The penetration of soil by mycelial strands of ectomycorrhizal fungi

Soil factors affecting growth of mycelial strands from ectomycorrhizas into surrounding soil were studied experimentally. Treated soil cores were inserted into root boxes for 4–6 weeks, then infiltrated with gelatin, sectioned, and mycelial strands harvested. Very large differences in mycelial strand growth (measured by weight) occurred between different soils, some soils completely inhibiting growth of strands of the mycorrhizal fungus Rhizopogon luteolus. Compaction of soil reduced mycelial strand penetration greatly e.g. by 80 per cent in one experiment. Soil sterilization ameliorated compaction effects. High soil phosphorus tended to increase strand growth but nitrogen level and Pseudomonas fluorescens. a common soil bacterium, had small or inconsistent effects.     

THE EFFECT OF AUTUMN MANAGEMENT ON WINTER DAMAGE AND SUBSEQUENT SPRING PRODUCTION OF SIX VARIETIES OF LOLIUM PERENNE GROWN AT CAMBRIDGE

Six perennial ryegrass varieties which were known to differ in winter hardiness were subjected to ten managements comprising two N rates and fire autumn cutting treatments. Autumn yields and soluble carbohydrate contents were measured, also the amount of winter damage in February and subsequent spring yields. The winter was mild, herbage damage was slight and no plant deaths were recorded, but significant differences were found between cutting treatments and varieties. The amount of winter damage increased with the length of the autumn rest period and with the degree of winter dormancy of individual varieties, indicating that natural senescence was a more important causative agent than frost action. Spring yields were affected by the amount of winter damage but there were considerable interactions between varieties and environments. The results are discussed in relation to autumn management practices, choice of variety for different locations and measurements of winter hardiness. The induction of winter dormancy by day length or low temperature reactions is considered in the breeding of varieties for maximum production in different parts of the country.     

THE EFFECT OF PESTICIDES ON THE YIELD AND BOTANICAL COMPOSITION OF A NEWLY-SOWN RYEGRASS LEY AND OF AN OLD MIXED PASTURE

Newly-sown S24 perennial ryegrass at Hurley and old established ryegrass-dominant mixed pasture at Rothamsted were treated with several pesticides, singly and in combination, over the period 1969–73. Yields were either unaffected or increased by up to 30% in any one year, depending on the treatment, the newly-sown sward giving the more consistent response from year to year.     

THE EFFECT OF NITROGEN, STOCKING RATE AND GRAZING METHOD ON THE OUTPUT OF PASTURE GRAZED BY BEEF CATTLE

An experiment was conducted in 1971 and 1972 to study the effects of two levels of fertilizer N (50 and 504 kg/ha) on the productivity of pastures grazed by young beef cattle. Two stocking rates were imposed at the lower N level and three at the higher. In addition to rotational grazing, set-stocking and an integrated grazing-conservation system were included. The responses/kg N were approximately 1 kg live-weight gain, 19–24 Meal ME and 8–9 kg DM. Maximum yields of 1200–1300 kg live-weight gain/ha and 25,000–27,000 Meal ME/ha were recorded. Productivity of set-stocked pasture was similar to rotational grazing at the high N level, but lower at low N. The integrated system yielded comparable results to high-N rotational grazing. Performances and herbage intakes per animal reflected the stocking rates imposed.     

THE INFLUENCE OF SUPPLEMENTARY FEEDING ON THE HERBAGE INTAKE AND GRAZING BEHAVIOUR OF DRY COWS

The herbage intake and grazing behaviour of 4 dry cows on autumn grass receiving 2, 4, 6 or 8 kg concentrates/day, was assessed in a 4×4 Latin Square experiment. Total OM intake increased from 11±5 kg/day with 2 kg concentrates to 13±6 kg/day with 8 kg concentrates. The average supplementary effect was 0±46 kg increase in OM intake/kg OM fed as concentrates. Minutes per day spent grazing decreased from 495 with 2 kg concentrates to 359 with 8 kg. The mean decline was 22 min./kg concentrates or 28 min./kg OM in concentrates eaten. Herbage was eaten at an average rate of 47 min./kg OM.     

THE EFFECT OF CUTTING HEIGHT AND NITROGEN LEVEL ON THE YIELD, IN VITRO DIGESTIBILITY AND CHEMICAL COMPOSITION OF ITALIAN RYEGRASS SWARDS

Three cutting heights, 2±5, 7±6 and 12±7 cm (1, 2±5 and 5 in.) and three levels of fertilizer N, 168, 280 and 392 kg N/ha (150, 250 and 350 Ib N/ac) were imposed on a sward of Italian ryegrass (Lolium multiflorum) cv. Irish. Lowering the cutting height and increasing the level of applied N increased the yield of herhage DM. Increasing the level of applied N had a greater effect on the chemical composition of the herbage than altering the cutting height     

Adsorption and activity of chitinase on kaolinite

Chitinase is adsorbed on kaolinite below the isoelectric point of the enzyme, pH 6.8. Maximum adsorption is reached below pH 4.6. The amount of chitinase adsorbed to kaolinite depends on the concentration and on the ratio of enzyme to kaolinite. Adsorption results in reduction of chitinase activity. the extent of which depends on the amount of kaolinite present, on pH, and on the length of exposure time. Upon sorption of chitinase on kaolinite the optimal pH for activity is increased from pH 4.7 to 5.7.     

Fertilizing fish ponds. I — Chemistry of the waters

Fertilization experiments were carried out in the Mex fish farm using superphosphate, ammonium nitrate and organic manure as fertilizers.The water was analysed monthly for inorganic phosphates, nitrates and silicates, in addition to chlorosity and pH.The ponds fertilized with chemical fertilizers exhibited a more rapid consumption of nutrients than the pond fertilized with organic manure.Phosphates and nitrates disappeared from the ponds after about two days of addition.