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31.
AD Mackay 《New Zealand veterinary journal》2013,61(6):281-288
Abstract AIM: To find evidence for localisation in the uterus, and fetal infection, of Leptospira spp. in farmed deer in the lower North Island of New Zealand during and shortly after the breeding season. METHODS: Between February and July 2008, 116 blood samples, 120 kidneys, 120 uteri and 27 fetuses were collected from 120 mixed-age hinds from lines from nine farms, at a deer slaughter premises. Serum samples were tested for antibodies against Leptospira borgpetersenii serovar Hardjo-bovis and Leptospira interrogans serovar Pomona, using the microscopic agglutination test (MAT). For both serovars, a titre of >1:48 was considered positive. Samples from kidneys, uteri and fetal tissue were subjected to bacterial culture, using Ellinghausen-McCullough-Johnson-Harris (EMJH) medium, and real-time PCR, using DNA gyrase subunit B gene primers. RESULTS: Thirty-four of 116 (29.3%) serum samples were positive for serovar Hardjo-bovis, and 13 (11.2%) for serovar Pomona. Seven of 120 kidneys were positive for serovar Hardjo-bovis by culture, and five of these, but no others, were positive by real-time PCR. Of 120 uteri, none was culture- or PCR-positive. None of 27 fetal samples was culture-positive but one was positive by real-time PCR. The dam of the PCR-positive fetus was culture-negative from the kidney, but had an MAT titre of 1:192 for Hardjo-bovis. CONCLUSIONS: Attempts to isolate Leptospira spp. from the genital tracts and early fetuses of farmed deer were unsuccessful. However, molecular evidence suggested fetal infection in one case. This finding justifies further study of the role of leptospires in the genital tract and fetus and its association with reproductive loss in farmed deer. 相似文献
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Carlos Alberto Scapim Cleso Antônio Patto Pacheco Antônio Teixeira do Amaral Júnior Rafael Augusto Vieira Ronald José Barth Pinto Thiago Vincenzi Conrado 《Euphytica》2010,174(2):209-218
In plant breeding, correlations between the statistics of stability and adaptability of popcorn cultivars are not yet well
understood. Therefore, the objectives of the present experiment was to investigate the correlations between sdi2 \sigma_{\rm di}^{2} and bi \beta_{\rm i} from Eberhart and Russell, ωi from Wricke,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} and
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} from Huehn, Pi from Lin and Binns and the rank-sum from Kang, and indicate the most reliable method for selecting popcorn cultivars. These
statistics were estimated by data of crop yield from 19 Brazilian genotypes under 21 environments and popping expansion under
16 environments. The ωi,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} ,
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} and sdi2 \sigma_{\rm di}^{2} were positively and significantly correlated indicating that just one in these five statistics is sufficient for selecting
stable genotypes although they were not correlated with the means of crop yield and popping expansion. The bi \beta_{\rm i} was negatively and significantly correlated with Pi for crop yield indicating that the most adaptable genotypes tend to have the lowest estimates of Pi. Although Pi was not correlated with ωi,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} ,
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} , or sdi2 \sigma_{\rm di}^{2} statistics, it displayed positive correlation with the Index 1 (crop yield and popping expansion +
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} rank) and Index 2 (crop yield and popping expansion + Wi) indicating that superior popcorn genotypes are also stable. Finally, both Pi and the rank-sum are useful statistics in breeding programmes where crop yield, popping expansion and stability are essential
traits for selecting genotypes. 相似文献
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为了探寻不同发育期朝鲜球坚蚧的有效防治新措施,根据朝鲜球坚蚧不同发育阶段的形态特征和生物学习性,对不同龄期的若虫采取刮皮涂干、树干注射和常规喷雾等措施进行防治.试验结果表明;在施药方法上,刮皮涂干和常规喷雾是最为适用的;在药效方面,在1龄若虫期喷雾施用的几种药剂中醋盐合剂对1龄若虫的喷洒防效达到了90%以上.通过施用脱叶剂处理叶片的方法来防治2龄若虫,试验所设3种浓度的脱叶剂均能间接起到防治作用,其效果均达到了95%以上.在3龄若虫期喷雾施用的几种药剂中95%酒精合剂的喷洒防效达到了90%以上.涂干防治3龄若虫期供试的几种药剂中,20%吡虫啉可溶性液剂1∶10的稀释液防效达到了90%以上. 相似文献
37.
Brito LF Barth AD Rawlings NC Wilde RE Crews DH Mir PS Kastelic JP 《Domestic animal endocrinology》2007,33(1):1-18
The objective of the present study was to characterize the effects of nutrition on circulating concentrations of metabolic hormones, gonadotropins, and testosterone during sexual development in bulls. Nutrition regulated the hypothalamus-pituitary-testes axis through effects on the GnRH pulse generator in the hypothalamus and through direct effects on the testes. Pituitary function (gonadotropin secretion after GnRH challenge) was not affected by nutrition. However, nutrition affected LH pulse frequency and basal LH concentration during the early gonadotropin rise (10-26 weeks of age). There were close temporal associations between changes in insulin-like growth factor-I (IGF-I) concentrations and changes in LH pulse frequency, suggesting a role for IGF-I in regulating the early gonadotropin rise in bulls. The peripubertal increase in testosterone concentration was delayed in bulls with lesser serum IGF-I concentrations (low nutrition), suggesting a role for IGF-I in regulating Leydig cell function. Serum IGF-I concentrations accounted for 72 and 67% of the variation in scrotal circumference and paired-testes volume, respectively (at any given age), indicating that IGF-I may regulate testicular growth. Bulls with a more sustained elevated LH pulse frequency during the early gonadotropin rise (high nutrition) had greater testicular mass at 70 weeks of age relative to the control group (medium nutrition), despite no differences in metabolic hormone concentrations after 26 weeks of age. Therefore, gonadotropin-independent mechanism regulating testicular growth might be dependent on previous gonadotropin milieu. 相似文献
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The effect of streptomycin, oxytetracycline, tilmicosin and phenylbutazone on spermatogenesis in bulls 下载免费PDF全文
Barth AD Wood MR 《The Canadian veterinary journal. La revue veterinaire canadienne》1998,39(2):103-106
To determine whether declining semen quality associated with health problems may be due to certain antibiotic or anti-inflammatory treatments, semen was collected 3 times per week for up to 42 d from 6 normal bulls after treatment with oxytetracycline, tilmicosin, dihydrostreptomycin, or phenylbutazone. No adverse effects on semen quality were observed. 相似文献
39.
Javier Puntieri Javier Grosfeld Marina Stecconi Cecilia Brion María Marta Azpilicueta Leonardo Gallo Daniel Barthélémy 《Annals of Forest Science》2007,64(8):839-844
Shoot growth and dieback were compared among progenies of nursery-grown seedlings of Nothofagus obliqua belonging to seven progenies of the same provenance (Quila-Quina, Argentina). First-year shoots consisted of one growth unit (GU) and second-year shoots of one or two GUs. The probability of development of two GU was similar for all progenies. Progenies were different in terms of shoot size, terminal bud abscission, the extent of shoot dieback after shoot extension and the node of origin of the relay shoot on the first shoot. Plants with a second-year shoot consisting of two GUs had a thicker stem and more nodes than those with single-GU shoots. The selection of N. obliqua seed trees based on architectural traits suitable for forestry development at specific sites must contemplate variability among progenies and their probabilities of successful development under different conditions. 相似文献
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