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71.
Analyses of the genetic diversity of matsutake isolates collected from different ecological environments in Asia 总被引:1,自引:0,他引:1
Dapeng Bao Ayumi Koike Fangjie Yao Katsuji Yamanaka Tadanori Aimi Yutaka Kitamoto 《Journal of Wood Science》2007,53(4):344-350
Matsutake mushrooms are able to grow in two kinds of ecological environments: coniferous forests and broadleaf forests. Japanese
matsutake is a form of the matsutake mushroom that mainly appears in coniferous forests in the mid-latitude regions of Asia.
In contrast, matsutake from Yunnan and Sichuan Provinces of China grows in broadleaf forests in low-latitude regions. The
taxonomic relationship and the genetic distance between these two types of matsutake remain unclear. Here, we compared the
random amplification of polymorphic DNA (RAPD) data and the sequences of the internal transcribed spacer (ITS) region and
of variable domains V4, V6, and V9 of mitochondrial small subunit ribosomal DNA (mt SSU rDNA) to determine the genetic differences
of Tricholoma matsutake strains collected from different ecological and geographic environments in Asia. We found few differences in the sequences
of the variable domains, implying that all matsutake strains are the same biological species regardless of their host specificity
and geographical differences. This conclusion is also supported by the sequences of the ITS region. On the other hand, RAPD
analysis revealed that the matsutake mushrooms collected from different ecological environments have genetic differences and
represent distinct varieties. 相似文献
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73.
Asano K Takagi K Haneishi A Yamamoto T Tanaka T Noguchi T Nakamura S Yamada K 《Journal of agricultural and food chemistry》2011,59(24):13360-13364
74.
Naoki Isobe Ayumi Shibata Hirokazu Kubota Yukinori Yoshimura 《Animal Science Journal》2013,84(11):751-756
The present study was undertaken to examine whether potential levels of innate immune factors (lingual antimicrobial peptide (LAP), lactoferrin (LF) and lactoperoxidase (LPO)) in colostrum are associated with subsequent milk somatic cell count (SCC) in dairy cows. Quarter milk samples were collected daily for 1 week postpartum to measure LAP and LF concentrations and LPO activity. SCC in milk was determined weekly for 2 months postpartum and its correlations to concentrations of LAP and LF and LPO activity were examined. Only small variations of all immune factors were found among four udders in each individual cow, whereas there were great differences in these factors among cows. Negative correlation was detected only between LPO activity and mean and maximum SCC, whereas its relationship was not significant. LAP and LF concentrations were significantly correlated positively to mean, maximum and minimum SCC. These results suggest that the great difference in innate immune factors among animals and high LAP and LF concentrations in colostrum may be associated with subsequent high incidence of SCC increase. 相似文献
75.
Physical, chemical, and biological properties of s-allylcysteine, an amino acid derived from garlic. 总被引:5,自引:0,他引:5
Yukihiro Kodera Ayumi Suzuki Osamu Imada Shigeo Kasuga Isao Sumioka Atsushi Kanezawa Nobuo Taru Masanori Fujikawa Shinji Nagae Koji Masamoto Katsuhiko Maeshige Kazuhisa Ono 《Journal of agricultural and food chemistry》2002,50(3):622-632
Physical, chemical, and biological properties of S-allylcysteine (SAC) were investigated. SAC showed stable properties under tested conditions, and its acute/subacute toxicity was very minor in mice and rats (LD(50) value >54.7 mM/kg po; >20 mM/kg ip). The pharmacokinetics of SAC was investigated after oral administration of garlic supplement containing SAC to human volunteers. SAC from garlic consumption was rapidly absorbed from the gastrointestinal tract, however, the half-life and excretion time were more than 10 h and 30 h, respectively. 相似文献
76.
Sangho Kim Kenji Hosoya Ayumi Kobayashi Masahiro Okumura 《Veterinary and comparative oncology》2019,17(1):61-68
Peripheral blood stem cell (PBSC) transplantation following consolidation therapy is a feasible treatment option for canine haematological malignancies. In veterinary medicine, haematopoietic stem cells are generally mobilized into peripheral circulation using a granulocyte colony‐stimulating factor (G‐CSF). This pilot study aimed to evaluate the haematopoietic stem cell mobilization effect of three different regimens for PBSC apheresis with Spectra Optia continuous mononuclear cell (CMNC) protocol in healthy dogs. Stem cell mobilization was performed using high‐dose plerixafor (CXCR‐4 inhibitor) alone, a G‐CSF alone, or a combination of the low‐dose plerixafor and G‐CSF. Three dogs were assigned to each mobilization protocol. Regardless of the mobilization protocol, the total blood volume processed was uniformly set as 270 mL/kg and many PBSCs, defined as CD34+/CD45dim cells, within the apheresis product were compared. Changes in complete blood count, PBSC counts, and blood chemistry analysis were monitored before, during, and after apheresis. All dogs tolerated the apheresis procedure using the Spectra Optia system with minimal adverse effects. The mean PBSC counts of the apheresis products for plerixafor, G‐CSF, and the combination groups were 1.3 ± 0.24, 4.2 ± 0.47, and 6.4 ± 0.9 × 106 cells/kg, respectively. The apheresis procedure using Spectra Optia CMNC protocol in dogs is safe and feasible. Furthermore, PBSC mobilization with a combination of G‐CSF and plerixafor appeared more effective than either compound alone in mobilizing PBSC to the peripheral blood in dogs. 相似文献
77.
Tetsuya KOMATSU Erina YOSHIDA Ayumi SHIGENAGA Nozomi YASUIE Shintaro UCHIYAMA Yuji TAKAMURA Kennosuke SUGIE Kumiko KIMURA Makoto HARITANI Tomoyuki SHIBAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1113
One calf died (No. 1) and another was euthanized following astasia (No. 2). Histopathological examination revealed suppurative meningoencephalitis in these calves. Klebsiella pneumoniae antigens were detected in lesions. Thymocytes were decreased in the thymus cortex in both cases. 16S rRNA gene sequencing of the No. 1 isolate and bacterial extracts from formalin fixed paraffin embedded sections of No. 2 revealed that both samples were K. pneumoniae. The No. 1 isolate showed multidrug resistance against penicillin antibiotics, fosfomycin, streptomycin, macrolide antibiotics, tetracycline antibiotics, and clindamycin. Immunosuppression is a significant septicemic K. pneumoniae infection risk factor. Our study provides new aspects regarding K. pneumoniae infections in cattle, bacterial meningoencephalitis differentiation, and K. pneumoniae and bacterial meningoencephalitis treatments. 相似文献
78.
Yoshimichi Fukuta Kunihiko Konisho Sachiko Senoo-Namai Seiji Yanagihara Hiroshi Tsunematsu Ayumi Fukuo Takashi Kumashiro 《Breeding Science》2012,62(1):27-37
A total of 18 rainfed upland New Rice for Africa (NERICA) varieties were categorized as the heavy panicle and low tillering types and early heading, in compared with 32 different varieties. These chromosome components were clarified using 243 SSR markers which showed polymorphism among NERICA varieties and their parents, CG 14 (O. glaberrima Steud.) and one of the recurrent parents, WAB-56-104 (O. sativa L.). NERICA varieties were classified into three groups, which corresponded with these parents’ continuation including two exceptions, NERICAs 14 and 17, by a cluster analysis using polymorphism data of SSR markers and 14 differential markers among them were selected to classify NERICA varieties. However, three groups: NERICAs, 3 and 4, NERICAs, 8, 9 and 11 and NERICAs, 15 and 16 were not distinguishable. Association analysis was carried out for characterization of NERICA varieties by using SSR markers genotype and phenotype of agronomic traits. A total of 131 quantitative trait loci between SSR markers and 11 agronomic traits were detected. The characteristics of early maturity and heavy panicle of upland NERICA varieties were succeeded from Asian rice varieties and the characteristics of high dry matter production and late heading were introduced from CG 14 and the other varieties. 相似文献
79.
Sybuia Priscila Ayumi de Castro-Prado Gabriela de Carvalho Nunes William Mário Zanutto Carlos Alexandre Kaneshima Edilson Nobuyoshi Soares Dartanhã José da Silva Franco Claudinéia Conationi de Freitas Mathias Paulo Cezar de Castro-Prado Marialba Avezum Alves 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):937-950
European Journal of Plant Pathology - Charcoal rot is an economically important fungal disease of economically important crops. Several species of the Macrophomina genus, such as M.... 相似文献
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