改进培养方法提高组培快繁技术的初探

通过简化培养基成分，去除有机物、琼脂等，可降低试管苗成本；采用自然光替代日光灯，并结合黑暗培养，可减少设备投入．有利于工厂化育苗生产。     

Retrospective assessment of tolerability and efficacy of zoledronate in the palliative treatment of cancer-bearing dogs

Zoledronate is a bisphosphonate frequently used for the treatment of hypercalcaemia of malignancy and tumour-associated bone pain in dogs, however, there is a paucity of information regarding its use in veterinary medicine. The aim of this retrospective study was to report the tolerability of zoledronate in the palliative treatment of cancer-bearing dogs and secondarily to to assess the efficacy of zoledronate for the treatment of hypercalcaemia of malignancy. Thirty-seven dogs (22 with tumour-associated bone pain and 15 with hypercalcaemia of malignancy) that received 114 zoledronate infusions were included. Tolerability was assessed by the absence of post-zoledronate hypocalcaemia or other adverse events as defined by Veterinary Cooperative Oncology Group-Common Terminology Criteria for Adverse Events criteria. Efficacy was assessed by comparison of available ionized calcium levels before and after zoledronate administration in hypercalcaemic dogs. In 79% of zoledronate infusions, no adverse events were reported. The majority of adverse events which occurred in the other 21% of infusions could be attributed to concurrent chemotherapy or the underlying neoplastic disease. There was a small but significant increase in creatinine following treatment with zoledronate, however, none of the dogs developed clinically significant renal disease. In eight hypercalcaemic dogs with available ionized calcium following zoledronate administration, ionized calcium decreased rapidly within 7 days following treatment with zoledronate. Zoledronate is well-tolerated with few recorded adverse events, however, monitoring of serum creatinine is advised. Zoledronate seems to be effective in the treatment of hypercalcaemia of malignancy.     

Artificial insemination in tench, Tinca tinca L.

Abstract. The sperm of tench, Tinca tinca L., is characterized by a milky colour and consistency, and is of very low density. After collecting the sperm, motion of spermatozoa was recorded even without water activation. A better motility value (value 4·36 on average) was observed in spermatozoa collected in immobilizing solution (collecting medium) and stored for 3h, when compared with spermatozoa without collecting medium. Average total and relative numbers of spermatozoa were 12·16 × 10⁹ per male and 18·50 × 10⁹ per kg of body weight, respectively. When testing the effect of activating solution in artificial propagation of tench, the highest fertilization rates (81·3 and 85% in two cases) were found for NaCl solution with an osmotic concentration of 34 or 69 mOsmol and for fresh water, respectively. The fertility rate was reduced significantly ( P < 0·01) by any increase above 105 mOsmol in NaCl concentration in the activating solution. In the tests of optimal method of artificial fertilization, the highest hatching rate of sac fry (71·35%) was found in sperm collected into immobilizing solution. The application of immobilizing solution significantly increased the number of sac fry at the levels P < 0·1 and P < 0·01, if compared with intact sperm stored for 3 h and fresh sperm, respectively.     

Phytosequestration of Carbon in Miscanthus × giganteus and Pinus sylvestris L. in Degraded Zinc Smelter and Post-Mining Soils

正Dear Editor,Pinus sylvestris L.is one of the most popular and predominant tree species in Central Europe and Scandinavia.Its cultivation depends on atmospheric conditions,soil fertility,use of fertilizers,and individual characteristics of the trees.Pinus sylvestris L.wood,roots,and needles are used for energy production.Pi-     

道塞特、萨福克与阿勒泰羊5月龄杂交公羔肉品质分析

选取5月龄萨福克×阿勒泰(SAF1、SAF2)、道塞特×阿勒泰(DAF1、DAF2)杂交一、二代羔羊与同龄阿勒泰羊(ALT)各3只,对肉品质进行分析。结果表明:5月龄杂交羔羊肉品质得到明显改善,特别是蛋白质、脂肪和氨基酸含量变化尤为明显,且杂交二代优于杂交一代,杂交组合SA优于DA。     

新疆褐牛体尺性状指标与体重的主成分分析

选择30头18月龄左右新疆褐牛进行体尺、体重测定,选择累计贡献率达89.505%的7个主成分进行分析。结果表明,新疆褐牛体尺指标尻长、腰角宽、髋宽、坐骨宽及体重的变异较大,选育潜力大;体重与体斜长、胸围、腰角宽、髋宽等指标存在极显著相关（P＜0.01）,与管围呈显著相关（P＜0.05）;从主成分的特征根与累计贡献率来看,第1至第3主成分起主导作用的第1主成分主要反映体型外貌特征,第2主成分主要反映尻部特征,第3主成分主要反映后躯特征。