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排序方式: 共有146条查询结果,搜索用时 155 毫秒
91.
92.
Takeo SAKAI Ayako ISHII Takao SEGAWA Yukihiko TAKAGI Yuki KOBAYASHI Takuya ITOU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):461-465
The Flinders Technology Associates filter paper cards (FTA® cards) can be used
to store nucleic acid from various samples and are easily portable. However, RNA is
physicochemically unstable compared with DNA, and appropriate methods have not been
established for storage and extraction of RNA from FTA® cards. The present
study investigated the optimum conditions for storage and elution of viral RNA (vRNA)
using rabies virus (RABV) applied to FTA® cards. When TE buffer was used, the
elution rates of vRNA increased with the length of the elution time. When the cards were
stored at −80°C or −20°C, vRNA was stable over 3 months. Degradation of vRNAs occurred
following storage at 4°C and room temperature, suggesting that RNA should be extracted
from cards as soon as possible if no freezer is available. When we tried to amplify vRNA
from RABV-infected animal brains applied to FTA® cards and stored at −80°C for
6 months, we did not detect any amplified products with the primer set for 964 bp of RABV
N gene. However, we were able to detect amplified products by increasing the elution time
of vRNA from FTA® cards from 30 min to 24 hr or by changing the primer sets to
amplify 290 bp of N gene. Thus, we recommend extending the elution time for damaged or low
concentration samples in FTA® cards. 相似文献
93.
Yen Thi Hoang NGUYEN Nariaki NONAKA Haruhiko MARUYAMA Ayako YOSHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):671
This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection. 相似文献
94.
Morimoto M Tanimoto K Nakano S Ozaki T Nakano A Komai K 《Journal of agricultural and food chemistry》2003,51(2):389-393
The antifeedant polymethylated flavones 5-hydroxy-3,6,7,8,4'-heptamethoxyflavone, 5-hydroxy-3,6,7,8-tetramethoxyflavone, and 5,6-dihydroxy-3,7-dimethoxyflavone have been isolated from the cudweed, Gnaphalium affine D. Don (Compositae). These flavonoids and authentic analogues showed insect antifeedant activity against the common cutworm (Spodoptera litura F.). In a previous paper, it was suggested that there was no substituent on the B-ring of the flavonoid for the beneficial antifeedant activity against the common cutworm. These flavonoids having a phenyl group as the B-ring and the chromone as elimination of the B-ring from the flavonoids were used to test the hypothesis of the previously described B-ring effect. The known fact is that Sculletaria baicarensis (Rutaceae) produced the 2-phenyl flavone. Test compounds and their methylated derivatives were prepared from this material for the structure-activity relationship (SAR) study of insect antifeedant activity. In spite of the 2-phenyl flavonoids, some tested compounds did not show any insect antifeedant activity against the common cutworm, although these inactive flavonoids were deficient in the 6-substituent group on the A-ring of the flavonoid. This 6-position-substituted derivative almost showed strong insect antifeedant activity against common cutworm. Moreover, the tested flavonoids having a hydroxyl group as a substituent on any of the positions tended to increase the activity. These results suggested the importance of the 6-position substitution on the flavonoid; however, hydrophilic substituents decreased the activity. Baicalein (5,6,7-trihydroxyflavone) derivatives did not show any activity despite having the 6-substituent derivative. Although the activity of some chromones increased the activity of the flavone, the bulky B-ring was a disadvantage for the antifeedant activity. It was suggested that the charge on C(3) and C(5) of the flavonoid was important for the biological activity. Additionally, an adequate hydrogen bonding property, which is different from lipophilicity, was an advantage for the activity on the basis of a QSAR analysis. 相似文献
95.
Antioxidative and antimutagenic activities of 4-vinyl-2,6-dimethoxyphenol (canolol) isolated from canola oil 总被引:2,自引:0,他引:2
Kuwahara H Kanazawa A Wakamatu D Morimura S Kida K Akaike T Maeda H 《Journal of agricultural and food chemistry》2004,52(14):4380-4387
A potent antioxidative compound in crude canola oil, canolol, was recently identified, and reported herein are studies of its scavenging capacity against the endogenous mutagen peroxynitrite (ONOO(-)). ONOO(-) is generated by the reaction between superoxide anion radical and nitric oxide, both of which are produced by inflammatory leukocytes. Among various antioxidative substances of natural or synthetic origin, canolol was one of the most potent antimutagenic compounds when Salmonella typhimurium TA102 was used in the modified Ames test. Its potency was higher than that of flavonoids (e.g., rutin) and alpha-tocopherol and was equivalent to that of ebselen. Canolol suppressed ONOO(-)-induced bactericidal action. It also reduced intracellular oxidative stress and apoptosis in human cancer SW480 cells when used at a concentration below 20 microM under H(2)O(2)-induced oxidative stress. In addition, canolol suppressed plasmid DNA (pUC19) strand breakage induced by ONOO(-), as revealed by agarose gel electrophoresis. 相似文献
96.
Taiko Oda Jinzhong Xu Ayako Fujita Masataka Mochizuki Michio Namikoshi 《Marine drugs》2006,4(1):22-27
Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC50 = 6.8 nM). Bistheonellide A is an actin-polymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL-60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system. 相似文献
97.
To evaluate the relative importance of the factors affecting the premature abortion of acorns in Quercus serrata Thunb. ex Murray during the course of their development, inner developmental processes of acorns were observed by microscope, and applicability of the diameter of fruits (acorns with cupules) as an indicator of the inner developmental processes was discussed. Based on the observations using microscope, the developmental processes of acorns were classified into five stages; one prezygotic and four postzygotic stages (stages I to V). The diameter of sound fruits increased exponentially until 14 weeks after pollination, and the variance of diameter in each tree was fairly small (CV = 6%) during the process. Diameter of fruits can be used as a good indicator of developmental stages of acorns at individual level, and effective for the evaluation of the ecological factors affecting the premature abortion of acorns. 相似文献
98.
Osumi T Ohno K Kanemoto H Nakashima K Uchida K Karasawa A Fujino Y Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(7):937-939
A 7-year-old Miniature Dachshund presented with severe chronic jaundice and elevated liver enzymes. Destructive cholangitis was diagnosed according to histopathological findings of remarkable ductopenia with inflammatory infiltrates and fibrosis in the portal areas. Supportive therapy with prednisolone, high-dose ursodeoxycholic acid, human placental extract and antibiotics was tried, and the patient showed recovery of clinical signs 3 months after diagnosis. A second liver biopsy was performed about 1 year after initial diagnosis, and bile duct restoration was confirmed with continuous inflammation around portal areas and inside the lobules. Although we could not determine which treatment was effective in this case, destructive cholangitis in dogs may be recoverable with long-term supportive therapies. 相似文献
99.
Luteophanol D (1), a new polyhydroxyl linear carbon-chain metabolite, has been isolated from the cultured marine dinoflagellate Amphidinium sp., which was isolated from Okinawan marine acoel flatworm Pseudaphanostoma luteocoloris. The structure of 1 was elucidated by detailed analyses of 2D NMR spectra. Luteophanol D (1) possesses two tetrahydropyran rings and twenty-three hydroxyl groups on C63-linear aliphatic chain with one exo-methylene and two methyl branches. 相似文献
100.