Non-penetrative percussion stunning of sheep and calves

Attempts were made to stun lambs, adult sheep and calves by a non-penetrative percussive method. Using an adapted Cash pistol, with a concave padded impact head, impulses were produced which effectively stunned 96% of lambs but induced brain haemorrhages in up to 7% of the animals. The signs and duration of an effective percussive stun were observed and recorded. Calves were readily stunned by percussive methods but brain haemorrhages frequently occurred. Impulses sufficiently large to stun adult sheep, with a non-penetrating impact head, were produced from an adapted Hantover pneumatic cattle stunner. Comparative trials, using electrical and percussive stunners, demonstrated a significant decrease in the prevalence of blood splash in lambs stunned by percussion.     

几个牛肉品质性状的QTL分析

测定了利木赞×娟姗牛杂交一代3头公牛所有回交后代的背最长肌和半腱肌的剪切力、pH值及煮 制损失3个表型性状,与已构建的遗传连锁图谱和基因型值相结合,利用QTL Express软件的“Half-Sib QTL Analysis Servlet”进行QTL分析。共发现6个可能的QTL位点,其中背最长肌剪切力性状3个QTL(BTA2 45 cM,BTA29 54 cM)、半腱肌剪切力1个QTL(BTA2 12 cM)、半腱肌pH值1个QTL(BTA24 0 cM)、背最长肌 煮制损失1个QTL(BTA142 cM)。     

Radar detection of the nucleus and coma of comet hyakutake

Radar observations of comet Hyakutake (C/1996 B2) made at the Goldstone Deep Space Communications Complex in California have detected echoes from the nucleus and from large grains in the inner coma. The nucleus of this bright comet was estimated to be only 2 to 3 kilometers in diameter. Models of the coma echo indicate backscatter from porous, centimeter-size grains ejected anisotropically at velocities of tens of meters per second. The radar observations suggest that a comet's activity may be a poor indicator of its size and provide evidence that large grains constitute an important component of the mass loss from a typical active comet.     

Seed Mapping of Sugar Beet

Individual plant care may well become embodied in precision farming in the future and will lead to new opportunities in agricultural crop management. The objective of this project was to develop and evaluate a data logging system attached to a precision seeder to enable high accuracy seed position mapping of a field of sugar beet. A Real Time Kinematic Global Positioning System (RTK GPS), optical seed detectors and a data logging system were retrofitted on to a precision seeder to map the seeds as they were planted. The average error between the seed map and the actual plant map was about 16–43 mm depending on vehicle speed and seed spacing. The results showed that the overall accuracy of the estimated plant positions was acceptable for the guidance of vehicles and implements as well as potential individual plant treatments.     

Developing Strategies for Spatially Variable Nitrogen Application in Cereals, Part I: Winter Barley

For precision agriculture to provide both economic and environmental benefits over conventional farm practice, management strategies must be developed to accommodate the spatial variability in crop performance that occurs within fields. Experiments were established in crops of winter barley (Hordeum vulgare L.) over three seasons. The aim of which was to evaluate a set of variable rate nitrogen strategies and examining the spatial variation in crop response to applied N. The optimum N application rate varied from 90 to in excess of 160 kg [N] ha⁻¹ in different parts of the field, which supports the case for applying spatially variable rates of N. This, however, is highly dependent on seasonal variations, e.g. the quantity and distribution of rainfall and the effect that this has on soil moisture deficits and crop growth. Estimates of yield potential, produced from either historic yield data or shoot density maps derived from airborne digital photographic images, were used to divide experimental strips into management zones. These zones were then managed according to two N application strategies. The results from the historic yield approach, based on 3 yr of yield data, were inconsistent, and it was concluded that that this approach, which is currently the most practical commercial system, does not provide a suitable basis for varying N rates. The shoot density approach, however, offered considerably greater potential as it takes account of variation in the current crop. Using this approach, it was found that applying additional N to areas with a low shoot population and reducing N to areas with a high shoot population resulted in an average strategy benefit of up to 0·36 t ha⁻¹ compared with standard farm practice.     

Developing Strategies for Spatially Variable Nitrogen Application in Cereals, Part II: Wheat

For precision agriculture to provide both economic and environmental benefits over conventional farm practice, management strategies must be developed to accommodate the spatial variability in crop performance that occurs within fields. Experiments were established in crops of wheat (Triticum aestivum) over three seasons in two fields. The aim was to evaluate a set of variable rate nitrogen strategies and examining the spatial variation in crop response to applied N. The optimum N application rate in the field with three different soil series (predominantly calcareous silty clay loam over oolitic limestone) was uniform across the field. In contrast the other field with uniform soil type (slightly calcareous brown clay loam), provided a more variable response. Estimates of yield potential, produced from either historic yield data or shoot density maps derived from airborne digital photographic images, were used to divide experimental strips into management zones. These zones were then managed according to two N application strategies. The results from the historic yield approach, which is currently the most practical commercial system, based on 3 yr of yield data, were variable with no overall yield or economic advantages. It was concluded that that this approach may not provide a suitable basis for varying N rates. The shoot density approach, however, offered considerably greater potential as it takes account of variation in the current crop. Using this approach, it was found that there was insufficient variation in the shoot density in the field with diverse soil types. However, in the field with the uniform soil type, applying additional N to areas with a low shoot population and maintaining the standard N rate to areas with an average shoot population resulted in an average strategy benefit of up to 0·46 t ha⁻¹ compared with standard farm practice. It is necessary to combine the ‘real-time’ data on relative crop structure, obtained by remote sensing with ground truth assessments and absolute benchmark values to successfully adjust N input levels to maximise yield.     

The Analysis of Spatial and Temporal Trends in Yield Map Data over Six Years

A quantitative analysis of yield data from four fields over 6 years was carried out to identify the spatial and temporal trends. The methodology was modified from previous work to separate the temporal effects into two parts; the inter-year offset and the temporal variance. The inter-year offset quantifies the overall differences in yield between 1 year and the next, whereas the temporal variance indicates the amount of change at a particular point over time. Results from these fields show that the significant spatial variability found within each individual yield map cancelled out over time, leaving a relatively homogenous spatial trend map. The implications of these findings are that each field should be managed according to the current years’ conditions.     

Molecular expression and identification of caprine estrogen receptor gene 1 for fertility status in bucks

Estrogen and its receptors are essential for sexual development and reproduction. Oestrogen receptor alpha (ERα) is a nuclear receptor activated by the hormone oestrogen. In male, ERα is encoded by the gene ESR1 (oestrogen receptor1) responsible for better fertility. ESR1 is involved in the reabsorption of luminal fluid during the transit of spermatozoa from the testis to the head of the epididymis which is important for their survival and maturation during epididymal storage. The absence of ESR1 leads to reduced epididymal sperm content, reduced sperm motility and fertilizing ability. The present study was undertaken to investigate the expression and presence of ESR1 gene in fertile and low-fertile male goat breeds. We identified ESR1 gene through various molecular tools. Genotyping was carried out by high resonance melting analysis using Roche Light Cycler 480(LC-480) system and found three different genotypes. Genotypic frequency-AA (blue-0.67), BB(Red-0.2), AB(Green-0.08) with allele frequency A(0.71 and B (0.29). The predominance of this gene in head of epididymis in fertile bucks was confirmed by SDS-PAGE, Western blotting and immunohistochemistry. From the results, we corroborated that the present study provides a useful and effective way to predict male fertility in goat breeds, which in turn increases the percentage of fertility in flock leading to more number of offspring in a kidding season.     

Haptoglobin in the serum of thoroughbreds in training

A method is described for the measurement of haptoglobin in equine serum using the peroxidase activity of the haemoglobin-haptoglobin complex. The problems of interference with Fe2+ and Fe3+ ions are described. Normal values for haptoglobin in 629 blood samples from thoroughbreds in training are presented showing a log normal distribution with a 5 per cent to 95 per cent range of 0.42 to 1.7 g/litre. There was no consistent alteration in haptoglobin concentration throughout the season in spite of a change in red cell size and total bilirubin concentration. It is concluded that the measurement of haptoglobin is useful in the detection of frank haemolytic states but is insufficiently sensitive to detect mild red cell destruction. The test, after further investigation, might be used as an adjunct to the current batch of blood tests employed to assess liver function.     

Selenium and gamma-glutamyl transferase activity in the serum of thoroughbreds.

Selenium and gamma-glutamyl transferase activity has been measured in the serum of clinically health thoroughbreds. The thoroughbreds, whose performance was reported to be unsatisfactory, had consistently low concentrations of selenium and high activity of gamma-glutamyl transferase in the serum when compared with those whose performance was as expected. Vitamin E levels in the serum showed no such difference. The only other biochemical and haematological abnormality was lower serum phosphate concentrations in the unsatisfactory group. These results suggest that low concentrations of selenium in the serum may be associated with subclinical hepatic insufficiency in the thoroughbred.