全文获取类型
收费全文 | 2502篇 |
免费 | 138篇 |
国内免费 | 4篇 |
专业分类
林业 | 172篇 |
农学 | 107篇 |
基础科学 | 4篇 |
578篇 | |
综合类 | 172篇 |
农作物 | 224篇 |
水产渔业 | 150篇 |
畜牧兽医 | 1037篇 |
园艺 | 58篇 |
植物保护 | 142篇 |
出版年
2024年 | 3篇 |
2023年 | 32篇 |
2022年 | 63篇 |
2021年 | 106篇 |
2020年 | 97篇 |
2019年 | 108篇 |
2018年 | 93篇 |
2017年 | 88篇 |
2016年 | 94篇 |
2015年 | 88篇 |
2014年 | 103篇 |
2013年 | 162篇 |
2012年 | 182篇 |
2011年 | 200篇 |
2010年 | 114篇 |
2009年 | 81篇 |
2008年 | 173篇 |
2007年 | 150篇 |
2006年 | 131篇 |
2005年 | 120篇 |
2004年 | 100篇 |
2003年 | 84篇 |
2002年 | 70篇 |
2001年 | 34篇 |
2000年 | 22篇 |
1999年 | 18篇 |
1998年 | 6篇 |
1997年 | 11篇 |
1996年 | 11篇 |
1995年 | 5篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 11篇 |
1991年 | 5篇 |
1990年 | 11篇 |
1989年 | 3篇 |
1987年 | 2篇 |
1986年 | 5篇 |
1985年 | 9篇 |
1984年 | 6篇 |
1983年 | 6篇 |
1982年 | 2篇 |
1980年 | 3篇 |
1977年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1958年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有2644条查询结果,搜索用时 0 毫秒
111.
Claire Bouvard Isabelle Galy-Fauroux Fran?oise Grelac Wassila Carpentier Anna Lokajczyk Sophie Gandrille Sylvia Colliec-Jouault Anne-Marie Fischer Dominique Helley 《Marine drugs》2015,13(12):7446-7462
Low-molecular-weight fucoidan (LMWF) is a sulfated polysaccharide extracted from brown seaweed that presents antithrombotic and pro-angiogenic properties. However, its mechanism of action is not well-characterized. Here, we studied the effects of LMWF on cell signaling and whole genome expression in human umbilical vein endothelial cells and endothelial colony forming cells. We observed that LMWF and vascular endothelial growth factor had synergistic effects on cell signaling, and more interestingly that LMWF by itself, in the absence of other growth factors, was able to trigger the activation of the PI3K/AKT pathway, which plays a crucial role in angiogenesis and vasculogenesis. We also observed that the effects of LMWF on cell migration were PI3K/AKT-dependent and that LMWF modulated the expression of genes involved at different levels of the neovessel formation process, such as cell migration and cytoskeleton organization, cell mobilization and homing. This provides a better understanding of LMWF’s mechanism of action and confirms that it could be an interesting therapeutic approach for vascular repair. 相似文献
112.
Yang DK Shin EK Oh YI Kang HK Lee KW Cho SD Song JY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1077-1082
We analyzed the nucleotide sequences of the G-L (glycoprotein-large protein) intergenic non-coding region of 33 strains of the rabies virus (RABV) isolated in South Korea in 1998-2010 and compared the sequences with those of previously reported non-Korean strains. The similarities of the nucleotide sequences of the G-L region among all Korean RABV isolates ranged from 97.1 to 100%. Based on the phylogenetic analysis of the G-L region, the Korean RABV isolates were classified into three distinct subgroups with high similarity and were most closely related to the non-Korean NeiMeng1025C isolate, which was isolated from a rabid raccoon dog in eastern China, suggesting that the Korean RABV isolates originate from a rabid raccoon dog in northeastern Asia. Our results indicated that G-L region, as a useful phylogenetic indicator, is equivalent to the nucleoprotein (N) or glycoprotein (G) gene for study of RABV molecular epidemiology and that the Korean RABV isolates showing a few substitutions in the G-L region are continuously circulating in South Korea. 相似文献
113.
Shin JH Sakoda Y Kim JH Ochiai K Umemura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1167-1169
Rabbits were immunized with inactivated influenza virus via the subarachnoidal (SA) or subcutaneous (SC) route, and the antibody titers in cerebrospinal fluid (CSF) and serum were assayed. There were no nervous signs or morphological lesions related to SA immunization. In the SC group, the antibody titer was elevated in serum, but not elevated in CSF. In the SA group, the antibody titer was significantly elevated in serum and even in CSF, and their antibody titers were much greater than in the SC group. The present results suggest that intrathecal immunization is more effective than SC immunization at inducing a protective immune response against the transneural spread of viruses. 相似文献
114.
Yea Hwang MOON Ji Un OK Shin Ja LEE Jong Kyu HA Sung Sill LEE 《Animal Science Journal》2010,81(6):642-647
A comparative study among Korean native cow (Hanwoo), Holstein dairy cow, Korean native goat and crossbred sheep on the population and marker concentration of ruminal microbes, the activities of carboxymethylcellulase (CMCase), xylanase and amylase, and in situ dry matter (DM) degradability were conducted. Twelve ruminally cannulated animals, three of each species, were used. Animals were fed the same diet containing 40% formula feed and 60% rice straw at the level of 2.5% of body weight. Total viable microbial populations in the rumen fluid were significantly (P < 0.01) greater for bacteria and fungi in goat than those of Holstein. The protozoan population among ruminant species was the reverse from that of bacteria. The concentrations of 2,6‐diaminopimelic acid and chitin as markers for bacteria and fungi in the rumen fluid, respectively, were highest in goat, which is in accordance with the above population data. The concentration of aminoethylphosphonic acid as marker of protozoa was highest in Hanwoo and lowest in sheep (P < 0.01). Goat had the highest (P < 0.01) activities of all the enzymes investigated among ruminants. In situ effective degradation of the DM of rice straw was approximately 19% higher in the rumen of goat compared with other animals. 相似文献
115.
Wies?aw Szulczewski Ma?gorzata Biniak-Pieróg Anna Machowczyk 《Agricultural Water Management》2010,97(5):587-595
The paper presents a weather-yield model developed for the purpose of estimating spring barley yield on the basis of dry spells occurring in individual periods between the phenological phases of that plant. For that purpose research material on spring barley, originating from the years 1976-1997, was used as well as diurnal sums of precipitation. Five periods were considered in the analysis: sowing-emergence, emergence-tillering, tillering-heading, heading-milk ripeness and milk ripeness-full ripeness. In the study a model of changes in the amount of water available for plant during rainless periods was used. Five measures were adopted for characterisation of the approximation error: correlation coefficient, mean relative error, relative root mean square error, model efficiency and coefficient of residual mass. The analyses performed demonstrated that yield reduction is significantly influenced by rainless periods that occur in the sowing-emergence and tillering-heading inter-phase periods. The adopted criteria for yield reduction estimation show considerable similarity for the emergence-tillering and heading-milk ripeness inter-phase periods. At the same time, their influence on yield reduction is three-fold lower than during the sowing-emergence and tillering-heading inter-phase periods. Analyses performed with the use of the developed model indicate that yield size is affected by rainless periods of duration longer than 30% of the inter-phase period. 相似文献
116.
Rescifina A Chiacchio U Iannazzo D Piperno A Romeo G 《Journal of agricultural and food chemistry》2010,58(22):11876-11882
Complexes of β-cyclodextrin (β-CD) and caffeine (Caf) with biophenols present in olive and olive oil (tyrosol, hydroxytyrosol, homovanillic acid, 3,4-dihydroxyphenylacetic acid, and protocatechuic acid) were investigated by NMR spectroscopy and thermodynamical-molecular dynamic studies to verify the formation of supermolecular aggregates. The obtained results indicated that the investigated biophenols form inclusion complexes with β-CD in a molar ratio of 1:1 in aqueous solution having binding constant values from 10- to 40-fold bigger than those of the corresponding complexes with Caf. Then, β-CD preferentially encloses the biophenol molecule, decreasing its bitter taste and, at the same time, preserving it against chemical and physical decomposition reactions that occur during storage. 相似文献
117.
Predictors of pregnancy and postpartum haemoglobin concentrations in low-income women 总被引:1,自引:0,他引:1
OBJECTIVE: Pregnancy and postpartum iron status is of great public health importance, yet few studies have examined predictors of haemoglobin (Hb) concentration during this time. We identified predictors of Hb from 24 weeks' gestation until delivery and from 4 to 25 weeks postpartum. DESIGN: Blood was drawn as many as four times during care: at the initial visit, at 24-29 weeks' gestation, at delivery and postpartum. A longitudinal, multivariable linear regression model was used to predict Hb concentration. SETTING: A public health clinic in Raleigh, North Carolina.Subjects: n=520 women who participated in the Iron Supplementation Study. RESULTS: Hb concentration at the previous blood draw, short stature, non-Hispanic white ethnicity/race, >12 years of education and smoking were positive predictors of pregnancy and postpartum Hb concentrations. Iron supplement use was a positive predictor, while inadequate weight gain and severe nausea/vomiting were negative predictors of gestational Hb. A high infant birth weight and postpartum haemorrhage were negative predictors of postpartum Hb. Pre-pregnancy body mass index had a slight positive relationship with gestational Hb, but had a strong negative relationship with postpartum Hb. The longitudinal model also confirmed the typical pattern of gestational Hb concentration. As the number of weeks between the initial visit and the 24- to 29-week visit increased, Hb at 24-29 weeks' gestation decreased. As gestational age increased from 24 weeks until delivery, Hb concentration increased as well. CONCLUSIONS: The predictors identified here could be used in clinical settings to target high-risk women for intervention. 相似文献
118.
Ji Hyeon Ahn Joon Ha Park In Hye Kim Jae-Chul Lee Bing Chun Yan Min Sik Yong Choong Hyun Lee Jung Hoon Choi Ki-Yeon Yoo In Koo Hwang Seung Myung Moon Hyung-Cheul Shin Moo-Ho Won 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):335-342
Melatonin affects diverse physiological functions through its receptor and plays an important role in the central nervous system. In the present study, we compared immunoreactivity patterns of arylalkylamine N-acetyltransferase (AANAT), an enzyme essential for melatonin synthesis, and melatonin receptor type 1B (MT2) in the spinal cord of young adult (2~3 years) and aged (10~12 years) beagle dogs using immunohistochemistry and Western blotting. AANAT-specific immunoreactivity was observed in the nuclei of spinal neurons, and was significantly increased in aged dog spinal neurons compared to young adult spinal neurons. MT2-specific immunoreactivity was found in the cytoplasm of spinal neurons, and was predominantly increased in the margin of the neuron cytoplasm in aged spinal cord compared to that in the young adult dogs. These increased levels of AANAT and MT2 immunoreactivity in aged spinal cord might be a feature of normal aging and associated with a feedback mechanism that compensates for decreased production of melatonin during aging. 相似文献
119.
为了解不同磷水平下雷竹Phyllostachys violascens幼苗叶片叶绿素荧光特性的变化,试验以无性繁殖的雷竹幼苗作为试验材料,采用砂培试验方法,研究了不同磷水平(0,0.5,5.0,50.0,500.0 mg·L-1)对雷竹幼苗叶绿素荧光参数和光通量密度?鄄光合电子传递速率(PAR-ETR)响应曲线的影响。结果表明,随着磷质量浓度的升高,幼苗叶片潜在光化学活性(Fo /Fm)呈逐渐降低趋势,最大光化学效率(Fv /Fm)在0~50.0 mg·L-1磷质量浓度范围内逐渐下降。磷质量浓度为5.0 mg·L-1时,初始荧光(Fo),最大荧光(Fm),适时最小荧光(Ft)和可变荧光(Fv)均达到最大值,电子传递速率(ETR)和光系统Ⅱ(PSⅡ)实际光化学效率(Y)较大,光化学猝灭系数(qP)和非光化学猝灭系数(qN和NPQ)相对较低,说明在5.0 mg·L-1磷质量浓度下,雷竹幼苗叶片的光合能力较强,接近适宜雷竹幼苗生长的磷质量浓度。磷质量浓度过高(50.0和500.0 mg·L-1)均导致雷竹幼苗叶片ETR下降,热耗散增加,光化学效率和光量子产额降低。图1表3参20 相似文献
120.
Anna Chlopecka 《Water, air, and soil pollution》1993,69(1-2):127-134
Barley (Hordeum vulgare L.) was grown on a sandy soil given different doses of cadmium carbonate (salt), copper carbonate (malachite), lead carbonate (cerussite), and zinc carbonate (smithsonite) in a pot experiment conducted in a greenhouse. The element compounds were added to the soil in amounts equivalent to the following levels of the metals: Cd 5, 10, 50 μq ?1; Cu and Pb 50, 100, 500 μg g?1; Zn 150, 300, 1500 μg g?1. Sequential extraction was used for partition these metals into five operationally-defined fractions: exchangeable, bound to carbonates, bound to Fe-Mn oxides, bound to organic matter and residual. The residue was the most abundant fraction in the untreated soil for all the metals studied (43 to 61% of the total contents). The concentration of exchangeable Cd (0.2 μg g?1), Cu (0.01 μg g?1), Pb (0.1 μg g?1), and Zn (1.4 μg g?1) were relatively low in the untreated soil but increased markedly in the treated soils for Cd (up to 31 μg g?1) and Zn (up to 83 μg g?1), whereas only small changes were observed for Cu and Pb. The pot experiment showed a significant increase in the Cd and Zn contents of barley grown on the treated soils, but only small changes in Cu and Pb concentrations. 相似文献