全文获取类型
收费全文 | 349篇 |
免费 | 40篇 |
国内免费 | 2篇 |
专业分类
林业 | 16篇 |
农学 | 6篇 |
31篇 | |
综合类 | 41篇 |
农作物 | 20篇 |
水产渔业 | 11篇 |
畜牧兽医 | 231篇 |
园艺 | 11篇 |
植物保护 | 24篇 |
出版年
2023年 | 5篇 |
2022年 | 4篇 |
2021年 | 7篇 |
2020年 | 15篇 |
2019年 | 10篇 |
2018年 | 16篇 |
2017年 | 15篇 |
2016年 | 13篇 |
2015年 | 8篇 |
2014年 | 24篇 |
2013年 | 21篇 |
2012年 | 13篇 |
2011年 | 21篇 |
2010年 | 12篇 |
2009年 | 22篇 |
2008年 | 11篇 |
2007年 | 9篇 |
2006年 | 14篇 |
2005年 | 14篇 |
2004年 | 10篇 |
2003年 | 6篇 |
2002年 | 11篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 17篇 |
1997年 | 12篇 |
1996年 | 12篇 |
1995年 | 11篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1945年 | 1篇 |
排序方式: 共有391条查询结果,搜索用时 859 毫秒
351.
Background
Studies of host-parasite interactions have the potential to provide insights into the ecology of both organisms involved. We monitored the movement of sucking lice (Lemurpediculus verruculosus), parasites that require direct host-host contact to be transferred, in their host population of wild mouse lemurs (Microcebus rufus). These lemurs live in the rainforests of Madagascar, are small (40 g), arboreal, nocturnal, solitary foraging primates for which data on population-wide interactions are difficult to obtain. We developed a simple, cost effective method exploiting the intimate relationship between louse and lemur, whereby individual lice were marked, without removal from their host, with an individualized code, and tracked throughout the lemur population. We then tested the hypotheses that 1) the frequency of louse transfers, and thus interactions, would decrease with increasing distance between paired individual lemurs; 2) due to host polygynandry, social interactions and hence louse transfers would increase during the onset of the breeding season; and 3) individual mouse lemurs would vary in their contributions to the spread of lice.Results
We show that louse transfers involved 43.75% of the studied lemur population, exclusively males. Louse transfers peaked during the breeding season, perhaps due to increased social interactions between lemurs. Although trap-based individual lemur ranging patterns are restricted, louse transfer rate does not correlate with the distance between lemur trapping locales, indicating wider host ranging behavior and a greater risk of rapid population-wide pathogen transmission than predicted by standard trapping data alone. Furthermore, relatively few lemur individuals contributed disproportionately to the rapid spread of lice throughout the population.Conclusions
Using a simple method, we were able to visualize exchanges of lice in a population of cryptic wild primates. This method not only provided insight into the previously unseen parasite movement between lemurs, but also allowed us to infer social interactions between them. As lice are known pathogen vectors, our method also allowed us to identify the lemurs most likely to facilitate louse-mediated epidemics. Our approach demonstrates the potential to uncover otherwise inaccessible parasite-host, and host social interaction data in any trappable species parasitized by sucking lice. 相似文献352.
Vitour D Guillotin J Sailleau C Viarouge C Desprat A Wolff F Belbis G Durand B Bakkali-Kassimi L Breard E Zientara S Zanella G 《Veterinary research》2011,42(1):18
Since its introduction into northern Europe in 2006, bluetongue has become a major threat to animal health. While the efficacy of commercial vaccines has been clearly demonstrated in livestock, little is known regarding the effect of maternal immunity on vaccinal efficacy. Here, we have investigated the duration and amplitude of colostral antibody-induced immunity in calves born to dams vaccinated against bluetongue virus serotype 8 (BTV-8) and the extent of colostral antibody-induced interference of vaccination in these calves. Twenty-two calf-cow pairs were included in this survey. The median age at which calves became seronegative for BTV was 84 and 112 days as assayed by seroneutralisation test (SNT) and VP7 BTV competitive ELISA (cELISA), respectively. At the mean age of 118 days, 13/22 calves were immunized with inactivated BTV-8 vaccine. In most calves vaccination elicited a weak immune response, with seroconversion in only 3/13 calves. The amplitude of the humoral response to vaccination was inversely proportional to the maternal antibody level prior to vaccination. Thus, the lack of response was attributed to the persistence of virus-specific colostral antibodies that interfered with the induction of the immune response. These data suggest that the recommended age for vaccination of calves born to vaccinated dams needs to be adjusted in order to optimize vaccinal efficacy. 相似文献
353.
Antonius JM Matzke Koichi Watanabe Johannes van der Winden Ulf Naumann Marjori Matzke 《Plant methods》2010,6(1):2
Background
Interphase chromosome organization and dynamics can be studied in living cells using fluorescent tagging techniques that exploit bacterial operator/repressor systems and auto-fluorescent proteins. A nuclear-localized Repressor Protein-Fluorescent Protein (RP-FP) fusion protein binds to operator repeats integrated as transgene arrays at defined locations in the genome. Under a fluorescence microscope, the tagged sites appear as bright fluorescent dots in living cells. This technique has been used successfully in plants, but is often hampered by low expression of genes encoding RP-FP fusion proteins, perhaps owing to one or more gene silencing mechanisms that are prevalent in plant cells. 相似文献354.
CA Meza‐Herrera A Gonzalez‐Bulnes RT Kridli M Mellado CF Arechiga‐Flores H Salinas JM Luginbuhl 《Reproduction in domestic animals》2010,45(6):e495-e502
Puberty is the result of a dynamic interaction between genetic factors and environmental cues, all of which lead to the attainment of reproductive capacity. Thus, significant changes in hormone secretion occur from the pre‐pubertal to the pubertal stage. The objective of this review is to provide an update of some endocrine, physiological, metabolic and genetic concepts involved in the establishment of the hypothalamic‐hypophyseal‐gonadal axis function promoting the onset of the reproductive function during puberty. To achieve this purpose, basic aspects of the function of the hypothalamic‐hypophyseal‐gonadal axis, the control of the axis by neurotransmitters and the interaction between reproductive function and metabolic status will be considered. Finally, the role of the novel kisspeptin system and the GPR54 receptor as modulators of puberty will be considered, in addition to the hierarchical expression of the main genes acting as regulators of the onset of puberty. 相似文献
355.
356.
Melioidosis in a dog 总被引:1,自引:0,他引:1
357.
Forty sheep and 40 heifers were dosed with an intraruminal slow release capsule (IRSRC) constructed to deliver albendazole (ABZ) at a low daily dosage for three months. Blood samples were collected at standardised intervals for 110 days and analysed by high performance liquid chromatography for the quantification of the two main metabolites sulphoxide (SO.ABZ) and sulphone (SO2ABZ). The plasma profiles show sustained concentrations of the active metabolite SO.ABZ for 105 days in sheep (m = 0.06 +/- 0.032 micrograms ml-1) and 85 days in cattle (m = 0.10 +/- 0.019 micrograms ml-1). In both species, the proportions of the metabolites were inverted compared to that observed after a single dosage. The bioavailability of ABZ after the administration of the IRSRC compared with a drench was reduced in sheep but increased in cattle. The IRSRC exhibited a preventive and therapeutic effect for at least three months. 相似文献
358.
359.
360.
SUMMARY Reproductive performance was compared between cows whose previous parturition was induced and non-induced cows with similar calving dates, in 49 winter-calving, pasture-fed, commercial dairy herds in south-western Victoria. Parturition was induced in winter when most cows were between 27 and 35 weeks of pregnancy. Reproductive performance was assessed during the next mating period after induction which was mainly in spring of the same year. Percentages of cows in induced and untreated groups that were not pregnant after the mating period (9.0% and 7.2%, respectively) did not differ significantly. Induction tended to increase the percentage of cows of unknown pregnancy status. Mean percentages for induced and untreated groups were 11.5% and 7.9%, respectively. Induced and untreated groups calved at similar intervals after the planned start of calving in the following year, and the percentages of groups that required induction in that year did not differ significantly. The direct effects of induced parturition on reproduction were therefore concluded to be minimal. In seasonal calving herds, improvements in reproductive performance could be expected among cows whose calving dates were altered substantially by induction, due to increased intervals from calving to mating start date . 相似文献