全文获取类型
收费全文 | 452篇 |
免费 | 48篇 |
专业分类
林业 | 24篇 |
农学 | 10篇 |
基础科学 | 3篇 |
86篇 | |
综合类 | 30篇 |
农作物 | 21篇 |
水产渔业 | 34篇 |
畜牧兽医 | 232篇 |
园艺 | 12篇 |
植物保护 | 48篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 15篇 |
2021年 | 27篇 |
2020年 | 31篇 |
2019年 | 29篇 |
2018年 | 25篇 |
2017年 | 20篇 |
2016年 | 25篇 |
2015年 | 14篇 |
2014年 | 13篇 |
2013年 | 23篇 |
2012年 | 40篇 |
2011年 | 44篇 |
2010年 | 25篇 |
2009年 | 16篇 |
2008年 | 30篇 |
2007年 | 22篇 |
2006年 | 19篇 |
2005年 | 16篇 |
2004年 | 19篇 |
2003年 | 10篇 |
2002年 | 13篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1993年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有500条查询结果,搜索用时 0 毫秒
31.
Weel KG Boelrijk AE Burger JJ Verschueren M Gruppen H Voragen AG Smit G 《Journal of agricultural and food chemistry》2004,52(21):6564-6571
This paper describes a novel device to simulate in vivo aroma release from liquids. This artificial throat simulates the act of swallowing followed by exhalation and shows aroma release curves that are similar in shape to in vivo release profiles. Liquids are poured down a tube, and a thin liquid film remains at the inner wall of the tube. Subsequently, aroma compounds release from this film into a stream of air flowing through this tube, which is analyzed by MS-Nose analysis. The effects of air flow rate, contact time with glass surface, presence of saliva, and addition of whey protein, as well as volume, concentration, temperature, and viscosity of the liquid have been studied and compared with aroma release measurements in vivo. A high level of agreement was found. These results confirm the importance of swallowing for aroma release of liquids, as mentioned in the literature, and the usefulness of the new mimicking device. 相似文献
32.
Nunes A Correia I Barros A Delgadillo I 《Journal of agricultural and food chemistry》2005,53(3):639-643
Quantitative and qualitative analysis of uncooked zein and kafirin fractions were performed through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrophoretic profiles. Kafirins and zeins present the same oligomer and monomer compositions with the exception of a 66 kDa oligomer that is only present in kafirins. The quantitative analysis showed differences between zein and kafirin. The composition of each oligomer was established via preparative SDS-PAGE. Part of the cooked oligomers resists reduction; the presence of those oligomers could be related to the decrease on protein digestibility with the cooking process. 相似文献
33.
Jurate Virkutyte Emiliya Velizarova Alexandra B. Ribeiro Mika SillanpÄÄ 《Water, air, and soil pollution》2005,160(1-4):27-39
Chromated copper arsenate (CCA)-treated timber waste is hazardous for the environment due to the leakage of copper, chromium as well as arsenic to the soil and the groundwater. The study concentrates on electrodialytic removal of copper and chromium and their migration behavior in the wood chips and sawdust matrix in an applied electric field. The method is based on the movement of the charged particles in the electric field, enhanced by incubation with oxalic acid (2.5% w/w) and various placement of the contaminated waste in the electrodialytic cell area to analyze the distribution of chemicals in the proposed matrix. The method was tested in laboratory scale experiments where the CCA contaminated wood chips and clean sawdust were placed in various places of the cell, i.e., adjacent to the cathode and anode compartments and in the mid-section of the cell. The duration of each experiment was 14 days and the current was kept constant at 40 mA. During the electrodialytic treatment negative copper and chromium compounds were mostly found in the anolyte or accumulated in the anion-exchange membrane. The amount of positive compounds found in the catholyte is negligible. The placement of contaminated chips close to the anode and cathode showed that copper and chromium moved towards the positively charged anode. If placed in the mid section of the cell, significant amount of contaminants remained bound to the wood matrix or slightly migrated towards the anode. To predict the formation of copper and chromium species at certain pH and Eh, the computer-modeling program MTDATA to generate Pourbaix diagrams was used. 相似文献
34.
35.
36.
Dorigo B Schalch T Kulangara A Duda S Schroeder RR Richmond TJ 《Science (New York, N.Y.)》2004,306(5701):1571-1573
Chromatin folding determines the accessibility of DNA constituting eukaryotic genomes and consequently is profoundly important in the mechanisms of nuclear processes such as gene regulation. Nucleosome arrays compact to form a 30-nanometer chromatin fiber of hitherto disputed structure. Two competing classes of models have been proposed in which nucleosomes are either arranged linearly in a one-start higher order helix or zigzag back and forth in a two-start helix. We analyzed compacted nucleosome arrays stabilized by introduction of disulfide cross-links and show that the chromatin fiber comprises two stacks of nucleosomes in accord with the two-start model. 相似文献
37.
Herd CD Blinova A Simkus DN Huang Y Tarozo R Alexander CM Gyngard F Nittler LR Cody GD Fogel ML Kebukawa Y Kilcoyne AL Hilts RW Slater GF Glavin DP Dworkin JP Callahan MP Elsila JE De Gregorio BT Stroud RM 《Science (New York, N.Y.)》2011,332(6035):1304-1307
The complex suite of organic materials in carbonaceous chondrite meteorites probably originally formed in the interstellar medium and/or the solar protoplanetary disk, but was subsequently modified in the meteorites' asteroidal parent bodies. The mechanisms of formation and modification are still very poorly understood. We carried out a systematic study of variations in the mineralogy, petrology, and soluble and insoluble organic matter in distinct fragments of the Tagish Lake meteorite. The variations correlate with indicators of parent body aqueous alteration. At least some molecules of prebiotic importance formed during the alteration. 相似文献
38.
Alexandra M. Shultz Marianne H. Begemann Denise A. Schmidt Kathleen C. Weathers 《Water, air, and soil pollution》1993,69(1-2):113-125
The purpose of this work was to characterize the Al chemistry along the longitudinal pH gradient of the Coxing Kill and thereby provide a basis for an in depth study of aluminum chemistry and transport as a function of pH. Water samples were collected along the pH gradient and analyzed for monomeric Al species, strong acid anions, base cations, ammonium, dissolved organic C and dissolved silica. Inorganic monomeric Al, organic momonmeric Al and dissolved organic C decreased in concentration as the stream's drainage area and pH increased. The ratio of organic to inorganic monomeric Al and the concentration of all other species increased with increasing pH. An abrupt change in stream chemistry occurred in conjunction with the gradual changes expected for a low order stream draining an acid sensitive watershed impacted by acid deposition. This abrupt change partially resulted from geological characteristics of the watershed. 相似文献
39.
Advanced solid phase extraction using molecularly imprinted polymers for the determination of quercetin in red wine 总被引:5,自引:0,他引:5
Solid phase extraction (SPE) based on molecularly imprinted polymers (MIPs) is a novel approach for sample preparation and preconcentration, gaining increased interest in the fields of environmental, clinical, and food analysis. The first application combining MIPs with SPE for advanced beverage analysis is reported. MIPs for the flavonoid quercetin have been generated, using quercetin as a template molecule in a self-assembly approach and yielding imprinting of 1% of the used template. The MIP achieved a capacity of 0.4 g quercetin per gram polymer and a recovery rate of 98.2%. The application of these synthetic receptors as SPE material for the selective extraction and preconcentration of quercetin from synthetic and red wine samples was investigated. Red wine samples from a French Merlot were directly applied onto the SPE cartridge. The collected fractions were analyzed by high-pressure liquid chromatography. For verification of the obtained results, a similarly prepared nonimprinted polymer and a classical octadecyl silane reversed-phase cartridge were applied as the SPE matrix during control experiments. The MIP enabled the selective extraction of quercetin from a complex matrix, such as red wine, spiked with 8.8 mg per liter quercetin, demonstrating the potential of molecularly imprinted solid phase extraction for rapid, selective, and cost-effective sample pretreatment. 相似文献
40.
Reinhard A. Hirt Alexandra Galler Sarina Shibly Andrea Bilek 《Veterinary journal (London, England : 1997)》2011,187(1):54-59
Airway hyperresponsiveness is a key feature of human asthma and chronic bronchitis and response to the indirectly acting agonist adenosine 5′-monophosphate (AMP) is thought to reflect underlying airway inflammation. To examine whether airway responsiveness testing (ART) with AMP may be used to differentiate healthy cats from those with asthma (FA) and chronic bronchitis (CB), 24 cats (9 FA, 6 CB, 9 controls) underwent ART with AMP at concentrations of 0.1, 1, 10, 100 and 500 mg/mL using barometric whole body plethysmography.The defined endpoint of ART, an increase in enhanced pause (Penh) exceeding 300% of the post-saline value (baseline), was reached in 9/15 patients (7 FA, 2 CB), but in none of the controls. Mean Penh (±SD) at baseline (BL) was 0.49 ± 0.16 for cases, and 0.54 ± 0.16 for controls, and was significantly increased after AMP challenge in clinical cases (2.62 ± 2.20), but not in controls (0.63 ± 0.30, P < 0.05). After separating responder (R) and non-responder (NR) cases, a more pronounced difference after challenge was found (R: 3.96 ± 1.84, NR: 0.6 ± 0.21, P < 0.001). The provocative concentration of the agonist that increased Penh to 300% of BL (PC Penh 300) in R cases was 52.98 ± 48.04 mg/mL AMP. Age had no influence on the responder status or PC Penh 300. It was concluded that AMP challenge may offer a new method for the identification of cats with lower inflammatory airway disease, and possibly for monitoring disease progression or response to therapy. 相似文献