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排序方式: 共有242条查询结果,搜索用时 31 毫秒
31.
Hartmann AD Wilhelm N Balzarini J Hartmann K 《Journal of Feline Medicine and Surgery》2012,14(2):107-112
In in vitro studies, the acyclic nucleoside phosphonate 9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine (PMPDAP) inhibited the replication of feline immunodeficiency virus (FIV). No information about its clinical efficacy is available so far. The aim of this prospective placebo-controlled, double-blinded study was to evaluate the antiviral efficacy of PMPDAP in cats naturally infected with FIV. Twenty cats were randomly assigned to two treatment groups receiving either PMPDAP (25 mg/kg) or placebo twice per week subcutaneously for 6 weeks. The general health status (Karnofsky's score), clinical signs, laboratory, immunological, and surrogate parameters were evaluated. No significant differences were found between PMPDAP- and placebo-treated cats, although cats treated with PMPDAP showed a tendency for improvement in their Karnofsky's score and clinical signs. Haematological side effects were noted in the PMPDAP-treated cats. Thus, PMPDAP may be an option in treating cats if it becomes available for veterinarians, but side effects have been monitored. 相似文献
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Jores J Nkando I Sterner-Kock A Haider W Poole J Unger H Muriuki C Wesonga H Taracha EL 《Veterinary immunology and immunopathology》2008,124(1-2):192-197
Contagious bovine pleuropneumonia (CBPP) is a lung disease caused by the bacterial pathogen Mycoplasma mycoides ssp. mycoides small colony type (MmmSC). It has been spreading due to a number of factors including poor vaccine efficacy and poor sensitivity of current diagnostic tests. The purpose of this study was to assess interferon gamma (IFN-gamma) release after stimulation of peripheral blood mononuclear cells (PBMC) from experimentally infected cattle. PBMC collected from 15 artificially infected animals were incubated with different concentrations of total MmmSC antigen. After 72h of incubation the IFN-gamma release was measured and found to be elevated in 11 animals. We did not observe a correlation between IFN-gamma release of animals with and without pathomorphological gross lesions. Therefore, our data do not confirm a role for CD4 T-lymphocytes in protection, since there is no correlation between IFN-g secretion (supposed to be mainly derived from CD4 T-cells) and disease severity. Additionally, we applied immunocytochemistry on affected lung tissue and detected no build up of T-lymphocytes (CD4 T-cells, CD8 T-cells) but a high presence of myeloid cells. 相似文献
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In order to evaluate the seroconversion of horses to Babesia caballi and B. canis in Hungary, blood samples were collected from 371 animals on 23 different locations of the country. The presence of antibodies to B. caballi was screened with a competitive ELISA. All 29 positive samples came from one region (the Hortobágy). The prevalence of infection did not show correlation with sexes, and reached 100% in the age group of 2-5 years. Babesia canis-specific antibodies were demonstrated by IFAT in 6.74% of animals kept in 7 regions. The titres were low or medium level (1:40 to 1:160), indicating that the horses had previously been exposed to this piroplasm, but their infection must have been limited. The highest seropositivity rate was observed in the age group of 3-4 years, and males (stallions and geldings) were significantly more frequently infected than females. However, neither B. caballi nor B. canis could be identified in the peripheral blood samples of infected horses by PCR. Since most of the B. caballi-positive horses remained negative in the B. canis IFAT, whereas seroconversion solely to B. canis was detected in several regions of the country, serological cross-reaction between the two species can be discounted. This is the first serological evidence of horses being naturally infected with B. canis, supporting the view that piroplasms are less host specific than previously thought. 相似文献
38.
Molecular tracing confirms that infection with infectious pancreatic necrosis virus follows the smolt from hatchery to grow‐out farm
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Anja B. Kristoffersen Magnus Devold Vidar Aspehaug Ove Gjelstenli Olav Breck Britt Bang Jensen 《Journal of fish diseases》2018,41(10):1601-1607
Infectious pancreatic necrosis (IPN) is an important restraint to production of salmonids in aquaculture globally. In order to implement efficacious mitigation strategies for control of this disease, it is important to understand infection routes under current production systems. IPN virus has been shown to be transmitted vertically in Rainbow trout, from broodstock to fingerlings in hatcheries, and there is circumstantial evidence suggesting that vertical transmission can also occur in Atlantic salmon, in addition to horizontal transmission between grow‐out fish in farms. In this study, we show that the smolt carries infection with IPN from hatchery to the marine farm. We do this by comparing sequences from fish groups taken both in hatcheries and on corresponding marine grow‐out farms. We use statistical analysis to prove that sequences obtained from the same fish group in both hatchery and marine farm are more similar than sequences obtained from random fish groups on hatcheries and marine farms. 相似文献
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Lotus E. Kam Francisco J. Martínez‐Cordero Pingsun Leung Anthony C. Ostrowski 《Aquaculture Economics & Management (Blackwell Science)》2013,17(1-2):95-123
Abstract Economic evaluations of commercial milkfish (Chanos chanos) growout systems were made based on current Hawaiian fishpond, pond and tank culture techniques in Hawaii. Based on the observed practices of milkfish culture as a secondary or tertiary crop, capital costs and several operating costs were pro‐rated to accurately depict current farm practices. For a target harvest weight of 0.80 lb, the study estimates the total production cost for milkfish production at $3.31/lb for the pond system, $3.81/lb for the tank system and $1.84/lb for the Hawaiian fishpond system. At a sale price of $3.00/lb and seed cost of $0.25/fingerling, only the Hawaiian fishpond system is profitable under a 20‐year project life. When considering variable costs alone, the production cost is $1.78/lb for the pond system, $2.33/lb for the tank system and $1.53 for the Hawaiian fishpond system, yielding returns on variable costs of 67%, 30% and 59% respectively. Analyses of profit sensitivity to sale price, production yield, labor, feed and stocking indicate that sale price, as expected, has the largest impact on profitability, followed by feed. The results of this study are consistent with the Hawaii farmers’ view of milkfish as a secondary specie to core production based upon current market conditions and input requirements. 相似文献
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Tuija Pehkonen Janne Koskimki Kaisu Riihinen Anna Maria Pirttil Anja Hohtola Laura Jaakola Anne Tolvanen 《Physiological and Molecular Plant Pathology》2008,72(4-6):146-150
An inoculation method for Exobasidium splendidum and Exobasidium vaccinii was developed on the dwarf shrub Vaccinium vitis-idaea. Using inoculated ramets, we investigated whether there are differences between V. vitis-idaea populations in the susceptibility to Exobasidium infections and whether the defence reaction of V. vitis-idaea is visible at a molecular level. Sixteen V. vitis-idaea clones from four populations were propagated in tissue cultures and the ramets were inoculated with E. splendidum or E. vaccinii fungi. The expression of three flavonoid biosynthetic genes (chalcone synthase, dihydroflavonol 4-reductase and anthocyanidin synthase) and the accumulation of flavonoids and hydroxycinnamic acids were determined in response to E. splendidum infection. A pathogenesis-related (PR 4) gene was isolated and its expression was studied in host ramet leaves. To our knowledge, this was the first successful artificial infection reported with E. splendidum. Disease frequencies of the inoculated ramets were between 32% and 47% for E. splendidum and 33% for E. vaccinii, but below 10% in uninoculated control ramets. There were no differences in disease frequencies between V. vitis-idaea populations. Both symptomatic leaves and healthy leaves of diseased ramets showed activation of flavonoid biosynthesis at the gene level, whereas expression of PR 4 was observed only in symptomatic leaves. The increase of flavonoid biosynthesis in healthy leaves of diseased ramets may represent a general response to stress or a role in defence against the pathogen E. splendidium. Ability of V. vitis-idaea to defend chemically against Exobasidium fungi and the heterogeneity of genotypes, age, size, and growth rates in host plant populations might be reasons for the low infection incidence of Exobasidia in nature. 相似文献