Physiologic responses and plasma endothelin-1 concentrations associated with abrupt cessation of nitric oxide inhalation in isoflurane-anesthetized horses

OBJECTIVE: To assess physiologic responses and plasma endothelin (ET)-1 concentrations associated with abrupt cessation of nitric oxide (NO) inhalation in isoflurane-anesthetized horses. ANIMALS: 6 healthy adult Standardbreds. PROCEDURES: Horses were anesthetized with isoflurane in oxygen and placed in dorsal recumbency. Nitric oxide was pulsed into the respiratory tract for 2.5 hours, and then administration was abruptly discontinued. Just prior to commencement and at cessation of NO administration, and at intervals during a 30-minute period following cessation of NO inhalation, several variables including PaO(2), mean pulmonary artery pressure, venous admixture or pulmonary shunt fraction (Qs/Qt), and plasma ET-1 concentration were recorded or calculated. RESULTS: After cessation of NO inhalation, PaO(2) decreased slowly but significantly (172.7 +/- 29.8 mm Hg to 84.6 +/- 10.9 mm Hg) and Qs/Qt increased slowly but significantly (25 +/- 2% to 40 +/- 3%) over a 30-minute period. Mean pulmonary artery pressure increased slightly (14.0 +/- 1.3 mm Hg to 16.8 +/- 1 mm Hg) over the same time period. No change in serum ET-1 concentration was detected, and other variables did not change or underwent minor changes. CONCLUSIONS AND CLINICAL RELEVANCE: The improvement in arterial oxygenation during pulsed inhalation of NO to healthy isoflurane-anesthetized horses decreased only gradually during a 30-minute period following cessation of NO inhalation, and serum ET-1 concentration was not affected. Because a rapid rebound response did not develop, inhalation of NO might be clinically useful in the treatment of hypoxemia in healthy isoflurane-anesthetized horses.     

A comparative analysis of water application and energy consumption at the irrigated field level

Most government policies and community perception of the irrigation sector promotes the conversion from gravity-fed to pressurised irrigation methods as a way of reducing water consumption by the irrigation sector. However, optimising for one aspect of a system can have unintended resource and environmental consequences e.g. an increase in energy consumption patterns of irrigated crops. Two Australian irrigation areas were studied: a surface-water supplied region in New South Wales; and a groundwater dependent region in South Australia. The water and energy budgets for crop production from land preparation to harvest were quantified on several farms. Converting from flood to pressurised systems resulted in a reduction in water application of between 10% and 66%. However, in the surface-water supplied region, it also resulted in energy consumption being increased by up to 163%. In the groundwater dependent region, energy consumption was reduced by 12% to 44%. There is potential to reduce energy consumption due to increased water use efficiency, resulting in less water being pumped due to efficiency gains. Therefore, to optimise energy and water use, it is recommended that pressurised irrigation systems be used in areas requiring pressurised extraction of groundwater, while efficient gravity based irrigation methods, coupled with good management practices, be promoted in surface-water supplied areas.     

Fine‐scale population structure and connectivity of bottlenose dolphins,Tursiops truncatus,in European waters and implications for conservation

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East Java Propolis Inhibits Cytokine Pro-inflammatory in Odontoblast-Like Cells Human Pulp

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Plant cuticular lipid export requires an ABC transporter

A waxy protective cuticle coats all primary aerial plant tissues. Its synthesis requires extensive export of lipids from epidermal cells to the plant surface. Arabidopsis cer5 mutants had reduced stem cuticular wax loads and accumulated sheetlike inclusions in the cytoplasm of wax-secreting cells. These inclusions represented abnormal deposits of cuticular wax and resembled inclusions found in a human disorder caused by a defective peroxisomal adenosine triphosphate binding cassette (ABC) transporter. We found that the CER5 gene encodes an ABC transporter localized in the plasma membrane of epidermal cells and conclude that it is required for wax export to the cuticle.     

Genetic diversity of rhizobia associated with indigenous legumes in different regions of Flanders (Belgium)

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