盐胁迫下粪产碱菌在水稻根表的定殖

粪产碱菌A1 5 0 1能在氯化钠浓度高达 4%的固体和液体介质中良好生长 ,合成IAA和固定空气中的氮素。当氯化钠浓度超过 3 %时 ,A1 5 0 1丧失运动和趋化能力。一定盐浓度胁迫下A1 5 0 1能定殖在水稻根表和根毛区和侧根伸出部位 ,表现出较高的联合固氮活性。0 5 %的氯化钠能促进A1 5 0 1在水稻根表的定殖能力。A1 5 0 1能通过侧根伸出部位侵入根内。     

Preliminary Studies on Cryopreservation of Common Tench (Tinca tinca) Embryos (Work in Progress)

Vitrification could provide a promising tool for the cryopreservation of fish embryos. However, to achieve cryopreservation using vitrification, chilling sensitivity and cryoprotectants toxicity were determined using tench embryos at four developmental stages (11, 17, 23 and 29 h). Embryos treated with alcalase (2 ml/998 ml, 2 min at 22°C) were exposed to chilling with/without warming. Other embryos were exposed to methanol and glycerol at the concentration of 10% and 20% for periods of 20 min. At last, embryos were incubated at special incubator cages where hatching rates were counted. Regarding chilling sensitivity and exposure to chilling followed by warming, the hatching rates of embryos decreased significantly (p < 0.001) after exposure to 0°C at all developmental stages except the 29-h stage compared with the controls. The embryo stage most sensitive to chilling was 11-h stage. The 29-h stage exhibited the least sensitivity to low temperature while 17-h and 23-h stages were intermediate in their sensitivity to chilling. The toxicity of methanol increased significantly (p < 0.001) with developmental stage for 11, 17 and 23-h stages. The highest hatching rates of tench embryos were obtained with 29-h embryos using various concentrations of methanol. The hatching rates of tench embryos exposed to glycerol concentrations were approximately similar to those embryos exposed to methanol concentrations except for 11-h embryos that showed no hatching. Unfortunately, we could not obtain living embryos in any of the conditions examined after vitrification. In conclusion, it was quite difficult to vitrify the tench embryos during this study using various vitrifying solutions and the method reported by Chen & Tian (2005) and further studies are needed to achieve successful cryopreservation.     

Peripheral blood lymphocyte subsets in fleece rot-resistant and -susceptible sheep

Objective To compare haematological values and lymphocyte phenotypes in the peripheral blood of fleece rot-resistant and -susceptible sheep.
Procedure Experiments were conducted on 2- and 3-year-old Merino rams, flock 1 (17 rams) and flock 2 (32 rams), respectively. Within each flock, individual rams were classified as fleece rot-resistant or -susceptible, based on established criteria. Total and differential white cell counts, and indirect fluorescent antibody tests specific for B cells and T cells were performed on all sheep. The concentration of various subsets of circulating lymphocytes was then determined in each sheep.
Results There were no significant differences between fleece rot-resistant and -susceptible sheep from either flock in the mean total or differential white cell counts. However, fleece rot-resistant rams in flock 1 did have a significantly higher concentration of circulating SBU-T1⁺ cells than fleece rot-susceptible rams from the same flock. No such difference was noted in the rams from flock 2. While all rams in flock 1 were free of clinical fleece rot, 24 rams in flock 2 (comprising all 17 fleece rot-susceptible and 7 of 15 fleece rot-resistant animals) had clinical signs of the disease. Fleece rot-free rams in this flock (irrespective of their classification as fleece rot-resistant or -susceptible) had significantly higher concentrations of circulating SBU-T1⁺ cells compared with fleece rot-affected animals. They also had significantly higher concentrations of circulating B cells, and total lymphocytes.
Conclusions An examination of peripheral blood lymphocyte subsets in fleece rot-resistant and -susceptible sheep revealed a possible association between resistance to fleece rot and the concentration of circulating SBU-T1⁺ cells.     

Sodium, potassium and calcium concentrations in varieties of Dactylis glomerata

Twelve varieties of cocksfoot, Dactylis glomerata L., were analysed for three mineral elements (Na, K, Ca) and their dry matter (DM) yields were measured in pot and field trials. Among these characteristics there were strong varietal differences in Na concentration, whereas in other cases the varietal differences were less significant. The concentrations of individual mineral elements were, in most cases, independent of yield and of one another.
Parent plants and their clonal derivatives were highly correlated in Na and Ca concentration as well as in DM yield. Tiller size or position on the parental plant had no significant effects on the mineral concentrations or DM yields of clones derived from separated tillers.
Within varieties continuous variation was observed for Na concentration and the range of variation increased with increasing mean Na concentration. When plants were grown under different experimental conditions there was a high repeatability in Na concentration within varieties over cuts and years. This result was taken as an indication of a strong genetic basis for Na concentration. The results obtained in this study show that it is possible to develop grass varieties of defined potential Na concentration.