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31.
Kuznedelov K Minakhin L Niedziela-Majka A Dove SL Rogulja D Nickels BE Hochschild A Heyduk T Severinov K 《Science (New York, N.Y.)》2002,295(5556):855-857
In bacteria, promoter recognition depends on the RNA polymerase sigma subunit, which combines with the catalytically proficient RNA polymerase core to form the holoenzyme. The major class of bacterial promoters is defined by two conserved elements (the -10 and -35 elements, which are 10 and 35 nucleotides upstream of the initiation point, respectively) that are contacted by sigma in the holoenzyme. We show that recognition of promoters of this class depends on the "flexible flap" domain of the RNA polymerase beta subunit. The flap interacts with conserved region 4 of sigma and triggers a conformational change that moves region 4 into the correct position for interaction with the -35 element. Because the flexible flap is evolutionarily conserved, this domain may facilitate promoter recognition by specificity factors in eukaryotes as well. 相似文献
32.
Bennett AD McKnight GE Dodkin SJ Simpson KE Schwartz AM Gunn-Moore DA 《Journal of Feline Medicine and Surgery》2011,13(2):152-154
Measuring urine specific gravity (USG) is an important component of urine analysis as it evaluates renal concentrating capability. The objective of this study was to quantify the difference in USG values between a hand-held optical analogue refractometer and a cat-specific digital instrument. Urine samples from 55 cats were assessed. There was a statistically significant difference between these two refractometers (P<0.001), with the optical refractometer (mean USG=1.031) consistently reading higher than the digital refractometer (mean USG=1.027). Results for a random subset of the samples (n=10) were compared with urine osmolality and both the optical and digital instruments demonstrated excellent correlation. While an accurate USG reading is important, it is unlikely that the statistical significance between the two instruments is clinically significant and, therefore, unlikely to result in a change in patient evaluation or treatment plans. While both the digital and optimal refractometers are highly correlated to the urine osmolality, making both devices valid for assessment of USG in clinical practice, this digital device is easier to read and eliminates the variability of subjective interpretation. 相似文献
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34.
Magni C Herndl A Sironi E Scarafoni A Ballabio C Restani P Bernardini R Novembre E Vierucci A Duranti M 《Journal of agricultural and food chemistry》2005,53(11):4567-4571
The prevalence of food allergies in the world population requires integrated approaches to identify new potential allergens, especially those of plant origin. The aim of this work was the allergen in vitro analysis of Lupinus albus seed proteome, a promising food protein source, and the assessment of IgE cross-reactivities with other more diffused legume species. A combination of one- and two-dimensional gel electrophoresis and immunoblotting analyses with specific IgGs for band identification and lupin-sensitized patients' circulating IgEs for allergenicity studies has been used. Two lupin proteins, namely, conglutin gamma and 11S globulin basic subunits, strongly reacted with all patients' sera. Also, cross-reactivities with the homologous polypeptides of other legume species were observed. Otherwise, no reaction at all was detected with a 2S-type lupin protein. This global electrophoretic approach has allowed the identification of a new potential lupin allergen and confirmed the cross-reactivity among the legume 11S globulin basic subunits. 相似文献
35.
36.
Jürgen Weber Jupp Kreutzmann Anita Plantikow Evelyn Claus Werner Manz Peter Heininger 《Journal of Soils and Sediments》2006,6(2):84-91
Goal, Scope and Background Numerous xenobiotics released into surface waters are transferred to suspended particulate matter and finally attached to
sediments. Aquatic organisms may be exposed to them by direct particle feeding, by physical contact with contaminated surfaces
as an exposure route, and by the uptake of dissolved contaminants after equilibration via the free water phase. In order to
assess potential sediment toxicity, each of these exposure routes has to be addressed. This paper presents a newly developed
particle contact assay that uses the fermentation performance of a specific Saccharomyces cerevisiae strain for the assessment
of toxic effects in sediments. The test procedure is based on the characteristic feature of growing yeast cells to attach
to sediment particles, which are also relevant for the accumulation of contaminants. The physical contact with lipophilic
contaminants mirrors an exposition pathway for the direct uptake into the cells. In order to quantitatively characterize the
toxic effects of particle attached pollutants on the fermentation performance, unpolluted native reference sediment was spiked
with representatives for widely distributed anthropogenic contaminants.
Methods Saccharomyces cerevisiae was established as sensitive eukaryotic microorganism for the ecotoxicological assessment of particle
attached anthropogenic contaminants in freshwater sediments. For this purpose, yeast cells were cultivated in sediment samples
and the resulting fermentation performance was continuously measured. Sediments artifically spiked with HCB, PCB, g-HCH, DDT,
and benzo(a)pyrene and solutions of each contaminant were comparatively investigated by means of their adverse effects on
yeast fermentation performance. Additionally, four native river sediments characterized by increasing levels of pollution
were assessed by the yeast particle contact assay, and simultaneously by standard aquatic tests with algae, daphniae, and
luminescent bacteria using pore water and elutriates. Results of the bioassays were related to specific sediment contamination
with respect to metals and organic priority pollutants.
Results and Discussion In sediments spiked with PCB and benzo(a)pyrene fermentation, performance was affected extensively below concentrations inhibiting
fermentation in contaminant solutions. This suggests a high efficiency of the exposure route by physical contact. The fermentation
performance was only slightly affected by single lipophilic pollutants, whereas mixtures of individually spiked sediments
caused critically reduced fermentation performance suggesting additive synergistic effects. Native river sediments modestly
to critically polluted by hazardous organic compounds lead to a slightly to dangerously reduced fermentation performance in
the yeast contact assay. These inhibitory effects were much less pronounced in the standard bioassays conducted with algae,
daphniae and luminescent bacteria, applying pore waters and elutriates as sample matrices. Using pore water, inhibition was
measured only in the most polluted sediment, elutriates lead to a slight inhibition of the algal growth in the undiluted sample
only. These results indicate an improved sensitivity of the yeast particle contact assay compared to the standard assays,
due to uptake and physical cell contact as additional routes of exposure.
Conclusion The yeast particle contact assay is a valuable tool for the assessment of ecotoxicological potential in freshwater sediments.
Since the assay addresses physical contact as an exposure route, it indicates bioavailability of lipophilic compounds in sediments.
Outlook The sensitive indication of bioavailable contaminants associated to sediment particles by the newly developed yeast particle
contact assay recommends it as a complementary microbial bioassay in a test battery for assessing major pathways of contaminants
in whole sediments. 相似文献
37.
38.
The natural division of the pig's liver into lobules was established at this stage without particular attention to the pattern of blood vessels—in order to explain missing relationships in the structure of the liver parenchyma. The results of the study, which diviate from what has been reported to date, enable us to give a first overview and make comparisons with histological sections. 相似文献
39.
Susan M. Newell DVM Mary B. Mahaffey DVM MS Royce E. Roberts DVM MS Ana Patricia Garcia DVM Anita R. Stampley DVM 《Veterinary radiology & ultrasound》1994,35(3):217-220
Laryngeal adenocarcinoma was diagnosed in a 5-year-old golden retriever dog with no history of respiratory or pharyngeal difficulties. Radiographically the basihyoid bone was destroyed by the neoplasm, and extensive soft tissue mineralization ventral to the larynx was also present. Complete surgical resection was not possible due to diffuse involvement of the tongue and larynx. Cobalt-60 teletherapy was used for treatment of the tumor. There was no clinical evidence of tumor regrowth at approximately 12 months post treatment. This is an unusual example of primary laryngeal neoplasia due to the absence of clinical respiratory abnormalities and the aggressive destruction of the basihyoid bone. 相似文献
40.
配合饲料、浓缩饲料或予混添加剂中的维生素 A 经过碱性皂化作用、乙醚提取和蒸干后,溶于甲醇中,然后在 HPLC 上利用萤光检测仪在 Ex=325nm;Em=480nm 条件下进行了测定。测定所用条件为:固定相:Spherisorb ODSⅡ;流动相:甲醇:水=90:10;流速1.00ml/min。维生素 A 保留时间5.3分钟。测定结果表明,维生素 A 的回收率在94%以上,不同饲料样本变异系数(CV%)范围在4.1-7.5之间。 相似文献