Influences of day and night temperatures on flowering of Fragaria x ananassa Duch., cvs. Korona and Elsanta,at different photoperiods

The effects of photoperiod (12, 13, 14, 15 or 16 h), day temperature (12, 15, 18, 24 or 27 °C) and night temperature (6, 9 or 12 °C) and their interactions on flower and inflorescence emergence were investigated by exposing 4 week old runner plants of strawberry cvs. Korona and Elsanta during a period of 3 weeks. A daily photoperiod of 12 or 13 h resulted in the highest number of plants with emerged flowers. A photoperiod of 14 h or more strongly reduced this number, while no flowers emerged at a photoperiod of 16 h. Plants exposed to photoperiods of 12 or 13 h flowered earlier and had longer flower trusses. A day temperature of 18 °C and/or a night temperature of 12 °C were optimal for plants to emerge flowers and resulted in the shortest time to flowering. A night temperature of 6 °C strongly reduced the number of plants that emerged flowers, especially when combined with lower day temperatures. Photoperiod and temperature had no effect on the number of inflorescences, all flowering plants produced on average one inflorescence. The number of flowers on the inflorescence increased with decreasing day temperature and when photoperiod was raised from 12 to 15 h. In general, ‘Korona’ was more sensitive to photoperiod and temperature as ‘Elsanta’, and had a lower optimal day temperature for flower emergence. Results of this experiment may be used to produce high quality plant material or to define optimal conditions when combining flower induction and fruit production.     

Latitudinal variability in the seroprevalence of antibodies against Toxoplasma gondii in non-migrant and Arctic migratory geese

Toxoplasma gondii is an intracellular coccidian parasite found worldwide and is known to infect virtually all warm-blooded animals. It requires a cat (family Felidae) to complete its full life cycle. Despite the absence of wild felids on the Arctic archipelago of Svalbard, T. gondii has been found in resident predators such as the arctic fox and polar bear. It has therefore been suggested that T. gondii may enter this ecosystem via migratory birds. The objective of this study was to identify locations where goose populations may become infected with T. gondii, and to investigate the dynamics of T. gondii specific antibodies. Single blood samples of both adults and juveniles were collected from selected goose species (Anser anser, A. brachyrhynchus, Branta canadensis, B. leucopsis) at Arctic brood-rearing areas in Russia and on Svalbard, and temperate wintering grounds in the Netherlands and Denmark (migratory populations) as well as temperate brood-rearing grounds (the Netherlands, non-migratory populations). A modified agglutination test was used on serum, for detection of antibodies against T. gondii. Occasional repeated annual sampling of individual adults was performed to determine the antibody dynamics. Adults were found seropositive at all locations (Arctic and temperate, brood-rearing and wintering grounds) with low seroprevalence in brood-rearing birds on temperate grounds. As no juvenile geese were found seropositive at any brood-rearing location, but nine month old geese were found seropositive during spring migration we conclude that geese, irrespective of species and migration, encounter T. gondii infection in wintering areas. In re-sampled birds on Svalbard significant seroreversion was observed, with 42% of seropositive adults showing no detectable antibodies after 12 months, while the proportion of seroconversion was only 3%. Modelled variation of seroprevalence with field data on antibody longevity and parasite transmission suggests seroprevalence of a population within a range of 5.2–19.9%, in line with measured values. The high occurrence of seroreversion compared to the low occurrence of seroconversion hampers analysis of species- or site-specific patterns, but explains the absence of an increase in seroprevalence with age and the observed variation in antibody titre. These findings imply that even though infection rate is low, adults introduce T. gondii to the high Arctic ecosystem following infection in temperate regions.     

Bovine herpesvirus 5 BICP0 complements the bovine herpesvirus 1 homolog

Bovine herpesvirus 1 (BoHV-1) and BoHV-5 are closely related (82% amino acid identity) but differ strongly in neuropathogenesis. The immediate-early gene for BICP0 is less conserved (70% amino acid identity) and may contribute to a dissimilar phenotype. A peculiar difference is a guanosine hexamer in the BICP0-1 gene which aligns with only five guanosines in the BICP0-5 gene and therefore results in a frameshift in the latter open reading frame. Thus, the C-terminal amino acid sequence (residues 643–676 of BICP0-1 vs. 655–720 of BICP0-5) is completely different. We introduced the BICP0-5 frameshift into the BoHV-1 genome cloned as a bacterial artificial chromosome (BoHV-1 BAC) using the Red recombination system with galK selection and counterselection. Transfection of MDBK cells with the resulting BAC produced recombinant virus that replicated like wild type BoHV-1 in vitro. Attempts to exchange the entire BICP0-1 gene by the BoHV-5 homolog using the same approach failed repeatedly. Therefore, we cotransfected purified BICP0⁻/galK⁺-BoHV-1 BAC DNA with a recombination plasmid coding for BICP0-5 with or without a HA tag into MDBK cells. BoHV-1 recombinants expressing the respective proteins were characterized. In vitro, all recombinants grew to similar titers as the parental viruses, which demonstrates that BICP0-5 compensates for the growth defect of BICP0⁻/galK⁺-BoHV-1 and functionally complements BICP0-1 of BoHV-1. We conclude that BICP0 may be suitable to positively select BoHV-1 recombinants with deletions or insertions of additional genes of interest.     

Cornification defect in the golden retriever: clinical, histopathological, ultrastructural and genetic characterisation

Veterinarians have recognised a nonpruritic skin disease in the Golden retriever breed characterised by excessive scaling of large, variably pigmented flakes of skin in otherwise healthy dogs. This prospective case series describes clinical, histopathological, ultrastructural and genetic features of this cornification defect in 17 affected dogs. The condition affects young dogs of either sex and is characterised by symmetrical, predominantly ventro-lateral scaling and hyperpigmentation of the trunk. Histopathological and ultrastructural changes of the stratum corneum are suggestive of delayed degradation of corneodesmosomes. A genetic aetiology is proposed and a single-trait autosomal recessive mode of inheritance discussed.     

Mechanical stress grading of tropical timbers without regard to species

Some reports have shown that for single species the correlation between modulus of elasticity (MOE) and modulus of rupture (MOR) in bending is quite high. Tropical timbers consist of hundreds of species that are difficult to identify. This report deals with the mechanical stress grading of tropical timber regardless of species. Nine timber species or groups of species with a total number of 1094 pieces measuring 60 × 120 × 3000 mm, were tested in static bending. The MOE was measured flat wise, while MOR was tested edge wise. Statistical analysis of linear regression with a dummy model and analysis of covariance were used to analyze the role of MOE and the effect of species on prediction of MOR. The analysis showed that using MOE as a single predictor caused under/overestimation for one or more species and/or groups of species. The accuracy of prediction would be increased with species identification. An allowable stress and reference resistance for species and/or groups of species were provided to compare with the prediction of strength through timber grading. The timber strength class for species and/or groups of species was also established to support the application of mechanical timber grading.