Landscape-ecological mapping of the Netherlands

The Landscape-ecological Mapping of the Netherlands project (LMN project) started in 1983 with the aim of establishing a landscape-ecological database for use in developing and evaluating national land-use plans. The project, working with grid cells of 1 km², has four working objectives: a) development of mapping potential for basic landscape-ecological data, b) assessment of susceptibility to interventions, c) evaluation of significance for nature conservation and d) production of vulnerability maps, as a combination of susceptibility and significance. In addition to information on soil, groundwater, ecotopes, flora and fauna, the database also incorporates information on physiographical features and entire landscapes. The resulting database is a geographic information system (GIS). This article describes the second phase of the project (1985–1989), covering the Randstad area, and focusses on the methods and the applications potential of the database.     

Wastage and disease in Bay of Plenty dairy herds

Information on disease incidence and herd wastage was collected from 36 farms supplying the Rangitaiki Plains Dairy Company over a two-year period from June 1981 to May 1983. These herds contained 7050 milking cows and 1460 yearlings. Although 33.5% of calves horn were reared, only 21.7% entered the herd. The average wastage rate over the two seasons was 22.6%. This included a 2.5% death-rate. Main reasons for culling were infertility (6.3%), low production (3.7%) and mastitis (2.3%). Only 0.8% of cows died or were culled because of bloat and 0.4% from each of facial eczema and metabolic diseases. Treatments were administered by farmers or veterinarians to 18.8% of the cows for mastitis, to 3.9% for metabolic diseases and to 3.6% for lameness. In total, 34.6% of cows received treatment for disease. Wide differences were noted in the proportion of cases of different diseases for which veterinarians were consulted. These ranged from 83.6% for scours to 3.8% for mastitis. As well as confirming or quantifying the relative importance of various diseases and causes of wastage, the survey highlighted the problems in collecting reliable data from herd owners on the events in their herds.     

Roaring in Clydesdales

An endoscopic survey was performed to assess arytenoid cartilage movement during quiet respiration in 48 Clydesdale horses. Physical characteristics which could influence the length of the recurrent laryngeal nerves were also recorded from these horses and the relationship of these characteristics to the presence of abnormal left arytenoid cartilage movements was assessed statistically. Some degree of abnormal arytenoid movement was observed in 50% of the animals over one year of age. No significant correlation between sex, age, estimated weight, height, or neck length could be found although affected animals were, on average, slightly taller, lighter and longer necked.     

A model to study the effect of certification of planting material on the occurrence of leek rust

Based on a simple model, the possible effects of certification of planting material on the occurrence of leek rust in a region can be derived from information on the proportion of infected fields (v) and the proportion of newly planted fields with infected planting material (i) in that region. Ifv i, certification of planting material will be highly effective.     

Cell-mediated immune responses in cattle adult-vaccinated with Brucella abortus strain 19 and in cattle infected with Brucella abortus field strain.

Cell-mediated immune responses in cattle adult-vaccinated with Brucella abortus strain 19, cattle infected with B abortus field strain, and nonexposed cattle were studied by an in vitro lumphocyte-stimulation test (LST). Lymphocytes were prepared from peripheral bovine blood by the Ficoll-diatrizoate technique, and results were assayed for [3H]thymidine incorporation into DNA by liquid scintillation spectrometry. Serotests and bacteriologic isolation attempts were conducted simultaneously with LST. Lymphocytes from cattle infected with field strains had significantly (P = 0.01) higher specific lymphocyte-stimulation inexposed controls. The LST, the serum standard-tube agglutination test (STT), the Rivanol (RIV) test, and the complement-fixation (CF) test correctly classified cattle from which field strains and strain 19 of B abortus were isolated. The LST was negative in cattle vaccinated with B abortus strain 19 (nonshedding), but the three serotests had many false-positive reactions. The CF test had the least false-positive reaction, followed by the RIV test, and the STT was the least specific. Well before the three serotests became positive, the LST was positive in samples from some cattle during the incubation period of the infection. There was little or no correlation between cell-mediated immune responses (as measured by LST) and serum antibody responses (as measured by STT, RIV test, and CF test) in vaccinated but culture-negative cattle and in some nonvaccinated cattle during the incubation period.     

Longitudinal studies of naturally acquired Brucella abortus infection in sheep.

Naturally acquired Brucella abortus infections were studied during consecutive pregnancies in eight sheep and in their lambs over a period of 40 months to evaluate epizootiologic aspects of natural infection in sheep. Brucella abortus was isolated from the ewes following 16 of 26 natural terminations of pregnancy: from 5 of 6 ewes in the first year, from six of eight ewes in the second year, from two of six ewes in the third year, and from three of six ewes in the fourth year. Vaginal swab samples and milk samples were the most consistent source of the brucella organisms. Brucella abortus was isolated from three ewes when standard tube test seroagglutination titers were less than 1:100. In contrast, results of supplemental tests (card, 2-mercaptoethanol, complement-fixation, and Rivanol) remained positive during the study. During the 40 months, B abortus was isolated from 4 of 4 aborted fetuses, 2 of 5 stillborn lambs, 10 of 37 living lambs, and as an indicator of continuing infection, from 6 of 12 lambs born during the fourth year. Although B abortus has a definite host preference for cattle, this study demonstrated that under appropriate management conditions, sheep may be naturally infected and may remain infected for more than 40 months. Epizootiologic evaluation of all factors, including husbandry practices and exposure potential, should be utilized in determining the need to test other species that may have been exposed to cattle infected with B abortus.