The final stages of dominant follicle selection in cattle

The final stages of ovarian follicle growth in cattle are typically characterized by the ultrasound-detectable emergence of a cohort of small (3-5mm in diameter) antral follicles, followed by a selection process during which the number of follicles continuing to grow decreases. Finally, only one follicle (the dominant follicle; DF) shows an enhanced growth rate and estradiol synthesis when it attains 8.5mm compared to its closest competitor (the largest subordinate follicle; SF). Cohort emergence is caused by a transient FSH rise, while DF selection occurs during declining FSH indicating differential FSH dependence of DF and SF. In order to elucidate the mechanisms underlying DF survival or SF atresia, this review aims to (i) describe follicular changes in the local production and regulation of members of the inhibin family of proteins and the insulin-like growth factor (IGF) system in relation to FSH deprivation leading to DF selection, and (ii) develop a model for DF selection outlining the putative involvement of inhibins, activin and follistatin on the one hand, and bioavailable IGFs regulated by IGF binding proteins (IGFBPs) and IGFBP proteases on the other hand. It is concluded, that the first indications of differential FSH dependence are seen within 33h of the FSH peak, and high amounts of precursor forms of inhibin and free activin, and low amounts of the lower molecular weight (MW) IGFBPs are related to follicle survival in terms of enhanced growth and estradiol synthesis, and suppression of granulosa cell apoptosis. In addition, maintenance of low amounts of intrafollicular IGFBP4 may constitute an important mechanism in the future DF to attain FSH independence.     

Aspects of the metabolism of dairy cows during the incubation of bovine spongiform encephalopathy

As part of a nutritional study lasting from six weeks before calving to 22 weeks of lactation, blood samples collected from 47 dairy cows maintained under well-defined conditions were analysed for a variety of metabolites and hormones. At various times after the completion of the study, six of the animals developed clinical signs of bovine spongiform encephalopathy (BSE), although when they were sampled it was not known that they were incubating the disease. The data were used to make comparisons between the animals that developed BSE and those that did not develop the disease and which had been maintained under the same conditions. The greatest differences between the animals incubating BSE and the control animals were observed at times of nutritional stress, at the start of lactation and when the intake of concentrate feeds was reduced at week 13 of lactation. In the animals that subsequently developed BSE, feed intakes were lower in early lactation; plasma beta-hydroxbutyrate concentrations were significantly higher (P<0.001) at weeks 3 and 5 of lactation; adjusted milk yields were lower until week 6 of lactation and milk fat concentrations were consistently lower. There was no effect on plasma glucose concentrations, although insulin concentrations were significantly lower in week 1 of lactation (2-27 v 2.50 microiu/ml) (P<0.05). The concentrations of plasma proteins and urea were unaffected by BSE incubation, apart from protein concentrations being significantly higher one week before calving, and the concentration of urea being significantly lower five weeks before calving. The plasma concentrations of somatotrophin, prolactin, oestradiol and progesterone were similar in the two groups of animals throughout the study. The differences observed indicate that the energy metabolism of dairy cows incubating BSE may be subtly altered before the onset of clinical signs of the disease.     

One-day rate measurements for estimating net nitrification potential in humid forest soils

Measurements of net nitrification rates in forest soils have usually been performed by extended sample incubation (2–8 weeks), either in the field or in the lab. Because of disturbance effects, these measurements are only estimates of nitrification potential and shorter incubations may suffice. In three separate studies of northeastern USA forest soil surface horizons, we found that laboratory nitrification rates measured over 1 day related well to those measured over 4 weeks. Soil samples of Oa or A horizons were mixed by hand and the initial extraction of subsamples, using 2 mol L⁻¹ KCl, occurred in the field as soon as feasible after sampling. Soils were kept near field temperature and subsampled again the following day in the laboratory. Rates measured by this method were about three times higher than the 4-week rates. Variability in measured rates was similar over either incubation period. Because NO₃⁻ concentrations were usually quite low in the field, average rates from 10 research watersheds could be estimated with only a single, 1-day extraction. Methodological studies showed that the concentration of NH₄⁺ increased slowly during contact time with the KCl extractant and, thus, this contact time should be kept similar during the procedure. This method allows a large number of samples to be rapidly assessed.     

Allozyme diversity in Australian rainbow trout, Oncorhynchus mykiss

Rainbow trout, Oncorhynchus mykiss (Walbaum), were first introduced into Australia over 100 years ago, and forms the basis of important recreational inland fisheries and an aquaculture industry in south‐eastern Australia. This paper investigates the genetic variation within and between samples of Australian rainbow trout using allozyme electrophoresis. The levels of genetic diversity within Australia do not show marked differences from those observed in hatchery and wild populations from throughout North America, New Zealand and South Africa, but there is evidence for the loss of some rare alleles during translocation from California to Australia via New Zealand. No appreciable difference in genetic diversity was apparent between hatchery and self‐sustaining wild populations of rainbow trout from mainland Australia. However, significant differences in allelic frequencies were observed, with consistent genetic differences between Victorian and New South Wales samples most likely reflecting state‐based hatchery and stocking policies.     

A Large‐scale,Rapid Public Health Response to Rabies in an Organ Recipient and the Previously Undiagnosed Organ Donor

This article describes and contrasts the public health response to two human rabies cases: one organ recipient diagnosed within days of symptom onset and the transplant donor who was diagnosed 18 months post‐symptom onset. In response to an organ‐transplant‐related rabies case diagnosed in 2013, organ donor and recipient investigations were conducted by multiple public health agencies. Persons with potential exposure to infectious patient materials were assessed for rabies virus exposure. An exposure investigation was conducted to determine the source of the organ donor's infection. Over 100 persons from more than 20 agencies spent over 2700 h conducting contact investigations in healthcare, military and community settings. The 564 persons assessed include 417 healthcare workers [5.8% recommended for post‐exposure prophylaxis (PEP)], 96 community contacts (15.6% recommended for PEP), 30 autopsy personnel (50% recommended for PEP), and 21 other persons (4.8% recommended for PEP). Donor contacts represented 188 assessed with 20.2% recommended for PEP, compared with 5.6% of 306 recipient contacts recommended for PEP. Human rabies cases result in substantial use of public health and medical resources, especially when diagnosis is delayed. Although rare, clinicians should consider rabies in cases of encephalitis of unexplained aetiology, particularly for cases that may result in organ donation.     

Use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, Oncorhynchus mykiss (Walbaum)

From a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, Oncorhynchus mykiss, and carp, Cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by Lactococcus garvieae and Streptococcus iniae when used as a feed additive. The culture, Aeromonas sobria GC2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. Whereas the untreated controls experienced losses of 75-100% when challenged intraperitoneally with L. garvieae and S. iniae, the probiotic-treated groups remained healthy with total mortalities of only 0-6%. Formalized and sonicated preparations of GC2 and cell-free supernatant fared less well. The mode of action reflected stimulation of innate immunity, namely an increased number of leucocytes and enhanced phagocytic and respiratory burst activity.     

Associations between grain yield and carbon isotope discrimination in cowpea

Twenty cowpea (Vigna unguiculata L. Walp.) genotypes, comprising Kenyan cultivars and Kenyan and introduced breedings lines, were grown in 15 replicated field trials carried out at three locations in semi-arid eastern Kenya over four seasons. Grain and straw yields and the carbon isotope discrimination (Δ) of this material were determined, and days to flowering and maturity observed.None of the seasons in which the trials were conducted was appreciably drier than the long term average for the sites, and some trials received excessive rain. Averaging the data for the 15 trials, one genotype yielded much less grain (77 g m⁻²) than the 19 others, whose yield ranged from 125 to 177 g m⁻² (average 148 g m⁻²). Trial mean yield trials had a high straw Δ (r = +0.567) and had received more rain between flowering and maturity (r = +0.428) than lower yielding ones. Among genotypes, averaging over trials, the correlation coefficient (r) between grain yield and grain Δ was +0.394, and between grain yield and straw Δ, +0.460. Early genotypes had the highest grain Δ and straw Δ. The correlation among genotypes between date of flowering and grain Δ was −0.632 and with straw Δ, −0.502. When comparisons were made among trials, there was no clear relationship between the strength of the correlations among genotypes between grain yield and straw Δ or grain Δ and the degree of stress experienced by a trial (as indicated by the rainfall it had received). It appeared that this was because the variances of grain Δ and straw Δ were greater in the most droughted trials, whereas the variance of yield was greatest in the least droughted trials.It is concluded that the genetic correlation between grain yield and either grain Δ or straw Δ is unlikely to be strong enough to make either Δ a useful surrogate or adjunct in selecting for high yield in the segregating generations of a breeding programme for semi-arid eastern Kenya, but that Δ may be of value in the selection of parent genotypes.