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71.
Hypothyroidism Affects Differentially the Cell Size of Epithelial Cells Among Oviductal Regions of Rabbits 下载免费PDF全文
A Anaya‐Hernández J Rodríguez‐Castelán L Nicolás M Martínez‐Gómez I Jiménez‐Estrada F Castelán E Cuevas 《Reproduction in domestic animals》2015,50(1):104-111
Oviductal regions show particular histological characteristics and functions. Tubal pathologies and hypothyroidism are related to primary and secondary infertility. The impact of hypothyroidism on the histological characteristics of oviductal regions has been scarcely studied. Our aim was to analyse the histological characteristics of oviductal regions in control and hypothyroid rabbits. Hypothyroidism was induced by oral administration of methimazole (MMI) for 30 days. For both groups, serum concentrations of thyroid and gonadal hormones were determined. Sections of oviductal regions were stained with the Masson's trichrome technique to analyse both epithelial and smooth muscle layers. The percentage of proliferative epithelial cells (anti‐Ki67) in diverse oviductal regions was also quantified. Data were compared with Student t‐test, Mann–Whitney U‐test, or Fischer's test. In comparison with the control group, the hypothyroid group showed: (i) a low concentration of T3 and T4, but a high level of TSH; (ii) similar values of serum estradiol, progesterone and testosterone; (iii) a large size of ciliated cells in the ampulla (AMP), isthmus (IST) and utero‐tubal junction (UTJ); (iv) a large size of secretory cells in the IST region; (v) a low percentage of proliferative secretory cells in the fimbria‐infundibulum (FIM‐INF) region; and (vi) a similar thickness of the smooth muscle layer and the cross‐sectional area in the AMP and IST regions. Modifications in the size of the oviductal epithelium in hypothyroid rabbits could be related to changes in the cell metabolism that may impact on the reproductive functions achieved by oviduct. 相似文献
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Pacemakers are commonly implanted by veterinary cardiologists in dogs with bradyarrhythmias. In the past, these pacemakers were placed with little attention to programming except for the basic heart rate. However, the availability of pacing programmers has permitted a better appreciation of the needs for proper and safe pacing in dogs. An awareness of the problems that can result from improper programming is imperative. We report the identification of noise reversion (inappropriate asynchronous pacing) in 42% of dogs consecutively paced (n = 19) at Cornell University during a 2 year period. Noise reversion is the operation that causes the pacemaker to switch to asynchronous pacing with repetitive refractory sensing. It was developed as a protective mechanism against noise being mistaken for cardiac events with the consequence of inhibition of pacing. However, in the dog, this operation can cause inappropriate, and potentially dangerous pacing. In the dog, this common complication is associated with the coexistence of a bradycardia and tachycardia. Proper programming of the refractory period (shortening) in conjunction with the sensitivity (decreasing the sensitivity) can eliminate noise reversion in dogs with both ventricular and atrial pacing. 相似文献
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Amara RR Villinger F Altman JD Lydy SL O'Neil SP Staprans SI Montefiori DC Xu Y Herndon JG Wyatt LS Candido MA Kozyr NL Earl PL Smith JM Ma HL Grimm BD Hulsey ML Miller J McClure HM McNicholl JM Moss B Robinson HL 《Science (New York, N.Y.)》2001,292(5514):69-74
Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster controlled a highly pathogenic immunodeficiency virus challenge in a rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the acquired immune deficiency syndrome epidemic. 相似文献
77.
The effectiveness of selecting umbrella species or groups of species to protect other groups is a key and unresolved issue in conservation planning. We detected areas important to the four groups of terrestrial vertebrates inhabiting Andalusia (southern Spain) according to four conservation-value criteria, i.e., richness, rarity, vulnerability, and endemicity. We calculated coincidences between criteria and taxa by the degree of correlation and fuzzy overlap between the different criteria for the same group (intra-group coincidence) and between the different groups of species for the same criteria (inter-group coincidence). For the groups to be appropriate umbrella species, inter-group coincidence should be high. The results showed that intra-group coincidence was high, but inter-group coincidence was low. Thus, zones of importance to each group of species tended to be located in different places compared to the other groups, and therefore none of them should be used as an indicator or umbrella group to protect the others. This result has implications for conservation policy, as the Andalusian authorities should not assume that protecting a site important to one group of species will also be relevant to conserve the other groups. Nevertheless, we identified the areas of relatively high conjoint importance for all the groups, and the best umbrella group in this region, which proved to be reptiles. 相似文献
78.
Effect of segregated early weaning on postweaning small intestinal development in pigs 总被引:5,自引:0,他引:5
Tang M Laarveld B Van Kessel AG Hamilton DL Estrada A Patience JF 《Journal of animal science》1999,77(12):3191-3200
The effect of segregated early weaning (SEW) on postweaning small intestinal development was investigated in SEW and control (CON) pigs. Small intestines were collected from a total of 15 pigs killed at 11 (preweaning), 15 (3 d postweaning), and 34 d of age. At 3 d postweaning, the SEW and CON pigs had shorter villi (P<.01), deeper crypts (P<.01), and reduced (P<.01) ratios of villus height:crypt depth (V:C) compared with preweaning. Weaning also reduced specific activities of lactase (P<.01) in duodenum and ileum and alkaline phosphatase (ALP) (P<.05) in duodenum and jejunum. Sucrase activity in the three regions of the small intestine marginally decreased in both groups at 3 d postweaning. The mucosal protein:DNA ratio in duodenum and jejunum increased (P<.05) in SEW and CON pigs at 3 d postweaning compared with preweaning pigs. The SEW and CON treatments resulted in differences in postweaning gut development. At 15 d of age in SEW pigs, the mucosal protein:DNA ratio in duodenum and jejunum were 20 and 25.5% (P<.05) less, respectively, than those in CON pigs. However, at 34 d, these ratios in duodenum, jejunum, and ileum were 43.5 (P<.05), 24.3, and 32.9% (P<.05) greater, respectively, in SEW pigs than in CON pigs. Longer villi, shorter crypts (P<.01), and higher V:C ratios (P<.01) in jejunum and ileum were observed in SEW pigs vs CON pigs at 34 d of age. The specific activities of lactase in duodenum (P<.01) and jejunum (P<.05) and of ALP in duodenum (P<.01) were higher in SEW pigs. Sucrase activity in duodenum, jejunum, and ileum was 21.7, 46.3 (P<.05), and 11.2% greater in SEW pigs at 34 d of age. These results demonstrate differences in postweaning gut development between SEW and CON pigs. Furthermore, the number of intraepithelial lymphocytes in jejunum was greater (P<.001) in 34-d-old SEW pigs compared with CON pigs. Microscopy revealed a thick mucus coating over epithelial cells in the ileum of 34-d-old CON pigs that was not apparent in the SEW pigs. These observations are consistent with reduced pathogen exposure associated with SEW. We suggest that segregated early weaning advances postweaning gut maturation, which is consistent with improved growth and feed efficiency observed in SEW pigs. 相似文献
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Nuala Summerfield MRCVS Dipl ACVIM Amara Estrada DVM Dipl ACVIM 《Journal of Veterinary Cardiology》2005,7(2):131-135
Ladder diagrams are valuable teaching and interpretive tools in both human and veterinary electrocardiography. The purpose of this paper is to demonstrate how ladder diagrams can be used to help understand the possible mechanisms underlying atrial flutter and atrial fibrillation. 相似文献
80.
Electrocardiography in two subspecies of manatee (Trichechus manatus latirostris and T. m. manatus).
Jessica Siegal-Willott Amara Estrada Robert Bonde Arthur Wong Daniel J Estrada Kendal Harr 《Journal of zoo and wildlife medicine》2006,37(4):447-453
Electrocardiographic (ECG) measurements were recorded in two subspecies of awake, apparently healthy, wild manatees (Trichechus manatus latirostris and T. m. manatus) undergoing routine field examinations in Florida and Belize. Six unsedated juveniles (dependent and independent calves) and 6 adults were restrained in ventral recumbency for ECG measurements. Six lead ECGs were recorded for all manatees and the following parameters were determined: heart rate and rhythm; P, QRS, and T wave morphology, amplitude, and duration; and mean electrical axis (MEA). Statistical differences using a t-test for equality of means were determined. No statistical difference was seen based on sex or subspecies of manatees in the above measured criteria. Statistical differences existed in heart rate (P = 0.047), P wave duration (P = 0.019), PR interval (P = 0.025), and MEA (P = 0.021) between adult manatees and calves. Our findings revealed normal sinus rhythms, no detectable arrhythmias, prolonged PR and QT intervals, prolonged P wave duration, and small R wave amplitude as compared with cetacea and other marine mammals. This paper documents the techniques for and baseline recordings of ECGs in juvenile and adult free-living manatees. It also demonstrates that continual assessment of cardiac electrical activity in the awake manatee can be completed and can be used to aid veterinarians and biologists in routine health assessment, during procedures, and in detecting the presence of cardiac disease or dysfunction. 相似文献