Development of a Non-radioactive Dot-blot Hybridisation Assay for the Detection of Pelargonium Flower Break Virus and Pelargonium line Pattern Virus

The ornamental geranium, Pelargonium×hortorum Bailey, is a traditional ornamental plant widely cultivated in Europe and Northern America. Vegetative propagation facilitates rapid spread of viral infections which have detrimental effects on the production and the quality of the crop. A non-radioactive nucleic acid hybridisation method was developed for detection of Pelargonium flower break virus (PFBV) and Pelargonium line pattern virus (PLPV) in infected host plants. This method was significantly more sensitive than the conventional ELISA test when using either purified viral preparations or crude plant extracts. The distribution of the viruses was studied by means of the non-isotopic hybridisation technique. The results indicated that the petioles and the apical blade regions of fully expanded leaves were the best source of test material. The hybridisation procedure enables the detection of PFBV and PLPV in a single assay, and its simplicity allows its application to routine large-scale indexing.     

Mastitis caused by Mycoplasma mycoides subspecies mycoides (large colony type) in goat flocks in Spain

We describe three different outbreaks of mastitis caused by M. mycoides subspecies mycoides LC type (Mmm LC) in three goat flocks from the Extremadura Region of south-west Spain. Thirty-two fast-growing isolates were obtained on Hayflick's and Friis's media with inhibitors from different specimens. All were identified as Mmm LC in spite of their cultural, biochemical and serological features.     

Lumber volume modeling of Amazon Brazilian species

Accurate estimates of lumber volume become an important indicator of production and monetary value for a sawmill. However, such estimates are only obtained directly after logging, and no accurate predictions are made for the Amazonian commercial species. In this sense, the objective was to generate equations of lumber volume for commercial species in Amapá (a state in northern Brazil) by adjusting and selecting regression models. The data of 50 logs processed from 10 commercial species were collected, as well as the quantity and volume of sawn products. Sixteen (16) statistical models were adjusted and statistical weights were performed to evaluate the quality of the estimates and to select the best equation by species. In summary, precise estimates of lumber volume can be obtained by the nº16 model for Carapa guianensis, while the models nº13 and nº15 are the most recommended for Dinizia excelsa and Hymenolobium petraeum, respectively. Model 7 presented the best adjustments for Hymenaea courbaril and Vochysia guianensis. Equations using only the log diameter variable suggest less precise estimates. Also, the log volume should be considered as an important predictor variable to obtain the serrated/lumber volume for the different Amazonian commercial species.     

Insecticide resistance, mixture potentiation and fitness in populations of the maize weevil (Sitophilus zeamais)

High levels of pyrethroid resistance and emerging organophosphate resistance in Brazilian populations of the maize weevil Sitophilus zeamais (Coleoptera: Curculionidae) led to the registration of esfenvalerate + fenitrothion against them. Thus, a survey of physiological and behavioural resistance was carried out in 27 insect populations for two pyrethroids, esfenvalerate and permethrin, and the esfenvalerate + fenitrothion mixture. Physiological resistance to fenitrothion was also assessed, as was the potentiation of the mixture. The potential fitness cost associated with insecticide resistance was also investigated. The resistance levels were low to fenitrothion (<14.1-fold), low to moderate to the pyrethroids (1.6–70.0-fold) and low to the pyrethroid-organophosphate mixture (<5-fold) with a high heterogeneity of response among populations. The potentiation of insecticidal activity achieved with the insecticide mixture was very high (>350-fold) reinforcing its usefulness for managing weevils. There was little variation in walking behaviour (and insecticide avoidance) among populations; there was no significant variation in fitness, body mass and respiration rate among the populations of the insect. These however, displayed variable rates of grain consumption and activity of amylase and lipase. A decrease in insecticide resistance in maize weevil populations was observed relative to previous studies, but with an initial development of resistance to the insecticide mixture. Behavioural and physiological resistance were not correlated and not associated with fitness cost. Our results support the use of esfenvalerate + fenitrothion against the maize weevil, which is likely to have reduced the levels of pyrethroid resistance in field populations. However, resistance to this mixture seems to be evolving, justifying concerns regarding its use.     

A comparison of energy use in conventional and organic olive oil production in Spain

The current situation of worldwide concern over the emission of greenhouse gases and its effect on the climate demands an evaluation, from the perspective of energy efficiency and more specifically of non-renewable energy sources, of tendencies for change in the management of agricultural systems which have arisen in recent years. This article uses energy balances to evaluate the contribution of organic olive growing to the increase in the energy efficiency of Mediterranean agriculture, distinguished according to type of watering regime and intensiveness of cultivation. The results show, on one side, the lower energy efficiency of irrigated land as opposed to dryland (i.e. non-irrigated) regardless of their style of management and, on the other, the greater non-renewable energy efficiency of organic olive growing in comparison with the conventional production. Nevertheless, organic management could still improve its energy efficiency if it further adjusts and internalizes the flows of nutrients needed in order to achieve greater sustainability.     

Diversity and pathogenicity of Colletotrichum species isolated from soursop in Colombia

Anthracnose, caused by Colletotrichum species is a highly limiting disease for the production of the tropical fruit tree crop, soursop (Annona muricata L.). In this study, 83 single-spore isolates of Colletotrichum were obtained from diseased soursoup tissues and subjected to a species complex-specific PCR assay. The isolates were identified as C. gloeosporioides sensu lato (n?=?60), C. boninense s. lat. (n?=?22), or C. acutatum s. lat. (n?=?1). A subset of 21 selected isolates was identified to species level by means of a multi-locus phylogenetic analysis using sequences from the ITS region and partial sequences of the actin, β-tubulin-2, glyceraldehyde-3-phosphate dehydrogenase, and chitin synthase-1 genes. The multi-locus phylogenetic analysis resolved C. theobromicola, C. tropicale, C. siamense, and C. gloeosporioides sensu stricto in the C. gloeosporioides complex; C. karstii and one undetermined species in the C. boninense complex; as well as one undetermined species in the C. acutatum complex. Significant differences in anthracnose severity were observed between Colletotrichum species when tested for pathogenicity on attached twigs of soursop cv. Elita. Colletotrichum theobromicola and C. tropicale were associated with high and intermediate virulence, respectively, whereas the remaining species were associated with low virulence.