金钱豹血液理化值的测定

广州动物园科研中心于2003年10月至2005年3月间共采集11只金钱豹的34份血液样本，对40项临床常用的血液生理生化值进行了测定，结果用SPSS11．0统计软件进行统计分析。     

生长前期北京鸭维生素E需要量的研究

试验采用单因子完全随机试验设计,研究不同水平维生素E对北京鸭生产性能的影响,进而探讨0～14日龄北京鸭维生素E的需要量。试验一维生素E水平为0、5、10、20、40、100IU/kg和400IU/kg,选取392只1日龄健康雄性北京鸭随机分为7个处理组,每处理组8个重复,每重复7只。试验二采用半纯合日粮,维生素E水平为0、10、20IU/kg和100IU/kg,选取192只1日龄健康雄性北京鸭随机分为4个处理组,每处理组6个重复,每重复8只。饲养期均为14d。试验结果表明:维生素E可提高0～14日龄北京鸭生产性能,以日增重为评价指标,北京鸭维生素E最低需要量为10IU/kg。     

魔芋软腐病侵染规律试验研究初报

魔芋生产系陕西省安康地区农民增收的重要项目。但是,随着种植年限的增加,软腐病逐年加重,严重地影响魔芋生产。由于魔芋病害研究工作开展较少,迄今魔芋软腐病的侵染循环规律尚未见报道。为了探索魔芋软腐病的侵染规律,有效指导大田防治,开展了此项研究。１　试验设计与方法试验设３个处理。①切芋播种前在软腐病菌液中浸种１ｍｉｎ;②整芋播种前在菌液里浸种１ｍｉｎ;③整芋消毒后播种作对照。种芋消毒方法为播种前１ｄ用５０％甲基托布津可湿性粉剂６００倍液浸种３０ｍｉｎ后捞出晾干。每小区播种３０窝（芋种单个重量约５０ｇ,…     

湖南省农产品加工业发展现状及对策

农产品加工业是国民经济的重要支柱产业,具有广阔的发展前景.本文详细介绍了湖南省农产品加工业的发展现状,分析了制约湖南农产品加工业发展的主要因素,并就湖南省农产品加工业的发展思路、发展重点及对策措施等提出了意见和建议.     

Structural characterization of lignin in the process of cooking of cornstalk with solid alkali and active oxygen

A novel, efficient, and environmentally friendly technology is used in cornstalk cooking, active oxygen (O? and H?O?) cooking with solid alkali (MgO). After the cooking, the milled wood lignin in the raw material and pulp and the water-soluble and insoluble lignin in the yellow liquor were all characterized by attenuated total reflectance Fourier transform infrared spectroscopy and two-dimensional heteronuclear single-quantum coherence NMR. The results showed that the cooking procedure with solid alkali and active oxygen had a high selectivity for delignification, which could remove 85.5% of the lignin from the raw material. The syringyl (S/S'/S') units could be dissolved preferentially because of their high reactivity, and a novel guaiacyl unit with a carbonyl group (G') was generated in the cooking process. Moreover, during the cooking, the β-O-4' (A/A'/A″) structures as the main side-chain linkages in all the lignins could be partly broken and the β-O-4' (A') with a ring-conjugated structure was readily attacked by oxygen, whereas the H unit and β-5' and β-β' structures were found to stay stable without characteristic reaction.     

衣原体单克隆抗体在规模化牛羊场相关抗体的检测中的应用

采用淋巴细胞杂交瘤技术,研制出的抗衣原体单克隆抗体(McAb),应用酶联免疫吸附试验(ELISA)方法,对本地区所采的1361份牛血清,698份羊血清进行检测,结果:牛血清阳性检出率为9.18%,羊为22.06%。选择牛羊血清各50份用已建立的检测衣原体抗体ELISA方法与传统的间接血凝试验(IHA)进行比较,其敏感性高。牛血清ELISA阳性检出率为32%,IHA为24%,ELISA比IHA试验高出8个百分点。羊血清ELISA阳性检出率为26%,IHA为22%,前者比IHA高出4个百分点。     

Design and Synthesis of Novel Phenylahistin Derivatives Based on Co-Crystal Structures as Potent Microtubule Inhibitors for Anti-Cancer Therapy

Phenylahistin is a naturally occurring marine product with a diketopiperazine structure that can bind to the colchicine site of microtubulin as a possible anticancer agent. To develop more potent microtubule inhibitors, novel phenylahistin derivatives were designed and synthesized based on the co-crystal complexes of phenylahistin derivatives and microtubulin. We established a focused library of imidazole-type molecules for the introduction of different groups to the C-ring and A-ring of phenylahistin. Structure–activity relationship studies indicated that appropriate hydrocarbon substituents and unsaturated alkenyl substituents at the 1-position of the imidazole group are important for improving the activity of such compounds. In addition, this study found that propylamine groups could maintain the activity of these compounds, as exemplified by compound 16d (IC₅₀ = 5.38 nM, NCI-H460). Compound 15p (IC₅₀ = 1.03 nM, NCI-H460) with an allyl group exhibited potent cytotoxic activity at the nanomolar level against human lung cancer cell lines. Immunofluorescence assay indicated that compound 15p could efficiently inhibited microtubule polymerization and induced a high expression of caspase-3. 15p also displayed good pharmacokinetic characteristics in vitro. Additionally, the growth of H22 transplanted tumors was significantly inhibited in BALB/c mice when 15p alone was administered at 4 mg/kg, and the tumor inhibition rate was as much as 65%. Importantly, the continuous administration of 15p resulted in a lower toxicity than that of docetaxel (10 mg/kg) and cyclophosphamide (20 mg/kg). Overall, the novel allyl-imidazole-diketopiperazine-type derivatives could be considered safe and effective potential agents for cancer treatment.     

广东省东莞市2010—2014年猪伪狂犬病野毒抗体监测与分析

利用ELISA方法对猪伪狂犬病野毒（gE）抗体进行检测,分析2010—2014年连续5年来自东莞32个镇街820个养猪场点12 108份血清的检测结果。分析发现：2010—2012年,东莞猪伪狂犬病野毒感染程度整体呈下降趋势,2012年阳性率最低（8.37%）,2013年开始反弹,2014年达到46.97%;每年各季度均能检出伪狂犬病毒gE抗体,其中2014年第二季度的检出率最高,但在第四季度有所回落。分析认为,2013年后东莞市猪伪狂犬病野毒抗体阳性率上升的原因可能是出现了新变异毒株;定期监测猪群的伪狂犬病毒gE抗体水平,尤其是种猪,及时淘汰阳性猪,仍是控制猪伪狂犬病的重要手段。