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排序方式: 共有65条查询结果,搜索用时 15 毫秒
61.
Bastian SF 《Journal of the American Veterinary Medical Association》2004,225(1):31-2; author reply 32
62.
Kwon OD Yim CY Jeong KS Park SC Jung KY McGregor JR Bastian NR Samlowski WE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):357-365
Nitric oxide (NO*) synthesis is induced within many tumors. The time course of NO* synthesis was evaluated during intra-peritoneal Meth A fibrosarcoma progression. While increasing macrophage recruitment into ascites was noted, inducible nitric oxide synthase (iNOS) antigen and function peaked between days 3-6 after tumor implantation. The capacity of cells to respond to LPS and IFNgamma stimulation was markedly depressed on day 9 and 11. Cellular proliferation correlated in an inverse fashion with levels of NO* synthesis. Electron paramagnetic resonance spectroscopy and nitrotyrosine immunostaining failed to show accumulation of characteristic target cell lesions induced by NO*. These findings lead us to conclude that NO* production was increasingly suppressed during Meth A tumor progression. Depression of NO* production did not correlate with levels of the inhibitory cytokines TGFbeta and IL-10, but could be partially overcome by addition of sepiapterin (a tetrahydrobiopterin prodrug). Thus, depletion of essential co-factors necessary for iNOS function may contribute to depressed NO* responses during cancer progression. 相似文献
63.
J Cabezas E Lara P Pacha D Rojas D Veraguas F Saravia L Rodríguez‐Alvarez FO Castro 《Reproduction in domestic animals》2014,49(4):550-559
Endometrial stem cells have been identified in humans, mice and pigs. This study was designed to determine whether the uterine endometrium of cycling cows contains such cells, to identify markers of stemness and ultimately to isolate putative stem/progenitor cell and evaluate their capability to differentiate into mesodermal derivatives. Uteri from healthy cows in the early (days 1–5) and late luteal phases (days 13–18) of the oestrous cycle were collected. Total RNA and proteins were isolated and searched for gene markers of embryonic (OCT4, NANOG, SOX2) and mesenchymal (CD44, STAT3, CD‐117) stem cells and for protein markers (Oct4, Sox2, Cd44) in Western blots or immunostaining of paraffin‐embedded tissue. Primary cell cultures were isolated; characterized in terms of morphology, colony formation and gene/protein expression; and induced osteogenic and chondrogenic differentiation. We identified expression of embryonic (OCT4 and SOX2, but not NANOG) and mesenchymal (STAT3, CD44 and c‐KIT) gene markers in the endometrium of cycling cows and the encoded proteins (Oct4, Sox2 and Cd44) in both stages of the oestrous cycle. Derived cell lines displayed essentially the same gene expression pattern; however, at the protein level, Oct4 was not detected. No clear influence of the stage of the oestrous cycle was found. Cell lines from late luteal phase displayed osteogenic and chondrogenic differentiation potential upon chemical stimulation. In this research, we demonstrated the presence of mesenchymal progenitor cell populations of apparently mesenchymal origin in the endometrium of cycling cows, in both the early and late phases of the oestrous cycle. The cells isolated from the late luteal phase were more acquiescent to differentiate into mesodermal derivatives than cells in the early luteal phase. Our findings might have implications for the understanding of uterine stem cell biology in cows and other farm animal species. 相似文献
64.
Landscape services: the concept and its practical relevance 总被引:1,自引:1,他引:0
Olaf Bastian Karsten Grunewald Ralf-Uwe Syrbe Ulrich Walz Wolfgang Wende 《Landscape Ecology》2014,29(9):1463-1479
Recently, in addition to the popular concept of “ecosystem services” (ES), the term “landscape services” (LS) has come into use. We are examining the question of whether a stronger focus on LS would be useful, particularly with regard to case studies carried out in Germany. Important reasons for introducing the term LS include the prominent role of spatial aspects, the reference to landscape elements and the landscape character, and the relevance of LS for landscape planning. We found no strong arguments for replacing the concept of ES by LS; however, we do prefer a situation-related use of both concepts. We propose the following definition: Landscape services are the contributions of landscapes and landscape elements to human well-being. 相似文献
65.
Midje DL Bastian ED Morris HA Martin FB Bridgeman T Vickers ZM 《Journal of agricultural and food chemistry》2000,48(5):1630-1636
Mild cheese flavor in reduced fat Cheddar cheese was enhanced by using an integrated starter culture system. Three cultures, Lactococcus lactis subsp. cremoris SK11, L. lactis subsp. lactis biovar. diacetylactis JVI, and Lactobacillus casei 7A, were carefully selected to obtain a nonbitter, mildly acid, buttery flavored cheese. Cheeses were produced from all possible combinations of these cultures with the constraint that L. lactis subsp. cremoris SK11 was used as the primary acid-producing culture. Cheeses made with SK11 were compared to cheeses produced using an L. lactis subsp. cremoris commercial starter culture. Cheeses were ripened for 150 days and periodically sampled for chemical, microbiological, and sensory analysis. Cheeses produced with L. lactis subsp. cremorisSK11 had substantially lower bitterness intensity than the cheeses produced with commercial starter culture. L. lactis subsp. lactis biovar. diacetylactis JVI significantly increased diacetylacetoin and acetate concentrations. Sensory results indicate that these cheeses had increased buttery (diacetyl) flavor. 相似文献