Effect of oral melatonin administration on sex hormone, prolactin, and thyroid hormone concentrations in adult dogs.

OBJECTIVE: To determine the effect of oral melatonin (MT) administration on serum concentrations of sex hormones, prolactin, and thyroxine in dogs. DESIGN: Prospective study. ANIMALS: 8 male and 8 female adult sexually intact dogs. PROCEDURE: 5 male and 5 female dogs were treated with MT (1.0 to 1.3 mg/kg [0.45 to 0.59 mg/lb] of body weight), PO, every 12 hours for 28 days; the other 6 dogs were used as controls. Blood samples were collected on days 0, 14, and 28, and serum concentrations of estradiol-17 beta, progesterone, testosterone, androstenedione, 17-hydroxyprogesterone (17-HP), dihydroepiandrostenedione sulfate (DHEAS), prolactin, and thyroxine were determined. On day 5, serum MT concentrations were measured before and periodically for up to 8 hours after MT administration in 4 treated dogs. RESULTS: Female dogs treated with MT had significant decreases in serum estradiol, testosterone, and DHEAS concentrations between days 0 and 28. Male dogs treated with MT had significant decreases in serum estradiol and 17-HP concentrations between days 0 and 28. Serum MT concentrations increased significantly after MT administration and remained high for at least 8 hours. Prolactin and thyroxine concentrations were unaffected by treatment. CONCLUSIONS AND CLINICAL RELEVANCE: Melatonin is well absorbed following oral administration and may alter serum sex hormone concentrations.     

Developing Tools to Encourage Private Forest Landowners to Participate in Early Successional Forest Habitat Management

Wildlife organizations often engage landowners in habitat management. Landowner typology research can provide suggestions for how to work with diverse types of landowners. We explored how typologies can inform selection of tools to engage landowners in early successional habitat (ESH) management. Using a survey, effectiveness of three kinds of tools were assessed: (a) basic needs, (b) learning, and (c) social. Across all types and typologies, learning tools were most likely to influence landowner behavior, whereas social tools (e.g., recognition) were least likely. Continuing adopters were the only type of landowners that reported basic needs tools would influence them at the same level as learning tools. In each typology, landowner types with higher behavioral intention were more likely to be influenced by all of the tools. Thus, tools may reinforce behaviors, rather than engage the unengaged. We suggest learning tools be initially prioritized to encourage ESH management.     

The spatial periodicity of grid cells is not sustained during reduced theta oscillations

Grid cells in parahippocampal cortices fire at vertices of a periodic triangular grid that spans the entire recording environment. Such precise neural computations in space have been proposed to emerge from equally precise temporal oscillations within cells or within the local neural circuitry. We found that grid-like firing patterns in the entorhinal cortex vanished when theta oscillations were reduced after intraseptal lidocaine infusions in rats. Other spatially modulated cells in the same cortical region and place cells in the hippocampus retained their spatial firing patterns to a larger extent during these periods without well-organized oscillatory neuronal activity. Precisely timed neural activity within single cells or local networks is thus required for periodic spatial firing but not for single place fields.     

Land cover and climate changes drive regionally heterogeneous increases in US insecticide use

Context

Global environmental change is expected to dramatically affect agricultural crop production through a myriad of pathways. One important and thus far poorly understood impact is the effect of land cover and climate change on agricultural insect pests and insecticides.

Objectives

Here we address the following three questions: (1) how do landscape complexity and weather influence present-day insecticide use, (2) how will changing landscape characteristics and changing climate influence future insecticide use, and how do these effects manifest for different climate and land cover projections? and (3) what are the most important drivers of changing insecticide use?

Methods

We use panel models applied to county-level agriculture, land cover, and weather data in the US to understand how landscape composition and configuration, weather, and farm characteristics impact present-day insecticide use. We then leverage forecasted changes in land cover and climate under different future scenarios to predict insecticide use in 2050.

Results

We find different future scenarios—through modifications in both landscape and climate conditions—increase the amount of area treated by ~ 4–20% relative to 2017, with regionally heterogeneous impacts. Of note, we report large farms are more influential than large crop patches and increased winter minimum temperature is more influential than increased summer maximum temperature. However, our results suggest the most important determinants of future insecticide use are crop composition and farm size, variables for which future forecasts are sparse.

Conclusions

Both landscape and climate change are expected to increase future insecticide use. Yet, crop composition and farm size are highly influential, data-poor variables. Better understanding of future crop composition and farm economics is necessary to effectively predict and mitigate increases in pesticide use.

     

Land-use legacy of historical tree harvesting for charcoal production in a semi-arid woodland

Historical processes may have longer lasting effects in arid forest and woodlands because of slow rates of succession. We investigated the influence of historical tree harvesting upon contemporary spatial dynamics of pinyon-juniper woodland in the western United States. Despite intensive and widespread harvesting of woodland by the charcoal industry during the late 19th century, the role of afforestation has seldom been addressed as a potential mechanism for the dramatic expansion of pinyon-juniper woodlands over the past century. Spatial models of disturbance processes are one way to ascertain effects of such historical influences. We developed a process-based spatial model to estimate the historical tree harvest pressure according to a parsimonious set of rules and constraints. The model used a convection-diffusion approach that incorporated the influences of topography, transportation, and mining production. We used a combination of field-surveyed, archaeological, historical, and GIS data sets to calibrate, validate, and compare the alternative mathematical models. Model results were consistent with the historical harvest evidence (AUC > 0.66), with high harvest intensity closer to the mining districts, quickly decreasing at the maximum distance of influence. Performance was improved by including the local terrain. Harvesting initially showed radial patterns emanating from the significant mining districts, and then gradually expanded northward with development of the railway system. Our spatial modeling approach provides a means to assess the pattern and magnitude of historical tree harvesting in semi-arid woodlands. The general approach can be applied to explore the importance of other historical disturbance and cultural processes, whose effects may no longer be evident, in forests and woodlands worldwide.     

Intertidal Soundscapes of Hardened and Living Shorelines: A Case Study of Habitat Enhancement

相似文献   

Application of analytical standards in method development: selected chlorinated phenols and herbicides

Pure analytical standards are key components in the successful development of accurate analytical methods. When such compounds are unavailable commercially, a laboratory synthesis activity can provide such materials for use in method development. An example of such an approach is the synthesis of ethyl ethers of chlorinated phenols and ethyl esters of several phenoxy herbicides. Individual synthesis steps and the characterization of such materials are described. The use of such analytical standards in method development is vital for defining method characteristics. In addition, such materials are helpful in diagnosing chromatographic and mass spectral problems in the course of this work. Key mass spectral information including parent and daughter scans obtained from these analytical standards by using tandem mass spectrometry provide the basis for a new analytical method with increased sensitivity and specificity.