The application of ultraviolet microscopy to the distribution of lignin in wood description and validity of the technique

A method has been developed for the determination of lignin distribution in the wood cell wall by ultraviolet microscopy. The method incorporates some important advances on previus applications of UV microscopy to the study of lignin distribution. Ultrathin cross-sections of wood are obtained by the sample preparation and sectioning techniques of electron microscopy. The specimens are examined in monochromatic ultraviolet light using quartz reflection optics. The microscope image is photographically recorded and the negative is subsequently subjected to densitometric analysis. Each stage of the analytical procedure has been critically assessed to determine its validity and limitations. The method is ideally suited to the study of the removal of lignin from the wood cell wall during cooking and possesses other important applications in wood technology.     

THE USE OF LIVEWEIGHT-GAIN TRIALS FOR PASTURE EVALUATION IN THE TROPICS

An experiment is described in which liveweight gains were measured from two grass/legume pastures using small East African Zebu animals and also progeny of small East African Zebu cows crossed with Bos taurus bulls. Significantly higher production was obtained from cross-bred stock, which averaged 706 lb liveweight gain at per annum (791 kg/ha per annum), compared with 509 lb liveweight gain/ac per annum (571 kg/ha per annum) from Zebu stock, indicating that pasture management had advanced beyond the genetic potential of the local Zebu stock. The problems of designing, conducting and interpreting the results of such grazing trials are discussed.     

THE USE OF SHEEP FITTED WITH OBSOPHAGEAL FISTULAE IN GRAZING STUDIES

Studies were carried out on the effects of diurnal variation, fasting before sampling, and acclimatization to a sward on the chemical composition and in vitro OM digestibility of samples of extrusa collected from sheep fitted with oesophageal fistulae. The N content of extrusa samples increased markedly during the morning, and tended to decline again in the evening. The diurnal changes in digestibility were similar, but the variation was much smaller. There was no significant correlation between either N content or OM digestibility and grazing speed measured in bites per minute. There were no significant changes in either the N content or OM digestibility of successive samples of extrusa collected after over-night fasting, or after the sheep were introduced to a sward to which they were not accustomed, though the concentration of ash in the extrusa was significantly lower when the sheep were hungry than when they were not. The OM digestibility of the diet selected was approximately two units lower on the first two days after introduction to a new sward than the mean value of samples collected over the next 12 days.     

THE POTENTIAL OF LOTUS spp. FOR HILL LAND IN WALES

Thirty-four introductions of Lotus corniculatus and L. uliginosus , obtained from Europe, North America and New Zealand, were grown in plots with Aberystwyth S23 ryegrass at three centres in Wales (230–400 m a. s. 1.) to assess their potential as legumes for poor acid hill soils. The control white clover varieties were generally successful in these difficult conditions, whereas all the Lotus introductions were poor in two experiments and in the third experiment gave way to volunteer white clover by the third harvest year. These results indicate that none of the numerous Lotus varieties tested would be a useful addition to pasture seeds mixtures for hill lands in Wales.     

THE DISTRIBUTION OF LEAF AREA AND LIGHT INTENSITY WITHIN WHEAT CROPS DIFFERING IN ROW DIRECTION, ROW SPACING AND RATE OF SOWING; A CONTRIBUTION TO THE STUDY OF UNDERSOWING PASTURE WITH CEREALS

The distribution of leaf area and light intensity within wheat crops differing in row direction, row spacing and rate of sowing is described. Regardless of the manner of planting, 75% of the total leaf area was found within a band 31/2 in. wide, centred on the centre of the row. Light penetration within crops spaced at 14 in. was greater than within those spaced at 7 in. Light penetration within rows running N.–S. was more uniform and higher, particularly around noon, than within rows running E.-W. Within N.-S. rows the position midway between rows received the highest amount of light, but light decreased on either side until it was least beneath the rows. Within E.-W. rows at 14 in. spacing, maximum light was recorded at 101/2 in. on the southern side of a row, decreasing progressively as the row was approached.     

The effect of CCC on the initiation of potato tubers

Summary CCC, when applied to the roots of potato plants sufficiently early, brought forward the time of tuber initiation and growth. An increase in net assimilation rate at the time of rapid tuber growth was found for both CCC treated and control plants.     

Intersprout competition in the potato II. Competition for nutrients during pre-emergence growth after planting

Summary The influence of temperature, number of sprouts per tuber and concentration of supplementary inorganic nutrients on sprout growth and intersprout competition for tuber substrates during emergence was investigated in the potato varietyArran Pilot. The rate of sprout growth was found to be a positive function of initial tuber fresh weight. Dry weight data at emergence indicated that individual sprouts on multi-sprout tubers exhibited mutual interference for substrates from the tuber. The intensity of this interference increased with temperature in the range 7 25 C, but interference was reduced, and in some cases eliminated, by supplying inorganic nutrients in the rooting medium. It was concluded that a component of the inorganic nutrient solution used could replace the tuber factor the supply of which limited sprout growth. The effects of temperature and inorganic nutrient concentration on the partitioning of dry matter within the plant are deseribed and diseussed.

---

Zusammenfassung Die Einflüsse von Temperatur, ursprünglichem Knollengewicht, der Anzahl Keime pro Knolle und der Konzentration zus?tzlicher anorganischer N?hrstoffe auf das Keimwachstum sowie die Konkurrenz zwischen den Keimen um das Knollensubstrat w?hrend des Auflaufens wurden an der KartoffelsorteArran Pilot untersucht. Ween Knollen in ein n?hrstofffreies Medium gepflanzt wurden (Versuch 1), glich die Auflaufrate der Keime einer positiv linearen Funktion der Temperatur über einen Bereich von 7 C bis 25 C, aber sie war nicht signifikant beeinflusst durch die Zahl der Keime pro Knolle (Tabelle 1 und 2). Die einzelnen Keime von Knollen mit vielen Keimen konkurrierten um das Substrat der Mutterknolle, und die St?rke dieser Interferenz nahm mit steigender Temperatur w?hrend des Auflaufens zu (Abb. 1 und 2). In den Versuchen 2 und 3 wurde die Auflaufzeit durch die Beigabe einer anorganischen Volln?hrl?sung zum Wurzeln?hrboden verkürzt, aber sie wurde nicht signifikant beeinflusst durch die Zahl der Keime pro Knolle (Tabelle 4). Eine Erh?hung der N?hrstoffkonzentration in der anorganischen L?sung hatte eine Erh?hung des durchschnittlichen Trockengewichtes der Keime und des durchschnittlichen Trockengewichtes der Keime pro Knolle beim Auflaufen zur Folge (Abb. 4 und 5). In Versuch 2 setzte die Gabe von anorganischen N?hrstoffen die Intensit?t der Konkurrenz zwischen den Keimen um das Substrat aus der Knolle betr?chtlich herab, was darauf hindeutet, dass ein Bestandteil der verwendeten N?hrl?sung den “Knollenfaktor”, der das Keimwachstum begrenzt, ersetzen k?nnte. Im Versuch 3 dagegen wurde die Interferenz um die Substrate aus der Knolle durch die Konzentration der verwendeten N?hrstoffe nicht beeinflusst. Diese Abweichung zwischen den zwei Versuchen dürfte mit dem unterschiedlichen Grad der Interferenz zwischen den Wurzelsystemen der einzelnen Keime für die Zufuhr der verwendeten N?hrstoffe in Zusammenhang stehen (Tabelle 5). Die Temperatur nach dem Auspflanzen übte einen betr?chtlichen Einfluss auf die Verteilung der Trockensubstanz innerhalb des Stengel-systems aus. Anzahl, L?nge und Trockengewicht der Stolonen pro Keim (Abb. 3) und das Verh?ltnis des Stolonentrockengewichtes zum gesamten Trockengewicht der Stengel (Tabelle 3) waren in umgekehrter Bezichung von der Temperatur abh?ngig, in den Versuchen 1 und 3 stand das Verh?ltnis des Stolonentrockengewichtes zum gesamten Stengelgewicht in umgekehrter Bezichung zur Anzahl Keime pro Knolle: in Versuch 3 war diese Beziehung aber nicht signifikant beeinflusst durch die Konzentration der mineralischen N?hrstoffe. Diese Ergebnisse wiesen darauf hin, dass neben der Temperatur ein spezifischer Faktor in der Mutterknolle auf das Stolonenwachstum einwirkte und dass dieser Faktor nicht durch eine den Knollen zur Verfügung stehende mineralischen N?hrstoffl?sung ersetzt werden kann. In den Versuchen 2 und 3 erweiterte sich das Stengel/Wurzel-Verh?ltnis mit der Erh?hung der Konzentration der verwendeten anorganisehen N?hrstoffe, aber es war nicht signifikant beeinflusst durch die Zahl der Keime pro Knolle.

---

Résumé L’auteur a recherché, sur la variété de Pomme de terreArran Pilot, l’influence de la température, du poids initial du tubercule, du nombre de germes par tubercule et de la concentration en éléments nutritifs inorganiques supplémentaires sur la croissance du germe et la compétition entre les germes pour les réserves du tubercule pendant la levée. Quand les tubercules sont plantés dans un milieu dépourvu d’éléments nutritifs (expérience 1), la vitesse d’émergence d’un germe est une fonction linéaire positive de la température dans les limites de 7 C à 25 C mais n’est pas significativement influencéc par le nombre de germes par tubercule (tableau 1 et 2). Les germes des tubercules à plusieurs germes rivalisent individuellement pour les réserves du tubercule-mère et il se révèle que l’intensité de cette interférence s’accro?t avec l’augmentation de la température pendant l’émergence (Fig. 1 et 2). Dans les expériences 2 et 3, le temps d’émergence est réduit par l’application au milieu d’enracinement d’une solution nutritive complète inorganique, mais n’est pas significativement influencé par le nombre de germes par tubercule (Tableau 4). Une augmentation de la concentration en éléments nutritifs inorganiques entra?ne, lors de l’émergence, une augmentation du poids sec moyen d’un germe et une augmentation du poids sec moyen de tous les germes du tubercule (Fig. 4 et 5). Dans l’expérience 2 l’application d’éléments nutritifs inorganiques réduit l’intensité de la compétition entre germes pour les réserves du tubercule, ce qui signifie qu’un composant de la solution nutritive utilisée remplace le facteur du tubercule qui limite la croissance du germe. Cependant, dans l’experience 3, la compétition pour les réserves du tubercule n’est pas influencée par la concentration des éléments nutritifs appliqués. La différence dans les deux expériences peut provenir des différences dans le degré d’interaction entre les systèmes radiculaires des germes individuels pour les approvisionnements en éléments nutritifs appliqués (Tableau 5). La température après plantation exerce une influence considérable sur la répartition de la matière sèche dans le système radiculaire. Le nombre, la longucur et le poids sec de stolons par germe (Fig. 3) et le rapport du poids sec des stolons au poids total sec des germes (Tableau 3) sont des fonctions inverses de la température. Dans les expériences 1 et 3 le rapport poids sec des stolons au poids total des germes est inversément relié au nombre de germes par tubercule, mais dans l’expérience 3 ce rapport n’est pas significativement influencé par la concentration externe des éléments minéraux. Ces résultats indiquent qu’en plus des effets de la température, un facteur spécifique provenant du tubercule-mère influe sur la croissance du stolon, et que ce facteur ne peut être remplacé par un apport externe d’éléments nutritifs minéraux. Dans les expériences 2 et 3 le rapport bourgeon/ racine augmente avec l’accroissement de la concentration en éléments nutritifs inorganiques appliqués mais n’est pas significativement influencé par le nombre de germes par tubercule.

     

ABSORPTION OF RADIO–ACTIVE PHOSPHORUS FROM TAP–AND NODAL ROOTS OF WHITE CLOVER (TRIFOLIUM REPENS L.)

Absorption by the tap-root and by different nodal roots of white clover S100 and translocation of ³²P were investigated to find to what extent nodal roots can compensate for the absence of a tap-root. ³²P absorbed from the tap-root was distributed evenly within the whole plant. When translocation from the 2nd and 7th nodal roots was studied, similar distribution was obtained only from the nodal root closer to the centre of the plant. The backward movement of ³²P absorbed from nodal roots at the base of the plant increased as the root size increased. Removal of tap-roots resulted in temporary depression of translocation; its effect disappeared within 3 weeks.
It is concluded that the ability of nodal roots to compensate for loss of the tap-root depends on their position and size.     

THE UTILIZATION OF GRASS FED INDOORS TO YOUNG BEEF CATTLE, WITH OR WITHOUT SUPPLEMENTARY BARLEY

An experiment was carried out in which grass was cut and fed indoors, with or without supplementary barley. Seven animals were allocated to each of the following treatments: (1) grass ad lib . without supplementary barley; (2) grass ad lib . with supplementary barley at the rate of 0.5 lb/100 lb bodyweight; (3) grass ad lib . with supplementary barley at the rate of 1 lb/100 lb bodyweight. Supplementary barley increased total dry-matter intake and improved daily liveweight gain and food conversion rate. The mean daily liveweight gains for the 17-week experimental period were 1.60, 1.91 and 2.20 lb and the corresponding feed dry-matter conversion ratios were 6.18, 5.67 and 5.55 for Treatments 1, 2 and 3, respectively.
The dry-matter digestibility of the diets was slightly higher when the grass was supplemented with barley than when grass alone was given. Although barley depressed the digestibility of protein, the absolute and the proportionate retention of nitrogen was increased. This was particularly marked on Treatment 3, presumably because of the higher net energy value of the diet containing the greater amount of barley. The N-retention data corresponded to the rates of liveweight gain obtained.