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排序方式: 共有126条查询结果,搜索用时 254 毫秒
31.
OBJECTIVE: To investigate penetration of a topically applied nonsteroidal anti-inflammatory drug (NSAID) into tissues and synovial fluid. ANIMALS: 5 Greyhounds. PROCEDURE: Dogs were anesthetized and microdialysis probes placed in the dermis and gluteal muscle over each coxofemoral (hip) joint. Methylsalicylate (MeSA) was applied topically over the left hip joint. Dialysate and plasma (blood samples from the cephalic and femoral veins) were obtained during the subsequent 5 hours. Dogs were euthanatized, and tissue samples and synovial fluid were collected and analyzed for salicylic acid (SA) and MeSA by use of high-pressure liquid chromatography. RESULTS: SA and MeSA concentrations increased rapidly (< 30 minutes after application) in dialysate obtained from treated dermis. Salicylic acid also appeared in plasma within 30 minutes and reached a plateau concentration after 2 hours, although combined drug concentrations (SA plus MeSA) in plasma obtained from femoral vein samples were twice those measured in plasma obtained from the cephalic vein (SA only). Treated muscle had a progressive decrease in NSAID concentration with increasing depth (SA and MeSA), but it was significantly higher than the concentration in untreated muscle. Substantial amounts of SA and MeSA were also measured in synovial fluid of treated joints. CONCLUSIONS AND CLINICAL RELEVANCE: Topically applied NSAIDs can penetrate deeply into tissues and synovial fluid. Local concentrations higher than circulating systemic concentrations are suggestive that direct diffusion and local blood redistribution are contributing to this effect. Systemic blood concentrations may be inadequate to describe regional kinetics of topically applied drugs. 相似文献
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33.
Domenico Caivano Antonello Bufalari Maria Elena Giorgi Maria Beatrice Conti Maria Chiara Marchesi Giovanni Angeli Francesco Porciello Francesco Birettoni 《Veterinary radiology & ultrasound》2014,55(5):561-564
A 3‐year‐old English Setter dog was presented for an acute onset of coughing. Tracheobronchoscopic examination allowed localization and removal of one grass awn foreign body. A second migrated grass awn was suspected to be present in the left caudal lung lobe. Transesophageal ultrasound revealed an area of pulmonary consolidation in the dorsomedial portion of left caudal lobe and a linear hyperechoic structure consistent with a grass awn foreign body within the area of consolidation. Transesophageal ultrasonography was also used to provide anatomical landmarks that facilitated successful thoracoscopic removal of the foreign body. 相似文献
34.
Sillaber I Rammes G Zimmermann S Mahal B Zieglgänsberger W Wurst W Holsboer F Spanagel R 《Science (New York, N.Y.)》2002,296(5569):931-933
There is a relation between stress and alcohol drinking. We show that the corticotropin-releasing hormone (CRH) system that mediates endocrine and behavioral responses to stress plays a role in the control of long-term alcohol drinking. In mice lacking a functional CRH1 receptor, stress leads to enhanced and progressively increasing alcohol intake. The effect of repeated stress on alcohol drinking behavior appeared with a delay and persisted throughout life. It was associated with an up-regulation of the N-methyl-d-aspartate receptor subunit NR2B. Alterations in the CRH1 receptor gene and adaptional changes in NR2B subunits may constitute a genetic risk factor for stress-induced alcohol drinking and alcoholism. 相似文献
35.
Retina: ultrastructural alterations produced by extremely low levels of coherent radiation 总被引:1,自引:0,他引:1
The effect of low levels of coherent radiation on the eye is not fully established, but is generally presumed to be noninjurious. Irradiation of the retina with a Q-switched ruby laser emitting low amounts of energy (0.1 percent probability of creating damage) consistently produces ultrastructural alterations in rods and cones. Outer segments of these cells are broken and disorganized and their lamellae are in disarray 1 day after such irradiation. 相似文献
36.
OBJECTIVE: To evaluate the efficacy of a community-based dietary intervention to reduce risk of micronutrient inadequacies in high-phytate maize-based Malawian diets. DESIGN: Quasi-experimental post-test design with a non-equivalent control group. SETTING: Four villages in Mangochi District, Southern Malawi. PARTICIPANTS: Households with children aged 3-7 years in two intervention (n = 200) and two control (n = 81) villages participated in a 6-month intervention employing dietary diversification, changes in food selection patterns, and modifications to food processing to reduce the phytate content of maize-based diets. Baseline comparability between the groups was confirmed via assessment of sociodemographic characteristics, anthropometry, knowledge and practices, morbidity, haemoglobin and hair zinc. After 12 months, knowledge and practices and dietary intakes were assessed by interactive 24-hour recalls, one during the food plenty and a second during the food shortage season. Nutrient adequacy for the two groups was compared via dietary quality indicators and predicted prevalence of inadequate intakes using the probability approach. RESULTS: Intervention children had diets that were significantly more diverse and of a higher quality than those of controls. Median daily intakes of protein, calcium, zinc (total and available), haem iron, vitamin B12 and animal foods (grams; % of total energy) were higher (P < 0.05) whereas phytate intakes, phytate/zinc and phytate/iron molar ratios were lower (P < 0.01) in the intervention group; some spread of knowledge and practices to controls occurred. CONCLUSIONS: Our community-based dietary strategies reduced the predicted prevalence of inadequate intakes of protein, calcium, zinc and vitamin B12, but not iron, in children from Malawian households with very limited resources. 相似文献
37.
Moreno A Di Trani L Faccini S Vaccari G Nigrelli D Boniotti MB Falcone E Boni A Chiapponi C Sozzi E Cordioli P 《Veterinary microbiology》2011,149(3-4):472-477
Swine influenza monitoring programs have been in place in Italy since the 1990 s and from 2009 testing for the pandemic H1N1/2009 virus (H1N1pdm) was also performed on all the swine samples positive for type A influenza. This paper reports the isolation and genomic characterization of a novel H1N2 swine influenza reassortant strain from pigs in Italy that was derived from the H1N1pdm virus. In May 2010, mild respiratory symptoms were observed in around 10% of the pigs raised on a fattening farm in Italy. Lung homogenate taken from one pig showing respiratory distress was tested for influenza type A and H1N1pdm by two real time RT-PCR assays. Virus isolation was achieved by inoculation of lung homogenate into specific pathogen free chicken embryonated eggs (SPF CEE) and applied onto Caco-2 cells and then the complete genome sequencing and phylogenetic analysis was performed from the CEE isolate. The lung homogenate proved to be positive for both influenza type A (gene M) and H1N1pdm real time RT-PCRs. Virus isolation (A/Sw/It/116114/2010) was obtained from both SPF CEE and Caco-2 cells. Phylogenetic analysis showed that all of the genes of A/Sw/It/116114/2010, with the exception of neuraminidase (NA), belonged to the H1N1pdm cluster. The NA was closely related to two H1N2 double reassortant swine influenza viruses (SIVs), previously isolated in Sweden and Italy. NA sequences for these three strains were clustering with H3N2 SIVs. The emergence of a novel reassortant H1N2 strain derived from H1N1pdm in swine in Italy raises further concerns about whether these viruses will become established in pigs. The new reassortant not only represents a pandemic (zoonotic) threat but also has unknown livestock implications for the European swine industry. 相似文献
38.
Beatrice Pezzarossa Diletta Piccotino Carol Shennan Fernando Malorgio 《Journal of plant nutrition》2013,36(10):1613-1635
Aim of this work was to investigate if the variation among tomato genotypes in selenium (Se) uptake and accumulation observed in short term experiments are maintained over longer growth periods and if there is a positive correlation in shoot between sulphur (S) accumulation and Se accumulation across different genotypes or if higher tissue S results in greater feedback inhibition of Se uptake. Two experiments were carried out under greenhouse conditions and different genotypes of Lycopersicon lycopersicum (UC82B and LA2711), Lycopersicon pennellii (LA716) and Lycopersicon peruvianum (LA2157) were grown until fruit ripening. The results obtained in the two experiments confirmed that sulphate in the growth solution reduced selenate uptake by plants and increased the S content of the leaves. Under low sulphate treatment there was a clear correlation (R2=0.82) between leaf S content and shoot Se content across genotypes in both experiments, indicating that the overall activity of the S transport systems also determines Se transport. Selenium was translocated from shoot to fruit, but the edible portion of the plant contained much less total Se than the inedible plant parts. The difference in Se content between the low and the high sulphate treatments was significantly higher in shoot than in root, confirming that the Se translocation from root to shoot is probably more affected by high sulphate supply than Se uptake by root. In the first experiment the genotype LA716 showed ah higher Se, accumulation together with higher S content in leaves, indicating a marked ability of this genotype to absorb ions from substrate. In the second experiment UC82B appeared to be more capable to accumulate Se and S rather than LA2711 and LA2157. In both experiments Lycopersicon peruvianum appeared to be less affected by the high concentration of ions in the growth solution and to be able to reduce ion uptake than Lycopersicon lycopersicum and Lycopersicon pennellii. 相似文献
39.
Beatrice Grafl Dieter Liebhart Ayse Günes Patricia Wernsdorf Franz Aigner Josef Bachmeier Michael Hess 《Veterinary research》2013,44(1):38
In the present study day-old specific-pathogen-free (SPF) and commercial broilers with maternally derived fowl adenovirus serotype 1 (FAdV-1) antibodies were orally infected with a European “pathogenic” FAdV-1, isolated from broilers showing signs of gizzard erosion. During the experiment, broilers were observed and weighed daily up to 17 days post infection (dpi). Clinically, both infected groups showed significant decrease of weight compared to respective negative control groups. Birds were examined by necropsy at 3, 7, 10, 14 and 17 dpi. Pathological changes in the gizzards were noticed in both experimentally infected groups from 7 dpi onwards. Macroscopically, erosion of the koilin layer and inflammation or ulceration of the gizzard mucosa were observed. Histologically, presence of FAdV-1 in intranuclear inclusion bodies of degenerated glandular epithelial cells was demonstrated by in-situ hybridization and inflammatory cell infiltration of the lamina propria, submucosa and muscle layer was detected. Tissue samples were investigated by a recently developed real-time PCR and the viral DNA load was calculated from gizzard, liver, spleen and cloacal swabs with the highest amounts of FAdV-1 DNA found in the gizzard. For the first time, successful reproduction of clinical signs in broilers as well as pathological lesions in the gizzard were achieved with a European FAdV-1 isolate displaying some genetic differences to so far reported virulent FAdV-1 from Japan. Furthermore, highest viral load in gizzards could be linked with macroscopical and histological lesions. Therefore, the conducted analyses provide important insights into the pathogenesis of adenoviral gizzard erosion. 相似文献
40.
Barbro Ulén Stefan Pietrzak Beatrice Ramnerö Line Strand 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2016,66(4):359-368
Poland has the largest agricultural area within the Baltic Sea drainage basin and reducing the risk of phosphorus (P) and nitrogen (N) leaching from Polish soils to water is therefore essential. Increased acidity is known to reduce soil fertility and may trigger P leaching from non-calcareous soils. As part of advisor training, 25 farms each in Pomerania and north-western Mazovia were visited and 1500?ha arable soil, including 180?ha grassland soil, were monitored in 2013–2014. The soil was mainly coarse textured, but 25% of the Pomeranian farms were dominated by silty or clayey soils. More or less regular drainage systems were found on 20% of the farms, while 50% had simpler, older (>30 years) systems with a few single pipes. The farmers often used only ammonium sulphate or other acidifying N mineral fertiliser. Median pH on the Pomeranian farms, analysed in potassium chloride solution [pH(KCl)], was 5.2 and liming was advised for fields on most (72%) of these farms. Soil P content, measured by double-lactate extraction (PDL), was positively and significantly correlated (Pearson coefficient 0.57; p?.001) with soil pH and was generally higher for pig farms. Mean PDL (P given in elemental form) tended to be lower on dairy farms and arable farms and was significantly lower (mean 51?mg?PDL?kg?1 soil) on mixed farms (with just a few cows and poultry) than on pig farms (mean 122?mg?PDL?kg?1 soil). Farm-gate balances indicated deficits of P and potassium (K) on many of the small mixed farms in Pomerania and the soil can be expected to be nutrient depleted. In contrast, the pig farms demonstrated surplus farm-gate P balances (mean 27?kg?ha?1). The P leaching risk is discussed relative to soil threshold values and to results from Swedish long-term field experiments. 相似文献