Synthesis of (-)-[4-3H]epigallocatechin gallate and its metabolic fate in rats after intravenous administration

Because a great deal of attention has been focused on the metabolism of (-)-epigallocatechin gallate (EGCg), quantitative analysis of this compound is required. For this purpose we developed a method of chemical synthesis of [4-(3)H]EGCg. Synthesized [4-(3)H]EGCg showed 99.5% radiochemical purity and a specific activity of 13 Ci/mmol. To clarify the excretion route of EGCg, the radioactivity levels of bile and urine were quantified after intravenous administration of [4-(3)H]EGCg to bile-duct-cannulated rats. Results showed that the radioactivity of the bile sample excreted within 48 h accounted for 77.0% of the dose, whereas only 2.0% of the dose was recovered in the urine. The excretion ratio of bile to urine was calculated to be about 97:3. These results clearly showed that bile was the major excretion route of EGCg. Time-course analysis of the radioactivity in blood was also performed to estimate the pharmacokinetic parameters following intravenous administration of [4-(3)H]EGCg. In addition, EGCg metabolites excreted in the bile within 4 h after the intravenous dose of [4-(3)H]EGCg were analyzed by HPLC. The results showed that 4',4"-di-O-methyl-EGCg was present in the conjugated form and made up about 14.7% of the administered radioactivity.     

Detection of Brucella melitensis in semen using the polymerase chain reaction assay

An evaluation of the polymerase chain reaction (PCR) for detection of Brucella melitensis DNA in bovine and ovine semen was performed. Since semen contains different components that inhibit PCR amplification, a protocol was used to purify Brucella-DNA from bovine and ovine semen samples prior to conducting amplification of the targeted DNA. When separated fractions of naturally Brucella contaminated semen were analyzed by the PCR, most of B. melitensis DNA were present in the seminal fluid and non-sperm fractions.The PCR examination results for detection of B. melitensis DNA in different semen fractions were compared with the results for traditional cultural methods of Brucella from semen. The PCR was more sensitive than the traditional cultural methods since it detected Brucella-DNA in 12 (10%) out of 120 semen samples while direct culture detected only 7 (5.8%) in the same semen samples. The limit of detection by PCR was 100 CFU/ml of semen. In addition, the results of PCR were available in one day, whereas isolation and identification of Brucella organisms required days or even weeks. The PCR may be used as a supplementary test for detection of B. melitensis in semen.     

Comparison of carprofen and butorphanol as post-surgical analgesics in cats

Post‐operative pain management by a single subcutaneous (SC) injection of carprofen has been found to be effective in cats and dogs. This clinical study compared the analgesic properties of injectable carprofen and butorphanol in 71 healthy cats (0.5–5 years, mean weight 3.24 ± 0.61 kg) undergoing ovariohysterectomy. Cats were randomly assigned to three groups: Group C received carprofen 4 mg kg^?1 SC at intubation and sterile saline 0.08 mL kg^?1 SC at extubation; Group B received sterile saline 0.08 mL kg^?1 SC at intubation and butorphanol 0.4 mg kg^?1 SC at extubation; Group S received sterile saline 0.08 mL kg^?1 SC at intubation and extubation. All cats were pre‐medicated with atropine (0.04 mg kg^?1 SC), acepromazine (0.02 mg kg^?1 SC), ketamine (5 mg kg^?1 SC), and induced IV with ketamine (5 mg kg^?1) and diazepam (0.25 mg kg^?1). Serum biochemistry values were taken at 24 and 48 hours post‐surgically and compared to a pre‐surgical baseline. Behavioral data were collected by a blinded investigator prior to surgery (baseline) and 1, 2, 3, 4, 8, 12, 16, 20, and 24 hours post‐surgery; the data were compiled into composite pain scores on a scale from 0 to 21 and complemented by visual analogue scores (VAS). Scoring was based on changes in behavior, posture, vocalization, and response to interactive stimulation. Cats with pain scores >12 were considered to be moderately painful, received meperidine (4 mg kg^?1 IM), and were excluded from further statistical analyses. Sixty of 71 cats completed the study. Anesthetic time was 88.5 ± 21.8 minutes (mean ± SD). Meperidine was given to one cat in C, three in B, and five in S. There were no significant differences in biochemistry values. There were no significant differences in pain scores between C and B at any time period; B and C pain scores were significantly lower than S at 1, 2, 12, 16, and 20 hours post‐operatively, and C lower than S at 3 and 8 hours post‐surgery. Pain scores decreased over the 24‐hour study in all groups; the greatest decrease in each group was between 4 and 8 hours post‐operatively. In this study, carprofen provided post‐surgical analgesia comparable to butorphanol.     

Phenotypic and genetic parameter estimates for reproductive traits in Zandi sheep

This study reports on the phenotypic and genetic (co)variance components for reproductive traits in Zandi sheep, using between 1,859 and 2,588 records obtained from 577 ewes. The data were collected from the Khojir Breeding Station of Zandi sheep in Tehran, Iran from 1994 to 2008. The basic traits were litter size at birth (LSB), litter size at weaning (LSW), litter mean weight per lamb born (LMWLB), and litter mean weight per lamb weaned (LMWLW), and the composite traits were total litter weight at birth (TLWB) and total litter weight at weaning (TLWW). Genetic analyses were carried out using the restricted maximum likelihood method that was explored by fitting the additive direct genetic effects and permanent environmental effects of the ewes as random effects and the ewe age at lambing and lambing year as fixed effects for all of the investigated traits. Akaike’s information criterion was used to choose the most appropriate model. LSB, LSW, LMWLB, LMWLW, TLWB, and TLWW direct heritability estimates were 0.07, 0.05, 0.12, 0.10, 0.08, and 0.14, respectively. The estimated fractions of variance due to the permanent environmental effects of the ewe ranged from 0.03 for LMWLB to 0.08 for LMWLW and TLWW. Corresponding repeatability estimates ranged from 0.10 for LSW to 0.22 for TLWW. Direct genetic correlations varied from ?0.61 for LSB–LMWLB to 0.88 for LSB–LSW and LSB–TLWB. Results indicate that genetic change depends not only on the heritability of traits, but also on the observed phenotypic variation; therefore, improvement of non-genetic factors should be included in the breeding programs.     

Factors Affecting the First Service Conception Rate of Cows in Smallholder Dairy Farms in Bangladesh

The successful outcome of an insemination is a combination of both male and female fertility‐linked factors. We investigated the first service conception rate of cows at artificial insemination (AI) in the smallholder dairy farms in Bangladesh. Frozen straws were prepared from ejaculates of Bos indicus (n = 7) and Bos indicus × Bos taurus (n = 7) AI bulls. Fertility was determined from 6101 first services in cows that were performed by 18 technicians in four regions between April 2004 and March 2005. Pregnancy was diagnosed by rectal palpation between 60 and 90 days post‐insemination. The Asian version of Artificial Insemination Database Application (AIDA ASIA) was used for bulls‐, cows‐ and AI‐related data recording, and later retrieved for analysis. The mean ± SD number of inseminations performed from individual bulls and their conception rates were 436.0 ± 21.6 and 50.7 ± 1.9%, respectively. Logistic regression demonstrated body condition scores (BCS), heat detection signs, months of AI and their interactions had greatest effects (odds ratios: 1.24–16.65, p < 0.04–0.001) on first service conception rate in cows. Fertility differed (p < 0.02–0.001) between the regions, previous calving months, months of AI, BCS, parity and heat detection signs of cows. Inseminations based on mounting activity (n = 2352), genital discharge (n = 3263) and restlessness and/or other signs (n = 486) yielded a conception rate of 53.6%, 48.8% and 50.1%, respectively (p < 0.05). Conception rate between technicians ranged between 43.4% and 58.6% (p < 0.05). The days interval from calving to first service (overall mean ± SD = 153.4 ± 80.6) had relationship (p < 0.001) with BCS, months of previous calving and parity of the cows. Fertility at AI in smallholder farms can be improved by training farmers on nutrition and reproductive management of the cows.     

Toxicity of selected insecticides to the two-spot ladybird <Emphasis Type="Italic">Adalia bipunctata</Emphasis>

The toxicity of pirimicarb, imidacloprid, dimethoate, lambda-cyhalothrin, flonicamid and spinosad to the two-spot ladybird, Adalia bipunctata, was evaluated in a laboratory study. Susceptibility of fourth instars and female adults was assessed by measuring toxicity via residual contact and ingestion through feeding on contaminated green peach aphids (Myzus persicae). Flonicamid and spinosad had no lethal effects on larvae and female adults. Pirimicarb was harmless to the predator by ingestion exposure but showed some residual toxicity at high concentrations to both larval and adult stages. Imidacloprid was highly toxic to the larval stage by residual and ingestion exposure but caused very low adult mortality when ingested through contaminated prey. Dimethoate and lambda-cyhalothrin were highly toxic to both the larval and adult stages of the ladybird. Our findings indicate that pest management programs in agricultural crops using dimethoate, lambda-cyhalothrin and, to a lesser degree, imidacloprid, are detrimental to A. bipunctata, whereas pirimicarb, flonicamid and spinosad are more compatible with the use of this predator.     

Evaluation of seedling and adult plant resistance in European wheat cultivars to Australian isolates of <Emphasis Type="Italic">Puccinia striiformis</Emphasis> f. sp. <Emphasis Type="Italic">tritici</Emphasis>

A set of 105 European wheat cultivars was assessed for seedling resistance and adult plant resistance (APR) to stripe (yellow) rust in greenhouse and field tests with selected Australian isolates of Puccinia striiformis f. sp. tritici (Pst). Twelve cultivars were susceptible to all pathotypes, and among the remainder, 11 designated seedling genes (Yr1, Yr3, Yr4, Yr6, Yr7, Yr9, Yr17, Yr27, Yr32, YrHVII and YrSP) and a range of unidentified seedling resistances were detected either singly or in combination. The identity of seedling resistance in 43 cultivars could not be determined with the available Pst pathotypes, and it is considered possible that at least some of these may carry uncharacterised seedling resistance genes. The gene Yr9 occurred with the highest frequency, present in 19 cultivars (18%), followed by Yr17, present in 10 cultivars (10%). Twenty four cultivars lacked seedling resistance that was effective against the pathotype used in field nurseries, and all but two of these displayed very high levels of APR. While the genetic identity of this APR is currently unknown, it is potentially a very useful source of resistance to Pst. Genetic studies are now needed to characterise this resistance to expedite its use in efforts to breed for resistance to stripe rust. Colin R. Wellings seconded from NSW Department of Primary Industries.     

Factors Affecting the Selection of Information Sources of Sustainable Agricultural Practices by Malaysian Vegetable Farmers

Information is key in reducing the uncertainties of sustainable agricultural practices (SAPs). The objective of this study was to improve the understanding of the relationship between utilized informational sources and other key identified factors affecting Malaysian vegetable producers' choices. The findings of a multivariate probit model suggest that information sources are complementary. The choices for these sources are influenced by heterogeneity in access to credit, social network (member of farmers association), and farm settings (distance from farm to market, resource depletion issues, and geographical regions). Future promotion of SAPs should be delivered through the provision of quality content disseminated through the preferred information sources, which target potential users.     

Environmental and maternal factors associated with gross placental morphology in dairy cattle

This article reports on a study of gross placental morphology of 282 expelled placentas from 89 primi‐ and 193 multiparous Holstein dams immediately after calving and examines associations with environmental factors such as typical herd features and season of calving, and maternal factors such as age at calving, level of milk yield at conception and cumulative amount of milk produced during gestation. The highest correlation between calf measurements and placental characteristics was found between the weight of the calf and the total cotyledonary surface (r = .643; p < .001), confirming the high importance of the cotyledonary surface available for nutrient transfer to the developing foetus. Younger age in adolescent and smaller heart girth in multiparous dams were associated with a higher cotyledon number, suggesting placental compensation in dams with lower capacities in terms of dry matter intake. No significant association between milk yield during gestation in multiparous animals and gross placental morphometrics could be detected, indicating that factors such as the amount of milk produced during gestation affect placental development less than foetal weight close to term. Therefore, placental growth may be sustained at the expense of other tissues in an attempt to maintain pregnancy and minimize the adverse consequences for the foetus. This study offers evidence concerning factors affecting the placental surface size for nutrient transfer from dam to calf in dairy cattle based on gross morphometrics, but needs confirmation from studies in which this surface size is more profoundly assessed by measuring the branches present in the cotyledonary villi.     

Nutritional status of Washington Navel orange orchards in arid lands of southern Iran

Nutrient deficiency may be a reason for citrus decline in many countries. In the current investigation, soil and leaf samples of 63 Washington Navel orange orchards in arid lands of southern Iran (Darab region) were analyzed to study the nutritional status of orange orchards and to find relationships between soil properties and nutrients content of soils and orange leaves. The soils differed widely in clay content (3–37%), pH value (7.04–7.95), calcium carbonate equivalent (18–86%), and organic matter content (0.3–12%). These soils represented a wide range of plant nutrients concentration. Majority of the studied soils had sufficient concentrations of all nutrients (except Fe). Results indicated that pH, organic matter, clay, and calcium carbonate contents are the most important characteristics that control the soil nutrients availability. The mean contents of N, K, P, Fe, Mn, Zn, and Cu in leaves were 2.59, 0.84, and 0.14% and 66, 44, 17, and 12?mg?kg^?1, respectively. Most orchards showed K, Fe, and Zn deficiencies. We found no relationships between nutrients content in leaf and soil (except for K, Mn, and Zn); and this indicated that soil analysis may not be a reliable method for most nutrient deficiency diagnosis.