大袋蛾性信息素立体构型的确定

对合成的大袋蛾性信息素的4种立体异构体进行林间诱捕试验,确定了其具有强活性的立体构型为(3R,13R)-(1S)- 1-乙基-2-甲丙基 3,13-二甲基十五酸甲酯.     

Partial substitution of dietary fish meal with soybean meal for speckled shrimp,Metapenaeus monoceros (Fabricius, 1798) (Decapoda: Penaeidae) juvenile

A 63‐day feeding study was conducted to evaluate the effect of partial substitution (0%, 20%, 40% and 60%) of dietary fish meal (FM) with soybean meal (SBM) on the growth performance and feed utilization of speckled shrimp, Metapenaeus monoceros juveniles. A total of 180 M. monoceros juveniles with an average weight of 0.67 ± 0.18 g were equally distributed over 12 glass aquaria (30 L each, triplicate per treatment). All aquaria were filled with filtered sea water (30–35 ‰, salinity). Four isonitrogenous (400 g kg^?1 crude protein) and isocaloric (20.5 MJ GE kg^?1) test diets were formulated. The control diet contained FM (D₁) as the main protein source. In the other diets, FM was partially replaced with 20 (D₂), 40% (D₃) or 60% (D₄) SBM. The results indicate that the diet in which 40% of the FM is replaced by SBM yields similar growth and production, and is as cost effective, as the control diet.     

Effects of dietary marine microalgae,Tetraselmis suecica,on production,gene expression,protein markers and bacterial count of Pacific white shrimp Litopenaeus vannamei

The marine flagellated Chlorophyta Tetraselmis suecica is among the most important live food species in marine aquaculture. In the present study, the effects of dietary supplementation of dried marine microalgae, Tetraselmis suecica, on growth performance; feed utilization; chemical composition; gene expression of superoxide dismutase (SOD), glutathione peroxidase (GPx) and insulin‐like growth factor 2 (IGF‐II) gene of Pacific white shrimp, Litopenaeus vannamei; muscle protein polymorphism; and microbial count were assessed and evaluated. Three hundred and sixty L. vannamei (postlarvae) Pls (0.124 ± 0.002 g) were randomly stocked into 40‐L glass aquaria (30 shrimp/aquarium) and fed three times daily four tested diets: a basal diet (control), diet incorporated with 2.5 g kg^?1 dried T. suecica (T1), 5 g kg^?1 dried T. suecica (T2) and 7.5 g kg^?1 dried T. suecica (T3) in triplicates, for 90 days. At the end of the trial, the survival rate (SR) of L. vannamei fed diets supplemented with different levels of T. suecica was significantly (p < .05) higher than the control diet. The highest weight gain and specific growth rate and the best feed conversion ratio were recorded on L. vannamei fed a diet supplemented with a 7.5 g/kg dried T. suecica. The highest protein, lipid and ash contents were obtained in L. vannamei fed the diet containing 7.5 g/kg T. suecica, when compared with the remaining tested diets. The gene expression of antioxidant genes SOD and GPx was the lowest in the T3 group in comparison with the control group. Meanwhile, expression level of IGF‐II was higher in the T2 group. The total heterotrophic bacterial count was significantly (p < .05) increased with the cumulative T. suecica level, while no significant (p > .05) differences were found in the total Vibrio count among treatments. Overall, the present results have shown that the diet supplemented with the highest inclusion level of dried T. suecica resulted in improved growth and nutrient utilization.     

Detection of antibodies against serotypes 1 and 2 infectious bursal disease virus by commercial ELISA kits

Two distinct serotypes of infectious bursal disease virus (IBDV) are recognized in chicken and turkey flocks in the United States. Serologic testing of chicken flocks for serotype 1 viruses is routinely performed to monitor disease status and vaccination. Earlier studies indicated that enzyme-linked immunosorbent assay (ELISA) test detects antibodies to both serotypes of the virus, while the virus neutralization (VN) test is serotype specific. It is useful to evaluate currently available commercial ELISA kits for their ability to differentiate between antibodies elicited by the two serotypes. Three trials were performed in which chickens were orally inoculated with either a high or a low dose of serotype 1 STC or serotype 2 OH strains of IBDV. Sera collected at 0, 7, 14, and 21 days from these chickens and antisera procured from naturally infected broiler (n=20) and layer (n=30) flocks were tested with five different commercial ELISA kits and by VN. All ELISA kits detected different levels of antibodies elicited against serotype 1 of the virus and moderate and high levels of antibodies against serotype 2 virus. A correlation existed between the ELISA and the VN titers of experimentally infected chickens. All serum samples tested from the commercial layer flocks and 65% of the broiler flocks had antibodies against the OH strain. However, no correlation between the VN titers and ELISA titers was observed for the commercial broilers and layers sera by the majority of the kits. The results indicated that currently available commercial ELISA kits detect antibodies elicited by the two serotypes of IBDV. Hence, the prevalence of serotype 2 antibodies in the flocks should be considered while determining antibody profiles of the flocks against serotype 1 viruses.     

Heterospectral two-dimensional correlation spectroscopy of mid-infrared and Fourier self-deconvolved near-infrared spectra of sugar solutions

The mid- and near-infrared (mid-IR and NIR) spectra of aqueous solutions of glucose and fructose, fructose and galactose, and glucose and galactose were recorded and analyzed by heterospectral two-dimensional correlation spectroscopy (H2D-CS) to determine characteristic NIR wavelengths for each sugar. Fourier self-deconvolution (FSD) was applied to the NIR spectra prior to H2D-CS analysis to help resolve the strongly overlapping sugar absorptions. Examination of the H2D-CS data gave characteristic absorption wavelengths for glucose, fructose, and galactose. The wavelengths identified by H2D-CS were then used to develop multiple linear regression (MLR) calibrations for the quantitative analysis of mixtures of the three sugars in solution. This approach gave comparable results to MLR calibrations based on wavelengths selected by examination of the first- and second-derivative spectra of solutions of the individual sugars.     

Synergy of remotely sensed data in spatiotemporal dynamic modeling of the crop and cover management factor

Soil erosion is a threat to the water quality constituents of sediments and nutrients and can cause long-term environmental damages. One important parameter to quantify the risk of soil loss from erosion is the crop and cover management factor (C-factor), which represents how cropping and management practices affect the rates and potential risk of soil erosion. We developed remotely sensed data-driven models for dynamic predictions of C-factor by implementing dynamic land cover modeling using the SWAT (Soil and Water Assessment Tool) model on a watershed scale. The remotely sensed processed variables included the enhanced vegetation index (EVI), the fraction of photosynthetically active radiation absorbed by green vegetation (FPAR), leaf area index (LAI), soil available water content (AWC), slope gradient (SG), and ratio of area (AR) of every hydrologic response unit (HRU) to that of the total watershed, comprising unique land cover, soil type, and slope gradient characteristics within the Fish River catchment in Alabama, USA between 2001 and 2014. Linear regressions, spatial trend analysis, correlation matrices, forward stepwise multivariable regression (FSMR), and 2-fold cross-validation were conducted to evaluate whether there were possible associations between the C-factor and EVI with the successive addition of remotely sensed environmental factors. Based on the data analysis and modeling, we found a significant association between the C-factor and EVI with the synergy of the environmental factors FPAR, LAI, AWC, AR, and SG (predicted R² (R_pred²) = 0.51; R² = 0.68, n = 3 220, P < 0.15). The results showed that the developed FSMR model constituting the non-conventional factors AWC (R_pred² = 0.32; R² = 0.48, n = 3 220, P < 0.05) and FPAR (R_pred²= 0.13; R² = 0.28, n = 3 220, P = 0.31) was an improved fit for the watershed C-factor. In conclusion, the union of dynamic variables related to vegetation (EVI, FPAR, and LAI), soil (AWC), and topography (AR and SG) can be utilized for spatiotemporal C-factor estimation and to monitor watershed erosion.     

MODULATION OF PHYSIOLOGICAL AND BIOCHEMICAL METABOLITES IN SALT STRESSED RICE BY FOLIAR APPLICATION OF ZINC

An experiment was conducted to evaluate the effect of zinc (Zn) application on five rice cultivars grown under salt stress conditions. Two salinity levels of 0 and 10 dS m^?1 were created with sodium chloride (NaCl) and foliar spray of Zn (0.05%; Chelated-Zn) was applied. A decrease in growth and yield related parameters were observed under salt stress, which was ameliorated in plants that received Zn foliar spray. Similar effects of salinity and Zn foliar spray were noted on photosynthetic rate, transpiration rate, stomatal conductance, water use efficiency, and water relations of plants. Salt induced increase in sodium (Na) content and decrease in other macro- and micronutrients contents were also reversed by Zn. Other salt tolerance indicating parameters likSe total free amino acids and total soluble sugars increased under Zn spray, clearing its role in improving salt tolerance.     

Dry Matter and Nitrogen Distribution at Maturity of Three Rice (Oryza sativa L.) Cultivars Exposed to Ammonia at Two Growth Stages

A substantial proportion of ammonical fertilizers applied to lowland rice is lost as gaseous N from the soil–plant system. Besides various environmental factors, the low N use efficiency of flooded rice is also attributed to this factor. As atmospheric ammonia found in the leaf environment of the plants could also be responsible for differences in N use efficiency and fertilizer N losses from lowland rice, a greenhouse study was conducted on three rice cultivars varying in physio-morphological characteristics for their dry matter and nitrogen distribution to grains at maturity in response to ammonia (NH₃) exposure at tillering and anthesis growth stages. The results revealed that ammonia exposure of plants at two growth stages did not affect the total dry matter and total N yield of the rice cultivars at maturity; however, the grain yield and grain N yield were negatively effected by NH₃ exposure of the plants at anthesis. The variation observed in dry matter and N partitioning at maturity to grains/roots of the plants exposed to NH₃ at anthesis indicated that the growth stage of the plants at which they are exposed to NH₃ has an influence on N use efficiency of crop plants and subsequent vegetative as well as total N losses from the soil–plant system.     

Responses of Some Local/Exotic Accessions of Lentil (Lens culinaris Medic.) to Salt Stress

Assessment of salt tolerance at all growth stages is crucial to determine the overall tolerance of a crop. Salt tolerance of five tolerantILL 5845, ILL 6451, ILL 6788, ILL 6793 andILL 6796, three moderately tolerant ILL 6431. ILL 6770 andILL 6784, and three sensitiveILL 6210, ILL 6439 andILL 6778 accessions selected at the germination and seedling stages was assessed at the adult stage using sand culture sahnized with 0, 30, or 60 mol m^?3 NaCl. A positive correlation was observed between degrees of salt tolerance at different growth stages in three tolerant accessionsILL 6451, ILL 6788 andILL 6793 which produced significantly higher seed yield than the other accessions. This was also affirmed in three sensitive and two moderately tolerant accessions (ILL 6770 andILL 6784) whose salt sensitivity was conferred consistently at all growth stages. In contrastILL 5845, andILL 6796 which were highly salt tolerant andILL 6431 which was moderately tolerant at the early growth stages had relatively low seed yield, hence showing a negative correlation between tolerances at different growth stages. High yielding accessionsILL 6451, ILL 6788 andILL 6793 in general, accumulated higher Na⁺ and higher or moderate Cl^? in their shoots compared with the other accessions, thus showing a typical halophytic mechanism of salt inclusion. K/Na ratios of all the tolerant, moderately tolerant and sensitive accessions exceptILL 6784 andILL 6778 were less than 1, a suggested minimum level for normal functioning of many metabolic processes in plants. The present study shows that salt tolerance observed previously at the early growth stages is conferred at the adult stage in most of the accessions of lentil examined here; but for others in which no positive correlation was observed between different growth stages suggests that a combination of certain characters can be used as selection criterion for improving salinity tolerance in lentil through exploitation of inter- and intra-cultivar/line variation.     

Does Salt Tolerance Vary in a Potential Oil-Seed Crop Brassica carinata at Different Growth Stages?

The salt tolerance of two salt-tolerant (C90-1191 and P5/80) and two salt-sensitive (C90-1115 and 77–321) lines of Ethiopian mustard ( Brassica carinata ) (selected on the basis of their performance in germinating ability and seedling growth in a screening experiment) was assessed at the adult stage under glasshouse conditions to evaluate the consistency of salt tolerance at different growth stages of plant life cycle. No difference in response of all four lines differing in salt tolerance was found to varying concentrations of NaCl in the growth medium, although the latter two lines were slightly better than the former two in shoot dry mass, seed yield or yield components. The relatively good growth of the lines C90-1115 and 77-321 at the adult stage can be related to their lower accumulation of Na, Cl and Ca in their leaves. K/Na ratios, Ca/Na ratios, K versus Na selectivity, and Ca versus Na selectivity did not prove to be successful criteria in discriminating the lines differing in degree of salt tolerance. In conclusion, salt tolerance in B. carinata varies with the change in stage of its life cycle. Thus selection based at one particular stage may not produce individuals tolerant at all growth stages.