Antidiarrhoeal activity of Strychnos potatorum seed extract in rats

The antidiarrhoeal activity of the methanol extract of the dried seeds of Strychnos potatorum (MESP) has been evaluated out in rats using different models (castor oil-induced diarrhoea, effects on gastrointestinal motility and on PGE(2)-induced gastric enteropooling. MESP (100, 200 and 400 mg/kg, p.o.) significantly (P<0.001) inhibited the frequency of defaecation and reduced the wetness of faecal droppings in castor oil-induced diarrhoea, decreased the propulsion of charcoal meal through the gastrointestinal tract, and also reduced the PGE(2)-induced enteropooling.     

孟加拉国丘陵地带桔园和灌木林地土壤物理性质的比较评价(英文)

对孟加拉国丘陵地带Mro部落经营的桔园与灌木林35%和55%两种坡地土壤物理性质进行了比较与评价。每一块用地内挖掘3个土壤剖面采集不同深度(0-5cm,5-15cm and 15-30cm)的土壤样品,测定土壤水分、有机质含量和土壤密度。最大持水量、田间持水量、干或湿土壤容重和土壤孔隙度的测定仅限于表层土壤。两种坡地桔园3个深度的土壤水分含量均显著高于灌木林地相同深度的土壤水分。桔园在55%坡地内土壤有机质含量低于林地含量值,而在35%坡地内的含量值则高于林地值。两块样地内上述两项测定值均在表层土壤中最高,且随土层深度的增加而降低。与灌木林地比较,桔园地表层土壤最大持水量、孔隙度和3个不同深度的土壤密度均较低。两块用地内不同坡地的表层土田间持水量有所差异,且桔园地干或湿表层土壤容重高于林地内的数值。表5参23。     

Yield and water use efficiency of cauliflower under varying irrigation frequencies and water application methods in Lower Gangetic Plain of India

A field study (1999-2000 to 2001-2002) was carried out to optimize the irrigation frequency and suitable water application methods for cauliflower with a view to increase curd yield (CY) and water use efficiency (WUE). Check Basin (CB), Each Furrow (EF) and Alternate Furrow (AF) methods were tested with three irrigation frequencies depending on the attainment of soil matric potential (Ψ_m) value at 0.2 m depth as: −0.03 MPa (F₁), −0.05 MPa (F₂) and −0.07 MPa (F₃). Maximum CY was recorded under F₁ and decreased by 10.4 and 31.4%, respectively under F₂ and F₃ frequencies. In contrast, WUE decreased by 9.3% from F₃ to F₁. Highest CY and WUE obtained under CB followed by EF and AF methods. Furrow application methods saved 12-24% irrigation water over CB method. Maximum soil water stress coefficient (K_s) recorded at curd development stage in comparison to other stages. Both seasonal evapotranspiration (ET_a) and yield-moisture stress index (Kys) recorded positive linear relationships with CY. Present study shows a crop response factor of 0.822 for cauliflower. In this region, cauliflower should be irrigated with check basin method at an interval of 8-10 days.     

Sulfur accumulation in Vertisols due to continuous gypsum application for six years and its effect on yield and biochemical constituents of soybean (Glycine Max L. Merrill)

Application of S through inorganic sources is recommended to the crops in the cropping system in S deficient soils of India. However, transformations of S in the soil profile following continuous gypsum application are not known. The accumulation of S into different fractions due to application of a total of 180—540 kg S ha^—1 over a six‐year‐period in soybean — wheat cropping system and its effect on the yield and quality of soybean produce were investigated in a vertisol area. About 25—38% of the S added was retained in the 90 cm depth of profile. Significant changes in the total S content in soil layers were recorded wherein a total of 360 kg S ha^—1 or more S was applied. Maximum increase in total S content was recorded in upper layers. Soluble S accumulated more in the 30—75 cm depth; whereas, proportion of sorbed fraction of S increased with depth. Build up of S in the surface layer of the profile was mostly related to the increase in organic fraction. The S accumulation in lower layers of the profile was mostly due to increase in soluble and sorbed fractions of S. Such an increase of the S content in the soil caused an increase in seed yield, uptake of S as well as content of oil and S containing amino acids, cystine, and methionine in seed of soybean crop.     

Influence of farmyard manure, inorganic fertilizers and weed control practices on some soil physical properties in a long-term experiment

Abstract. In a long-term experiment, soil physical properties were studied after 20 annual cycles of maize-wheat-fodder cowpea cropping with annual application of inorganic fertilizers and farmyard manure (FYM). Weeds were controlled by hand or by the use of herbicides. The hand weeding treatment resulted in a lowering of the bulk density of the surface layer (0-15 cm) and a significant increase in the subsurface (15–30 cm) density. Application of FYM significantly increased the soil organic carbon (OC), infiltration rate, water retention, aggregation and aggregate stability in water. Application of inorganic fertilizers had small but statistically significant effect in increasing soil OC.     

Influence of cell volume changes on protein synthesis in isolated hepatocytes of air-breathing walking catfish (<Emphasis Type="Italic">Clarias batrachus</Emphasis>)

The present study aimed at determining the effect of cell volume changes on protein synthesis, measured as the incorporation of [³H]leucine into acid-precipitable protein, in isolated hepatocytes of air-breathing walking catfish (Clarias batrachus). The rate of protein synthesis, which was recorded to be 10.02 ± 0.10 (n = 25) nmoles mg⁻¹ cell protein h⁻¹ in isotonic incubation conditions, increased/decreased significantly by 18 and 48%, respectively, following hypo- (−80 mOsmol l⁻¹)/hypertonic (+80 mOsmol l⁻¹) incubation conditions (adjusted with NaCl), with an accompanying increase/decrease of hepatic cell volume by 12 and 20%, respectively. Similar cell volume-sensitive changes of protein synthesis were also observed when the anisotonicity of incubation medium was adjusted with mannitol. Increase of hepatic cell volume by 9%, due to addition of glutamine plus glycine (5 mM each) to the isotonic control incubation medium, led to a significant increase of protein synthesis by 14%. Decrease of hepatic cell volume by 15 and 18%, due to addition of dibutyl-cAMP and adenosine in isotonic control incubation medium, led to a significant decrease of protein synthesis by 30 and 34%, respectively. Thus, it appears that the increase/decrease of hepatic cell volume, caused either by changing the extracellular osmolarity or by the presence of amino acids or certain other metabolites, leads to increase/decrease of protein synthesis, respectively, and shows a direct correction (r = 0.99) between the hepatic cell volume and protein synthesis in walking catfish. These cell volume-sensitive changes of protein synthesis probably help this walking catfish in fine tuning the different metabolic pathways for better adaptation during cell volume changes and also to avoid the adverse affects of osmotic stress. This is the first report of cell volume-sensitive changes of protein synthesis in hepatic cells of any teleosts.     

Feeding interval and photoperiod influence the growth performance of striped knifejaw,Oplegnathus fasciatus

A two‐factor experiment incorporating two photoperiods (16 L:8 D and 24 L:0 D) and four feeding intervals (6, 9, 12 and 24 h) was carried out to determine the most efficient feeding interval and photoperiod for striped knifejaw, Oplegnathus fasciatus. Fish were fed a commercial diet to apparent satiation for 10 weeks. Blood was collected to measure the stress indicators. Both the photoperiod and the feeding intervals had a significant effect on the growth performance of striped knifejaw; however, their interaction did not produce any significant effect. The final body weight, weight gain (%) and feed intake at 6‐, 9‐ and 12‐h feeding intervals under 16 L:8 D were significantly higher than those at the 24‐h interval irrespective of the photoperiod (P<0.05). The specific growth rate at 6, 9 and 12 h intervals of the 16 L:8 D photoperiod was significantly higher than that of the other feeding intervals, except at 12 h of the 24 L:0 D photoperiod (P<0.05). At the end of the experiment, there were no significant differences in the plasma levels of cortisol, glucose and total protein observed in fish exposed to different photoperiods compared with the initial levels. The results suggested that a combination of a 16 L:8 D photoperiod and either of 6‐, 9‐ or 12‐h intervals can be used to stimulate the growth performance in striped knifejaw.     

Effects of elevated CO2 and water stress on mineral concentration of cotton

Projected increases in atmospheric CO₂ concentrations may alter mineral and protein levels in plant tissues, systematically affecting growth, nutrient cycling and utilization, residue decomposition, and insect-plant interactions in the future. The free-air CO₂ enrichment (FACE) system provided an opportunity to monitor seasonal trends in nutrient status and crude protein content of cotton (Gossypium hirsutum L. cv. Deltapine 77) grown in a natural field setting without the limitations often imposed by growth chambers or reduced rooting volumes. In 1990, plants were exposed to two levels of atmospheric CO₂ (FACE, ≈ 550 μmol mol⁻¹ and CONTROL, ≈ 370 μmol mol⁻¹) and two irrigation regimes (100% and 75% replacement of evapotranspiration) beginning in early July. Cotton leaves, stem, and roots were sampled at different times during the season and analyzed for C, N, Ca, K, Mg, P, Cu, Fe, Mn, Zn, B, Mo, Si and protein. The N and protein concentrations of leaves, stems and roots were significantly lower in FACE plants than in CONTROL plants, but C:N ratios were higher for the FACE plants than the CONTROL plants. Some other elements were significantly affected by CO₂ enrichment, but not for all dates and all plant tissues. There were no significant effects in any of the data because of the irrigation treatment or the irrigation—CO₂ interaction. Reductions in tissue N and protein concentrations and the increases in the C:N with CO₂ enrichment have important implications for agricultural and natural systems and demand additional research.     

Sulfide Production by Sulfate Reducing Bacteria with Lactate as Feed in an Upflow Anaerobic Fixed Film Reactor

The sulfate reducing bacteria (SRB) have the capability of reducing sulfate (SO4-2) under anaerobic conditions into sulfide (S-2) which can precipitate metals as metal sulfides. The optimum conditions for sulfide production by SRB utilizing lactate, in an upflow anaerobic fixed film reactor (UAFFR) were not previously established. The main objective of this research was to investigate these conditions for the growth of SRB to ensure the highest sulfide production under consistent behaviour of the system. Substrate containing lactate as the organic carbon source along with sulfate, nitrogen and phosphorus as the required nutrients was used as a feed to the UAFER which was seeded with SRB. It was found that an optimum sulfide production occurred with an organic loading rate (OLR) of 6 kg d-1 m-3, while the theoretical oxygen demand to sulfate ratio (ThOD/SO4) ranged from 1.5 to 2.25. Also, the optimum total nitrogen and phosphorus demands were determined to be about 250 and 50 mg L-1 respectively. A total nitrogen concentration above 600 mg L-1 started showing toxicity and lowered the sulfide production. The optimum ThOD:N:P for sulfide production and growth of SRB in the UAFFR was 100:5:1 under optimum conditions.     

Growth performance and physiological responses in striped knifejaw, Oplegnathus fasciatus, held under different photoperiods

The growth performance and stress response in striped knifejaw, Oplegnathus fasciatus (body weight 100–300 g) reared under four photoperiods (6L:6D, 12L:12D, 16L:8D and 24L:0D) were investigated. Fish were fed a commercial diet to apparent satiation, two times a day for 8 weeks. A trial of acute handling and confinement stress was also carried out to investigate the stress-induced levels of different stress indicators in O. fasciatus. Blood was also collected from undisturbed fish which was considered as control. Although there were no significant differences in weight gain, specific growth rate (SGR) and feed conversion efficiency (FCE) in fish exposed to 6L:6D, 16L:8D and 24L:0D photoperiods, all parameters in these photoperiods were significantly higher than those of 12L:12D photoperiod (P < 0.05). There was no significant difference in protein retention efficiency (PRE) between fish exposed to 16L:8D and 24L:0D photoperiods, but PRE in both photoperiods was significantly higher than that of 12L:12D photoperiod.Acute stress significantly increased the plasma levels of cortisol (110.3 ng ml^− 1) and glucose (195.4 mg dl^− 1), and decreased plasma levels of total protein (0.8 g dl^− 1); however, all parameters were returned back to the levels indistinguishable from those of control, undisturbed fish within 24 h. The levels of cortisol, glucose and total protein in fish exposed to different photoperiods during the study were far from the stress-induced levels (P < 0.05). The results demonstrated that the growth performance of O. fasciatus reared from 100 to 300 g can be stimulated significantly by using the manipulated photoperiods where feeding time may be playing an important role to increase food intake and feed conversion efficiency. It also revealed that the artificial photoperiods did not cause significant stress response in fish.