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81.
T M Harty S A Ferenc D B Copeman C H Courtney 《American journal of veterinary research》1989,50(1):169-172
Dermal microfilariae recovered from specimens obtained from umbilical and cervical sites of cattle infected with adult Onchocerca gutturosa alone or with adults of O gutturosa and O lienalis were measured and compared with uterine microfilariae obtained directly from gravid female worms of each species. Uterine microfilariae of O gutturosa were longer than dermal microfilariae obtained from cattle harboring only adults of O gutturosa. Dermal microfilariae were recovered from umbilical and cervical sites in these cattle. Those found at the cervical site had lengths equal to or greater than lengths of microfilariae recovered from the umbilical site. There was a significant (P less than 0.0001) shift in length across populations of microfilariae of O gutturosa from various sites in its bovine host, with a progressive decrease in length between microfilariae recovered from the worm's uterus, microfilariae from the cervical dermis, and microfilariae from the umbilical dermis, respectively. A similar direct comparison was not possible for microfilariae of O lienalis, because none of the cattle was infected with only adult worms of this species. In an indirect comparison, microfilariae of O lienalis were identified at the umbilicus, but their presence in the cervical region could not be determined unequivocally because of confounding of microfilariae length by concurrent infection with O gutturosa. Uterine microfilariae from O lienalis were longer than uterine microfilariae of O gutturosa, although a degree of overlap in the range of measurements existed between species. 相似文献
82.
83.
将72只日龄为14d的“星布罗”肉用仔鸡随机分为实验和对照两组,每组36只。实验组接受强度为151x的1L:3D间歇性白炽灯光照,对照组为自然光照。两组的饲喂条件相同。结果:与对照组相比,实验组体重略高而料肉比略低,实验组鸡胰腺A、B细胞的内分泌颗粒减少,A颗粒粒芯周围出现大致均匀的晕轮,B颗粒粒芯呈现不典型的针状、棒状或环状,PP细胞的内分泌颗粒明显增大、增多,胞浆中的粗面内质网、高尔基复合体和线粒体等数量增多。两组鸡的D细胞无明显差异。34日龄的实验组鸡血浆胰岛素含量明显高于对照组,且与体重呈正相关。 相似文献
84.
T N Tully S M Shane R P Poston J J England C C Vice D Y Cho B Panigrahy 《Avian diseases》1992,36(3):808-812
Eastern equine encephalitis (EEE) was diagnosed in a flock of emus in southeastern Louisiana. The outbreak involved juvenile and adult breeders ranging in age from 20 to 36 months, with an attack rate of 76% and a case fatality rate of 87%. The diagnosis was confirmed by isolation and characterization of the viral agent, and by detection of EEE antibody in two recovered emus. High mortality was preceded by marked depression, hemorrhagic diarrhea, and emesis of blood-stained ingesta. On postmortem examination, hemorrhagic enteritis and multiple petechia of viscera were observed. Microscopic changes included severe necrosis of hepatocytes, intestinal mucosa, and necrotizing vasculitis of the spleen and lamina propria of the intestine. No nervous system lesions were observed. This outbreak occurred concurrently with EEE in horses and was attributed to unseasonably heavy rainfall with an abundance of arthropod vectors and proximity to free-living reservoir host species. 相似文献
85.
B S Bhogal G A Miller A C Anderson E J Jessee S Strausberg R McCandliss J Nagle R L Strausberg 《Veterinary immunology and immunopathology》1992,31(3-4):323-335
A genetically engineered Eimeria tenella antigen (GX3262), produced as a fusion protein with beta-galactosidase and identified with a monoclonal antibody, induced partial but significant protection in young broiler chickens against experimental E. tenella and Eimeria acervulina infections. The antigen appears to share a T-helper cell epitope with the parasite as evidenced by (a) booster inoculation with either the recombinant antigen or with a small number of live oocysts enhanced the protective immunity in GX3262 primed chickens, and (b) ability of the antigen to induce in vitro stimulation of T-cells from chickens immunized with antigen or parasite. These observations suggest the feasibility of a single vaccination of 1 or 2-day-old broilers with GX3262 to induce an acceptable degree of protective immunity. The implications of the observations reported here are far reaching in terms of a practical coccidiosis vaccine for poultry, and show for the first time that 1-day-old broiler chickens can be efficiently vaccinated with a recombinant antigen against one or more species of Eimeria. 相似文献
86.
Plasma triglyceride concentration during intravenous infusions of propofol and Intralipid in sheep 总被引:1,自引:0,他引:1
B. M. Q. Weaver D. Raptopoulos † G. E. Staddon W. W. Mapleson‡ 《Veterinary anaesthesia and analgesia》1996,23(1):15-19
Plasma triglyceride concentrations were measured in sheep given Intralipid or propofol, which is carried in a vehicle very similar to 10% Intralipid. A bolus dose was administered followed immediately by an infusion of the same agent for 2 h. In the animals that received propofol, the measured concentration increased by a mean amount of 3.39 mmol/l when the infusion rate was l ml/min (Group Pl) and by 7.13 mmol/l when it was 2 ml/min (Group P2). When 10% Intralipid was administered and infused at 1 ml/min (Group I10), the measured concentration increased only by 0.95 mmol/l. One hour after stopping the infusion, the excess of measured concentration over baseline had decreased in the Pl and I 10 groups to 0.52 and 0.13, respectively, of the corresponding maximum excess. The method adopted for measuring plasma triglycerides is widely used in hospitals; however, an incidental observation revealed that it is inappropriate in the presence of injections of propofol or Intralipid. Despite this, evidence and argument are presented to support the conclusion that, with propofol, plasma triglyceride concentrations increased more rapidly during the infusions and returned to baseline more slowly than with a corresponding amount of Intralipid. 相似文献
87.
采用免疫组化BA法,对流行性出血热病毒(EHFV)气溶胶感染乳鼠脏器组织中的病毒动态分布进行了观察,并对其抗体消长进行了测定。结果表明:①BA法为病理诊断EHFV感染提供了一种准确的手段;②EHFV可通过气溶胶途径感染乳小鼠,乳小鼠感染EHFV的LD50值可作为环境浓度的参考值;③EHFV气溶胶途径感染乳鼠后,4h即可在心、肺,1d在嗅球部检出,并可在其组织细胞中增殖,经血循、嗅神经、单核巨噬细胞系统等多途径扩散,造成多组织脏器的泛发性感染。感染后12~14d,病毒检出率和病毒量均达峰值。动物自身免疫系统对EHFV有一定的清除能力;④感染乳鼠脑、肺、肾等脏器组织的病变与人类感染EHFV的病变有一定的相似性,提示可作为人EHFV的模型动物;⑤抗原检出率高于抗体检出率,ELISA法检出率高于IFA法检出率。 相似文献
88.
Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line. 下载免费PDF全文
K F Lawson H Chiu M Matson P Bachmann J B Campbell 《Canadian journal of veterinary research》1992,56(2):135-141
In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals. 相似文献
89.
Dingell v. Baltimore 总被引:1,自引:0,他引:1
B J Culliton 《Science (New York, N.Y.)》1989,244(4903):412-414
90.
J D Fikes R A Lovell M Metzler W B Buck 《Journal of the American Veterinary Medical Association》1989,194(4):543-544
Two male English Setters were noticed to be breathing rapidly, hyperexcitable, and atactic after roaming a rural area for 2 hours. Both dogs' cost were stained with yellow liquid. One dog died while en route to the veterinarian. Treatment was begun for the surviving dog for what was initially diagnosed to be organophosphorus or carbamate insecticide toxicosis. Before the diagnosis could be confirmed, the second dog died. The yellow liquid on the dogs' skin was identified as dinoseb in high concentrations. Dinoseb is an acutely toxic, substituted dinitrophenolic herbicide believed to act as an uncoupler of electron transport from oxidative phosphorylation. 相似文献