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121.
Benjamin Lopez-Jimena Esther Garcia-Rosado Kim Dawn Thompson Alexandra Adams Carlos Infante Juan Jose Borrego Maria del Carmen Alonso 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(4):355-362
The distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens was examined by immunohistochemistry in the nervous and non-nervous organs of juvenile European seabass (Dicentrarchus labrax) during the course of an intramuscular infection. Histological changes resulting from the infection were evaluated from 3 days to 2 months post-infection. The specific antibody response was also studied 2 months post-challenge. Viral proteins were present throughout the experimental period in the retina (inner nuclear layer, ganglion layer, outer limiting membrane, and outer plexiform layer), brain (cerebellum and tectum opticum), and liver (hepatocytes and endothelial cells). These proteins were also observed in the renal tubular cells, white pulp of spleen, and in fibroblasts and cartilage of caudal fin. This is the first report of RGNNV proteins appearing in these organs, where the immunostaining was only detected at certain sampling times after the onset of mortality. Brain and retina of virus-exposed fish showed high levels of vacuolation, while accumulation of fat vacuoles was observed in the liver. RGNNV infection also induced a specific antibody response as measured by an ELISA. In summary, this is the first study demonstrating the presence of viral proteins in cells of caudal fin, kidney and spleen of European seabass. 相似文献
122.
Biological control of postharvest diseases of fruit 总被引:1,自引:0,他引:1
Carla Alexandra Nunes 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(1):181-196
Postharvest decay in harvested fruit causes considerable economical losses. Fungicides are the primary means to control these
losses. Public concern in food safety and environmental issues and the increase of pathogen resistant populations have enhanced
the interest in developing alternative methods to fungicides to control postharvest fruit decay. During the last two decades
a huge information and advances concerning the selection of antagonists, mode of action, different approaches to enhance biocontrol
activity, formulation and production have been achieved, and some biofungicides are already in the market. It is likely that
several more products will enter the market in the near future, as the result of the biological control research programs
worldwide. Nonetheless, it is necessary to continue finding new potential microorganisms, better understanding the mode of
action, and pathogen, antagonist and host interactions, to increase the potential of biocontrol helping to become a real alternative
to synthetic postharvest fungicides. This article presents an overview of postharvest biological control approaches and explores
new research possibilities to improve biocontrol activity. 相似文献
123.
Steinberg TA Boettcher IC Matiasek K Hirschvogel K Hartmann K Kunz A Kuntz A Fischer A 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(2):207-216
BACKGROUND: Inflammation of the central nervous system (CNS) is a frequent condition in cats but etiology often remains unsolved. Routine cerebrospinal fluid (CSF) analysis can be extended through the calculation of the albumin quotient (Q(alb)), a marker of the integrity of the blood-brain barrier (BBB), and IgG index, an estimate of intrathecal IgG synthesis. OBJECTIVES: The purpose of this study was to validate nephelometric methods for CSF protein analysis, and to use the Q(alb) and IgG index to discriminate blood- and brain-derived immunoglobulin fractions in cats with feline infectious peritonitis (FIP). METHODS: Cats presented to our clinic between 2001 and 2005 were included in the study based on clinical and laboratory data and histopathologic findings at necropsy. Cats were grouped as having nonneurologic disease (controls; n=37), brain tumors (n=8), FIP involving the CNS (n=12), and extraneural FIP (n=12). CSF-total protein (TP) was measured and albumin and IgG concentrations were measured in paired CSF/serum samples; Q(alb) and IgG index were calculated. Intraassay and interassay precision of the nephelometric assays were determined using pooled samples. RESULTS: Coefficients of variation for the nephelometric assays ranged from 2.7% to 7.2%. In control cats, CSF-TP concentration ranged from 0.06 to 0.36 g/L, Q(alb) ranged from 0.6 to 5.7 x 10(-3), and IgG index ranged from 0.3 to 0.6. Q(alb) and IgG index were significantly higher in cats with brain tumors and cats with CNS-FIP compared with other groups. Compared with control cats, pleocytosis was evident in 8 of 12 (67%) cats and CSF-TP was increased in 3 of 12 (25%) cats with CNS-FIP. CONCLUSION: Nephelometry is a reliable method for measurement of CSF protein, albumin, and IgG in cats. The Q(alb) and IgG index did not identify a CSF protein pattern specific for BBB dysfunction or intrathecal IgG synthesis in cats with CNS-FIP. 相似文献
124.
Use of personal protective equipment in a radiology room at a veterinary teaching hospital 下载免费PDF全文
Monique N. Mayer Niels K. Koehncke Alexandra F. Belotta Isaac T. Cheveldae Cheryl L. Waldner 《Veterinary radiology & ultrasound》2018,59(2):137-146
The use of personal protective equipment by veterinary workers during radiographic imaging is inconsistent. While the self‐reported use of leaded aprons and thyroid shields approaches 100% in some studies, the use of leaded gloves and eyeglasses is much lower. Previous studies describing personal protective equipment use are based on self‐reporting. Objectives of this prospective, observational study were to describe use of leaded personal protective equipment during radiographic imaging by veterinary workers, and to compare observed use with self‐reported use. Use of leaded personal protective equipment during radiographic imaging by veterinary workers was observed over a 10 week period using two motion‐triggered video cameras, and a questionnaire was then completed by workers on their use of personal protective equipment. Workers restrained the animal during 91.8% (753/820) of exposures. An apron and a securely closed thyroid shield were worn for >99% of studies. Gloves were used correctly for 43.6% (156/358) of radiographic studies. Leaded eyeglasses were worn for 1.7% (6/358) of studies. Correct glove use was more frequent during regular working hours than after‐hours for both veterinarians (odds ratio 32.7, P = 0.001) and veterinary students (odds ratio 75.1, P < 0.001). The number of workers in the room was lower when animals were sedated (P = 0.002) or anesthetized (P = 0.017). Workers overestimated their frequency of glove use (P <0.001). In conclusion, workers use personal protective equipment less frequently in an unsupervised environment, and overestimate their use of personal protective equipment. Use of sedation or anesthesia decreases worker exposure to ionizing radiation. 相似文献
125.
Jorge Durán Alexandra Rodríguez José María Fernández-Palacios Antonio Gallardo 《Biology and Fertility of Soils》2009,45(7):781-788
The concern that climate change may increase fire frequency and intensity has recently heightened the interest in the effects
of wildfires on ecosystem functioning. Although short-term fire effects on forest soils are well known, less information can
be found on the long-term effects of wildfires on soil fertility. Our objective was to study the 17-year effect of wildfires
on forest net mineralization rates and extractable inorganic nitrogen (N) and phosphorus (P) concentrations. We hypothesize
that (1) burned forest stands should exhibit lower net mineralization rates than unburned ones; (2) these differences would
be greatest during the growing season; (3) differences between soil variables might also be observed among plots from different
years since the last fire; and (4) due to fire-resistant geochemical processes controlling P availability, this nutrient should
recover faster than N. We used a wildfire chronosequence of natural and unmanaged Pinus canariensis forests in La Palma Island (Canary Islands). Soil samples were collected during winter and spring at 22 burned and unburned
plots. We found significantly higher values for net N mineralization and extractable N pools in unburned plots. These differences
were higher for the winter sampling date than for the spring sampling date. Unlike extractable N and N mineralization rates,
extractable P levels of burned plots exhibited a gradual recovery over time after an initial decrease. These results demonstrate
that P. canariensis forest soils showed low resilience after wildfires, especially for N, and that this disturbance might induce long-term changes
in ecosystem functioning. 相似文献
126.
J Traub-Dagartz W Bayly M Riggs N Thomas R Pankowski 《Journal of the American Veterinary Medical Association》1985,186(3):280-281
An Arabian foal with a congenital heart disease died due to hemorrhage secondary to a large gastric ulcer. Previously, death of foals with gastric ulcers has been due to diffuse peritonitis resulting from gastric ulcer perforation. The foal in this case report died due to hemorrhage secondary to a large gastric ulcer. 相似文献
127.
128.
Urs P. Kreuter Dianne A. Stroman Carissa L. Wonkka John Weir Alexandra A. Abney James K. Hoffman 《Strength and Conditioning Journal》2019,72(6):959-967
Suppression of fire in the Southern Plains has led to proliferation of woody plants and fuel load accumulation that spurs wildfires. These effects have led to calls for widespread application of prescribed fire to reduce fuel loads, but there is substantial landowner resistance to the use of this land management tool. Here we explore factors that affect perceptions of landowners in the Southern Plains about prescribed fire liability and their willingness to apply this land management tool. This region was selected for the study because of the preponderance of private landholdings and widespread woody plant encroachment. The study used a mail survey of 1 853 landowners in 16 counties in Texas and Oklahoma, resulting in a data set from 680 respondents (37% useable response rate). Logistic regression models were developed to test three hypotheses relating to the likelihood that a landowner will apply prescribed fire. The study corroborated that landowners who perceived higher levels of fire-related legal liability were less likely to apply prescribed fire on their land or assist with its application on other properties. In addition, burn bans were found to inhibit landowner willingness to apply fire during periods that result in higher woody plant mortality. Oklahoma respondents, landowners who believed prescribed fire to be an affordable woody plant management tool, and members of prescribed burning associations (PBAs) were more likely to use prescribed fire. These results have important implications for policies aimed at overcoming resistance to the use of prescribed fire to curb woody plant encroachment and reduce fuel load accumulation. Specifically, language in state statutes pertaining to prescribed fire should be modified to reduce landowner concerns over legal liability; PBAs should be established more widely; and public cost-sharing funds for woody plant management should prioritize prescribed fire. 相似文献
129.
Valentin Janvier Laurence Evrard Simona Cerri Alexandra Gougnard Valeria Busoni 《Veterinary radiology & ultrasound》2016,57(1):65-74
Ultrasonography and radiography are commonly used for staging of lymphoma in horses, however there is little published information on imaging characteristics for horses with confirmed disease. The purpose of this retrospective, case series study was to describe ultrasonographic and radiographic findings for a group of horses with a confirmed diagnosis of lymphoma. A total of 13 horses were sampled. Lymphadenopathy (8/13), peritoneal effusion (6/13), splenic (6/13), and hepatic (5/13) lesions were the most frequently identified. The predominant splenic and hepatic ultrasonographic lesions were hypoechoic nodules, organomegaly, and changes in echogenicity. Digestive tract lesions were detected in three horses and these included focal thickening and decreased echogenicity of the small (2/13) and large intestinal (2/13) wall. Thoracic lesions were predominantly pleural effusion (4/13), lymphadenopathy (4/13), and lung parenchymal changes (3/13). Enlarged lymph nodes were detected radiographically (4/13) and/or ultrasonographically (2/13) in the thorax and ultrasonographically in the abdomen (7/13) and in the caudal cervical region (4/13). Findings supported the use of abdominal and thoracic ultrasonography for lymphoma staging in horses. Ultrasound landmarks for localizing cecal and caudal deep cervical lymph nodes were also provided. 相似文献
130.
Reconstruction of a Complicated Orbital Depression Fracture with Medial Wall and Globe Repositioning in a Horse: A Collaboration Across Disciplines and Specialties 下载免费PDF全文