Proximal hindlimb flexion in the horse: Effect on movement symmetry and implications for defining soundness

Reasons for performing the study: Flexion tests are a common tool during the prepurchase and clinical lameness examination, yet studies quantifying the effect of flexion, apart from distal forelimb flexion in sound horses, are sparse. Objectives: To investigate the effect of proximal hindlimb flexion on perceivable and measurable changes in movement symmetry in horses with objective movement symmetry falling within the margins of ‘sound’. Methods: Thirteen horses, selected based on objective movement symmetry, were instrumented with inertial sensors on left and right tuber coxae and over the os sacrum. Vertical movement symmetry was quantified at trot before and after proximal hindlimb flexion, with a repeat of flexion after 5 min. Video recordings of the horses were assessed visually. Results: Proximal hindlimb flexion introduced additive changes in movement symmetry to the individual baseline movement, with a tendency towards smaller effects with increasing stride number. The main systematic effect was a decrease in upward movement of the os sacrum following mid‐stance of the flexed limb and an increase in upward movement following mid‐stance of the nonflexed limb, also manifesting in a ‘hip hike’ of the flexed limb; these findings reflected increased movement asymmetry following flexion. Depending on individual baseline asymmetry, flexion can also increase movement symmetry. Conclusions: Proximal hindlimb flexion can exacerbate subtle asymmetry when performed carefully. Variation in measured symmetry following flexion within and between horses showed that the individual response to flexion is highly variable. Potential relevance: Proximal hindlimb flexion may elevate the asymmetry of a slightly lame limb above the threshold for visibility, thus assisting in the clinical gait examination. Further work is needed to examine the causes for a positive response to flexion and possible differences between sound and lame horses as well as horses of different athletic disciplines.     

Vegetation-type effects on performance and meat quality of growing Engadine and Valaisian Black Nose sheep grazing alpine pastures

Lambs of two Swiss mountain breeds, Engadine Sheep (ES; n=55) and Valaisian Black Nose Sheep (VS; n=55), were fattened in 2010 and 2011 on one lowland and three alpine pastures. Groups of six to seven lambs per breed were allocated to pastures of a size allowing ad libitum intake on the following four vegetation types: (i) species-poor and nutrient-rich lowland (400 m a.s.l.) ryegrass–clover ley on flat terrain (‘lowland-rich’); (ii) alpine, species-rich and nutrient-rich vegetation type on a flat terrain (1950 m; ‘alpine-rich’); (iii) alpine, species-rich vegetation type, moderate in nutrients on steep terrain (2150 m; ‘alpine-moderate’); and (iv) alpine, species-rich and nutrient-poor vegetation type on steep terrain (2150 m; ‘alpine-poor’). Lambs were slaughtered after 9 weeks of grazing in a commercial slaughterhouse. Carcasses were subjected to the Swiss CH-TAX classification grid. Meat quality was analysed on the Musculus longissimus dorsi (LD) in the segment of Musculus longissimus lumborum. Forage quality varied among vegetation types as anticipated. Across all sites, ES were superior (P<0.001) to VS in dressing percentage (43.9 vs. 38.4), carcass weight (18.4 vs. 16.4 kg), meat conformation and fat cover scores, even though daily gains had been lower (105 vs. 122 g/d; P<0.05). Meat of ES contained more dry matter, protein, total ash (P<0.001) and fat (P<0.05). Their meat was darker and more intensive red, had lower cooking losses (23.0 vs. 25.1%) but was less tender (65.6 vs. 58.1 N) compared to VS (P<0.001). Vegetation types offering good quality forage (lowland-rich and alpine-rich) enhanced average daily gains, dressing percentage, meat conformation and fat cover scores (mostly P<0.05 against alpine-moderate and -poor). Similarly, a higher vegetation quality promoted a higher intramuscular fat and lower cooking losses. Meat from lowland-rich was darkest and had the lowest shear force (P<0.05). Almost no breed×site interactions occurred in growth performance as well as carcass and meat quality. It was therefore concluded that both breeds are similarly well adapted to the extensive pasture production system in alpine regions. Still, ES seem to be the more efficient one of the two sheep breeds. The results also indicate that growth performance and meat quality clearly vary between vegetation types in animals grazing alpine pastures.     

Variation in resistance to the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in Pietrain and Miniature pigs

Porcine reproductive and respiratory syndrome (PRRS) is one of the economically most important diseases of swine. Viraemia and the prolonged persistence of the virus are among the most critical factors. Virus replication and severity of disease vary with virus isolates, and there is rising evidence for a genetic component of the host susceptibility. Dissecting the genetic basis of resistance/susceptibility to PRRS virus (PRRSV) might lead to improved knowledge on the molecular mechanisms of PRRS and the establishment of genetic markers for future disease control. The aim of this study was to establish a porcine model with emphasized genetic differences in PRRSV susceptibility. Seven ‘Wiesenauer Miniature’ pigs (MI), a local German breed and eight commercial Pietrain (PI) pigs were challenged with 10⁵ TCID₅₀ of an attenuated PRRSV strain (Ingelvac^® PRRSV MLV). Clinical status, viraemia and seroconversion of the pigs were compared. No clinical signs were observed during the experiment. Viraemia peaked on day 6 p.i., with 100% of viraemic pigs in PI and on day 12 p.i with 87% of viraemic MI. Viraemia lasted for up to 35 days in MI and for at least 72 days in PI. This surprising result was confirmed by a second study with another four MI. MI and PI showed maximum virus titres of 10^2.5 TCID₅₀/ml of serum and 10^4.5 TCID₅₀/ml, respectively, indicating a virus replication in MI of approximately 3.3% that of PI over the complete period. MI were more efficient in antibody production. With such pronounced breed differences, the model is of high relevance for the genetic dissection of PRRS pathogenesis and susceptibility.     

Genetic diversity of rhizobia associated with alfalfa in Serbian soils

We have evaluated the genetic diversity and phylogeny of alfalfa rhizobia, originating from different types of soils in Serbia and their ability to establish an effective symbiosis with alfalfa (Medicago sativa L.). A collection of 65 strains isolated from root nodules of alfalfa were characterized by rep-PCR analysis, partial and complete 16S rDNA gene and recA gene sequencing, as well as atpD gene sequencing and DNA–DNA hybridizations. The results of the sequence analyses revealed that Sinorhizobium meliloti is the dominant species in alfalfa nodules. Only one strain was identified as Sinorhizobium medicae, two strains as Rhizobium tibeticum and one strain as Rhizobium sp. Despite the fact that the majority of strains were identified as S. meliloti, a high genetic diversity at strain level was detected. Almost all isolates shared the ability to nodulate and fix nitrogen with M. sativa, except 11 of them, which were incapable of fixing nitrogen with this species. About 50% of the isolates showed values of symbiotic effectiveness (SE) above 50%, while 10% of the strains were highly effective with SE values above 70%. Some of the strains which were highly effective in nitrogen fixation at the same time could intensively solubilize phosphates, offering a possibility for multipurpose inoculum development. This was the first genetic study of rhizobia isolated from this region and also the first report of natural presence of R. tibeticum in root nodules of M. sativa.     

Analysis of bacterial load and prevalence of mixed infections with Lawsonia intracellularis, Brachyspira hyodysenteriae and/or Brachyspira pilosicoli in German pigs with diarrhoea

Lawsonia (L.) intracellularis, Brachyspira (B.) hyodysenteriae and B. pilosicoli are important pathogens in domestic pig production world-wide, responsible for porcine intestinal adenomatosis, swine dysentery, and porcine intestinal spirochetosis, respectively. Conventional PCR is the major diagnostic tool in the detection of the three pathogens, but the sole detection of bacterial DNA might lead to misinterpretations of results with respect to their clinical relevance, especially with mixed infections. Thus, the present study targeted the detection and quantification of the three pathogens in samples from herds with a case history of diarrhoea. Herds and samples were selected by the practitioners on a voluntary basis. Results were based on 1176 individual samples from 95 herds from Southern Germany. The pathogens were detected simultaneously by multiplex real-time PCR. The overall prevalence for L. intracellularis, B. hyodysenteriae and B. pilosicoli was 12.6%, 8.4% and 3.2% in faecal samples and 48.4%, 24.2% and 31.6% in herds, respectively. Sixty one percent, 82.6%, and 73.4% of herds positive for L. intracellularis, B. hyodysenteriae, and B. pilosicoli, respectively, had mixed infections. Median log values of DNA equivalents/g of faeces for L. intracellularis, B. hyodysenteriae and B. pilosicoli were 3.3, 5.9 and 3.2, with maxima of 8.3, 8.0 and 6.3, respectively. Within herd prevalence of B. hyodysenteriae and B. pilosicoli as well as the load of B. hyodysenteriae were significantly associated with the severity of diarrhoea.     

In vitro culture of equine respiratory mucosa explants

An in vitro model of the upper respiratory tract of the horse was developed to investigate mechanisms of respiratory diseases. Four tissues of the upper respiratory tract of three horses were collected. Explants were maintained in culture at an air–liquid interface for 96 h. At 0, 24, 48, 72 and 96 h of cultivation, a morphometric analysis was performed using light microscopy, scanning electron microscopy and transmission electron microscopy. The explants were judged on morphometric changes of epithelium, basement membrane and connective tissue. Viability was evaluated using a fluorescent Terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labelling (TUNEL) staining. No significant changes in morphometry and viability of any of the explants were observed during cultivation. Hence, the in vitro model may be useful to study infectious and non-infectious diseases at the level of the equine respiratory tract, with potential application to the development of vaccines and treatments for diseases of the respiratory tract.     

Vapour intrusion from the vadose zone—seven algorithms compared

Background, aim and scope  

Vapours of volatile organic compounds (VOCs) emanating from contaminated soils may move through the unsaturated zone to the subsurface. VOC in the subsurface can be transported to the indoor air by convective air movement through openings in the foundation and basement. Once they have entered the building, they may cause adverse human health effects. Screening-level algorithms have been developed, which predict indoor air concentrations as a result of soil (vadose zone) contamination. The present study evaluates seven currently used screening-level algorithms, predicting vapour intrusion into buildings as a result of vadose zone contamination, regarding the accuracy of their predictions and their usefulness for screening purpose. Screening aims at identifying contaminated soils that should be further investigated as to the need of remediation and/or the presence of an intolerable human health risk. To be useful in this respect, screening-level algorithms should be sufficiently conservative so that they produce very few false-negative predictions but they should not be overly conservative because they might have insufficient discriminatory power.