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51.
Effects of medetomidine and xylazine on intraocular pressure and pupil size in healthy Beagle dogs
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Teppei Kanda Ayano Iguchi Chieko Yoshioka Haruka Nomura Kanako Higashi Minami Kaya Rie Yamamoto Tomoko Kuramoto Toshinori Furukawa 《Veterinary anaesthesia and analgesia》2015,42(6):623-628
ObjectiveTo determine the effects of intramuscular (IM) administration of medetomidine and xylazine on intraocular pressure (IOP) and pupil size in normal dogs.Study designProspective, randomized, experimental, crossover trial.AnimalsFive healthy, purpose-bred Beagle dogs.MethodsEach dog was administered 11 IM injections of, respectively: physiological saline; medetomidine at doses of 5, 10, 20, 40 and 80 μg kg−1, and xylazine at doses of 0.5, 1.0, 2.0, 4.0 and 8.0 mg kg−1. Injections were administered at least 1 week apart. IOP and pupil size were measured at baseline (before treatment) and at 0.25, 0.50, 0.75, 1, 2, 3, 4, 5, 6, 7, 8 and 24 hours post-injection.ResultsA significant decrease in IOP was observed at 6 hours after 80 μg kg−1 medetomidine compared with values at 0.25 and 0.50 hours, although there were no significant changes in IOP from baseline. In dogs treated with 8.0 mg kg−1 xylazine, significant reductions in IOP were observed at 4 and 5 hours compared with that at 0.25 hours after administration. In dogs treated with 5, 10, 20 and 40 μg kg−1 medetomidine and 0.5, 1.0 and 2.0 mg kg−1 xylazine, there were no significant changes in IOP. Pupil size did not change significantly after any of the medetomidine or xylazine treatments compared with the baseline value.Conclusions and clinical relevanceLow or moderate doses of medetomidine or xylazine did not induce significant changes in IOP or pupil size. In contrast, high doses of medetomidine or xylazine induced significant changes up to 8 hours after treatment, but values remained within the normal canine physiological range. The results of this study suggest a lack of significant change in IOP and pupil size in healthy dogs administered low or moderate doses of xylazine or medetomidine. 相似文献
52.
Satoshi Taba Ayano Takara Kanami Nasu Nao Miyahira Tetsuya Takushi Zen-ichi Moromizato 《Journal of General Plant Pathology》2009,75(2):160-162
An outbreak of black mottle and dieback on basil (Ocimum basilicum L.; Lamiaceae) was recorded in a greenhouse in Okinawa Prefecture, Japan during 2004. The causal agent was identified as
Alternaria alternata (Fr.) Keissler based on morphological characters and growth temperature. This report is the first of Alternaria leaf spot
of basil caused by A. alternata. 相似文献
53.
Katsumi Togashi Yosuke Taga Kazunobu Iguchi Takuya Aikawa 《Journal of Forest Research》2008,13(2):127-131
Bursaphelenchus mucronatus Mamiya et Enda has been recovered for the first time from adults of the cerambycid beetle, Monochamus urussovi (Fischer), in Hokkaido, Japan. The nematode was also recovered from the inner bark of Picea jezoensis (Siebold et Zuccarini) Carrière and Abies sachalinensis (Fr Schmidt) Masters infested with M. urussovi larvae. PCR–RFLP analysis indicated that B. mucronatus in Hokkaido is the European type. 相似文献
54.
Ayano KUDO Sho YOSHIMOTO Hiromitsu YOSHIDA Yusuke IZUMI Satoshi TAKAGI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):784
Cancer-associated fibroblasts (CAFs) play an essential role in tumor invasion and metastasis. In dogs, the biological features of CAFs have not been well characterized. The purpose of this study was to investigate differences in the biological activities of canine CAFs and normal fibroblasts (NFs), and their influence on the migration and invasion of cancer cells. Canine CAFs and NFs were harvested from surgically-resected malignant epithelial tumor tissues and skin tissues of dogs. A wound-healing assay was conducted to compare the migratory and invasive abilities of canine CAFs and NFs. The results of this study showed that canine CAFs have a greater migratory and invasive ability than NFs. To observe the indirect and direct interactions between fibroblasts and cancer cells, Boyden chamber assay and 3D co-culture with collagen gel were conducted. The number of migrated and infiltrated cancer cells co-cultured with canine CAFs was greater than that with NFs. In the 3D co-culture, cancer cells showed noteworthy proliferation on the surface of gels containing canine CAFs and invasion into the gel. On the other hand, no infiltration of cancer cells into the gel containing NFs was observed. It was suggested that canine CAFs activate migration and invasion of cancer cells and promote the infiltration of cancer cells into collagen gels. 相似文献
55.
Ayano HIRAKO Jin SUN Satoshi FURUKAWA Takashi TAKEUCHI Akihiko SUGIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1565-1571
Two-day-old rats were treated with subcutaneous injections of methotrexate (MTX) 5 mg/kg
and 150 mg/kg, and their rostral migratory streams (RMS) were examined time-dependently.
MTX treatment increased pyknotic and TUNEL-positive cells and decreased mitotic and
phospho-Histone H3-positive cells at almost all time points in the vertical arm, elbow and
horizontal arm regions of the RMS. There were more TUNEL-positive cells ratio in the MTX
150 mg/kg group than in the MTX 5 mg/kg group. Treatment with MTX 150 mg/kg decreased the
cellularity in the vertical arm region on Postnatal day (PD) 4, but that with the MTX 5
mg/kg did not. TUNEL-positive cells ratio was the highest in the vertical arm region,
followed by elbow and horizontal regions in both MTX-treated groups. TUNEL-positive cells
ratio in the vertical arm and elbow regions reached their peaks on PD 4 in both
MTX-treated groups, and both MTX-treatments significantly decreased Phospho-Histone
H3-positive cells ratio on PDs 2.5 and 3 in the vertical arm, elbow and horizontal arm
regions. The phospho-Histone H3-positive cells ratio in the vertical arm region recovered
on PD4 in the MTX 150 mg/kg group. These findings suggested that RMS required a great
amount of folic acid on PD 2 and that the folic acid-requirement differed depending on the
anatomical region of the RMS. To our knowledge, this is the first report demonstrating the
effect of MTX on the RMS and the necessity of the folic acid metabolism on RMS development
in newborn rats. 相似文献
56.
Supermassive black hole binaries may exist in the centers of active galactic nuclei such as quasars and radio galaxies, and mergers between galaxies may result in the formation of supermassive binaries during the course of galactic evolution. Using the very-long-baseline interferometer, we imaged the radio galaxy 3C 66B at radio frequencies and found that the unresolved radio core of 3C 66B shows well-defined elliptical motions with a period of 1.05 +/- 0.03 years, which provides a direct detection of a supermassive black hole binary. 相似文献
57.
Nakamura I Obi T Sakemi Y Nakayama A Miyazaki K Ogura G Tamaki M Oka T Takase K Miyamoto A Kawamoto Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1067-1070
The prevalence of antimicrobial resistance in 128 Escherichia coli isolates was investigated in two species of invasive alien mammals (IAMs): the small Asian mongoose (SAM) and Japanese weasel (JW). The SAM is found on the main island of Okinawa, Japan, where a large number of livestock is available, and the JW is present on a small island, where is isolated from the main island, and have a small number of livestock. We focused on the two IAMs, inhabiting under the different environments, and compared their prevalence of antimicrobial-resistant E. coli. In the comparison of the frequencies of antimicrobial-resistant E. coli isolates between the SAM and JW, JW showed significantly higher prevalence of resistance against three drugs, ampicillin, chlortetracycline and nalidixic acid, compared with SAM's test results (P<0.05). The bla(TEM) gene and the aph1 gene were detected in 35 subjects (91%) of ampicillin-resistant isolates and 6 subjects (100%) of kanamycin-resistant isolates, respectively. The tet (A) gene was detected in 62 subjects (46%) of CTC-resistant isolates, and the tet (B) gene was detected in 25 subjects (8%) of those in IAM. The present results suggest that some IAMs were the carrier of antimicrobial-resistant bacteria and their genes, and the frequencies of these resistances were different between two IAM species. 相似文献
58.
Shotaro NISHIMURA Ayano SAGARA Akifumi TESHIMA Shoji TABATA 《Animal Science Journal》2011,82(6):759-763
The difference in collagen fiber content, morphological properties and hepatic stellate cell (HSC) distribution was investigated in the liver of both sexes in chicken. Collagen fiber specimens were obtained by maceration treatment with NaOH solution. HSCs were detected using desmin‐specific immunohistochemistry. The ratio of liver weight to body weight was larger in the female than the male chickens. Collagen fiber content, the numerical density of HSCs and the percentage area displaying desmin immunopositivity were not different between the right and left lobes of the liver, in both male and female chickens. However, all of these parameters were larger in the males than the females. In the light microscopic observation, many HSCs in the male had large and elongated cytoplasmic processes. Conversely, HSCs with poorly developed cytoplasmic processes were frequently observed in females. Liver tissue is structurally stronger in male chickens than females and the activity and density of HSCs may be related to the collagen fiber content in chicken liver. 相似文献
59.
60.
Hayashi S. Jinbo T. Iguchi K. Shimizu M. Shimada T. Nomura M. Ishida Y. Yamamoto S. 《Veterinary research communications》2001,25(2):117-120
The concentrations of C-reactive protein (CRP) and 1-acid glycoprotein (AAG) were evaluated in 1-, 3- and 18-month-old dogs (four of each age) that had been inoculated with turpentine oil. The CRP and AAG in 3-month-old and younger dogs subjected to surgery or inoculated with either Staphylococcus aureus or a viral vaccine were also evaluated. The average CRP concentration in the sera peaked 2 days after inoculation of turpentine oil. The peak CRP concentrations in 3- and 18-month-old dogs were significantly (p<0.05) greater than those in 1-month-old dogs. The average AAG concentration in the sera peaked 4 days after inoculation of turpentine oil. No significant difference was found in AAG concentrations between any of the age groups. When experimentally inoculated with S. aureus or subjected to oophorohysterectomy, the CRP and AAG concentrations increased in 3-month-old dogs, but they increased little in 1-month-old dogs. The CRP and AAG in dogs inoculated with the viral vaccine did not increase. In dogs with fractures or subjected to percutaneous gastrostomy, the CRP and AAG concentrations correlated with the condition of dogs. 相似文献