The Political Construction of Free Trade Visions: The Geo-Politics and Geo-Economics of Australian Beef Exporting

This article contributes to emergent scholarship that questions neoliberal discourses in agricultural policy, through a case study that challenges assumptions about the role of “the market” in explaining the recent expansion of Australian beef exports. Australia is the world’s largest beef exporter and its beef exports more than doubled between the mid-1980s and the turn of the 21st century. This expansion, however, can be explained through a particular conjunction of political conditions, which are unlikely to be repeated with equal force in the current decade. Specifically, recent growth hinged upon a combination of (1) rapid growth of Japanese and Korean beef consumption in the early 1990s; (2) the operation of the US “cattle cycle” in the mid-1990s; and (3) the privileged position of Australian beef during this period, vis-à-vis Latin American competition, because of concerns over foot and mouth disease. However, because of their adherence to neoliberal assumptions about the supposed inevitability of trade liberalization, agricultural economists have mistaken these specific circumstances for a general condition in which Australian beef exports will continue growing. This deceptive envisaging of the bounty from liberalization leads not only to false expectations among industry participants, but encourages politics in which industry growth is fallaciously prioritized. Bill Pritchard, PhD, is Senior Lecturer in Economic Geography at the University of Sydney, Australia. He specializes in agri-food globalization.     

Enzyme-linked immunosorbent assay for the pyrethroid deltamethrin

A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of deltamethrin was developed. Two haptens, cyano[3-(4-aminophenoxy)phenyl]methyl 1R-cis-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate and 3-[(+/-)-cyano[1R-cis-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropan ecarbonyloxy]methyl]phenoxyacetic acid, were synthesized and conjugated with thyroglobulin as immunogens. Four antisera were generated and screened against six different coating antigens. The assay that was the most sensitive for deltamethrin was optimized and characterized. The I(50) for deltamethrin was 17.5 +/- 3.6 microg/L, and the lower detection limit was 1.1 +/- 0.5 microg/L. This ELISA assay had relatively low cross-reactivities with other major pyrethroids, such as permethrin, phenothrin, bioresmethrin, cyfluthrin, and cypermethrin. Methanol was found to be the best organic cosolvent for this ELISA, with optimal sensitivity observed at a concentration of 40% (v/v). The assay parameters were unchanged at pH values between 5.0 and 8.0, whereas higher ionic strengths strongly suppressed the absorbances. To increase the sensitivity of the overall method, a C(18) sorbent-based solid-phase extraction was used for river water samples. River water samples fortified with deltamethrin were analyzed according to this method. Good recoveries and correlation with spike levels were observed.     

Comparison of the macrophyte communities of a clearwater and a brownwater oligotrophic lake in Kejimkujik National Park,Nova Scotia

Beaverskin and Pebbleloggitch Lakes are small, proximate, acidic, oligotrophic, headwater lakes located in southwestern Nova Scotia. Beaverskin Lake has clear water, its euphotic zone is deep, and it has extensive beds of macrophytic vegetation that cover most of its bottom to a depth of 6.5 m. In contrast, the water of Pebbleloggitch Lake is highly colored by dissolved humic substances, and macrophytes are restricted to a narrow littoral fringe in depths of less than about 1.8 m. The most widespread macrophyte community in Beaverskin Lake occurs at depths of 2.0–6.5 m and is dominated by Sphagnum macrophyllum and Utricularia vulgaris, while the second most prominent community is littoral and is dominated by Eriocaulon septangulare, Lobelia dortmanna, and Eleocharis acicularis. The most prominent community in Pebbleloggitch Lake is dominated by the floating-leaved Nuphar variegatum rooted as deep as 1.8 m, while communities dominated by E. septangulare and Sphagnum spp. occur in water less than about 0.7 m deep.     

Hapten and antibody production for a sensitive immunoassay determining a human urinary metabolite of the pyrethroid insecticide permethrin

Permethrin is the most popular synthetic pyrethroid insecticide in agriculture and public health. For the development of the enzyme-linked immunosorbent assay (ELISA) to evaluate human exposure to permethrin, the glycine conjugate (DCCA-glycine) of a major metabolite, cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DCCA), of permethrin was established as the target analyte. Four different types of the cis- and trans-isomers of immunizing haptens were synthesized as follows: N-(cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carbonyl)glycine (hapten 3), N-(cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carbonyl)-4-amino-l-phenylalanine (hapten 5), N-(N-(cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carbonyl)glycine)amino-6-(2,4-dinitrophenyl)aminohexanoic acid (hapten 9), and N-(cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carbonyl)glycine-4-oxobutanoic acid (hapten 24). Sixteen polyclonal antibodies produced against each cis- or trans-hapten-thyroglobulin conjugate as immunogens were screened against numerous hapten-bovine serum albumin conjugates as coating antigens. Six ELISAs with both a heterologous hapten structure and a heterologous hapten configuration (cis/trans or trans/cis) between antibody and coating antigen showed a high sensitivity for the target analyte. The IC50 was 1.3, 2.1, and 2.2 microg/L for the trans-target analyte and 0.4, 2.3, and 2.8 microg/L for the cis-target analyte. The immunizing haptens, except for hapten 5, provided the target specific antibodies. Molecular modeling of the haptens supported the selection of reasonable immunizing haptens that best mimicked the target analyte. Hapten 5 was suitable as a coating antigen rather than as an immunogen since it had a different geometry. Very low cross-reactivities were measured to permethrin, its free metabolite (DCCA), PBA-glycine conjugate, and glycine. The ELISA will be optimized for the detection of total cis/trans-DCCA-glycine in human urine samples.     

Determination of pyrethroid residues in agricultural products by an enzyme-linked immunosorbent assay

To determine cypermethrin and permethrin in agricultural products, a competitive enzyme-linked immunosorbent assay (ELISA) method was employed. The matrix interferences were minimized by direct dilution of the extracts. No further cleanup was needed. A minimum matrix effect with a 1:10 dilution of white wine for cypermethrin and a 1:200 dilution of red and white wines, fruits, and vegetables for permethrin was found when phosphate-buffered saline containing 40% methanol was employed as the diluent. Good recoveries of spiked levels were observed. The mean percentage recoveries of cypermethrin spiked in white wine and permethrin spiked in red and white wines were 99.7, 74, and 78%, respectively. The mean percentage recoveries of permethrin spiked in apple, banana, cucumber, lettuce, onion, and peach were 99.2, 105, 70.2, 97.5, 94.4, and 89.4%, respectively. Validation of the ELISA method with permethrin-spiked lettuce and peach was carried out using gas chromatography with mass spectrometry, resulting in a good recovery and correlation.