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161.
Laura D Brown Stephanie R Thorn Alex Cheung Jinny R Lavezzi Frederick C Battaglia Paul J Rozance 《畜牧与生物技术杂志(英文版)》2014,5(1):28
Background
The importance of non-glucose carbohydrates, especially mannose and inositol, for normal development is increasingly recognized. Whether pregnancies complicated by abnormal glucose transfer to the fetus also affect the regulation of non-glucose carbohydrates is unknown. In pregnant sheep, maternal insulin infusions were used to reduce glucose supply to the fetus for both short (2-wk) and long (8-wk) durations to test the hypothesis that a maternal insulin infusion would suppress fetal mannose and inositol concentrations. We also used direct fetal insulin infusions (1-wk hyperinsulinemic-isoglycemic clamp) to determine the relative importance of fetal glucose and insulin for regulating non-glucose carbohydrates.Results
A maternal insulin infusion resulted in lower maternal (50%, P < 0.01) and fetal (35-45%, P < 0.01) mannose concentrations, which were highly correlated (r2 = 0.69, P < 0.01). A fetal insulin infusion resulted in a 50% reduction of fetal mannose (P < 0.05). Neither maternal nor fetal plasma inositol changed with exogenous insulin infusions. Additionally, maternal insulin infusion resulted in lower fetal sorbitol and fructose (P < 0.01).Conclusions
Chronically decreased glucose supply to the fetus as well as fetal hyperinsulinemia both reduce fetal non-glucose carbohydrates. Given the role of these carbohydrates in protein glycosylation and lipid production, more research on their metabolism in pregnancies complicated by abnormal glucose metabolism is clearly warranted. 相似文献162.
Steffan Edward Octávio Oliveira Cíntia Carol de Melo Costa João Batista Freire de Souza Jr João Paulo Araújo Fernandes de Queiroz Alex Sandro Campos Maia Leonardo Lelis de Macedo Costa 《Tropical animal health and production》2014,46(8):1413-1417
The amount of short-wave solar radiation willingly tolerated by lactating Holstein cows on pasture was determined in an equatorial semi-arid environment. The study was carried out on a dairy farm located in Limoeiro do Norte, CE, northeastern Brazil. The observed behaviours were as follows: grazing, under the sun, under the shade, standing, lying, ruminating, idling and wallowing in the water. The behaviours were recorded using instantaneous scan sampling at regular intervals of 15 min from 0600 to 1800 hours over 5 days. On all sampling days, the meteorological variables, including local short-wave solar radiation (R S-W, W m?2), were recorded. The R S-W data were divided into five levels. The sun exposure was more frequent under low (100 %) and moderately low (97 %) levels, when R S-W remained below 500 W m?2. The grazing was more intense under low (100 %) and moderately low (93 %) levels. Above 500 W m?2, the grazing time significantly decreased (11 %). The cows avoided grazing under high (0 %) and very high (0 %) levels, when R S-W exceeded 700 W m?2. The ruminating behaviour was more frequent under high (33 %) and very high (37 %) levels, in which the highest averages of R S-W were recorded (815 and 958 W m?2, respectively). The standing posture was more frequent under low (100 %) and moderately low (97 %) levels. Therefore, the critical R S-W level that motivates cows to stop grazing and seek shade was in the interval between 500 and 700 W m?2. 相似文献
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164.
Response of healthy dogs to infusions of human serum albumin 总被引:1,自引:0,他引:1
Cohn LA Kerl ME Lenox CE Livingston RS Dodam JR 《American journal of veterinary research》2007,68(6):657-663
OBJECTIVE: To evaluate the clinical and immunologic response in healthy dogs to infusions of human serum albumin (HSA). ANIMALS: 9 healthy purpose-bred mixed-breed dogs. PROCEDURES: Each dog was administered a 25% HSA solution once or twice. Various physical examination and laboratory variables were serially evaluated. Antibody against HSA was assayed before and after infusion by use of an ELISA. Intradermal testing was also conducted. A repeated-measures ANOVA or Friedman repeated-measures ANOVA on ranks was used to compare results for the variables. RESULTS: Adverse clinical reactions were observed after the first or second infusion in 3 dogs. Anaphylactoid reactions were observed in 1 of 9 dogs during the first infusion and in 2 of 2 dogs administered a second infusion. Two dogs developed severe edema and urticaria 6 or 7 days after an initial infusion. All dogs developed anti-HSA antibodies. Positive responses for ID tests were observed in 8 of 9 dogs. Short-term increases were detected in blood protein, total bilirubin, and calcium concentrations after HSA infusion. Serum cholesterol concentrations and platelet counts decreased after HSA infusion. CONCLUSIONS AND CLINICAL RELEVANCE: Administration of HSA resulted in profound reactions in 2 of 9 dogs administered a single infusion and in 2 of 2 dogs administered a second infusion. This indicates that there is risk of life-threatening adverse reactions to HSA infusion in healthy dogs. 相似文献
165.
McElroy SM Szalanski AL McKay T Bermudez AJ Owens CB Steelman CD 《Veterinary parasitology》2005,127(2):165-168
A 1520 bp region of Cochlosoma anatis mtDNA 16S gene was subjected to DNA sequencing and a 466 bp portion was compared with other protozoan 16S sequences to develop PCR primers specific for C. anatis. This PCR diagnostic method allowed identification of C. anatis from house flies, Musca domestica L., turkey gut, and fecal samples within 6 h after field-collected samples reached the laboratory. House flies detected carrying C. anatis using the diagnostic 374 bp amplicons represented the first record of this protozoan in house flies. 相似文献
166.
L. R. R. Costa C. G. Donelly C. M. Crowe B. Vaughan C. E. Alex M. Aleman 《Equine Veterinary Education》2020,32(5):238-245
The focus of this report was to document the electroencephalogram (EEG) findings associated with nonislet cell tumour-induced hypoglycaemia (NICTH) in a racing Thoroughbred mare subsequently diagnosed with renal carcinoma and carcinomatosis. We evaluated cortical cerebral activity and confirmed paroxysmal activity consistent with subclinical seizures by performing an EEG prior to onset of clinical signs of seizures. We showed that the electrical activity pattern progressed in a similar fashion as in humans with severe, progressive hypoglycaemia and presumed neuroglycopenia. The glycaemic threshold for abnormalities detected in EEG showed that abnormal electrical cortical activity appeared when blood glucose dropped to 44 mg/dL (2.4 mmol/L) and paroxysmal activity spread, becoming generalised when blood glucose dropped to 23 mg/dL (1.3 mmol/L). NICTH is an uncommon paraneoplastic phenomenon in several species. In horses, NICTH has been reported most frequently in primary renal tumours, followed by primary hepatic tumours. This report highlights the importance of investigating the possibility of hypoglycaemia in cases of horses that present altered state of consciousness with or without intermittent seizures. This case report also highlights that the diagnostic work-up for horses presenting with episodic hypoglycaemia should comprise investigation of possible neoplasia, as paraneoplastic hypoglycaemia should be included in the list of differentials of horses with hypoglycaemia. 相似文献
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Jay L. Haddock Ray D. Jackson W. O. Harrington R. L. Olson W. J. Weston Mary L. Belote W. J. Hooker A. P. Benson R. W. Hougas S. J. Poloquin R. W. Ross G. V. C. Houghland Wm. G. Hoyman D. R. Isleib N. R. Thompson T. Miyamoto E. J. Wheeler S. T. Dexter Hugh J. Murphy Michael Goven Alvah L. Perry G. H. Rieman D. B. Robinson R. H. Larson J. G. Walker R. L. Sawyer Lawrence A. Schaal Clark H. Livingston Ora Smith W. L. Smith Vilhelm Umaerus L. M. Ware W. A. Johnson R. E. Webb E. S. Schultz Donald A. Young L. C. Young 《American Journal of Potato Research》1958,35(3):441-449