蓖麻毒蛋白A链基因克隆与重复表达载体构建

利用降落PCR的方法,从蓖麻基因组DNA中克隆毒蛋白A链基因的560bp片段;利用一步法将该基因的2个正义片段和2个反义片段分别与pBI-121质粒连接,构建含该基因的正义重复和反义重复表达载体,为利用共抑制技术抑制蓖麻毒蛋白A链基因表达的研究奠定基础。     

玉米秸秆还田对盐碱地土壤真菌多样性的影响

为揭示盐碱地秸秆还田改良中土壤真菌群落结构及其多样性变化,以未开垦盐碱地(ZH1)、非盐碱连作玉米田(ZH2)和秸秆还田盐碱玉米田(ZH3)为材料,在玉米吐丝期,利用高通量测序技术与相关生物信息学分析结合,研究表层土壤真菌群落丰富度、多样性和群落结构的变化。结果表明,3个处理土壤真菌群落结构和丰度差异大,已知菌属中ZH1的优势属为马拉色菌,ZH2优势属为暗球腔菌属,ZH3优势属为链格孢属。玉米秸秆还田对盐碱地土壤真菌多样性的影响较大,两年玉米秸秆还田盐碱土壤真菌多样性和物种丰度与未开垦盐碱地比较均有所下降。     

SA对水分胁迫下红小豆叶片过氧化物酶活性的影响

水杨酸(SA)处理红小豆叶片3d后,处理叶片及未接受处理叶片内的POD活性增加,并且不同浓度的SA对叶片内POD活性影响不同.通过测定渗透胁迫14d的红小豆植株叶片发现,SA处理能显著增加渗透胁迫下叶片中的POD活性.     

Progress on role of exosomes derived from leukemia cells

Exosomes are bilayer-lipid membrane nanovesicle from almost all living cell types which are involved in intercellular substance transporting and signaling communication. Exosomes are 30~120 nm in diameter, can transfer bioactive molecules including DNA, RNA, microRNA, protein as well as lipids derived from parents' cells to recipient cells by body fluids, and specifically influence their physiological or pathological conditions. Leukemia is due to malignant proliferation of hematopoietic stem and progenitor cells. It was reported that leukemia cells derived exosomes play a key role in disease progression, drug resistance, and predict prognosis. This paper will outline the role of exosomes derived from leukemia cells and provide important information to help explore the molecular pathogenesis, biomarker as well as therapeutic target of leukemia.     

姬松茸子实体中活性多肽提取工艺初探

通过单因素考察及正交实验法对姬松茸子实体活性肽提取条件进行了优化。结果显示在姬松茸干子实体粉中加入8倍体积的水,在温度为4℃和pH9．0的条件下,用70％乙醇的沉淀提取6小时,得到提取液的活性肽的含量及抗氧化性均最优,经毛细管电泳图谱分析其活性肽分布也较适宜。     

Study on Environmental Quality Parameters in Broiler House with Three-overlap Cages in Winter and Autumn

The study was conducted to detect the indoor environmental quality parameters of broiler house with three-overlap cages and analyze the differences in winter and autumn. In the process of feeding, the same farming house in the same farm was chosen to carry out the test. The indoor ambient temperature, humidity and the air quality were determined in chicken house in different seasons. The measure points were distributed in 4 different positions in the house. The experiment period was from 3 to 6 weeks of broilers.The results showed that there was no significant difference in the ambient temperature between autumn and winter during experimental period (P >0.05). The ambient temperature in the house in winter was slightly higher than in autumn. The humidity in autumn was significantly higher than that in winter (P <0.05).The temperature and humidity in two seasons could meet the requirements of broiler growth. There was no significant difference in ammonia concentration between autumn and winter, while ammonia level in winter was slightly higher than that in autumn. The carbon dioxide in the house was significantly higher in winter than that in autumn (P <0.05). The total number of airborne bacteria in winter was significantly higher than that in autumn (P <0.05). The indoor temperature kept constant in autumn and winter seasons in closed poultry house. In winter, the humidity was reduced, the carbon dioxide concentration was increased, and the total number of bacteria in the air was increased.So, the ventilation should increase at the same time of heating in winter.     

Effect of Humidity on Broiler Health and Corresponding Measures

With the development of intensive broiler raising, the influence of environmental factors on broiler health are increasingly prominent. Relative humidity is one of the most important indicators of broiler house environment.But in the production process of broiler farming,it is often neglected on the management of humidity in house. Neither low humidity nor high humidity is conducive to the healthy growth of broilers. It is of great significance to strengthen the monitoring of the humidity in broiler houses and study the effects of relative humidity on the health of broiler for guiding the rational control of the humidity in broiler houses and the development of healthy breeding. In this paper, we summarized and analyzed the effects of relative humidity on thermal regulating,growth performance, reproductive performance, blood parameters, meat quality and respiratory tract of broilers, and the monitor and control measures of humidity in the poultry house, aimed to provide a reference for further research on the mechanism of relative humidity affects broiler health and rational regulation of humidity in broiler house.     

宁夏荒漠草原蒙古冰草资源分配特征

为了解不同群落生境蒙古冰草(Agropyron mongolicum)资源权衡策略及种群维持机制,在宁夏荒漠草原选取以蒙古冰草为优势种或亚优势种的群落为研究对象,研究了不同群落蒙古冰草资源分配特征。结果表明：(1)蒙古冰草个体生物量及根冠比在不同群落之间差异显著(P<0.05),构件生物量分配均表现为根>茎>叶>穗;(2)不同群落蒙古冰草各构件C,N,P含量均以根部最低,C/N,C/P以茎部最高,N/P以叶部为高,比值范围为18～23;不同群落之间则表现为C/N和C/P总体以M2群落较高,地上部各构件N/P以M3群落较高(P<0.05);(3)蒙古冰草地下生物量与土壤含水量呈显著负相关,与各构件N,P元素含量呈显著正相关;各构件P元素含量与其生物量分配比及地上地下生物量之间存在显著相关关系(P<0.05)。综上,蒙古冰草可通过调整自身构件分配格局和养分利用策略保证其在不同群落生境的生长发育和繁殖。