全文获取类型
收费全文 | 156篇 |
免费 | 4篇 |
专业分类
林业 | 22篇 |
农学 | 3篇 |
基础科学 | 1篇 |
41篇 | |
综合类 | 30篇 |
农作物 | 13篇 |
水产渔业 | 21篇 |
畜牧兽医 | 16篇 |
园艺 | 5篇 |
植物保护 | 8篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 9篇 |
2020年 | 6篇 |
2019年 | 3篇 |
2018年 | 2篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 2篇 |
2014年 | 9篇 |
2013年 | 8篇 |
2012年 | 5篇 |
2011年 | 14篇 |
2010年 | 9篇 |
2009年 | 7篇 |
2008年 | 8篇 |
2007年 | 6篇 |
2006年 | 12篇 |
2005年 | 10篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 12篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1990年 | 2篇 |
1985年 | 1篇 |
1982年 | 1篇 |
1938年 | 1篇 |
1936年 | 1篇 |
排序方式: 共有160条查询结果,搜索用时 11 毫秒
61.
Variability in microsatellite DNA markers in gynogenetic and backcross progenies obtained from ornamental (koi) carp (Cyprinus carpio L.) × goldfish (Carassius auratus L.) hybrid females 下载免费PDF全文
Inheritance and segregation at five microsatellite loci were studied in diploid gynogenetic and triploid backcross progenies obtained from koi × goldfish hybrid females, which produce diploid eggs. Gynogenetic and backcross progenies were obtained from three individual hybrid females by inseminating eggs with genetically inactivated and intact sperm of parental species respectively; no shock treatments were applied to the early embryos. Complete absence of paternally specific alleles at all investigated microsatellite loci has proven successful genetic inactivation of spermatozoa by irradiation and confirmed gynogenetic origin of progenies. Genotypic segregations at microsatellite loci showed almost complete homogeneity of gynogenetic progenies and their identity to female parents. These results correspond with previous cytogenetic data on the occurrence of premeiotic endomitosis in hybrid females producing diploid eggs. Fish from triploid backcross progenies had genotypes resulting from combination of entire diploid female genome and haploid genome from male. 相似文献
62.
Serena Ciarroni Christopher R. Clarke Haijie Liu Noam Eckshtain-Levi Angelo Mazzaglia Giorgio M. Balestra Boris A. Vinatzer 《Journal of General Plant Pathology》2018,84(3):169-175
Plants and animals independently evolved the ability to recognize flagellin (also called FliC), the building block of the bacterial flagellum, as part of their innate immune response. While animals recognize a relatively large region of FliC, most plants recognize one or two short epitopes of FliC: flg22 and flgII-28. However, since most research in plants has focused on flg22 and flgII-28 and not the actual FliC protein, the importance of any FliC region beyond the two epitopes in plant immunity is poorly understood. Here we report cloning, overexpression, and purification of a Pseudomonas syringae FliC fragment from amino acid 1 to 143, which includes both FliC epitopes and the adjacent alpha helices. Exposing Arabidopsis thaliana leaves to FliC1–143 did not reveal any additional FliC recognition capabilities beyond flg22. However, while the kiwifruit species Actinidia arguta did not respond to either flg22 or flgII-28, treatment of A. arguta leaves with FliC1–143 triggered a significant reactive oxygen response, indicating recognition. This result suggests that in some plant species, recognition of FliC requires regions of FliC beyond the two well-known epitopes and that FliC1–143 represents a useful tool in the study of plant immunity. 相似文献
63.
Svetoch EA Eruslanov BV Perelygin VV Mitsevich EV Mitsevich IP Borzenkov VN Levchuk VP Svetoch OE Kovalev YN Stepanshin YG Siragusa GR Seal BS Stern NJ 《Journal of agricultural and food chemistry》2008,56(6):1942-1948
An effective bacteriocin was identified and characterized. Lactic acid bacteria were screened against Campylobacter jejuni. One bacteriocin producer, Enterococcus faecium (NRRL B-30746), was studied. The isolate was grown, and the bacteriocin was purified to single-band homogeneity. Biochemical traits indicated that the peptide was a Class IIa bacteriocin, and it was named E 50-52. The bacteriocin had a molecular weight of 3339.7 and an isoelectric point of 8.0. The minimal inhibitory concentrations of E 50-52 against C. jejuni, Yersinia spp., Salmonella spp., Escherichia coli O157:H7, Shigella dysenteriae, Morganella morganii, Staphylococcus spp., and Listeria spp. ranged from 0.025 to 32 microg/mL. In therapeutic broiler trials, oral treatment with E 50-52 reduced both C. jejuni and Salmonella enteritidis by more than 100,000-fold in the ceca, and systemic S. enteritidis was reduced in the liver and spleen. The wide range of antibacterial activity of bacteriocin E 50-52 against pathogens provides a promising alternative to antibiotics. 相似文献
64.
An experiment was conducted at Mizoram University, Aizawl, India during 2015-2017 to study the effect of integrated nutrient management on yield and quality of lemon cv. ‘Assam lemon’ (Citrus limon Burm.). The experiment was comprised of nine treatments viz. T1: 100% recommended dose of fertilizers (RDF) through inorganic fertilizers (IF); T2: 100% N through Farm Yard Manure (FYM); T3: 75% N through FYM + 25% through IF; T4: 50% N through FYM + 50% through IF; T5:75% N through FYM +25% through IF + Azotobacter + phosphate solubilizing bacteria (PSB)+ potash solubilizing bacteria (KSB); T6: 50% N through FYM + 50% through IF+ Azotobacter + PSB?+?KSB; T7: 75% N through FYM + 25% through IF?+?Azospirillum + AMF?+?KSB; T8: 50% N through FYM + 50% through IF+ Azospirillum + arbuscular mychorrhizal fungi (AMF) + KSB; T9: control. Among all treatments, T5 recorded highest value with respect to growth, yield and soil quality. While, T7 showed superiority in quality characters. 相似文献
65.
Mechanisms regulating inter‐annual variability in zooplankton advection over the Lofoten shelf,implications for cod larvae survival 下载免费PDF全文
Boris Espinasse Vigdis Tverberg Sünnje L. Basedow Tore Hattermann Ole Anders Nøst Jon Albretsen Jofrid SkarĐhamar Ketil Eiane 《Fisheries Oceanography》2017,26(3):299-315
Variability in the availability of suitable prey (mainly nauplii stages of Calanus spp.) in the early life stages of cod is likely a significant contributor to the fluctuations in the year‐class strength in the Arcto Norwegian cod stock. We have investigated the origin of the Lofoten shelf population of Calanus using a particle tracking model approach based on velocity fields simulated from 2002 to 2012. By performing backwards simulations of trajectories from particles released on the Lofoten shelf in early spring, we assessed the relative contribution of potential Calanus sources, such as the Lofoten Basin, the local overwintering on the continental shelf and the adjacent fjords. This analysis revealed significant interannual differences in advection patterns mainly driven by changes in wind conditions and variable strength of the Norwegian Coastal Current. Most of the particles advected into our study area originated on the shelf, and contrary to expectations, only a few originated from the Lofoten Basin where Calanus abundances tend to be high. These results suggest that contribution from the Lofoten Basin to the early spring shelf Calanus population may be overestimated and that contribution from more local sources, especially from adjacent fjords, may play a more important role than what has been previously anticipated. Our results highlight the necessity to improve quantification of shelf‐fjord exchange processes, as these may contribute more to the regulation of spring zooplankton stocks on the northern Norwegian shelf, and, as a result, may affect the survival of cod larvae in this area. 相似文献
66.
Hogenkamp A Isohadouten N Reemers SS Romijn RA Hemrika W White MR Tefsen B Vervelde L van Eijk M Veldhuizen EJ Haagsman HP 《Veterinary microbiology》2008,130(1-2):37-46
Many proteins of the calcium-dependent (C-type) lectin family have been shown to play an important role in innate immunity. They can bind to a broad range of carbohydrates, which enables them to interact with ligands present on the surface of micro-organisms. We previously reported the finding of a new putative chicken lectin, which was predominantly localized to the respiratory tract, and thus termed chicken lung lectin (cLL). In order to investigate the biochemical and biophysical properties of cLL, the recombinant protein was expressed, affinity purified and characterized. Recombinant cLL was expressed as four differently sized peptides, which is most likely due to post-translational modification. Crosslinking of the protein led to the formation of two high-molecular weight products, indicating that cLL forms trimeric and possibly even multimeric subunits. cLL was shown to have lectin activity, preferentially binding to alpha-mannose in a calcium-dependent manner. Furthermore, cLL was shown to inhibit the haemagglutination-activity of human isolates of influenza A virus, subtype H3N2 and H1N1. These result show that cLL is a true C-type lectin with a very distinct sugar specificity, and that this chicken lectin could play an important role in innate immunity. 相似文献
67.
Accumulation of coal humic acids by wheat seedlings: Direct evidence using tritium autoradiography and occurrence in lipid fraction 下载免费PDF全文
Natalia A. Kulikova Gennadii A. Badun Viktor I. Korobkov Maria G. Chernysheva Eugenia A. Tsvetkova Dmitry P. Abroskin Andrey I. Konstantinov Boris T. Zaitchik Alexander O. Ruzhitsky Irina V. Perminova 《植物养料与土壤学杂志》2014,177(6):875-883
Humic acids (HA) are natural organic compounds that are important components of organic matter. The accumulation, distribution, and fate of tritium‐labeled HA prepared from coal were analyzed using wheat (Triticum aestivum L.) seedlings. There was a period of rapid accumulation of HA followed by a slower one in the period from 1 to 24 h. There was a significant decrease in HA accumulation at low temperature, indicating that the slower rate of HA accumulation represented a membrane‐mediated process. HA distribution in plant tissues was analyzed using autoradiography. In all cases, HA concentration was considerably higher in the roots than in the shoots. Detailed examination of autoradiograms showed that there was preferential accumulation of HA in the apices of roots and shoots of wheat seedlings. Lipid fractions were extracted from seedlings and analyzed with thin layer chromatography and gas chromatography–mass spectrometry. These analyses revealed that labeled HA were present in the neutral lipid fraction consisting mainly of alkanes and alkenes, which are usually found in plant waxes, associated with the cuticle and suberized tissues. Based on these data, it is suggested that HA‐derived hydrocarbons may be used in wax biosynthesis. This role could explain the mitigating activity of humic substances under stress conditions. 相似文献
68.
Albert Rovira Rhoda Ballinger Carles Ibáñez Paul Parker M. Dolores Dominguez Xavier Simon Andrzej Lewandowski Boris Hochfeld Marian Tudor Liesbeth Vernaeve 《Journal of Soils and Sediments》2014,14(8):1493-1512
Purpose
We analysed the status of current water and sediment management practices in six deltas and estuaries, which were part of the European DELTANET, INTERREG-funded network.Materials and methods
These systems—the Danube, Ebro and Vistula deltas and the Elbe, Minho and Severn estuaries—represent different geographic regions of Europe. This enables comparison between the sites’ approaches to common coastal issues, notably those associated with sediment budgets, contamination and flood risk. Based on documentary analysis, workshop events and expert discussion, we employ a simple classification scheme to distinguish between levels of risk from these aspects.Results
We suggest that flood risk is the most significant risk, followed by upstream sediment retention and sediment aggradation. Chemical contamination, though less severe, is not unimportant. Key management issues include a lack of environmental quality standards for sediment and suspended particulate matter, as well as the limited deployment of monitoring programmes, regular sediment sampling and associated chemical analyses.Conclusions
These include both general and specific recommendations. Within these, the limited scope of integrated plans that aim for sustainability of the respective systems is highlighted. It is suggested that these do not challenge traditional, classical engineering approaches sufficiently. Nor do they address the origin of many environmental problems, especially those which are closely linked to short-term political and economic priorities. 相似文献69.
70.
Landi MT Bauer J Pfeiffer RM Elder DE Hulley B Minghetti P Calista D Kanetsky PA Pinkel D Bastian BC 《Science (New York, N.Y.)》2006,313(5786):521-522
Germline variants in MC1R, the gene encoding the melanocortin-1 receptor, and sun exposure increase risk for melanoma in Caucasians. The majority of melanomas that occur on skin with little evidence of chronic sun-induced damage (non-CSD melanoma) have mutations in the BRAF oncogene, whereas in melanomas on skin with marked CSD (CSD melanoma) these mutations are less frequent. In two independent Caucasian populations, we show that MC1R variants are strongly associated with BRAF mutations in non-CSD melanomas. In this tumor subtype, the risk for melanoma associated with MC1R is due to an increase in risk of developing melanomas with BRAF mutations. 相似文献