Perinuclear antineutrophil cytoplasmic autoantibodies in dogs infected with various vector-borne pathogens and in dogs with immune-mediated hemolytic anemia

Objective-To determine the prevalence of perinuclear antineutrophil cytoplasmic autoantibodies (pANCA) in dogs with confirmed or suspected immune-mediated hemolytic anemia (IMHA) or dogs infected with various vector-borne pathogens, including Rickettsia rickettsii, Bartonella henselae, Bartonella vinsonii subsp berkhoffii, Ehrlichia canis, Borrelia burgdorferi, and Leishmania infantum. Animals-55 dogs with confirmed or suspected IMHA, 140 dogs seroreactive for vector-borne pathogens, and 62 healthy dogs and dogs seronegative for vector-borne pathogens. Procedures-Samples were allocated to subgroups on the basis of the health status of the dogs and the degree of seroreactivity against various vector-borne pathogens. Serum samples were tested retrospectively via indirect immunofluorescence assay to determine pANCA status. Results-26 of 55 (47%) dogs with confirmed or suspected IMHA and 67 of 140 (48%) dogs seroreactive for vector-borne pathogens had positive results when tested for pANCA. Serum samples with the highest antibody concentrations against L infantum antigen had the highest proportion (28/43 [65%]) that were positive for pANCA. One of 20 (5%) dogs seronegative for tick-borne pathogens and 8 of 22 (36%) dogs seronegative for L infantum had positive results for pANCA. One of 20 (5%) healthy dogs had serum antibodies against pANCA. Conclusions and Clinical Relevance-pANCA were detected in a high percentage of dogs with IMHA and vector-borne infectious diseases. Therefore, pANCA may be a relatively nonspecific marker for dogs with inflammatory bowel disease, although they could represent a biomarker for immune-mediated diseases and infections.     

Retained Fetal Membranes in Heavy Draft Mares Associated with Histological Abnormalities

Retention of fetal membranes is one of the most common life-threatening peripartum problems; however, its etiology is still unknown. Draft mares are more likely to retain fetal membranes than other breeds of mares. Because a strong connection between the fetal membranes and the endometrium has been observed in cases of retention, we studied the changes in the endometrium and the allantochorion of heavy draft mares with this condition. Tissue samples were taken from 90 mares that retained the fetal membranes and from 21 that did not. Retained fetal membranes were associated with adhesion of the allantochorion to the endometrium in 88% of the mares; this adhesion depended on the following factors: occurrence of fibrosis both in the lamina propria of the allantochorial villi and in the allantochorial stromal connective tissue, overdevelopment of the allantochorial stromal connective tissue, oversized allantochorial epithelial cells, and sparser and less-branched allantochorial villi.     

Redistribution of Runoff Among Vegetation Patch Types: On Ecohydrological Optimality of Herbaceous Capture of Run-On

A central tenant of ecohydrology in drylands is that runoff redistribution from bare to vegetated patches concentrates the key limiting resource of water, which can then enhance vegetation growth and biomass. Conversely, a reduction in vegetation patches, particularly those associated with herbaceous plants, can lead to a threshold-like response in which bare patches become highly interconnected, triggering a large increase in hillslope runoff and associated erosion. However, generally lacking is an assessment of how maximization of run-on to herbaceous patches relates to minimization of hillslope-scale runoff. To illustrate how runoff redistribution potentially changes in response to conversion of herbaceous patches to bare ones, we used a spatially distributed model, SPLASH (Simulator for Processes at the Landscape Surface–Subsurface Hydrology), with an example of a semiarid piñon–juniper woodland hillslope with seven combinations of bare and herbaceous patch cover, culminating in complete loss of herbaceous patches, for a 1-yr design storm. As expected, the amount of hillslope runoff increased curvilinearly with reductions in herbaceous cover as runoff per cell increased from bare patches and run-on per cell increased for herbaceous patches. Notably, the total amount of run-on to all herbaceous patches was greatest when the amount of bare cover was intermediate, highlighting a trade-off between the source area for generating runoff and the sink area for capturing run-on. The specific nature of patch–hillslope runoff redistribution responses certainly depends on several site-specific conditions, but the general nature of the response exhibited in our example simulation may be indicative of a general type of response applicable to many rangelands. We suggest that a more robust suite of such relationships could be valuable for managing rangelands by enabling explicit accounting for optimality and trade-offs in biomass per herbaceous patch, total herbaceous cover, and prevention of hillslope-scale connectivity of bare patches that triggers a large increase in runoff and associated erosion.     

Gender differences in exercise - induced intravascular haemolysis during race training in thoroughbred horses

Exercise-induced intravascular haemolysis and “sport anemia” are widely reported in human sports medicine. It has been recognized also in horses, however, the clinical importance and the onset of this condition seem different than in human. In this study we investigated the episodes of intravascular haemolysis, indicated by the increase in plasma haemoglobin and the decrease in serum haptoglobin levels, after routine training sessions in race horses. Heart rate and changes in haematological parameters confirmed, that the exertion was relatively high. Intravascular haemolysis did not appear in stallions but was detected in mares after two training sessions. It has been determined that serum haptoglobin levels were higher in mares than in stallions before and after all training sessions. It is postulated that intravascular haemolysis induced by training is of limited clinical importance because it occurred only in mares which are better adapted due to higher haptoglobin level at rest, and it had no cumulative effect. Therefore gender differences should be taken into consideration in experiments with athletic horses.     

Re-emergence of rabies in Mazowieckie Voivodeship,Poland, 2021

Due to the oral vaccination of foxes against rabies most of the territory of Poland was freed from rabies of non-flying mammals. In January 2021, rabies was diagnosed in fox in the central part of Mazowieckie Voivodeship where rabies has not been detected since last 17 years. Subsequently, in the following months the rabies virus infection spread southward reaching the voivodeship of Świętokrzyskie in November 2021. Emergency actions were implemented aiming at rapid rabies elimination.     

An application of temperature mapping of horse's back for leisure horse‐rider‐matching

Leisure riding is a popular way of using horses however, unlike sport or racing horses, those are mostly not associated with one rider with high skills. Constant overload of equine musculoskeletal system causes pathologies, which are affecting horse mobility and decreases the horse‐rider communication. The aim was to propose the new scoring system of thermograph analysis as an aspect of differences in heat distributions on horseback before and after leisure ridings. The study was conducted on sixteen Polish warmblood horses, scanned with a non‐contact thermographic camera. Heat pattern of the thoracolumbar area was evaluated on thermograms taken before and after exercise. The criteria with point values for horse‐rider‐matching were created: heat points on the dorsal midline of saddle‐back contact area and degree of muscle unit overload. The results of thermograph analysis were compared with the results of a questionnaire on horse‐rider communication during riding in order to estimate the relevance of matching. The maximum score was obtained in 38.3% and 39.8% of combinations based on the thermograph analysis and questionnaire, respectively. Results of both scoring systems were strongly positive correlated (r = .937), demonstrating high sensitivity (61.72%) and specificity (90.23%) of the matching. The horse‐rider matching may improve horse comfort during leisure type of work.     

Comparison of milk protein composition in a Swedish and a Danish dairy herd using reversed phase HPLC

Abstract

The milk composition in a Swedish herd, consisting of Swedish Red and White cows (SRB) and Swedish Holstein cows (SLB), and in a Danish herd, consisting of Danish Holstein-Friesian cows (SDM), was evaluated. Concentrations of the major milk proteins (κ-casein, α_S1-casein, β-casein, β-lactoglobulin A, β-lactoglobulin B and α-lactalbumin) of 134 individual milk samples were determined by reversed phase (RP) HPLC. Other parameters determined included milk fat, urea, lactose, calcium, lactoferrin, somatic cell count and protein degradation (determined as level of free amino-terminals). Analysis of variance was used to compare concentrations of analysed milk variables between SRB and SLB or between SLB and SDM. Concentration of total protein, total casein, β-casein and κ-casein were significantly higher in SRB milk compared with SLB milk. Concentration of α-lactalbumin and calcium were significantly higher in SDM milk than in SLB milk. The concentration of urea was higher in SLB than in SDM milk and is suggested to reflect differences in feeding regimes between the investigated Swedish and Danish herd.