The DMAC protocol (dexamethasone, melphalan, actinomycin‐D, cytarabine) has been evaluated in American studies for the treatment of relapsed canine lymphoma, comparing similarly to other rescue protocols. The aim of this study was to evaluate efficacy and toxicity of DMAC, in a larger UK cohort of resistant canine lymphomas. Medical records of dogs with resistant non‐Hodgkin high‐grade lymphomas that received DMAC as a rescue protocol were reviewed from 2007 to 2017. Response, time from initiation to discontinuation (TTD) and toxicity (Veterinary Cooperative Oncology Group criteria) were assessed. One hundred dogs were included; 86 received CEOP (modified CHOP including epirubicin) as first‐line treatment. Thirty‐five dogs (35%) responded: 21 complete responders (CRs) and 14 partial responders (PRs). Responders had significantly longer TTD (P < 0.001) compared with non‐responders: 62 days (range 28‐952) for CR vs 32 days (range 20‐70) for PR. Six CR received more than six cycles of DMAC (range 7‐36 cycles) and experienced a longer TTD (median 508, range 126‐952 days). Thrombocytopenia occurred in 45% (24 grade 1‐2, 21 grade 3‐4) and neutropenia in 36% of cases (29 grade 1‐2, 7 grade 3‐4). Gastrointestinal toxicity occurred in 42% of dogs (40 grade 1‐2, 2 grade 3‐4). Owing to chemotherapy toxicity, treatment was discontinued in five, and hospitalization required in six cases. In this study, response to DMAC was lower and of generally shorter duration than previously reported. Toxicity was high, but infrequently led to hospitalization or discontinuation of treatment. 相似文献
Species range expansion reduces genetic variation at the margins of a species range and should thus compromise the adaptive potential of its marginal populations. Remarkably, this prediction has not previously been tested. Here, we show that populations of the plant Mercurialis annua, which expanded its range into Spain and Portugal from North Africa after the Pleistocene glaciation, respond to selection on a key life-history trait less well than populations from the species' historical refugium. Our results provide direct evidence of a decline in adaptive potential across the geographic range of a species after a shift in its distribution. Predicting evolutionary responses to environmental change will thus need to account for the genetic heterogeneity of species and the spatial dynamics of their geographic distributions. 相似文献
Commercially‐exploited fish populations are not only shaped by fishing pressure, but also by the dynamics of their environment. By quantifying the influence of environmental variability, fisheries management advice can be improved and uncertainties reduced. To this end, we developed statistical models of the response of Scotian Shelf silver hake stock metrics to variability in zooplankton community composition and phenology over the past 18 years and in the physical environment since 1985. Dominant modes of variability in these pelagic habitat indicators were characterized using principal component analysis, and the relationships of silver hake condition, abundance, and recruitment to pelagic habitat variability were assessed using generalized additive models. Condition was largely modulated by the onset and duration of the spring bloom, which controls food availability. In contrast, adult abundance was governed by composition of the zooplankton community and bottom‐water temperature, which dictates the distribution of silver hake. Finally, recruitment was affected by both thermal conditions and food availability. Our results presented here form the basis for qualitative assessment of ecosystem attributes and the influence on silver hake stock productivity. 相似文献
Five severe cases of psittacosis in individuals associated with duck farms were notified in France between January and March 2006. Diagnostic examination included serology and/or molecular detection by PCR from respiratory samples. As a consequence, we investigated all duck flocks (n=11) that were housed in the three farms where human infections occurred. While serology by complement fixation test was negative for all samples, cloacal and/or tracheal chlamydial excretion was detected by PCR in all three units. Notably, one duck flock was tested strongly positive in 2 of the 3 affected farms, and Chlamydophila (C.) psittaci strains were isolated from cloacal and/or tracheal swab samples from both farms. Human samples and duck isolates exhibited the same PCR-RFLP restriction pattern, which appeared to be an intermediate between genotypes A and B. Analysis of ompA gene sequences and comparison to those of the type strains showed that the isolates could not be strictly assigned to any of the generally accepted genotypes of C. psittaci. Further analysis by MLVA of the PCR-positive human samples revealed two distinct patterns, which were related to previously isolated C. psittaci duck strains. 相似文献
Changes to ecosystems caused by introduced herbivores can be predictable, stepwise transitions or unpredictable and even irreversible state changes. This study's objectives were to explore effects on forest succession and soil development 5 years after moose (Alces alces L.) were fenced out of areas within and adjacent to a national park in Newfoundland, Canada. Study plots spanned a range of understorey broadleaf plant associations with regenerating balsam fir (Abies balsamea (L.) Mill.), an important winter forage plant for moose and a dominant canopy tree throughout Newfoundland. After 5 years, height–diameter ratios were significantly larger for larger basal diameters of understorey balsam fir in unfenced, but not in fenced subplots, suggesting that growth of the conifer is compromised within the exclosure. In contrast, for most broadleaf trees and shrubs, moose removal by fencing results in greater heights and basal diameters than in control subplots. The competitive advantage of broadleaf trees and shrubs over balsam fir in the short-term may be a result of past sustained heavy moose browsing benefiting plants that are better at investing resources into below-ground growth or benefiting plants that have broader leaf canopies. It is not clear how long the broadleaf transition state we document will continue. Restorative actions intended to mimic usual patterns of forest regeneration in this region of Newfoundland might best consider moose removal with site preparation and/or planting to historic densities. 相似文献
Infratentorial tumors are relatively infrequent in dogs and a lack of data makes it difficult to offer prognostic information. Untreated, dogs with these neoplasms have shorter survival times than those with supratentorial tumors. The role of radiation therapy (RT) in the management of infratentorial tumors is poorly defined and tumoral/peritumoral swelling in this site is a potential cause of serious acute side effects. The aim of this retrospective, cohort study was to describe cases of infratentorial tumors treated with fractionated three‐dimensional conformal RT (3D CRT) and glucocorticoids (GC), and compare outcomes and survival with dogs affected by tumors in the same location that received GC alone. Thirty patients with a MRI diagnosis of infratentorial tumors were recruited (15 received RT and GC and 15 GC alone). None had mentation changes at presentation. For both groups, MRI and medical records were reviewed; and factors associated with survival were evaluated with Kaplan–Meier product limit survival and Cox regression analysis. Overall median survival time (MST) was 294 days (95% CI 42–545). The MST in the RT group was 756 days (95% CI 209–1302) vs. 89 days (95% CI 34.7–143.3 days) for those dogs treated palliatively with GC alone. This difference was statistically significant (P = 0.001). No other factors (including neurological signs, MRI features, tumor volume and total RT dose) were statistically associated with survival in the RT group. This study suggests that 3D CRT offers a survival advantage for dogs with infratentorial tumors compared to GC alone, and significant complications are uncommon. 相似文献
The cover image, by E. Treggiari et al., is based on the Original Article Clinical outcome, PDGFRß and KIT expression in feline histiocytic disorders: a multicentre study, DOI: 10.1111/vco.12142 .
A study was conducted to establish a growth rate response of potato plant (Solanum tuberosum L.) tops to exposure to 72-hr periods of six constant temperature treatments (10, 15, 20, 25, 30, 35°C) and to determine a thermal optimum for vegetative potato growth rates. The plants were maintained under “no stress” soil moisture conditions throughout the study. The temperature treatments were accomplished by inserting the plants into a growth chamber for 72 hours at selected temperatures. Maximum growth (as measured by percent change in leaf area) occurred at 25°C with temperature growth differences significant at the 5% level. Plant height measurements (percent change occurring during treatment time) showed maximum stem elongation at 30°C during an early growth stage and 25°C during stage 2 (4 weeks later). Predictive models developed from the data indicate maximum rates of leaf area increase in both growth stages occur at 24.7°C and that maximum rates of stem elongation occur at 31.3 and 27.4°C for growth stages 1 and 2, respectively. Leaf water potential and stomatal resistance measurements taken at the end of each treatment period indicated that no water stress occurred. 相似文献
A female French bulldog was presented with an enlarged clitoris. Abdominal surgery revealed a normal uterus and gonads resembling testes. Histologically, the gonads contained seminiferous tubules. The karyotype was XX, and the SRY gene was not detected. A diagnosis of XX male, SRY negative disorder of sexual development was made. 相似文献
A study determined the effects of supplementing corn-based diets for weaned pigs with multi-enzymes on growth performance, apparent total tract digestibility (ATTD) of nutrients, fecal score, and fecal microbial composition. A total of 132 pigs (initial body weight = 7.23 kg) that had been weaned at 21 d of age and fed a drug-free nursery diet for 7 d were housed in 33 pens of 4 barrows or gilts, blocked by body weight and gender, and fed 3 experimental diets at 11 pens per diet. The diets were corn-based diet without or with multi-enzyme A or B. Multi-enzyme A supplied 4,000 U of xylanase, 150 U of β-glucanase, 3,500 U of protease, and 1,500 U of amylase per kilogram of diet. Multi-enzyme B was the same as multi-enzyme A except that it supplied amylase at 150 U/kg, and that its source of amylase was different from that of multi-enzyme A. All diets contained phytase at 1,000 U/kg. The diets were fed for 35 d in 2 phases; phase 1 for the first 14 d and phase 2 for the last 21 d of the trial. Fecal score was determined daily during the first 7 d of the trial. Fecal samples were collected from rectum of 1 pig per pen on days 2, 7, 14, and 35 of the trial for determining bacterial composition. Also, fresh fecal samples were collected from each pen on days 41 and 42 to determine ATTD of nutrients. Multi-enzyme B increased (P < 0.05) average daily gain (ADG) for phases 1 and 2. For the overall study period, multi-enzyme B increased (P < 0.05) ADG from 262 to 313 g, and average daily feed intake (ADFI) from 419 to 504 g. Multi-enzyme A increased (P < 0.05) overall ADG from 262 to 290 g, but did not affect ADFI. Multi-enzyme A or B did not affect ATTD of gross energy, but increased (P < 0.05) the ATTD of ether extract from 30% to 36% or 37%, respectively. Multi-enzyme A did not affect fecal score; however, multi-enzyme B tended to decrease (P = 0.09) fecal score, implying that it tended to decrease diarrhea. Firmicutes were the most abundant phylum of fecal bacteria (its relative abundance ranged from 58% to 72%). Bacteroidetes and Actinobacteria were the 2nd and 3rd most abundant phyla of fecal bacteria. Neither multi-enzyme affected fecal bacterial composition. In conclusion, the addition of multi-enzyme A or B to phytase-supplemented corn-based diet for weaned pigs can improve their growth performance and fat digestibility. However, multi-enzyme B was more effective than multi-enzyme A in terms of improving the growth performance of weaned pigs fed corn-based diet. 相似文献