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981.
Paul C Reunamo A Lindehoff E Bergkvist J Mausz MA Larsson H Richter H Wängberg SÅ Leskinen P Båmstedt U Pohnert G 《Marine drugs》2012,10(4):775-792
Several marine and freshwater diatoms produce polyunsaturated aldehydes (PUA) in wound-activated processes. These metabolites are also released by intact diatom cells during algal blooms. Due to their activity in laboratory experiments, PUA are considered as potential mediators of diatom-bacteria interactions. Here, we tested the hypothesis that PUA mediate such processes in a close-to-field mesocosm experiment. Natural plankton communities enriched with Skeletonema marinoi strains that differ in their PUA production, a plankton control, and a plankton control supplemented with PUA at natural and elevated concentrations were observed. We monitored bacterial and viral abundance as well as bacterial community composition and did not observe any influence of PUA on these parameters even at elevated concentrations. We rather detected an alternation of the bacterial diversity over time and differences between the two S. marinoi strains, indicating unique dynamic bacterial communities in these algal blooms. These results suggest that factors other than PUA are of significance for interactions between diatoms and bacteria. 相似文献
982.
Pistocchi R Guerrini F Pezzolesi L Riccardi M Vanucci S Ciminiello P Dell'Aversano C Forino M Fattorusso E Tartaglione L Milandri A Pompei M Cangini M Pigozzi S Riccardi E 《Marine drugs》2012,10(1):140-162
The Northern Adriatic Sea is the area of the Mediterranean Sea where eutrophication and episodes related to harmful algae have occurred most frequently since the 1970s. In this area, which is highly exploited for mollusk farming, the first occurrence of human intoxication due to shellfish consumption occurred in 1989, nearly 10 years later than other countries in Europe and worldwide that had faced similar problems. Until 1997, Adriatic mollusks had been found to be contaminated mostly by diarrhetic shellfish poisoning toxins (i.e., okadaic acid and dinophysistoxins) that, along with paralytic shellfish poisoning toxins (i.e., saxitoxins), constitute the most common marine biotoxins. Only once, in 1994, a toxic outbreak was related to the occurrence of paralytic shellfish poisoning toxins in the Adriatic coastal waters. Moreover, in the past 15 years, the Adriatic Sea has been characterized by the presence of toxic or potentially toxic algae, not highly widespread outside Europe, such as species producing yessotoxins (i.e., Protoceratium reticulatum, Gonyaulax spinifera and Lingulodinium polyedrum), recurrent blooms of the potentially ichthyotoxic species Fibrocapsa japonica and, recently, by blooms of palytoxin-like producing species of the Ostreopsis genus. This review is aimed at integrating monitoring data on toxin spectra and levels in mussels farmed along the coast of the Emilia-Romagna region with laboratory studies performed on the species involved in the production of those toxins; toxicity studies on toxic or potentially toxic species that have recently appeared in this area are also reviewed. Overall, reviewed data are related to: (i) the yessotoxins producing species P. reticulatum, G. spinifera and L. polyedrum, highlighting genetic and toxic characteristics; (ii) Adriatic strains of Alexandrium minutum, Alexandrium ostenfeldii and Prorocentrum lima whose toxic profiles are compared with those of strains of different geographic origins; (iii) F. japonica and Ostreopsis cf. ovata toxicity. Moreover, new data concerning domoic acid production by a Pseudo-nitzschia multistriata strain, toxicity investigations on a Prorocentrum cf. levis, and on presumably ichthyotoxic species, Heterosigma akashiwo and Chattonella cf. subsalsa, are also reported. 相似文献
983.
984.
Paul G. Wahome Kevin R. Beauchesne Anna C. Pedone John Cavanagh Christian Melander Paul Zimba Peter D. R. Moeller 《Marine drugs》2015,13(1):65-75
Aquatic microbes produce diverse secondary metabolites with interesting biological activities. Cytotoxic metabolites have the potential to become lead compounds or drugs for cancer treatment. Many cytotoxic compounds, however, show undesirable toxicity at higher concentrations. Such undesirable activity may be reduced or eliminated by using lower doses of the cytotoxic compound in combination with another compound that modulates its activity. Here, we have examined the cytotoxicity of four microbial metabolites [ethyl N-(2-phenethyl) carbamate (NP-1), Euglenophycin, Anabaenopeptin, and Glycolipid 652] using three in vitro cell lines [human breast cancer cells (MCF-7), mouse neuroblastoma cells (N2a), and rat pituitary epithelial cells (GH4C1)]. The compounds showed variable cytotoxicity, with Euglenophycin displaying specificity for N2a cells. We have also examined the modulatory power of NP-1 on the cytotoxicity of the other three compounds and found that at a permissible concentration (125 µg/mL), NP-1 sensitized N2a and MCF-7 cells to Euglenophycin and Glycolipid 652 induced cytotoxicity. 相似文献
985.
Alessandra Andrisani Gabriella Donà Elena Tibaldi Anna Maria Brunati Chiara Sabbadin Decio Armanini Gualtiero Alvisi Salvatore Gizzo Guido Ambrosini Eugenio Ragazzi Luciana Bordin 《Marine drugs》2015,13(9):5533-5551
Astaxanthin (Asta), a photo-protective red pigment of the carotenoid family, is known for its multiple beneficial properties. In this study, the effects of Asta on isolated human sperm were evaluated. Capacitation involves a series of transformations to let sperm acquire the correct features for potential oocyte fertilization, including the generation of a controlled amount of reactive oxygen species (ROS), cholesterol depletion of the sperm outer membrane, and protein tyrosine phosphorylation (Tyr-P) process in the head region. Volunteers, with normal spermiogram values, were divided in two separate groups on the basis of their ability to generate the correct content of endogenous ROS. Both patient group (PG) and control group (CG) were analysed for Tyr-phosphorylation (Tyr-P) pattern and percentages of acrosome-reacted cells (ARC) and non-viable cells (NVC), in the presence or absence of Asta. In addition, the involvement of ROS on membrane reorganization and the presence of Lyn, a Src family kinase associated with lipid rafts, were investigated. Results show that Lyn is present in the membranes of human sperm, mainly confined in midpiece in resting conditions. Following capacitation, Lyn translocated to the head concomitantly with raft relocation, thus allowing the Tyr-P of head proteins. Asta succeeded to trigger Lyn translocation in PG sperm thus bypassing the impaired ROS-related mechanism for rafts and Lyn translocation. In this study, we showed an interdependence between ROS generation and lipid rafts and Lyn relocation leading the cells to undergo the successive acrosome reaction (AR). Asta, by ameliorating PG sperm functioning, may be utilised to decrease male idiopathic infertility. 相似文献
986.
Anna Lettieri Rosaria Esposito Adrianna Ianora Antonietta Spagnuolo 《Marine drugs》2015,13(3):1451-1465
The anti-proliferative effects of diatoms, described for the first time in copepods, have also been demonstrated in benthic invertebrates such as polychaetes, sea urchins and tunicates. In these organisms PUAs (polyunsaturated aldehydes) induce the disruption of gametogenesis, gamete functionality, fertilization, embryonic mitosis, and larval fitness and competence. These inhibitory effects are due to the PUAs, produced by diatoms in response to physical damage as occurs during copepod grazing. The cell targets of these compounds remain largely unknown. Here we identify some of the genes targeted by the diatom PUA 2-trans-4-trans-decadienal (DD) using the tunicate Ciona intestinalis. The tools, techniques and genomic resources available for Ciona, as well as the suitability of Ciona embryos for medium-to high-throughput strategies, are key to their employment as model organisms in different fields, including the investigation of toxic agents that could interfere with developmental processes. We demonstrate that DD can induce developmental aberrations in Ciona larvae in a dose-dependent manner. Moreover, through a preliminary analysis, DD is shown to affect the expression level of genes involved in stress response and developmental processes. 相似文献
987.
Sara Hareardóttir Marina Pan?i? Anna Tammilehto Bernd Krock Eva Friis M?ller Torkel Gissel Nielsen Nina Lundholm 《Marine drugs》2015,13(6):3809-3835
Diatoms of the genus Pseudo-nitzschia produce domoic acid (DA), a toxin that is vectored in the marine food web, thus causing serious problems for marine organisms and humans. In spite of this, knowledge of interactions between grazing zooplankton and diatoms is restricted. In this study, we examined the interactions between Calanus copepodites and toxin producing Pseudo-nitzschia. The copepodites were fed with different concentrations of toxic P. seriata and a strain of P. obtusa that previously was tested to be non-toxic. The ingestion rates did not differ among the diets (P. seriata, P. obtusa, a mixture of both species), and they accumulated 6%–16% of ingested DA (up to 420 µg per dry weight copepodite). When P. seriata was exposed to the copepodites, either through physical contact with the grazers or separated by a membrane, the toxicity of P. seriata increased (up to 3300%) suggesting the response to be chemically mediated. The induced response was also triggered when copepodites grazed on another diatom, supporting the hypothesis that the cues originate from the copepodite. Neither pH nor nutrient concentrations explained the induced DA production. Unexpectedly, P. obtusa also produced DA when exposed to grazing copepodites, thus representing the second reported toxic polar diatom. 相似文献
988.
Larval population structure of Engraulis encrasicolus in the Strait of Sicily as revealed by morphometric and genetic analysis
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Angela Cuttitta Bernardo Patti Teresa Maggio Enza Maria Quinci Anna Maria Pappalardo Venera Ferrito Vito De Pinto Marco Torri Francesca Falco Aldo Nicosia Marianna Musco Grazia Maria Armeri Francesco Placenti Giorgio Tranchida Roberta Mifsud Angelo Bonanno Salvatore Mazzola 《Fisheries Oceanography》2015,24(2):135-149
In order to investigate the larval population structure, specimens of Engraulis encrasicolus larvae from five different locations in the Strait of Sicily were analyzed by means of otolith readings, morphometric and genetic techniques. The distribution of age in day, identified by means of the otolith readings and associated with the oceanographic parameters, was useful to identify possible spawning areas and transport dynamics. The presence of more than one spawning area suggested the possibility that two or more sub‐populations may co‐exist in the study area. The morphometric characteristics were more adequate than genetic parameters to discriminate the different larval groups. The most relevant variables for the separation were the mouth length (ML) and the body diameter (BD). The population structure by means of genetic data reported the presence of two phylogroups co‐occurring among samples in each sampling locations specimens. The pattern of genetic divergence among anchovy larvae in the Strait of Sicily was congruent with previous studies conducted on adult populations present in other Mediterranean areas with different molecular markers. The habitat‐specific nature of the morphological variation and the lack of corresponding genetic variation among larvae from the different locations suggested that the observed differences in morphology could be linked to environmental parameters. The body form differences among different larvae samples could reflect the nutritional status of larvae. In fact, these differences were found among anchovy larvae collected in areas with different oxygen and fluorescence, which is an index of primary productivity and is linked to the availability of food for anchovy larvae. 相似文献
989.
Comparative growth and mortality of cultured Lion's Paw scallops (Nodipecten subnodosus) from Gulf of California and Pacific populations and their reciprocal transplants
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![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Volker Koch Anna Rengstorf Marc Taylor José M. Mazón‐Suástegui Francisco Sinsel Matthias Wolff 《Aquaculture Research》2015,46(1):185-201
This study focused on the comparison of growth and mortality of Lion's Paw scallops Nodipecten subnodosus from Gulf of California and Pacific populations and their reciprocal transplants, cultured in suspended module and bottom culture in Baja California Sur, Mexico. Environmental parameters were monitored to determine site‐specific differences and were correlated with scallops' population‐specific responses. Significant differences in growth were found between sites, populations and grow‐out methods. Growth performance of scallops originating from the Pacific population was lower and uniform between Pacific and Gulf culture sites, whereas Gulf scallops cultured at the Pacific site grew better. Growth and survival was higher in suspended than bottom culture, mainly due to depth‐associated differences in food and oxygen supply. Increasing temperatures and decreasing food and oxygen during summer months resulted in depressed growth and elevated mortality. At the Pacific site, this was partially compensated by considerably higher food availability. Gulf scallops resisted high diurnal temperature changes at San Buto significantly better than Pacific scallops. They also survived generally better than the Pacific population, independent of site and grow‐out method, indicating physiological adaptations to high temperatures and low food availability in their habitat. Consequently, the Gulf population appears to be more feasible for aquaculture operations in coastal lagoons with stressful conditions. 相似文献