus in Friuli Venezia Giulia (Italy): field monitoring and virus quantification by real‐time RT‐PCR 下载免费PDF全文
G. L. Bianchi F. De Amicis L. De Sabbata N. Di Bernardo G. Governatori F. Nonino G. Prete T. Marrazzo S. Versolatto C. Frausin 《EPPO Bulletin》2015,45(1):22-32
Since 2003 the presence of a new syndrome characterized by symptoms of stunting, chlorotic mottling, leaf deformation, reduced yields and quality has been reported in some white berry varieties of Vitis vinifera in Trentino‐Alto Adige and Friuli Venezia Giulia vineyards. The identification of a new virus, provisionally called Grapevine Pinot gris virus (GPGV), in a cv. Pinot gris vine suggested an association between this new syndrome and the virus presence (Giampetruzzi et al., 2012), however the contemporary presence of GPGV in both symptomatic and asymptomatic plants has still to be explained. In this work, a large‐scale monitoring over a 3‐year period (2012–14) of Friuli Venezia Giulia vineyards and nurseries has shown a widespread presence of GPGV in symptomatic plants and also in asymptomatic vines, even if at a slightly lower percentage. Quantitative analyses of the virus titer revealed a great variability in the viral content of both symptomatic and asymptomatic plants but the mean GPGV quantity in symptomatic vines was significantly higher than in asymptomatic plants. 相似文献
905.
The uptake and movement of a chemical into a wheat leaf has been simulated in a 5-compartment model. The compound is considered to be applied to the leaf as discrete droplets of solution, from which water evaporates at a uniform rate. Solute diffuses from the droplets through the plant cuticle into the epidermal cell wall, and from there either into and out of the cytoplasm and vacuole of the mesophyll cells or along cell walls to the xylem. Once in the xylem it is carried in the transpiration stream towards the tip of the leaf. Accurate values for the parameters required by the model have not yet been measured, but the model simulates in a qualitative manner the movement pattern observed for 1-methyl-2-pyridone. The results presented focus attention on the possible importance of membrane permeability in determining the phytotoxicity of xylem mobile pesticides. 相似文献
906.
907.
The ability to characterize disease of the respiratory tract accurately based on breathing pattern is helpful for the development of differential diagnoses and an efficient diagnostic plan and critical for the stabilization of patients in respiratory distress. Veterinary students do not have sufficient clinical experience to observe personally all types of respiratory diseases and their resultant abnormal breathing patterns. We developed a teaching tool, the animated breathing pattern videotape (ABV), to fill this gap. The ABV is a collection of video clips of small animal patients with normal and abnormal breathing patterns on a conventional videotape of approximately 20 minutes duration. Each video clip is shown for 20 to 40 seconds, followed by the same clip with superimposed animation of rib and diaphragm motion, followed by the initial clip again, without overlying animation. The ABV has since been used in teaching third-year veterinary students, interns, residents, practicing veterinarians, and veterinary technicians. Student evaluations and responses to questionnaires by interns, residents, practicing veterinarians, veterinary technicians, and peer reviewers have been uniformly positive. 相似文献
908.
Four monoclonal antibodies (mAbs) (9.49, 24.27, 46.71 and 179.57) were produced against Fasciola hepatica excretory-secretory products. Isotype analysis revelead the antibodies to be IgM, IgG3, IgG1, and IgM. In immunoblot assays, the mAbs recognized different antigenic polypeptides migrating between 29 and 180 kDa. Specificity of the mAbs was evaluated by ELISA against antigens of Fascioloides magna, Anoplocephala magna, Stichorchis subtriquetrus, Haemonchus contortus, sheep liver extract (SLE), bovine liver extract (BLE), bovine serum albumin (BSA), bovine viral diarrhea virus (BVDV), and Madin-Darby bovine kidney (MDBK) cells. Monoclonals 9.49 and 24.27 were specific, and reacted only with Fasciola hepatica antigens. However, mAb 46.71 cross-reacted with antigens of Fascioloides magna, A. magna, Stichorchis subtriquetrus, and H. contortus but not with SLE, BLE, BSA, BVDV or MDBK cells. Monoclonal antibody 179.57 cross-reacted with Fascioloides magna, A. magna, S. subtriquetrus, H. contortus, SLE, and BLE, but not with BSA, BVDV, or MDBK cells. 相似文献
909.
Pharmacokinetics of xylazine after 2-, 4-, and 6-hr durations of continuous rate infusions in horses
Klaus Hopster Lawrence R. Soma Xiaoqing Li Charlotte Hopster-Iversen Raymond C. Boston Bernd Driessen 《Journal of veterinary pharmacology and therapeutics》2020,43(6):557-564
Intravenous (i.v.) bolus administration of xylazine (XYL) (0.5 mg/kg) immediately followed by a continuous rate infusion (CRI) of 1 mg kg−1 hr−1 for 2, 4, and 6 hr produced immediate sedation, which lasted throughout the duration of the CRI. Heart rate decreased and blood pressure increased significantly (p > .05) in all horses during the first 15 min of infusion, both returned to and then remained at baseline during the duration of the infusion. Compartmental models were used to investigate the pharmacokinetics of XYL administration. Plasma concentration–time curves following bolus and CRI were best described by a one-compartment model. No differences were found between pharmacokinetic estimates of the CRIs for the fractional elimination rate constant (Ke), half-life (t1/2e), volume of distribution (Vd), and clearance (Cl). Median and range were 0.42 (0.15–0.97)/hr, 1.68 (0.87–4.52) hr, 5.85 (2.10–19.34) L/kg, and 28.7 (19.6–39.5) ml min−1 kg−1, respectively. Significant differences were seen for area under the curve ( ) (p < .0002) and maximum concentration (Cmax) (p < .04). This indicates that with increasing duration of infusion, XYL may not accumulate in a clinically relevant way and hence no adjustments are required in a longer XYL CRI to maintain a constant level of sedation and a rapid recovery. 相似文献
910.