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971.
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Diversity of the coat protein-coding region among Ilarvirus isolates infecting hop in Australia 总被引:1,自引:0,他引:1
D. R. Crowle S. J. Pethybridge G. W. Leggett L. J. Sherriff C. R. Wilson † 《Plant pathology》2003,52(5):655-662
Coat protein (CP) sequences of 17 Ilarvirus isolates were obtained from hops at three farms in Tasmania, Australia. Phylogenetic analysis of these sequences and additional database sequences indicated several Apple mosaic virus (ApMV) isolate clusters distinct from Prunus necrotic ringspot virus (PNRSV): one containing isolates from apple; one containing a single isolate from almond; a third containing Australian hop isolates of the 'apple' serotype and a German isolate of unknown origin; and a fourth containing Australian hop isolates of the 'intermediate' serotype. Isolates from hop, pear and prune from the Czech Republic either formed a fifth grouping, or were divergent members of the 'intermediate' serotype group. Deduced amino acid (aa) residue differences between the coat proteins of the two hop isolate serotype groups were highlighted as possible regions of serological differentiation. No evidence for coinfection of plants with both serotypes was found. Tests of ApMV-infected hop buds using the Shirofugen flowering cherry assay revealed a possible differentiation of the two strains based on hypersensitivity. Because of serological similarities to PNRSV, these viruses have commonly been reported as strains of PNRSV. However, this study shows ilarviruses from Australian hops are strains of ApMV, but distinct from those infecting Malus spp. 相似文献
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Prins B Loewen KG 《The Canadian veterinary journal. La revue veterinaire canadienne》1991,32(8):487-491
The results of laboratory examination of 20460 specimens for the diagnosis of rabies by the standard fluorescent antibody and mouse inoculation tests over a five year period are presented. Specimens were received from British Columbia, Alberta, Saskatchewan, Manitoba, and the Yukon and Northwest Territories. Of those examined, 9.96% were positive. The main reservoirs of rabies were skunks, bats, and foxes. During this observation period a rabies epizootic occurred in skunks in Saskatchewan. When both tests were performed, the fluorescent antibody test was found to agree with the mouse inoculation test in over 99% of cases. 相似文献
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Spake Rebecca Soga Masashi Kawamura Kazuhiro Cooke Robert S. Yamaura Yuichi Eigenbrod Felix 《Landscape Ecology》2020,35(5):1055-1071
Landscape Ecology - Functional responses to landscape heterogeneity are context-dependent, hampering the transferability of landscape-scale conservation initiatives. Japan provides a unique... 相似文献