全文获取类型
收费全文 | 1443篇 |
免费 | 139篇 |
国内免费 | 3篇 |
专业分类
林业 | 96篇 |
农学 | 35篇 |
基础科学 | 7篇 |
176篇 | |
综合类 | 294篇 |
农作物 | 51篇 |
水产渔业 | 193篇 |
畜牧兽医 | 591篇 |
园艺 | 59篇 |
植物保护 | 83篇 |
出版年
2024年 | 3篇 |
2023年 | 26篇 |
2022年 | 32篇 |
2021年 | 45篇 |
2020年 | 62篇 |
2019年 | 72篇 |
2018年 | 65篇 |
2017年 | 57篇 |
2016年 | 62篇 |
2015年 | 40篇 |
2014年 | 56篇 |
2013年 | 75篇 |
2012年 | 101篇 |
2011年 | 154篇 |
2010年 | 81篇 |
2009年 | 48篇 |
2008年 | 97篇 |
2007年 | 83篇 |
2006年 | 70篇 |
2005年 | 62篇 |
2004年 | 72篇 |
2003年 | 62篇 |
2002年 | 51篇 |
2001年 | 11篇 |
2000年 | 8篇 |
1999年 | 6篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 9篇 |
1995年 | 8篇 |
1994年 | 7篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1984年 | 2篇 |
1962年 | 1篇 |
1930年 | 1篇 |
1929年 | 1篇 |
1921年 | 2篇 |
1917年 | 2篇 |
1916年 | 2篇 |
1914年 | 1篇 |
1913年 | 2篇 |
1909年 | 1篇 |
1906年 | 1篇 |
1903年 | 1篇 |
1902年 | 1篇 |
排序方式: 共有1585条查询结果,搜索用时 765 毫秒
141.
Trifilo MJ Yajima T Gu Y Dalton N Peterson KL Race RE Meade-White K Portis JL Masliah E Knowlton KU Chesebro B Oldstone MB 《Science (New York, N.Y.)》2006,313(5783):94-97
We investigated extraneural manifestations in scrapie-infected transgenic mice expressing prion protein lacking the glycophosphatydylinositol membrane anchor. In the brain, blood, and heart, both abnormal protease-resistant prion protein (PrPres) and prion infectivity were readily detected by immunoblot and by inoculation into nontransgenic recipients. The titer of infectious scrapie in blood plasma exceeded 10(7) 50% infectious doses per milliliter. The hearts of these transgenic mice contained PrPres-positive amyloid deposits that led to myocardial stiffness and cardiac disease. 相似文献
142.
143.
144.
CASE DESCRIPTION: A 6-year-old female African pygmy hedgehog was evaluated because of a mass of unknown duration on the lateral aspect of the right shoulder region. CLINICAL FINDINGS: A fine-needle aspirate of the mass was collected for cytologic examination; findings were consistent with a spindle cell tumor. A CBC, plasma biochemical analyses, and whole-body radiography revealed no other abnormalities. TREATMENT AND OUTCOME: An initial surgery performed in an attempt to remove the mass with preservation of the limb failed in that tumor-free surgical margins were not obtained. Histologically, the mass was identified as a peripheral nerve sheath tumor (neurofibrosarcoma). A second surgery to amputate the forelimb was successful. After 1 year, there had been no further development of peripheral nerve sheath tumor at this or other sites. CLINICAL RELEVANCE: In African pygmy hedgehogs, potential differential diagnoses for a subcutaneous mass should include peripheral nerve sheath tumor. If necessary, forelimb amputation can be performed successfully in this species with procedures modified from those used in dogs. Information gathered during the treatment and recovery of the hedgehog of this report may assist practicing veterinarians in counseling owners of hedgehogs that are undergoing forelimb amputation with regard to the course of recovery that may be expected following this procedure. 相似文献
145.
Trinh Xuan Hoat Ngo Gia Bon Mai Van Quan Vu Duy Hien Nguyen Duc Thanh Matthew Dickinson 《Phytoparasitica》2012,40(4):351-359
Sugarcane is an important cash crop in Vietnam and has been widely promoted at national and provincial level. In 2006, a new disease was discovered in sugarcane in the Nghean Tate&Lyle Sugar Mill in Nghean Province in north-central Vietnam. The key symptoms were the formation of green grassy shoots around the base of mature stools. We applied nested polymerase chain reaction (PCR) using P1/P7 and R16F2n/R16R2 for detection and characterization of phytoplasma from the symptomatic tissues. PCR products of the expected size (approx. 1200?bp) were obtained from the 16S rDNA of the phytoplasma. The restriction fragment length polymorphism profiles indicated that all samples were infected by the same phytoplasma. Phylogenetic analysis confirmed that the SCGS phytoplasma from Vietnam belong to the 16SrXI group, formerly Rice Yellow Dwarf group. 相似文献
146.
Price DC Chan CX Yoon HS Yang EC Qiu H Weber AP Schwacke R Gross J Blouin NA Lane C Reyes-Prieto A Durnford DG Neilson JA Lang BF Burger G Steiner JM Löffelhardt W Meuser JE Posewitz MC Ball S Arias MC Henrissat B Coutinho PM Rensing SA Symeonidi A Doddapaneni H Green BR Rajah VD Boore J Bhattacharya D 《Science (New York, N.Y.)》2012,335(6070):843-847
147.
Peter T. Birteeb Sunday Olusola Peters Abdulmojeed Yakubu Matthew Adekunle Adeleke Michael Ohiokhuaobo Ozoje 《Tropical animal health and production》2012,45(1):267-274
The characterisation of the small ruminant populations in developing countries will play a major role in the maintenance of the genetic resources as the basis for future improvement in livestock production. The present study aimed at morphological characterisation of the two main breeds of sheep in Ghana by assessing variation within and between breed populations using principal component and discriminant analyses. The two breeds were the Sahel and the Djallonke sheep of both sexes and of two groups namely, young (1 year old, consisting of 74 animals) and mature sheep (≥2 years old, comprising 219 animals). The analysis of variance revealed significant (P?<?0.05) differences in the morphological traits of the Sahel and the Djallonke sheep breeds with higher values recorded for the former. Sexual dimorphism was in favour of male animals in all the morphological traits examined. Mature animals also had comparative advantage over the young. Two principal components were extracted to discern the structure of the two genetic groups. The most discriminating traits between the two sheep breeds were rump height, height at withers, neck girth and pin-bone width. Mahalanobis distance between the two genetic groups was 5.723 (P?<?0.0001). The developed discriminant functions clearly discriminated and classified the Sahel and the Djallonke sheep into their breeds of origin, thus yielding 100, 93.4 and 90.4 % accurate classification for the rams, ewes and the overall sheep population, respectively. The present approach would greatly help in establishing management and conservation policies for the sustainable production of the two Ghanaian sheep breeds. 相似文献
148.
Meyerhoff RR Nighot PK Ali RA Blikslager AT Koci MD 《Comparative immunology, microbiology and infectious diseases》2012,35(1):63-69
The inducible nitric oxide synthase (iNOS) enzyme has long been recognized as a key mediator of innate immune responses to infectious diseases across the phyla. Its role in killing or inactivating bacterial, parasitic, and viral pathogens has been documented in numerous host systems. iNOS, and its innate immune mediator NO has also been described to have negative consequence on host tissues as well; therefore understanding the pathogenesis of any infectious agent which induces iNOS expression requires a better understanding of the role iNOS and NO play in that disease. Previous studies in our laboratory and others have demonstrated evidence for increased levels of iNOS and activity of its innate immune mediator NO in the intestine of turkeys infected with astrovirus. To begin to characterize the role iNOS plays in the innate immune response to astrovirus infection, we identified, characterized, developed tkiNOS specific reagents, and demonstrated that the intestinal epithelial cells induce expression of iNOS following astrovirus infection. These data are the first to our knowledge to describe the tkiNOS gene, and demonstrate that astrovirus infection induces intestinal epithelial cells to express iNOS, suggesting these cells play a key role in the antiviral response to enteric infections. 相似文献
149.
150.
During the past decade the British livestock industry has suffered from several major pathogen outbreaks, and a variety of regulatory and disease control measures have been applied to the movement of livestock with the express aim of mitigating the spread of infection. The Rapid Analysis and Detection of Animal-related Risks (RADAR) project, which has been collecting data on the movement of cattle since 1998, provides a relatively comprehensive record of how these policies have influenced the movement of cattle between animal holdings, markets, and slaughterhouses in Britain. Many previous studies have focused on the properties of the network that can be derived from these movements--treating farms as nodes and movements as directed (and potentially weighted) edges in the network. However, of far greater importance is how these policy changes have influenced the potential spread of infectious diseases. Here we use a stochastic fully individual-based model of cattle in Britain to assess how the epidemic potential has varied from 2000 to 2009 as the pattern of movements has changed in response to legislation and market forces. Our simulations show that the majority of policy changes lead to significant decreases in the epidemic potential (measured in multiple ways), but that this potential then increases through time as cattle farmers modify their behaviour in response. Our results suggest that the cattle industry is likely to experience boom-bust dynamics, with the actions that farmers take during epidemic-free periods to maximise their profitability likely to increase the potential for large-scale epidemics to occur. 相似文献