Determination of reference intervals for metabolic profile of Hanwoo cows at early,middle and late gestation periods

Background: Metabolic profile was initially designed as a presymptomatic diagnostic aid based on statistical analyses of blood metabolites to provide an early warning of certain types of metabolic disorder. However, there is little metabolic profile data available about Korean Hanwoo cows. Therefore, this study aimed to determine the reference intervals of metabolic profile for Korean Hanwoo cows.Methods: Healthy animals(2,205) were selected and divided into early(day 1 to 95), middle(day 96 to 190) and late(day 191 to 285) period according to their gestating period. Metabolic profile including total protein(TP), albumin(Alb), urea(UREA), glucose(Glu), total cholesterol(T-Cho), long-chain fatty acid(LCFA), aspartate aminotransferase(AST),gamma-glutamyl transpeptidase(GGT), creatinine(Crea), calcium(Ca), inorganic phosphorous(i P) and magnesium(Mg)were analyzed using a TBA-40 FR automatic biochemical analyzer. The data of Korean Hanwoo cows were then compared to those of the Japanese Wagyu cows.Results: Most of the data of the Korean Hanwoo cows were relatively higher than those of Japanese Wagyu cows, with the exception of Glu and GGT. This may indicate that the nutritional level of feed for the Korean Hanwoo cows was higher than that of the Japanese Wagyu cows because of the different feeding system. In particular, relatively higher levels of UREA and LCFA were observed in the Korean Hanwoo cows, and this may also contribute to the low reproduction efficiency.Conclusions: These findings may provide some theoretical basis for understanding the reproductive and feeding situation of Korean Hanwoo cows.     

蛋氨酸螯合锌不同添加量对肉用仔鸡生产性能的影响

将600只鸡分成3组，每组200只鸡。分别为对照组、试验1组和试验2组。对照组微量元素采用的饲料级无机盐；试验1组和试验2组分别添加60mg/kg，40mg/kg的羟基蛋氨酸螯合锌，以研究蛋氨酸螯合锌不同添加量对肉用仔鸡生产性能影响。试验结果表明，以微量元素氨基酸螯合物代替无机盐可显著提高内用仔鸡的生长速度和饲料转化率，提高养殖的经济效益,且肉用仟鸡日粮中蛋氨酸螯舍锌添加量以每千克饲料中添加60mg的效果最好。     

中国施用生物炭对番茄产量和品质效应的Meta分析

为定量分析生物炭施用对番茄产量和品质的时空效应和影响机制,同时为番茄生产中生物炭合理施用量的确定提供依据。该研究通过检索文献整合已发表的相关田间试验数据,采用Meta-analysis法分析了生物炭施用条件下,生物炭特性、土壤条件、田间管理措施与时空特性对番茄产量和品质的综合效应,并对各影响因素进行通径分析。结果表明：施用生物炭显著增加了番茄产量、果实Vc、可溶性糖和可溶性固形物含量（P<0.05）,增长率分别为 30.7%（95%置信区间（confidence interval,CI） 为27.6%～33.9%）、13.8%（95% CI为10.8%～16.9%）、6.7%（95% CI为3.3%～10.2%）和10.7%（95% CI为5.8%～15.8%）,而番茄果实硝酸盐含量降低了3.1%（95% CI为-6.7%～0.8%）,对番茄果实糖酸比含量增加效应不显著（95% CI为-3.0%～7.9%）;番茄产量效应的亚组分析表明,生物炭施用在不同时间和区域特征下对番茄增产效应影响显著（P＜0.01）,随着时间的推移,生物炭施用对番茄增产效应呈波动式增长趋势,华北、东北农田区的番茄增产效应不显著;土壤有机质含量与番茄增产率呈正相关关系,土壤pH值与番茄增产率呈负相关关系,番茄增产率随土壤全氮及硝酸钾含量的增加呈先增后减趋势;田间管理措施对番茄产量效应分析结果表明,生物炭施加量>40～80 t/hm²时,增产率可达37.8%（95% CI为33.5%～42.1%）,生物炭热解温度在400～600 ℃内,生物炭pH值控制在>8～9为最优;生物炭施用量、生物炭pH值和产量年份是影响生物炭增产效果的3个主要因素。研究结果可为番茄生产中科学、合理生物炭施用量的确定提供理论支撑。     

海带岩藻聚糖及其级分在氧化应激条件下对肉鸡巨噬细胞免疫功能的影响

【目的】在氧化应激条件下,研究海带岩藻聚糖及其级分对肉鸡巨噬细胞免疫功能的影响,初步探讨海带岩藻聚糖及其级分的免疫调节功能与抗氧化作用的关系。【方法】以叔丁基过氧化氢（tBOOH）为活性氧诱导剂,加入肉鸡腹腔巨噬细胞培养液中,测定细胞培养液中抗氧化酶的变化,巨噬细胞分泌一氧化氮（NO）、诱导型一氧化氮合成酶（iNOS）、以及细胞因子IL-1和TFN-α等免疫指标的变化。【结果】用tBOOH处理后的巨噬细胞,细胞存活率显著下降（P＜0.05）,乳酸脱氢酶（LDH）和丙二醛（MDA）显著升高（P＜0.05）,SOD和GSH-Px酶活性显著降低（P＜0.05）。巨噬细胞的吞噬活性显著降低（P＜0.05）,NO、iNOS和呼吸爆发显著降低（P＜0.05）,细胞因子IL-1分泌量显著下降（P＜0.05）,表明细胞出现明显的氧化损伤。在培养液中加入海带岩藻聚糖及其级分,能够缓解细胞的氧化应激,使得细胞存活率上升,LDH和MDA降低,SOD和GSH-Px升高,免疫指标均有不同程度的恢复。【结论】海带岩藻聚糖及其级分具有抗氧化活性,不同级分的抗氧化活性存在差异,其中级分Ⅲ、级分Ⅳ的作用较强。海带岩藻聚糖对巨噬细胞的免疫促进作用与其抗氧化活性有关。     

氨气对肉鸡健康的影响及应对措施

氨气是鸡舍内危害最大的气体,长期氨气应激不利于肉鸡的健康生长。加强对肉鸡舍氨气浓度的监测,研究氨气对肉鸡健康的影响以及减排措施对指导鸡舍氨气浓度的合理调控和健康养殖的发展具有重要意义。本文主要通过综述舍内氨气的来源,氨气对肉鸡生长性能、屠宰性能和肉品质、免疫功能、呼吸道、繁殖性能、福利等方面的影响以及舍内氨气的监测和减排措施,为深入研究氨气对肉鸡健康影响的作用机制以及合理调控肉鸡舍内氨气浓度提供参考。     

Cefquinome Concentrations in Endometrium after Intrauterine Treatment of Cobactan 4.5%® in Mares and Inflammatory Response of the Endometrium to this Treatment

This study was conducted to measure the concentration of cefquinome in the endometrium of mares after intrauterine treatment and to evaluate associated inflammation. Mares (n = 14) were randomly assigned to one of the following groups: (i) control (n = 4) were either not treated (n = 2) or received (n = 2) lactated Ringer's intrauterine for 1 or 3 days; (ii) treated mares (n = 10) received intrauterine cefquinome for 1 or 3 days. After at least 10 days had passed following the last treatment and ovulation, mares were given Prostaglandin F2α (PGF2α) and were randomly assigned to an alternate treatment. Endometrial biopsy samples were taken at 2, 8, 24 and 48 h, or at 4, 12 and 36 h, after the last treatment. Biopsy samples were taken at the same time points from control mares (n = 2) and lactated Ringer-treated mares (n = 2). Cefquinome concentrations were quantified using a high-performance liquid chromatography (HPLC) assay and inflammation was assessed using haematoxylin and eosin (H&E)-stained sections. Concentrations of cefquinome [559 (1 day) and 595 μg/g (3 days) at 2 h, and 403 (1 day) and 370 μg/g (3 days) at 4 h] were similar between treatment groups at 2 and 4 h after treatment (p > 0.05). At 8 h, as well as at 24 and 48 h, concentrations were greater in the 3-day group (17 vs 301 μg/g, 3 vs 80 μg/g and 0.1 vs 0.2 μg/g, respectively) (p < 0.05). No significant differences (p > 0.05) in the inflammatory response at 2–48 h after treatment were found between groups.