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71.
72.
A.M. Prados-Ligero J.L. González-Andújar J.M. Melero-Vara M.J. Basallote-Ureba 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(9):861-870
Pseudothecia of Pleospora allii developed best on garlic leaf debris infected by Stemphylium vesicarium incubated at low temperature (5–10°C) and relative humidity (RH) close to saturation. RH of less than 96% prevented the formation of pseudothecia, while an incubation temperature of 15–20°C led to the early degeneration of pseudothecia. Under natural conditions, colonization by pseudothecia of unburied garlic leaf debris varied between seasons from 6.0 to 15.5 pseudothecia/mm2, whereas lower colonization levels were recorded when samples were buried. Pseudothecial maturity was reached 1–4mo after the deposition of garlic debris on the soil surface and 15 days after the burial of residues. In the later case, pseudothecia degenerated with degradation of the plant debris. Ascospore release, which required rainfall or dew periods, occurred between late January and late April depending upon the year. A high correlation was found between pseudothecia maturation and four meteorological variables. Two of which, i.e. the number of hours with RH98% and with a mean temperature of 4.5–10.5°C, and the accumulated rainfall, explained most variability (adjusted R2=0.82–0.98 depending upon the year). A multiple regression equation relating the pseudothecia maturity index with these two variables could be used to forecast the epidemic onset of Stemphylium leaf spots in Southern Spain. Temporal progress of pseudothecia maturation was best fitted by a monomolecular model. 相似文献
73.
Gyula Pinke Péter Karácsony Zoltán Botta-Dukát Bálint Czúcz 《Journal of pest science》2013,86(3):621-631
The weed control of sunflower is a great challenge for farmers throughout the World. In Hungary, one of the greatest concerns is the pernicious weed Ambrosia artemisiifolia, which produces allergenic pollen. The main goal of this study was to identify cultural, weed-management and environmental factors determining weed species composition and the abundance of A. artemisiifolia in sunflower fields. Altogether 49 sunflower fields across Hungary were surveyed for their weed flora, and 30 environmental, cultural and weed-management factors were measured. Using a minimal adequate model containing 14 terms, 38 % of the total variation in species data could be explained. Soil Mg and Ca content, preceding crop, temperature, and field size had significant effects on species composition. Most of the herbicides were effective against annual grass species, but no herbicide was universally effective against broad-leaved weeds. Almost all types of weeds were efficiently reduced with mechanical weed control. A relatively high share of the explanatory variables were environmental factors, suggesting that the success of weed management in sunflower fields strongly depends on a complex of edaphic and climatic constraints. The abundance of Ambrosia artemisiifolia was positively correlated with high soil Ca content, lower temperature, the preceding crop being a cereal, and smaller field sizes; while considering herbicides it seemed to be most sensitive to fluorchloridon and propisochlor application. To reduce noxious broad-leaved weed species could require specific herbicide mixtures, and mechanical weed control should also be integrated into weed management. 相似文献
74.
Effect of cyclodextrin diallyl maleate on methane production, ruminal fermentation and microbes in vitro and in vivo 总被引:1,自引:0,他引:1
Zeenat Ara LILA Nazimuddin MOHAMMED Noriko TATSUOKA Shuhei KANDA Yuzo KUROKAWA Hisao ITABASHI 《Animal Science Journal》2004,75(1):15-22
Effects of β‐cyclodextrin diallyl maleate (CD‐M) on methane production, ruminal fermentation and digestibility were studied both in vitro and in vivo. In in vitro study, diluted ruminal fluid (30 mL) was incubated anaerobically at 38°C for 6 and 24 h with or without CD‐M using hay plus concentrate (1.5:1) as a substrate. The CD‐M was added at different concentrations (0, 1.25, 2.5, 5.0 and 7.5 g/L). The pH of the medium and numbers of protozoa were not affected by the addition of CD‐M. Total volatile fatty acids were increased and ammonia‐N was decreased, molar proportion of acetate was decreased and propionate was increased (P < 0.05) by CD‐M. Methane was inhibited (P < 0.05) by 14–76%. The effect of CD‐M on methane production and ruminal fermentation was further investigated in vivo using four Holstein steers in a cross‐over design. The steers were fed Sudangrass hay and concentrate mixture (1.5:1) with or without CD‐M (2% of feed dry matter) as a supplement. Ruminal proportion of acetate tended to decrease and that of propionate was increased (P < 0.05) 2 h after CD‐M dosing. Total viable counts, cellulolytic, sulfate reducing, acetogenic bacteria and protozoa were unaffected while methanogenic bacteria were decreased (P < 0.05) by CD‐M. The plasma concentration of glucose was increased, whereas that of urea‐N was decreased (P < 0.05). Methane was inhibited (P < 0.05) from 36.4 to 30.1 L/kg dry matter intake by the addition of CD‐M. Apparent digestibilities of dry matter and neutral detergent fiber were not affected while that of crude protein was increased (P < 0.05) in the medicated steers. These data suggested that dietary supplementation of CD‐M decreased methane production and improved nutrient use. 相似文献
75.
Electro-acupuncture and Chinese herbs for treatment of cervical intervertebral disk disease in a dog
Ayne Murata Hayashi Júlia Maria Matera Tatiana Soares da Silva Ana Carolina Brand?o de Campos Fonseca Pinto Sílvia Renata Gaido Cortopassi 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):95-98
A non-ambulatory dog with tetraparesis following a pain episode that had evolved over 2 months was submitted for medical treatment and diagnosed with intervertebral disk disease at C3-C4 and dorsal extradural compression at C1-C2 and C3-C4 using myelography and computed tomography. The dog experienced ambulation recovery after 15 days of treatment with only electroacupuncture and Chinese herbal medicine, with marked improvement occurring after only 10 treatments. Six months of follow-up demonstrated that the dog was stable and had no recurrence of symptoms. Therefore, it was concluded that the combination of electroacupuncture and Chinese herbal medicine was responsible for motor rehabilitation. 相似文献
76.
Winfried Schröder Stefan Nickel Simon Schönrock Roman Schmalfuß Werner Wosniok Michaela Meyer Harry Harmens Marina V. Frontasyeva Renate Alber Julia Aleksiayenak Lambe Barandovski Oleg Blum Alejo Carballeira Maria Dam Helena Danielsson Ludwig De Temmermann Anatoly M. Dunaev Barbara Godzik Katrin Hoydal Zvonka Jeran Gunilla Pihl Karlsson Pranvera Lazo Sebastien Leblond Jussi Lindroos Siiri Liiv Sigurður H. Magnússon Blanka Mankovska Encarnación Núñez-Olivera Juha Piispanen Jarmo Poikolainen Ion V. Popescu Flora Qarri Jesus Miguel Santamaria Mitja Skudnik Zdravko Špirić Trajce Stafilov Eiliv Steinnes Claudia Stihi Ivan Suchara Lotti Thöni Hilde Thelle Uggerud Harald G. Zechmeister 《Annals of Forest Science》2017,74(2):31
Key message
Moss surveys provide spatially dense data on environmental concentrations of heavy metals and nitrogen which, together with other biomonitoring and modelling data, can be used for indicating deposition to terrestrial ecosystems and related effects across time and areas of different spatial extension.Context
For enhancing the spatial resolution of measuring and mapping atmospheric deposition by technical devices and by modelling, moss is used complementarily as bio-monitor.Aims
This paper investigated whether nitrogen and heavy metal concentrations derived by biomonitoring of atmospheric deposition are statistically meaningful in terms of compliance with minimum sample size across several spatial levels (objective 1), whether this is also true in terms of geostatistical criteria such as spatial auto-correlation and, by this, estimated values for unsampled locations (objective 2) and whether moss indicates atmospheric deposition in a similar way as modelled deposition, tree foliage and natural surface soil at the European and country level, and whether they indicate site-specific variance due to canopy drip (objective 3).Methods
Data from modelling and biomonitoring atmospheric deposition were statistically analysed by means of minimum sample size calculation, by geostatistics as well as by bivariate correlation analyses and by multivariate correlation analyses using the Classification and Regression Tree approach and the Random Forests method.Results
It was found that the compliance of measurements with the minimum sample size varies by spatial scale and element measured. For unsampled locations, estimation could be derived. Statistically significant correlations between concentrations of heavy metals and nitrogen in moss and modelled atmospheric deposition, and concentrations in leaves, needles and soil were found. Significant influence of canopy drip on nitrogen concentration in moss was proven.Conclusion
Moss surveys should complement modelled atmospheric deposition data as well as other biomonitoring approaches and offer a great potential for various terrestrial monitoring programmes dealing with exposure and effects.77.
In order to evaluate the protection conferred by an experimental inactivated vaccine against infectious coryza, three challenge trials were undertaken using 112 1-day-old broilers. The vaccine "Hepa Inmuno NC" included bacterial antigens of Avibacterium paragallinarum (serogroups A, B, variant B, and C) as well as antigens of Newcastle virus and hepatitis virus. Fifty-six broiler chicks were vaccinated at the first day of life at the hatchery while another 56 chicks were left unvaccinated. Three infection trials were conducted simultaneously using each of the three serogroups A, B, or C of Av. paragallinarum. In each trial, 17 vaccinated and 17 unvaccinated broilers were used. Challenge was performed at day 31 of life by injection, into the left infraorbital sinus, of approximately 1 x 10(5) colony forming units of the corresponding Av. paragallinarum strain. Clinical signs were recorded on day 2 postchallenge. All broilers were euthanatized and both infraorbital sinuses were bacteriologically examined for the presence of Av. paragallinarum on day 5 postchallenge. In comparison with the unvaccinated broilers, the vaccine significantly reduced the number of broilers with clinical signs after challenge with serogroup B, and significantly fewer vaccinated broilers were positive for the presence of Av. paragallinarum after challenge with serogroup C. On the other hand, no significant protection was observed when broilers were challenged with Av. paragallinarum from serogroup A. Despite the high infection rates in vaccinated chicks after an experimental infection with Av. paragallinarum, it was possible to reduce colonization of Av. paragallinarum (serogroup B) and clinical signs (serogroup C) in broiler chicks by vaccination at the first day of life. Further cross-protection trials should be done, including other Av. paragallinarum strains in the vaccine, especially those from serogroup A. 相似文献
78.
Lais S. B. Dias Dayane de S. Silva Gleidson Giordano P. de Carvalho Maria Leonor G. M. L. de Araújo Fabiano F. da Silva Mara Lúcia A. Pereira Jefferson R. Gandra Victor G. O. Lima Antnio C. S. dos Santos Lucas F. de A. Bulco Vagner M. Leite Jos Esler de Freitas Júnior 《Animal Science Journal》2020,91(1)
This study aimed to investigate the effects of chitosan and whole raw soybean on nutrient intake, apparent digestibility, nitrogen utilization, microbial protein synthesis, blood metabolites, feeding behavior, ruminal fermentation, digesta kinetics, and reticular flow of nutrients of buffaloes. Four ruminally‐cannulated Murrah buffaloes (351 ± 15 kg of initial BW) were randomly assigned according to a 4 × 4 Latin square design. Treatments were arranged as 2 × 2 factorial arrangement: the first factor was whole raw soybean (WRS), and the second factor was chitosan (CHI) with or without their inclusion in diets. Intake and apparent digestibility of ether extract (p < .01; p = .04, respectively), non‐fiber carbohydrates intake (p = .03) and apparent ruminal digestibility of dry matter (p = .01) were affected by diets. An interaction effect or tendency was observed for microbial nitrogen (p = .09), concentrations, ruminal ammonia nitrogen (p = .05), total volatile fatty acid (p = .03). Association of chitosan with whole raw soybean has potential effects as a modulator of rumen fermentation; therefore, chitosan can be applied as an alternative non‐ionophore for Murrah buffaloes. 相似文献
79.
Histological alterations in gills of shrimp Litopenaeus vannamei in low‐salinity waters under different stocking densities: Potential relationship with nitrogen compounds 下载免费PDF全文
Marcela G. Fregoso‐López María S. Morales‐Covarrubias Miguel A Franco‐Nava Javier Ramírez‐Rochín Juan F Fierro‐Sañudo Jesús T Ponce‐Palafox Federico Páez‐Osuna 《Aquaculture Research》2017,48(12):5854-5863
Two experimental modules with different stocking densities (M1 = 70 and M2 = 120 shrimp /m2) were examined weekly over a culture cycle in tanks with low‐salinity water (1.9 g/L) and zero water exchange. Results showed survival rates of 87.7 and 11.9% in M1 and M2, respectively. Water temperature, pH, dissolved oxygen, electrical conductivity and chlorophyll a were not significantly (p > .05) different between modules. In contrast, the concentrations of nitrogen compounds were significantly (p < .05) different between modules, except nitrite‐N (M2 were 2.31 ± 1.38 mg/L N‐TAN, 0.18 ± 0.49 mg/L N‐NO2? and 6.83 ± 6.52 mg/L N‐NO3?; in M1: 0.97 ± 0.73 mg/L N‐TAN, 0.05 ± 0.21 mg/L N‐NO2? and 0.63 ± 0.70 mg/L N‐NO3?). When waters of both modules reached higher levels of ammonia and nitrite, histological alterations were observed in gills. The histological alterations index (HAI) was higher in M2 (5‐112) than in M1 (2‐22). 相似文献
80.
Mapping of genes involved in glutathione, carbohydrate and COR14b cold induced protein accumulation during cold hardening in wheat 总被引:6,自引:0,他引:6
G. Galiba I. Kerepesi A. Vágújfalvi G. Kocsy L. Cattivelli J. Dubcovsky J.W. Snape J. Sutka 《Euphytica》2001,119(1-2):173-177
Using some of the chromosome substitution lines developed from thecrosses of the donor Cheyenne to Chinese Spring we showed that theaccumulation of water soluble carbohydrates during different stages ofhardening was time dependent. Moreover there was a significantcorrelation between the rate of carbohydrate accumulation and the frosttolerance. The expression and regulation of a wheat gene homologous tothe barley cold regulated cor14b gene was compared in frost sensitiveand frost tolerant wheat genotypes at different temperatures. Studies madewith chromosome substitution lines showed that the threshold inductiontemperature polymorphism of the cor14b wheat homologous genewas controlled by loci located on chromosome 5A of wheat, while cor14b gene was mapped, in Triticum monococcum, onto the longarm of chromosome 2Am. Our study on the effect of cold hardeningon glutathione (GSH) metabolism showed that chromosome 5A of wheathas an influence on the GSH accumulation and on the ratio of reduced andoxidised glutathione as part of a complex regulatory function during coldhardening. In addition, the level of increase in GSH content duringhardening may indicate the degree of the frost tolerance of wheat. 相似文献