全文获取类型
收费全文 | 1649篇 |
免费 | 82篇 |
国内免费 | 2篇 |
专业分类
林业 | 129篇 |
农学 | 27篇 |
基础科学 | 4篇 |
274篇 | |
综合类 | 177篇 |
农作物 | 87篇 |
水产渔业 | 87篇 |
畜牧兽医 | 804篇 |
园艺 | 33篇 |
植物保护 | 111篇 |
出版年
2023年 | 17篇 |
2022年 | 9篇 |
2021年 | 26篇 |
2020年 | 39篇 |
2019年 | 46篇 |
2018年 | 37篇 |
2017年 | 39篇 |
2016年 | 46篇 |
2015年 | 41篇 |
2014年 | 47篇 |
2013年 | 81篇 |
2012年 | 105篇 |
2011年 | 139篇 |
2010年 | 42篇 |
2009年 | 71篇 |
2008年 | 108篇 |
2007年 | 114篇 |
2006年 | 90篇 |
2005年 | 96篇 |
2004年 | 86篇 |
2003年 | 87篇 |
2002年 | 65篇 |
2001年 | 18篇 |
2000年 | 21篇 |
1999年 | 20篇 |
1998年 | 16篇 |
1997年 | 19篇 |
1996年 | 13篇 |
1995年 | 8篇 |
1994年 | 9篇 |
1993年 | 9篇 |
1992年 | 12篇 |
1991年 | 8篇 |
1990年 | 10篇 |
1989年 | 8篇 |
1988年 | 6篇 |
1987年 | 7篇 |
1986年 | 4篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 10篇 |
1982年 | 6篇 |
1980年 | 4篇 |
1979年 | 9篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1975年 | 18篇 |
1974年 | 4篇 |
1970年 | 4篇 |
排序方式: 共有1733条查询结果,搜索用时 15 毫秒
991.
992.
Eva-Maria Laabs Reinhard Mischke Peter Dziallas Arianna Maiolini Andrea Tipold Barbara Raddatz Christina Puff Jürgen Rehage 《Acta veterinaria Scandinavica》2015,57(1)
A three-month-old female Holstein–Friesian calf was presented with acute tetraparesis. After neurological examination a multifocal lesion in the central nervous system was suspected with the most pronounced lesions between the third thoracic and the third lumbar vertebrae. Haematological examination revealed moderate anaemia as well as severe thrombocytopenia, neutropenia and leucocytosis. A blood smear and bone marrow aspirate exhibited predominantly blasts with basophilic granulation leading to a diagnosis of acute (myeloid) leukaemia with involvement of the basophilic lineage or an acute basophilic leukaemia. Magnetic resonance imaging revealed spinal cord compression; at necropsy, extensive localised haemorrhages extending into the thoracic vertebral canal were found. Histopathology revealed a large population of blast cells in several tissues including the meninges. Due to multifocal detection of neoplastic cells in the vascular system, neoplasia of the haematopoietic system was assumed in agreement with haematological findings. Signs of paresis could be explained by intramedullary spinal cord haemorrhage and myeloid infiltrations of meningeal vessels. In conclusion, despite its rarity, acute myeloid leukaemia with involvement of the basophilic lineage may be considered in diagnosing calves with progressive deteriorating general condition, paresis, leucocytosis with moderate basophilic differentiation or haemorrhagic disorders. 相似文献
993.
Ross J Dallap BL Dolente BA Sweeney RW 《American journal of veterinary research》2007,68(9):1016-1021
OBJECTIVE: To determine the pharmacokinetics and pharmacodynamics of epsilon-aminocaproic acid (EACA), including the effects of EACA on coagulation and fibrinolysis in healthy horses. ANIMALS: 6 adult horses. PROCEDURES: Each horse received 3.5 mg of EACA/kg/min for 20 minutes, i.v. Plasma EACA concentration was measured before (time 0), during, and after infusion. Coagulation variables and plasma alpha(2)-antiplasmin activity were evaluated at time 0 and 4 hours after infusion; viscoelastic properties of clot formation were assessed at time 0 and 0.5, 1, and 4 hours after infusion. Plasma concentration versus time data were evaluated by use of a pharmacokinetic analysis computer program. RESULTS: Drug disposition was best described by a 2-compartment model with a rapid distribution phase, an elimination half-life of 2.3 hours, and mean residence time of 2.5 +/- 0.5 hours. Peak plasma EACA concentration was 462.9 +/- 70.1 microg/mL; after the end of the infusion, EACA concentration remained greater than the proposed therapeutic concentration (130 microg/mL) for 1 hour. Compared with findings at 0 minutes, EACA administration resulted in no significant change in plasma alpha(2)-antiplasmin activity at 1 or 4 hours after infusion. Thirty minutes after infusion, platelet function was significantly different from that at time 0 and 1 and 4 hours after infusion. The continuous rate infusion that would maintain proposed therapeutic plasma concentrations of EACA was predicted (ie, 3.5 mg/kg/min for 15 minutes, then 0.25 mg/kg/min). CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that EACA has potential clinical use in horses for which improved clot maintenance is desired. 相似文献
994.
Much remains to be discovered about the population genetic structure of parasites, despite the importance of such knowledge to understanding the processes involved in the spread of drug resistance through populations. Here we present a study of population genetic diversity in Trichostrongylus tenuis, an avian parasitic nematode infecting both poultry and game birds, where anthelmintic use is common. We examined diversity of nicotinamide dehydrogenase subunit 4 (nad4) mtDNA sequences within and between seven locations: five in the UK (red grouse hosts), one in Iceland (domestic goose) and one in Norway (willow grouse). Within-UK comparisons showed high nucleotide diversity (pi=0.015, n=23) but no structure between locations (Phi(ST)=0.022, P=0.27), with over 97% of variation distributed within-hosts. The highest diversity was found in Iceland (pi=0.043, n=4), and the lowest in Norway (pi=0.003, n=4). Differentiation between countries was considerable (Phi(CT)=0.44, P<0.05), in spite of the potential mixing effects of gene flow via migrating wild hosts and the poultry trade. However, significant pairwise F(ST) values were found only between Norway and the other locations. Phylogenetic analysis provided statistical support for a separate clade for Norwegian samples only, with unresolved diversity leading to a star-shaped relationship between Icelandic and UK haplotypes. These results suggest that Norwegian T. tenuis are isolated, but that there is some connectivity between UK and Icelandic populations. Although anthelmintic resistance has not yet been reported for T. tenuis, the population structure is such that emerging resistance has the potential to spread by gene flow over a large geographic scale. 相似文献
995.
Schmerbach KI Boeltzig CK Reif U Wieser JC Keller T Grevel V 《Veterinary surgery : VS》2007,36(2):156-163
OBJECTIVE: To evaluate the influence of a tibial plateau leveling jig on osteotomy orientation, fragment reduction, and postoperative tibial plateau angle (TPA) during tibial plateau leveling osteotomy (TPLO). STUDY DESIGN: In vitro experimental study. ANIMALS: Large-breed canine cadavers (n=20). METHODS: TPLO was performed on 40 hindlimbs using 4 methods. Group 1: Jig; dogs in dorsal recumbency with the osteotomy parallel to the distal jig pin. Groups 2-4: No jig; dogs in lateral recumbency with the osteotomy in a vertical orientation (group 2: tibia parallel to the table top; group 3: controlled superimposition of the femoral condyles; group 4: internal rotation of the tibia). Postoperative TPA, fragment reduction, and osteotomy orientation relative to the tibial plateau were compared. Positive or negative values denoted deviation from parallel relative to the tibial plateau. RESULTS: Postoperative TPA, fragment reduction, and proximodistal osteotomy orientation were not significantly different between groups. Craniocaudal osteotomy orientation was significantly different (P<.005) from the tibial plateau. Median deviations were -4.0 degrees (group 1), 11.8 degrees (group 2), 11.2 degrees (group 3), and 0.2 degrees (group 4). Group 1 was not significantly different from group 4. CONCLUSIONS: A jig is not essential for osteotomy orientation, tibial plateau rotation, or fragment reduction. Comparable results were achieved performing a vertical osteotomy with the tibia slightly internally rotated (10 degrees -15 degrees) and parallel to the table surface. CLINICAL RELEVANCE: TPLO without use of a jig reduces surgical trauma, is less time consuming, and reduces cost. 相似文献
996.
The effect of plant-derived, nonsteroidal estrogens (genistein, daidzein and biochanin A) on cortisol and androstenedione secretion by porcine adrenocortical cells was examined. Adrenals were harvested from locally slaughtered mature gilts on days 5-9 of the oestrous cycle. Adrenocortical cells were incubated with or without genistein, daidzein, biochanin A (0.5, 5, 10 or 50 microM), oestradiol (10, 50, 100 or 500 pg/mL) and ACTH (5 nM--positive control). All incubations were performed for 8 h (95% air and 5% CO2, 37 degrees C). ACTH increased cortisol and androstenedione secretion. Genistein, daidzein and biochanin A suppressed cortisol output, whereas androstenedione secretion was enhanced by these phytoestrogens. In contrast, oestradiol did not alter steroid secretion by porcine adrenocortical cells, which may suggest that phytoestrogens have a non-oestrogenic mechanism of action. Therefore, phytoestrogens present in commercial forage may influence adrenocortical function in pigs by decreasing cortisol and increasing androstenedione secretion. 相似文献
997.
Moore AS Dernell WS Ogilvie GK Kristal O Elmslie R Kitchell B Susaneck S Rosenthal R Klein MK Obradovich J Legendre A Haddad T Hahn K Powers BE Warren D 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(4):783-790
BACKGROUND: This study was designed to assess the efficacy of a matrix metalloproteinase inhibitor in prolonging posttreatment survival for dogs with appendicular osteosarcoma after treatment with amputation and doxorubicin chemotherapy. HYPOTHESIS: Survival will be prolonged in dogs receiving BAY 12-9566. ANIMALS: The study included 303 dogs with appendicular osteosarcoma. METHODS: Dogs were treated with doxorubicin (30 mg/m2) every 2 weeks for 5 treatments starting 2 weeks after amputation. Dogs were randomly allocated to receive a novel nonpeptidic biphenyl inhibitor of matrix metalloproteinases (MMPs, BAY 12-9566; 4-[4-4-(chlorophenyl)phenyl]-4-oxo-2S-(phenylthiomethyl) butanoic acid) or placebo after doxorubicin chemotherapy. RESULTS: Median survival for all 303 dogs was 8 months; and 1-year, 2-year, and 3-year survival rates were 35%, 17%, and 9%, respectively. Treatment with BAY 12-9566 did not influence survival. Multivariate analysis revealed that increasing age (P = .004), increasing weight (P = .006), high serum alkaline phosphatase (ALP) (P = .012) and high bone ALP (P < .001) were independently associated with shorter median survival times. Additional analyses on available data indicated that as the number of mitotic figures in the biopsy increased (P = .013), and as plasma active MMP-2 concentrations increased (P = .027), the risk of dying increased. CONCLUSIONS AND CLINICAL IMPORTANCE: Doxorubicin is an effective adjuvant to amputation in prolonging survival for dogs with appendicular osteosarcoma. 相似文献
998.
U'Ren LW Biller BJ Elmslie RE Thamm DH Dow SW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(1):113-120
BACKGROUND: Hemangiosarcoma (HSA) is a highly metastatic and often rapidly fatal tumor of dogs. At present, adjuvant chemotherapy is the only proven effective treatment for dogs with HSA, though the benefits from chemotherapy are modest. Administration of immunotherapy together with chemotherapy has also been reported to improve survival in dogs with HSA. Therefore, we evaluated safety and immunologic responses to a novel tumor vaccine administered together with doxorubicin chemotherapy in dogs with different stages of HSA. HYPOTHESIS: That tumor vaccination could be safely and effectively combined with doxorubicin chemotherapy for treatment of dogs with HSA. ANIMALS: Twenty-eight dogs with various stages of HSA were enrolled in the study. METHODS: The HSA vaccine was prepared with lysates of allogeneic canine HSA cell lines mixed with an adjuvant composed of liposome-DNA complexes. Dogs received a series of 8 immunizations administered over a 22-week period, and most also received chemotherapy. Clinical adverse effects were noted, immune responses were measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry, and survival times were calculated. RESULTS: The most common adverse effects observed in vaccinated dogs also treated with doxorubicin chemotherapy were diarrhea and anorexia. Vaccinated dogs were found to mount strong humoral immune responses against a control antigen and, most dogs also mounted antibody responses against canine HSA cells. Thirteen dogs with stage II splenic HSA that received the tumor vaccine plus doxorubicin chemotherapy had an overall median survival time of 182 days. CONCLUSIONS: We conclude that an allogeneic tumor lysate vaccine is safe in dogs with HSA and can elicit humoral immune responses in dogs that are receiving concurrent doxorubicin chemotherapy. 相似文献
999.
Belowground interactions for water between trees and grasses in a temperate semiarid agroforestry system 总被引:1,自引:0,他引:1
María Elena Fernández Javier Gyenge Julian Licata Tomás Schlichter Barbara J. Bond 《Agroforestry Systems》2008,74(2):185-197
A fundamental hypothesis of agroforestry is the complementary use of soil resources. However, productivity of many agroforestry
systems has been lower than expected due to net competition for water, highlighting the need for a mechanistic understanding
of belowground interactions. The goal of this study was to examine root–root interactions for water in a temperate semiarid
agroforestry system, based on ponderosa pines and a Patagonian grass. The hypotheses were: (a) A greater proportion of water
uptake by pines is from deeper soil layers when they are growing with grasses than when they are growing alone; (b) Growth
of grasses is improved by the use of water hydraulically lifted by pines. We used stable isotopes of O to analyze water sources
of plants, and we measured sapflow direction in pine roots and continuous soil water content with a very sensitive system.
We also installed barriers to isolate the roots of a set of grasses from pine roots, in which we measured water status, relative
growth and water sources, comparing to control plants. The results indicated that pines and grasses show some complementary
in the use of soil water, and that pines in agroforestry systems use less shallow water than pines in monoculture. We found
evidence of hydraulic lift, but contradicting results were obtained comparing growth and isotope results of the root isolation
experiment. Therefore, we could not reject nor accept that grasses use water that is hydraulically lifted by the pines, or
that this results in a positive effect on grass growth. This information may contribute to understand the complex and variable
belowground interactions in temperate agroforestry. 相似文献
1000.
Julian M. Norghauer Jay R. Malcolm Barbara L. Zimmerman Jeanine M. Felfili 《Forest Ecology and Management》2008
Research into the mechanisms underlying edaphic associations reported for many tropical forest tree species, including those in the Meliaceae, has paid little attention to the earliest post-dispersal life stages. We conducted a reciprocal sowing experiment to examine germination and establishment rates of big-leaf mahogany (Swietenia macrophylla King) during the first seedling growing season in two local edaphic habitats in southeastern Amazonia. In this region, adult populations are restricted primarily to low-ground soils associated with seasonal streams, but rarely are found on high-ground soils. Soil analyses revealed that low-ground soils at the study site were silty loams whereas high-ground soils were clays. High-ground soils were less acidic and more fertile than low-ground soils, especially with respect to total nitrogen, Mg, and sum of basic cations. 相似文献