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21.
Spatial genetic structure and dispersal of the cacao pathogen Moniliophthora perniciosa in the Brazilian Amazon
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A. S. Artero J. Q. Silva P. S. B. Albuquerque E. A. Bressan G. A. Leal Jr A. M. Sebbenn G. W. Griffith A. Figueira 《Plant pathology》2017,66(6):912-923
Moniliophthora perniciosa is the causal agent of witches’ broom in Theobroma cacao (cacao). Three biotypes of M. perniciosa are recognized, differing in host specificity, with two causing symptoms on cacao or Solanaceae species (C‐ and S‐biotypes), and the third found growing endophytically on lianas (L‐biotype). The objectives of this study were to clarify the genetic relationship between the three biotypes, and to identify those regions in the Brazilian Amazon with the greatest genetic diversity for the C‐biotype. Phylogenetic reconstruction based on the rRNA ITS regions showed that the C‐ and S‐biotypes formed a well‐supported clade separated from the L‐biotype. Analysis of 131 isolates genotyped at 11 microsatellite loci found that S‐ and especially L‐biotypes showed a higher genetic diversity. A significant spatial genetic structure was detected for the C‐biotype populations in Amazonia for up to 137 km, suggesting ‘isolation by distance’ mode of dispersal. However, in regions containing extensive cacao plantings, C‐biotype populations were essentially ‘clonal’, as evidenced by high frequency of repeated multilocus genotypes. Among the Amazonian C‐biotype populations, Acre and West Amazon displayed the largest genotypic diversity and might be part of the centre of diversity of the fungus. The pathogen dispersal may have followed the direction of river flow downstream from Acre, Rondônia and West Amazon eastward to the rest of the Amazon valley, where cacao is not endemic. The Bahia population exhibited the lowest genotypic diversity, but high allele richness, suggesting multiple invasions, with origin assigned to Rondônia and West Amazon, possibly through isolates from the Lower Amazon population. 相似文献
22.
OLIVER M. CROWE BVSc Cert ES RICHARD J. HEPBURN BVSc Cert EM Diplomate ACVIM SVEND E. KOLD DVM PhD ROGER K. SMITH MA Vet MB PhD DEO Diplomate ECVS 《Veterinary surgery : VS》2010,39(1):107-114
Objective— To report long-term outcome after arthroscopic removal of fragmentation of the extensor process of the distal phalanx in horses.
Study Design— Case series.
Animals— Adult horses (n=13).
Methods— Medical records (2003–2004) of horses that had arthroscopic debridement of fragmentation of the extensor process of the distal phalanx were reviewed. Inclusion criteria included: lameness localized to the foot, fragmentation of the extensor process of the distal phalanx debrided arthroscopically, and a follow-up period of ≥4 years.
Results— Of the 13 horses, lameness was resolved in 11 (85%) initially but distal interphalangeal joint pain recurred in 2 (15%) within 1 year of surgery. Three (23%) other horses were retired because of lameness at other sites during the follow-up period resulting in 46% (6/13) being not lame and in full work after 4 years. Substantial changes were identified at surgery in the contralateral joint of 7 horses, even when fragments were only present unilaterally.
Conclusions— Arthroscopic debridement of fragmentation of the extensor process of the forelimb distal phalanx has a good short-term prognosis for resolution of lameness and return to work but a more guarded prognosis for long-term soundness.
Clinical Relevance— These results allow for more accurate prognostication preoperatively and may support early debridement of fragmentation of the extensor process of the distal phalanx. 相似文献
Study Design— Case series.
Animals— Adult horses (n=13).
Methods— Medical records (2003–2004) of horses that had arthroscopic debridement of fragmentation of the extensor process of the distal phalanx were reviewed. Inclusion criteria included: lameness localized to the foot, fragmentation of the extensor process of the distal phalanx debrided arthroscopically, and a follow-up period of ≥4 years.
Results— Of the 13 horses, lameness was resolved in 11 (85%) initially but distal interphalangeal joint pain recurred in 2 (15%) within 1 year of surgery. Three (23%) other horses were retired because of lameness at other sites during the follow-up period resulting in 46% (6/13) being not lame and in full work after 4 years. Substantial changes were identified at surgery in the contralateral joint of 7 horses, even when fragments were only present unilaterally.
Conclusions— Arthroscopic debridement of fragmentation of the extensor process of the forelimb distal phalanx has a good short-term prognosis for resolution of lameness and return to work but a more guarded prognosis for long-term soundness.
Clinical Relevance— These results allow for more accurate prognostication preoperatively and may support early debridement of fragmentation of the extensor process of the distal phalanx. 相似文献
23.
Studies on the effect of forest loss and fragmentation on plant species richness at different spatial scales have yielded contradictory results that have been attributed to different ways to measure fragmentation. The main goal of this study was to investigate the independent and combined effects of forest loss and habitat fragmentation on woody species richness. Woody species were grouped according to their habitat requirements (forest specialists, forest generalists, non-forest and total woody species). We used regression models to investigate the effect of fragmentation and human occupation on woody species richness. The underlying factors were investigated by partitioning of the variation, i.e. decomposing the variation in species richness between the pure effects of forest loss, fragmentation and human occupation. The relationship between species richness and forest loss resulted extremely non-linear. The models for forest specialist, generalist and total woody species richness accounted for 35%, 31% and 33% of the total variance respectively. Model for non-forest species richness only accounted for 7% of the total variance. The largest fraction of variability in species richness was accounted by fragmentation variables for all groups (except for non-forest species). Results emphasize the larger independent effect of fragmentation over forest loss, suggesting that species variation is mainly conditioned by the spatial configuration of the habitat. 相似文献
24.
25.
The interest in the effect of genotype × environment interaction is increasing because animal breeding programs have become geographically broader. Climate changes in the next decades are also expected to challenge the present breeding goals, increasing the importance of environmental sensitivity. The aim of this work was to analyze genotype × environment interaction effect on cattle BW using the environmental sensitivity predicted by random regression reaction norm models, including sex and age effects as additional dimensions in the study. Genetic parameters were estimated for adjusted BW of Brazilian Nelore cattle at different ages (120, 210, 365, and 450 d), using linear polynomials for random regression analysis. The analyses with sex as a fixed effect (total analyses) were compared with those with sex-separated progenies (male and female progeny analyses, respectively). (Co)variance components were estimated and breeding values calculated EPD. The results showed important differences in reaction norm model genetic parameter estimates according to different age and sex analyses. The results confirmed the presence of an important genotype × environment × sex × age interaction for Nelore cattle BW. The patterns in these results lead to a revision of the importance of sexual and developmental factors on plasticity and adaptation concepts. 相似文献
26.
Thaise Pinto de Melo Marina Rufino Salinas Fortes Ben Hayes Lucia Galvão de Albuquerque Roberto Carvalheiro 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(2):139-154
The aim of this study was to identify candidate regions associated with sexual precocity in Bos indicus. Nellore and Brahman were set as validation and discovery populations, respectively. SNP selected in Brahman to validate in Nellore were from gene regions affecting reproductive traits (G1) and significant SNP (p ≤ 10–3) from a meta-analysis (G2). In the validation population, early pregnancy (EP) and scrotal circumference (SC) were evaluated. To perform GWAS in validation population, we used regression and Bayes C. SNP with p ≤ 10–3 in regression and Bayes factor ≥3 in Bayes C were deemed significant. Significant SNP (for EP or SC) or SNP in their ±250 Kb vicinity region, which were in at least one discovery set (G1 or G2), were considered validated. SNP identified in both G1 and G2 were considered candidate. For EP, 145 SNP were validated in G1 and 41 in G2, and for SC, these numbers were 14 and 2. For EP, 21 candidate SNP were detected (G1 and G2). For SC, no candidate SNP were identified. Validated SNP and their vicinity region were located close to quantitative trait loci or genes related to reproductive traits and were enriched in gene ontology terms related to reproductive success. These are therefore strong candidate regions for sexual precocity in Nellore and Brahman. 相似文献
27.
Iara Del Pilar Solar Diaz Gregório Miguel Ferreira de Camargo Valdecy Aparecida Rocha da Cruz Isis da Costa Hermisdorff Caio Victor Damasceno Carvalho Lucia Galvão de Albuquerque Raphael Bermal Costa 《Reproduction in domestic animals》2020,55(11):1650-1654
Although the second largest chromosome of the genome, the X chromosome is usually excluded from genome-wide association studies (GWAS). Considering the presence and importance of genes on this chromosome that are involved in reproduction, the aim of this study was to evaluate the effect of its inclusion in GWAS on reproductive traits (scrotal circumference [SC], early pregnancy [P16] and age at first calving [AFC]) in a Nelore herd. Genotype data from 3,263 animals with the above-mentioned phenotypes were used. The results showed an increase in the variances explained by the autosomal markers for all traits when the X chromosome was not included. For SC, there was an increase of more than 10% for the windows on chromosomes 2 and 6. For P16, the effect was increased by almost 20% for windows on chromosome 5. The same pattern was found for AFC, with an increase of more than 10% for the most important windows. The results indicate that the noninclusion of the X chromosome can overestimate the effects of autosomes on SC, P16 and AFC not only because of the additive effect of the X chromosome itself but also because of its epistatic effect on autosomal genes. 相似文献
28.
Soares Camila Rocha Júnior Vicente Ribeiro Monção Flávio Pinto Borges Lucas Daniel Alcântara Caldeira Luciana Albuquerque Costa Natanael Mendes Ruas José Reinaldo Mendes Rigueira João Paulo Sampaio da Cunha Siqueira Carvalho Cinara de Sales Eleuza Clarete Junqueira de Assis Pires Daniel Ananias Chamone Julieta Maria Alencar 《Tropical animal health and production》2020,52(5):2567-2576
Tropical Animal Health and Production - The qualities of food, mainly of animal origin, have always been of concern to consumers. It is known that the composition of animals’ diets can... 相似文献
29.
H. Tonhati F. B. Vasconcellos L. G. Albuquerque 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2000,117(5):331-336
This study investigated genetic trends of some productive and reproductive traits in a herd of Murrah buffalo raised in São Paulo, Brazil. Variance components for milk production (MP), length of lactation (LL), calving interval (CI) and age of first calving (AFC) were estimated by the restricted maximum likelihood method, using an animal model. Estimated heritability values were 0.38; 0.01; 0.10 and 0.20 for MP, LL, CI and AFC, respectively. Estimated repeatability values were 0.50, 0.13 and 0.20 for MP, LL and CI, respectively. Means of predicted breeding values for cows, dams and sires according to calving year and the genetic correlations were presented. 相似文献
30.
Vanessa MILECH Pmela CAYE Bernardo NASCIMENTO ANTUNES Josaine Cristina da SILVA RAPPETI Soliane CARRA PERERA Martielo Ivan GEHRCKE Thaís Cozza dos SANTOS Camila CONTE Fabrício de VARGAS ARIGONY BRAGA Guilherme Albuquerque de OLIVEIRA CAVALCANTI Maurício VELOSO BRUN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):618
This paper reports on two cases of laparoscopic nephrotomy employed in the treatment of canine dioctophymosis, which is considered a unusual procedure and a new treatment proposal heretofore not performed in veterinary medicine. Two patients were treated, one with a history of hematuria and the other with incidental finding of the parasite in the abdominal cavity during elective ovariohysterectomy. Both dogs were subjected to abdominal ultrasound, which produced images indicating the presence of the parasite in the right side kidney, but with partial parenchymal preservation. The patients were therefore subjected to laparoscopic nephrotomy. The surgical procedure was effective in treating dioctophymosis and enabled minimum tissue invasion during surgery, in addition to preservation of the kidney. 相似文献