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21.
Mie Araki Aya Numaoka Makoto Kawase Kenji Fukunaga 《Genetic Resources and Crop Evolution》2012,59(7):1303-1308
We developed PCR-based markers for genotyping Waxy genes in common millet (Panicum miliaceum L.) and proposed a new hypothesis on the origin and dispersal of waxy type of Japanese landraces. I2-K staining indicated that almost all the Japanese landraces had waxy endosperm. Based on the PCR and dCAPs analyses of two waxy loci on different genomes we concluded that there were at least two possible lineages in Asian continent that had independent dispersal pathways into Japan. Most Japanese common millet had the similar genotypes with those in Korean Peninsula but a few particularly from northern part of Japan had common genotypes in northeastern China and Primorskaya Province of Russia. 相似文献
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Toyonaga M Kaihara A Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1395-1398
On the assumption that animals of wild feline species died in the field, caudal epididymal sperm were cryopreserved following storage of the feline epididymides at 20°C for 0-24 hr, and their qualities were observed. Compared to the qualities at 0 hr, no significant differences were noted following 12 hr of storage at 20°C. On comparison of the qualities between caudal sperm cryopreserved after 24 hr storage at 4°C and after 12 hr at 20°C followed by 12 hr storage at 4°C, no significant differences were noted. These findings suggest that the cryopreserved sperm collected from epididymides of dead animals might be useful for artificial insemination if cryopreservation was performed within 12 hr exposure to ambient temperature. 相似文献
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The effects of canopy and shrub layer coverage on the frequency of oviposition by a papilionid butterfly, Luehdorfia japonica, were investigated. Density of larval host plant tended to be higher where shrub layer was thinner, and the frequency of
oviposition was correlated with host plant abundance. On the other hand, host plant abundance and the frequency of oviposition
were not correlated with canopy layer coverage. Eggs were mainly found at or near the forest edge irrespective of host plant
abundance. 相似文献
25.
Flight and avoidance reactions from human were examined using 168 postweaning
Thoroughbred foals in 22 breeding farms. Further 114 yearlings of 168 foals were tested in
the following summer. The foal handlings by the stabler were asked in questionnaire. The
relationship between the behavioural reactions and the foal handling frequencies was
analyzed. The flight reaction was estimated as the distance from the animal to a stranger
when the animal began to flight away from his approach. The avoidance scores were set up
from (1) (not resistant) to (5) (touch rejection) from human touching. In the stabler
questionnaire, handling frequencies of “body brushing”, “rectal temperature measurement”,
“hoof cleaning”, and “stall cleaning” in the early nursing period were asked. The handling
frequencies were scored from (1) (not done) to (5) (every day). In the preliminary test, a
measurement reliability of the flight distance and the avoidance score was confirmed. The
mean flight distances were 0.56 m and 0.27 m in the postweaning foals and the yearlings,
respectively. Touch-avoidance scores of the highest frequency were (3) and (2) in the
postweaning foals and the yearlings, respectively. As the results of Spearman’s
rank-correlation analysis, “body brushing” showed highly negative relationships with
“flight distance” (ρ=–0.31, P<0.001) and “avoidance score” (ρ=–0.37, P<0.001) in the
postweaning foals. In the yearlings, “hoof cleaning” also showed significantly negative
relationships with these behavioural indices (ρ=–0.24, P<0.01; ρ=–0.22, P<0.01,
respectively). 相似文献
26.
Tsuyoshi Ito Yoshitaka Katoh Yuko Shimada Aya Ohnuma-Koyama Naofumi Takahashi Maki Kuwahara Takanori Harada 《Journal of toxicologic pathology》2016,29(1):39-43
Extraskeletal osteosarcoma is extremely rare in mice. This case report demonstrates a spontaneous murine extraskeletal osteosarcoma that exhibited various histological growth patterns in an ICR mouse. At necropsy, the tumor mass was located in the abdominal wall and was 45 × 30 × 25 mm in size. Histopathologically, the tumor showed the following four growth patterns: a solid pattern of polygonal cells embedded in an osteoid eosinophilic matrix with calcification, an irregular sheet pattern of short spindle cells accompanying some eosinophilic multinucleated cells, a fascicular pattern of spindle cells and a cystic pattern lined by short spindle cells. Immunohistochemically, most of the tumor cells were positive for vimentin, proliferating cell nuclear antigen and osterix. The multinucleated cells mentioned above were desmin positive and were regarded as regenerative striated muscles but not tumor cells. Since no clear continuity with normal bone tissues was observed, the tumor was diagnosed as an “extraskeletal osteosarcoma.” 相似文献
27.
Duaa Al Qattan Amjad Shatarat Loai Alzghoul Aya Khaled Ala Abdallah Maha ELBeltagy 《Anatomia, histologia, embryologia》2019,48(5):437-443
Sexual dimorphism exists at all levels of the nervous system, from genetic, anatomical and system levels. The sexual dimorphism in the axonal content of the corpus callosum (CC) has always been controversial; hence, the aim of this study was to analyse the differences in total, myelinated and unmyelinated axons density of various regions of the CC between male and female rats. To assess that, six pairs of adult male and female rats were perfused and the CC was removed and sectioned. Four sections from different subregions of the corpus callosum that represent the genu, anterior body, posterior body, and splenium, were stained, and electron microscopic images were captured using stereological guidelines. Later, the axons density for each subregion was calculated and compared between males and females. The findings of the present study indicated region‐specific differences in the myelinated, unmyelinated or the ratio of myelinated/total axons in the CC between male and female rats. 相似文献
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Acute hypotonic stress becomes a threat to the survival of bacteria in the environment. Mechanosensitive channels play an essential role in the maintenance of bacterial cell integrity during hypoosmotic shock. A database search suggested that Campylobacter jejuni, a major worldwide cause of bacterial gastroenteritis in humans, possesses two putative mechanosensitive channels, designated Cjj0263 and Cjj1025, in C. jejuni strain 81-176. Osmotic downshock experiments demonstrated that a mutant lacking Cjj0263 showed a severe defect in survival of hypoosmotic shock, while a mutant lacking Cjj1025 exhibited the same survival capacity as the wild type. We further examined the colonization ability of each mutant using the one-day old chick model. Cjj0263 or Cjj1025 mutants were able to colonize chick ceca at the same level as the wild type, but a Cjj0263 Cjj1025 double mutant revealed significantly reduced ability to colonize chick ceca. To examine whether C. jejuni that have grown in the digestive tract of chicks are protected against acute hypotonic stress, bacteria in ceca were directly exposed to water. The wild type was able to survive acute osmotic downshift, but the Cjj0263 mutant suffered a substantial loss of viability when subjected to a rapid osmotic downshock. Immunoblot analysis suggested that both Cjj0263 and Cjj1025 were glycosylated via the N-linked protein glycosylation pathway, but glycan modification of these proteins was unlikely to have a major effect on their function and stability. Our data suggest that Cjj0263, a mechanosensitive channel, has a pivotal role in protection against hypoosmotic stress experienced during environmental transmission. 相似文献
30.
An atovaquone (ATV)-resistant Babesia gibsoni was developed by in vitro exposure of uncloned wild type (WT) B. gibsoni to 800 nM ATV for 6 days. Sequence analysis of mitochondrial genes showed a single-nucleotide polymorphism (SNP) at cytb nt363 (G to T) that resulted in the substitution of methionine with isoleucine (M121I), which is one of the SNPs reported in a previous in vivo study. 363T or 363G allele-specific real-time polymerase chain reaction (PCR) revealed that an M121I variant was present in over 99% of the ATV-resistant population. As neither ATV resistance nor gene polymorphisms appeared in the B. gibsoni WT sibling clones, the expression of ATV resistance in this study was suspected to be because of selective multiplication of the B. gibsoni M121I variant. This ATV-resistant B. gibsoni displayed the same sensitivity as the WT B. gibsoni against 5 other drugs, including diminazene aceturate, azithromycin, doxycycline, clindamycin, and proguanil. This is the first report on the in vitro establishment of an ATV-resistant B. gibsoni with gene polymorphisms. 相似文献