Peste des petits ruminants virus (PPRV) is a morbillivirus that can cause severe disease in sheep and goats, characterised by pyrexia, pneumo-enteritis, and gastritis. The socio-economic burden of the disease is increasing in underdeveloped countries, with poor livestock keepers being affected the most. Current vaccines consist of cell-culture attenuated strains of PPRV, which induce a similar antibody profile to that induced by natural infection. Generation of a vaccine that enables differentiation of infected from vaccinated animals (DIVA) would benefit PPR control and eradication programmes, particularly in the later stages of an eradication campaign and for countries where the disease is not endemic. In order to create a vaccine that would enable infected animals to be distinguished from vaccinated ones (DIVA vaccine), we have evaluated the immunogenicity of recombinant fowlpox (FP) and replication-defective recombinant human adenovirus 5 (Ad), expressing PPRV F and H proteins, in goats. The Ad constructs induced higher levels of virus-specific and neutralising antibodies, and primed greater numbers of CD8+ T cells than the FP-vectored vaccines. Importantly, a single dose of Ad-H, with or without the addition of Ad expressing ovine granulocyte macrophage colony-stimulating factor and/or ovine interleukin-2, not only induced strong antibody and cell-mediated immunity but also completely protected goats against challenge with virulent PPRV, 4 months after vaccination. Replication-defective Ad-H therefore offers the possibility of an effective DIVA vaccine. 相似文献
The aim of this work was to evaluate the efficiency of the cryoprotectants dimethylformamide and ethylene glycol for cryopreservation of ovine embryos using vitrification and conventional freezing. The recovered embryos were distributed randomly in three treatment groups: Gr. 1: conventional freezing (n = 44), Gr. 2: vitrification with ethylene glycol (n = 39) and Gr. 3: vitrification with dimethylformamide (n = 38). Quality of fresh embryos in control group as well as of frozen and vitrified embryos was examined by three methodologies: staining with propidium iodide and Hoechst 33258 and evaluation under fluorescent microscopy, evaluation of re‐expansion and hatching rates after culture, and determination of apoptotic index with TUNEL technique. It was established that re‐expansion rate in all treatment groups was similar. In the same time, hatching rates were higher in Gr. 1 (40.5%) and Gr. 2 (35.3%) in comparison with Gr. 3 (15.5%, p < 0.05). The number of dead cells in vitrified embryos of Gr. 2 and Gr. 3 was higher (42.6 ± 26.2 and 63.2 ± 34.65, respectively) in comparison with Gr. 1 (conventional freezing, 10.1 ± 8.5, p < 0.05). Embryos vitrified with dimethylformamide included the same quality of apoptotic cells that Gr. 1 (conventional freezing) and fresh embryos. In conclusion, the dimethylformamide and ethylene glycol used as cryoprotectant to vitrify ovine embryos, in the concentrations and exposition time tested in this work, were not as efficient as the conventional freezing for cryopreservation of ovine embryos Thus, the conventional freezing with ethylene glycol was the most efficient method to cryopreserve ovine embryos in comparison with vitrification. 相似文献
The spatial organization and time dependence of Jupiter's stratospheric temperatures have been measured by observing thermal emission from the 7.8-micrometer CH(4) band. These temperatures, observed through the greater part of a Jovian year, exhibit the influence of seasonal radiative forcing. Distinct bands of high temperature are located at the poles and mid-latitudes, while the equator alternates between warm and cold with a period of approximately 4 years. Substantial longitudinal variability is often observed within the warm mid-latitude bands, and occasionally elsewhere on the planet. This variability includes small, localized structures, as well as large-scale waves with wavelengths longer than approximately 30,000 kilometers. The amplitudes of the waves vary on a time scale of approximately 1 month; structures on a smaller scale may have lifetimes of only days. Waves observed in 1985, 1987, and 1988 propagated with group velocities less than +/-30 meters per second. 相似文献
Lysates of the protozoan parasite Entamoeba histolytica altered active electrolyte transport when present on the serosal surface of rabbit ileum and rat colon. The lysate-induced effects on electrolyte transport were similar to those caused by serotonin, and were blocked by bufotenine, an analog known to inhibit the action of serotonin. The transport effects were partially inhibited by antibody to serotonin. The amebic lysates were shown to contain serotonin by radioimmunoassay, high-performance liquid chromatography, and thin-layer chromatography. These results suggest that the serotonin present in Entamoeba histolytica may be important in the diarrhea seen in amebiasis. 相似文献
Water-binding properties of myofibrils extracted from porcine muscle, and added hemoglobin with and without exposure to H2O2, were characterized using low-field proton NMR T2 relaxometry. The effects of pH and ionic strength in the samples were investigated as pH was adjusted to 5.4, 6.2, and 7.0 and ionic strength was adjusted to 0.29, 0.46, and 0.71 M, respectively. The formation of dityrosine as a measure of oxidative protein cross-linking revealed a significant increase in dityrosine concentrations upon H2O2 activation. The formation of dityrosine was strongly pH-dependent and increased with decreasing pH. In addition, increased levels of thiobarbituric acid reactive substances were observed upon addition of H2O2, implying that lipid oxidation was enhanced, however, with a different oxidation pattern as compared to the myofibrillar proteins. Low-field NMR relaxation measurements revealed reduced T2 relaxation times upon H2O2 activation, which corresponds to reduced water-holding capacity upon oxidation. However, a direct relationship between degree of oxidation and T2 relaxation time was not observed with various pH values and ionic strengths, and further studies are needed for a complete understanding of the effect of oxidation on myofibrillar functionality. 相似文献
In fire-excluded forests across western North America, recent intense wildfire seasons starkly contrast with fire regimes of the past. The last 100 years mark a transition between pre-colonial and modern era fire regimes, providing crucial context for understanding future wildfire behavior.
Objectives
Using the greatest time depth of digitized fire events in Canada, we identify distinct phases of wildfire regimes from 1919 to 2019 by evaluating changes in mapped fire perimeters (>?20-ha) across the East Kootenay region (including the southern Rocky Mountain Trench), British Columbia.
Methods
We detect transitions in annual number of fires, burned area, and fire size; explore the role of lightning- and human-caused fires in driving these transitions; and quantify departures from historical fire frequency at the regional level.
Results
Relative to historical fire frequency, fire exclusion has created a significant fire deficit in active fire regimes, with a minimum of 1–10 fires missed across 46.4-percent of the landscape. Fire was active from 1919 to 1939 with frequent and large fire events, but the regime was already altered by a century of colonization. Fire activity decreased in 1940, coinciding with effective fire suppression influenced by a mild climatic period. In 2003, the combined effects of fire exclusion and accelerated climate change fueled a shift in fire regimes of various forest types, with increases in area burned and mean fire size driven by lightning.
Conclusions
The extent of fire regime disruption warrants significant management and policy attention to alter the current trajectory and facilitate better co-existence with wildfire throughout this century.
