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53.
Habitat connectivity is a central factor in shaping aquatic biological communities, but few tools exist to describe and quantify
this attribute at a network scale in riverine systems. Here, we develop a new index to quantify longitudinal connectivity
of river networks based on the expected probability of an organism being able to move freely between two random points of
the network. We apply this index to two fish life histories and evaluate the effects of the number, passability, and placement
of barriers on river network connectivity through the use of simulated dendritic ecological networks. We then extend the index
to a real world dendritic river system in Newfoundland, Canada. Our results indicate that connectivity in river systems, as
represented by our index, is most impacted by the first few barriers added to the system. This is in contrast to terrestrial
systems, which are more resilient to low levels of connectivity. The results show a curvilinear relationship between barrier
passability and structural connectivity. This suggests that an incremental improvement in passability would result in a greater
improvement to river network connectivity for more permeable barriers than for less permeable barriers. Our analysis of the
index in simulated and real river networks also showed that barrier placement played an important role in connectivity. Not
surprisingly, barriers located near the river mouth have the greatest impact on fish with diadromous life histories while
those located near the center of the river network have the most impact on fish with potadromous life histories. The proposed
index is conceptually simple and sufficiently flexible to deal with variations in river structure and biological communities.
The index will enable researchers to account for connectivity in habitat studies and will also allow resource managers to
characterize watersheds, assess cumulative impacts of multiple barriers and determine priorities for restoration. 相似文献
54.
F M Cocker T J Newby R M Gaskell P A Evans C J Gaskell C R Stokes D A Harbour J F Bourne 《Research in veterinary science》1986,41(3):323-330
Intranasal vaccination with a cold-adapted strain of feline herpesvirus type 1 (FHV-1) two days before challenge gave partial protection, and four days before challenge gave complete protection, against feline viral rhinotracheitis. Protection at this time appeared to be specific since vaccination with FHV-1 did not affect the disease caused by the unrelated feline calicivirus. The time course of onset of protection also confirmed that the protective mechanism was likely to be specific. However, six days after vaccination only low levels of FHV-specific IgA and IgM antibody and of interferon were found in serum and nasal washings. In lymphocyte transformation assays neither peripheral blood lymphocytes nor tonsil lymphocytes gave a significant proliferative response in the presence of FHV antigen. Pathogenesis experiments demonstrated that the tonsil and nasal turbinates were the most important sites of virulent FHV-1 replication. Vaccination significantly reduced levels of infectious virus found in both sites. The results provide evidence that no one mechanism is responsible for protection following vaccination but local specific responses are more likely to be involved. 相似文献
55.
Five-day-old pigs challenged with 10(3) pathogenic Escherichia coli (nalidixic acid resistant) showed no clinical signs of disease until subsequently weaned at three weeks. Dietary manipulation was shown to influence xylose malabsorption, diarrhoea and bacterial proliferation after weaning. Brief, but not continuous, contact with the diet before weaning markedly increased the severity of subsequent disease after weaning. Immunogenicity of the weaning diet was critical for the development of the disease. Two diets, identical except that in one the protein source (casein) had previously been enzymatically hydrolysed, were compared. Pigs fed the predigested diet showed no clinical signs of post weaning diarrhoea whereas those fed the untreated casein all developed diarrhoea. 相似文献
56.
D R Jones T J Gruffydd-Jones C R Stokes F J Bourne 《American journal of veterinary research》1992,53(4):457-465
Detection of autoantibody, complement, or both bound to RBC is an essential requirement for unequivocal diagnosis of immune-mediated hemolytic anemia in dogs. An enzyme-linked antiglobulin test was adapted for laboratory diagnosis of this disease. The refinement and routine use of this assay have allowed further observation of the pathogenesis of the disease process. In particular, degree of hemolysis can be related to the degree of RBC sensitization associated with primary immune-mediated hemolytic anemia, and this correlation is highest for IgG autoantibody. Results indicate that autoantibody isotype might have an important role in the hemolytic process. 相似文献
57.
Concentrations of uterine luminal prostaglandins in mares with acute and persistent endometritis 总被引:1,自引:0,他引:1
Intrauterine infusion of 1 per cent oyster glycogen solution was used to induce acute endometritis in four genitally normal mares. Numbers of viable neutrophils recovered in uterine washings had increased by 1 h after infusion and remained elevated for at least 72 h. There was a significant correlation between numbers of viable neutrophils and total protein concentrations and between prostaglandin (PG)F and PGE2 concentrations in washings. There was also a significant relationship between concentrations of 15-keto-13, 14-dihydro PGF2 alpha in plasma and PGF in washings. Intrauterine concentrations of PGF were influenced by cycle stage and in turn the induced acute endometritis interfered with normal ovarian function. Mares with persistent endometritis had significantly higher concentrations of PGF and total protein and percentage of neutrophils and mononuclear cells in washings than normal mares. White blood cells from mares were capable of producing PGF and PGE2 in vitro. 相似文献
58.
Cellular and humoral defence mechanisms in mares susceptible and resistant to persistent endometritis 总被引:2,自引:0,他引:2
Both random and directional migration of blood neutrophils from 9 mares susceptible to persistent endometritis were significantly less (p less than 0.05) than neutrophils from 8 resistant mares. Serum from susceptible mares had significantly more (p less than 0.01) chemotactic activity than serum from resistant mares. Although phagocytosis of yeast blastospores by blood neutrophils from 4 resistant and 3 susceptible mares was similar, uterine neutrophils from susceptible mares were significantly worse (p less than 0.01) at phagocytosis than uterine neutrophils from resistant mares. Uterine washings from 17 susceptible mares were significantly better at opsonising yeast blastospores than washings from 14 resistant mares; however, washings from both groups had a similar ability to promote killing of S. zooepidemicus by neutrophils. When an immunologically non-specific endometritis was induced, washings from 3 susceptible mares were significantly worse at promoting bactericidal activity by 144 h than washings from 4 resistant mares (p less than 0.01). Haemolytic complement activity was significantly greater (p less than 0.001) in washings from 17 susceptible mares than from 14 resistant mares. Induction of acute endometritis resulted in high levels of haemolytic complement activity in 2 of 3 susceptible mares at 24 and 144 h, but only in small increases in 4 resistant mares. Thus, some abnormalities in neutrophil function were detected and a possible defect in promotion of neutrophil bactericidal activity by uterine secretions from susceptible mares but there was no evidence for any deficiency in haemolytic complement activity. 相似文献
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