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21.
The clinical course of a feline leukaemia virus (FeLV)-negative and feline immunodeficiency virus (FIV)-positive cat affected with a large granular lymphocyte lymphoma is presented. Cyto-logical examination showed neoplastic cells in the pleural effusion and in two abdominal masses. Bone marrow and peripheral blood were moderately involved and chemotherapy was used to control the tumour. Cytochemistry, immunohis-tochemistry and ultrastructural studies were applied to define the cellular lineage; cytochemistry suggested a T-cell lineage.  相似文献   
22.
We have developed an antibody detection enzyme-linked immunosorbent assay (ELISA) for the identification of animals infected by feline immunodeficiency virus (FIV). The ELISA solid-phase antigen consists of recombinant FIV gag proteins expressed in bacteria. The proteins are purified from bacterial lysates as insoluble inclusion bodies. In the case of bacterially expressed p24gag, it is shown that all of the linear, sequential epitopes presented by viral p24 during infection are retained. Purified preparations can be substituted for solid-phase whole virus in the IDEXX PetChektm immunoassay. The antibody ELISA duplicates the sensitivity and specificity of the whole virus based PetChek plate assay.  相似文献   
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The oscillatory potentials (OP) on the b-wave of the canine ERG are characterized. Normal values for an OP-index, implicit times and periodicity are given. The OP-index increases during dark adaptation as well as with increasing stimulus intensities for white light flashes. Scotopic blue and red stimuli, although balanced for the maximum b-wave amplitude, surprisingly result in higher OP-indices for red flashes. Implicit times decrease for all OP with increasing stimulus intensities. Scotopic balanced red light results in OP with markedly reduced implicit times compared to blue light stimulation. The intervals between two OPs remain constant during dark adaptation, however, intervals between OPs with longer implicit times tend to be shorter. In contrast, the b-waves for scotopic balanced stimuli show rhythmic oscillations with constant intervals between them, the intervals being shorter for blue light than for red light. The intervals between OPs tend to become shorter with increasing stimulus intensities. This investigation indicates that OPs are influenced but not generated by photoreceptors. The examination of a dog with optic nerve hypoplasia indicates that the ganglion cells do not contribute to the biogenesis of OPs. The prognostic value of OPs in the early diagnosis for hereditary retinal degenerations in the dog is discussed.  相似文献   
25.
A sensitive and precise immunoassay for equine serum amyloid A protein (SAA) was established and used to determine, for the first time, the circulating concentration of this protein in health and disease. As in other species, equine SAA was present only at trace levels in healthy animals but behaved as an extremely sensitive and rapidly responding acute phase reactant following most forms of tissue injury, infection and inflammation, objectively reflecting the extent and activity of disease. Measurements of SAA should make a significant contribution to diagnosis and management of viral and bacterial infection in horses, and routine serial assays could provide an objective criterion for monitoring prospectively the general health of horses in training and racing.  相似文献   
26.
Comprehensive studies into domestic pigs and wild boars together with literature data provided a basis for an assessment of aerobic metabolic capacity (VO2max) of swine. The values quoted, from 20 to 94 ml/min-1/kg, had been due to several factors of methodological approach, growth, training, and nutrition as well as to pathophysiological aspects. For full capacity utilisation of VO2max, untrained pigs can be challenged at belt velocities between 0.8 and 1.8 m/s-1 and trained animals at 5 m/s-1.  相似文献   
27.
A urinary bladder mass in a 12-year-old spayed female West Highland White Terrier was diagnosed after exploratory surgery and biopsy as a transitional cell carcinoma. Four months later the dog presented with an ulcerated plaque-like cutaneous lesion at the previous surgical incision site; concurrent inguinal lymphadenopathy and recurrence of the urinary bladder mass were identified. Transitional cell carcinoma was diagnosed at all 3 sites. Although a definitive relationship cannot be established between the initial surgery for urinary bladder mass and the resultant subcutaneous lesion, surgical implantation should be considered as a source for the neoplastic cells.  相似文献   
28.
樟子松枯梢病的防治研究   总被引:6,自引:0,他引:6  
樟子松移植苗枯梢病Sphaeropsis sapinea可以通过药剂有效地进行防治。移植前以50%多菌灵wp500倍液浸苗;在人工林中,内吸性杀菌剂-甲基托布津、多菌灵为首选药剂;在树木新梢生长停止期和展叶中期(辽西北为6月初和6月中旬),70%甲基托布津wp 1000倍液或50%多菌灵wp 500倍液防治2次。保护性杀菌剂-百菌清效果不佳。适时的卫生伐对该病有明显的抑制作用。  相似文献   
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30.
腐蚀管道剩余寿命的性能衰减模型研究   总被引:4,自引:0,他引:4  
管道在长期的运行过程中,由于介质和土壤的腐蚀性,管材的微塑性变形、结构转变和渗氢等。不仅会使管道出现各种腐蚀损伤,而且还存在着老化效应和氢化作用,导致管材的脆变并改变材料的塑性,使机械性质参数发生衰退,从管材性能衰减和腐蚀损伤角度研究了管道的剩余寿命,提出了根据结构承载能力的极限值确定塑性变形时工作寿命的方法,为了确定剩余使用寿命,对剪切损伤率和屈服性能比等概念进行定义,在掌握管材金属剪切损害率和屈服性能比的时间特性基础上,建立了剩余寿命计算模型。并给出了算例。  相似文献   
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